• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.2
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• pp.127-132
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• 1999

No comprehensive study of yield and quality losses of forage legumes harvested and cured in spring has been conducted in Korea, therefore, this experiment was carried out to gain information on yield and quality, losses of alfalfa(Medicgo sativa L.), crimson clover (Trifolium incarnatum L.), and red clover(Trifolium pratense L.) during field curing in spring(mid-May). Alfalfa was highest in dry matter content of 15% at harvest, while crimson clover and red clover had 12.4 and 11.5%, respectively. Species differences for the dry matter content were maintained and consistent trends were observed during the field curing. Alfalfa took seven days to reach dry matter content over 80%, while both crimson clover and red clover took eight days. Crimson clover and alfalfa tended to show a higher leaf-stem ratio than red clover based on dry matter, but red clover showed a higher leaf-stem ratio than alfalfa and crimson clover based on crude protein content. Losses in dry matter of alfalfa from leaf shattering were higher than crimson clover and red clover, and similar trends were detected losses in crude protein for three different legumes. Fresh legume forages averaged higher in clude protein(CP), total digestible nutrients(TDN), and relative feed value(RFV), while dry legume forages averaged lower in acid detergent fiber(ADF) and neutral detergent fiber(NDF). Results of the experiment indicate that hay curing in spring affects crude protein concentration more than ADF and NDF, and this is due, in part, to leaf shattering caused by field operations.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.5
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• pp.649-660
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• 2005

The effects of Lactobacillus buchneri 40788 and buffered propionic acid on preservation, intake and digestibility of alfalfa (Medicago sativa) and timothy (Phleum pratense) hay were investigated. During baling, forages were treated with L. buchneri 40788 (1.2${\times}$10$^6$ CFU/g) as a liquid (LLB) or as a granular preparation (GLB), with buffered propionic acid (10 mL/kg, BPA), or left untreated (control). Triplicate 500 kg round bales of each treatment were put up at two moisture levels for each forage: 17%${\pm}$0.33% and 20%${\pm}$0.30% for timothy and 17%${\pm}$0.20% and 19%${\pm}$0.27% for alfalfa (mean${\pm}$SD). Bales were sampled for chemical and microbiological analyses after 0, 30 and 60 d of storage. Compared to controls, all preservatives reduced (p<0.05) heating of both forages at all moisture levels with the exception of alfalfa baled at 19% moisture. After 60 d of storage, GLB reduced (p<0.05) moulds in 17% timothy hay as compared to other treatments, but at 20% moisture, moulds were reduced in LLB- and BPA-treated timothy as compared to controls. In alfalfa at 17% moisture, total bacteria were lower (p<0.05) in GLB-treated bales than LLB or control bales, but yeast and total bacteria were only reduced in BPA-treated alfalfa at 19% moisture. In situ DM disappearance of timothy (both moisture levels) and alfalfa (19% moisture level) increased (p<0.05) with LLB treatment compared to control. Digestibility of both forages did not differ (p>0.05) among treatments, however, voluntary DM intake of LLB-treated timothy (1.32 kg/d) was 22.3% higher (p<0.05) than control, and 14.1% higher than BPA-treated timothy. Treating timothy and alfalfa hay with L. buchneri 40788 or buffered propionic acid may improve the nutritive value of the hay when baled at 17 to 20% moisture.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.293-304
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• 1997

A field experiment was conducted at the forage experimental plots, Seoul National University, Suweon h m 1995 to 1996 to determine the effect of legume residues as a N source and N fertilizer on corn (Zea mays L.) silage yield, N uptake, and availability of inorganic N in the soil. Corn was grown following (i) red clover (Trifolium pratense L.), (ii) crimson clover (Trifolium incarnatum L.), (iii) alfalfa (Medicago sativa L.) and (iv) winter fallow. The plots were split into two rates of fertilizer N (0 and 90kg Nlha) in a split-plot experimental plan. Compared with fallow treatment, legumes depleted soil water in the surface 15cm at corn planting by 17 to 26%. As a result, corn emergence was markedly delayed with legume residues by 8 to 11 days. Corn silage DM yield was significantly reduced in the presence of legume residues by 2.0 to 3.4 and 1.5 to 2.5 tonlha compared with winter fallow treatment at 0 and 90kg fertilizer Nlha, respectively, but no significant difference in the corn DM yield was found between legume residue treatments. There was an overall tendency for increased corn yields with 90kg fertilizer Nlha compared to Okg fertilizer Nlha, although not all yield increases were significantly greater. The corn yield response to applied N suggests that a source of N from legume residues was not sufficient for a succeeding corn crop. There was significantly more N (P

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.630-635
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• 2000

Previous research has not evaluated the effects of various rates and frequencies of manure application and frequencies of cutting on yield and stand persistence of cool-season grasses and alfalfa-grass mixtures. The primary objective of this study was to compare the effects of cutting management systems on herbage yield and stand persistence of orchardgrass (Dactylis glomerata L.) and an alfalfa (Medicago sativa L.)-orchardgrass mixture from various rates and frequencies of dairy slurry application. A randomized complete block design with treatments in a sub-subplot arrangement with four replicates was used. The main plot consisted of 2 cutting management systems (4 and 5 annual cuttings). The subplots were 9 fertility treatments: 7 slurry rate and frequency of application treatments, one inorganic fertilizer treatment, and an unfertilized control. The split-split-plots were the two forage species: orchardgrass and alfalfa-orchardgrass mixture. The study was initiated after 1st cutting in 1995. Cumulative yields of the 2nd and subsequent cuttings of both orchardgrass and alfalfa-orchardgrass in 1995 were higher for the 5-cutting system than the 4-cutting system. The 1995 growing season was abnormally dry. In 1996, an abnormally wet year, the reverse was true, total herbage yields being higher for the 4-cutting system than the 5-cutting system. Species response to fertility rate/frequency treatments was different in both years. Higher application rates early in the season and carryover of nutrients from late season applications the previous year appear to be responsible for the yield increases of those fertility treatments having significant yield differences between the cutting management systems. The stand ratings of orchardgrass were not affected by cutting management. In the spring of 1997, however, the stand ratings of alfalfa-orchardgrass in the 4-cutting management system were significantly greater than the 5-cutting management system. The very high manure application rate significantly reduced the stand ratings of alfalfa-orchardgrass in the 5-cutting system.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1524-1528
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• 2004

In order to determine the extent of change in nitrogen fractions and in vitro ruminal degradability of forage protein during ensilage and the influence on nitrogen utilization by sheep, orchardgrass (Dactylis glomerata L.) and alfalfa (Medicago sativa L.) were ensiled in separate 120 L silos for 5, 21 and 56 days. With respect to nitrogen fractions, fraction 1 (buffer solution soluble nitrogen), fraction 2 (buffer solution insoluble nitrogen-neutral detergent insoluble nitrogen), fraction 3 (neutral detergent insoluble nitrogen-acid detergent insoluble nitrogen), and fraction 4 (acid detergent insoluble nitrogen) were determined. Fractions 1 and 2 accounted for more than 80% of total nitrogen in orchardgrass and 90% of that in alfalfa. The proportion of fraction 1 in orchardgrass increased from 33.0% at day 0 to 52.0% after day 56 of ensiling. In the case of alfalfa silage it was 41.7% and 62.9%, respectively. Seventy percent of this increase occurred within the first 5 days of ensiling. A similar change of in vitro ruminal degradability of total nitrogen was also observed in both forages. Nitrogen retention in sheep tended to decrease as the length of ensiling increased, with a significantly positive correlation between urinary nitrogen and fraction 1, and in vitro ruminal degradability of total nitrogen.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.84-89
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• 1991

This experiment was carried out to determine the effects of oversowing and other seeding methods (oversowing+ raking, oversowing+ raking+compaction, tillage+ broadcasting+ compaction and tillage + drilling+ compaction) on growth, dry matter and crude protein yield of alfalfa (Medicago satiua L.). The results obtained are summarized as follows:1. Soil pH and soil properties were improved by tillage.2. Establishment of alfalfa was increased with raking and compaction, and more by tillage than by oversowing(P

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.534-540
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• 2003

This study was conducted to investigate the effect of supplementation of galacto-oligosaccharides (GOS) on methane emission, nutrient digestibility, energy utilization and blood metabolites by Holstein cows fed silages. In two sequential digestion and respiratory trials, two non-lactating Holstein cows were arranged to a balanced incomplete block design. Experimental diets consisted of two silage types; orchardgrass (Dactylis glomerata L.) based silage (OS), mixed silage (orchardgrass based silage and alfalfa (Medicago sativa L.) silage) (MS), while two GOS levels were without supplementation (0) and 2% of dry matter intake supplementation (2). Four combination diets were OS-0, OS-2, MS-0 and MS-2. Significant effects of silage types and GOS supplementation levels were not observed for DM and OM intake. Whereas the digestibility of OM, NDF and ADF was significantly (p<0.05) higher in cows fed OS with and without GOS compared cows fed MS diets. As percentage of GE intake, fecal energy loss for OS diets was significantly (p<0.05) declined than for MS diets. In contrast, cows fed MS diets had lower (p<0.05) urine energy loss as a proportion of GE intake compared to OS diets. Energy loss as CH4 and heat production was numerically increased when cows fed both OS and MS with GOS supplementation. Compared to OS, CH4 emission in cows fed MS was numerically decreased by 10.8 %. Methane conversion ratio (energy loss as CH4 per unit of GE intake) for OS-0, OS-2, MS-0 and MS-2 were 7.1, 7.2, 6.8 and 7.0, respectively. Plasma of glucose and urea-N concentration were significantly (p<0.05) elevated from 1 h to 6 h after feeding, otherwise total protein in plasma was declined (p<0.01) at 6 after feeding.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.245-252
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• 1995

To select a carrier as Rhizobiwn inoculants for alfalfa, severeal Rhizobium inoculants were produced by adding or not sub-materials($CaCO_3$ and sucrose) to main materials(organic or inorganic materials). The root nodule bacteria, Rhizobium meliloti 1061 distributed from KAIST, and Vemal alfalfa was used in this experiment. The Rhizobium populations and inoculation effects of the Rhizobium inoculants produced in several materials were scrutinized at laboratory and greenhouse in Livestock Experiment Station, RDA from 1993 to 1994. Moisture contents of the caniers were varied from 32 to 50% on dry weight basis according to material characteristics and the pH ranges of these were varied from pH 4.56 to 10.06 according to raw material characteristics and preparations. Initial Rhizobium numbers of the carriers were higher in organic material-inoculants than in inorganic, and among the inoculants, the inoculant made of Bentonite+Vermiculite(l:3 w/w) was excellent because of high rhizobium population($7.8~8.3\times10^8/g$ inoculant) and high rhizobium reappearance of inoculant in severed different production time. The root nodules of the alfalfa inoculated with different inoculants were fast formed in the fermented sawdust with cattle dung (FSC) inoculant, and bentonite(B)+vermiculite(V) than others. Plant length of alfalfa was differentiated on 15 days after inoculation but was not nearly different between higher inoculants than rhizobium number $10^7/g$ inoculant. Total dry matter of alfalfa was yielded by 20.65, 20.34mg per pot in FSC + sucrose 0.5% and B + V + sucrose 1% inoculants respectively that were higher inoculation effect by 17 times compared with non-inoculation, 1.2 mg per pot.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.253-258
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• 1995

'Ihis experiment was carried out to select a carrier as rhizobium inoculant for alfalfa. The Rhizobium inoculants were produced in main materials(organic and inorganic materials) and submaterial(Ca$CO_3$, and sucrose), and than the changes of Rhizobium population of inoculants were scrutinized from directly after the production to 12 months at $4^{\circ}$C and $25^{\circ}$C. Directly after the production of inoculants, their Rhizobium numbers were 4-9 $\times$ $10^9$/ginoculant in organic materials and 1-8 $\times$ $10^8$/g inoculant in inorganic materials. When they were stored at $25^{\circ}$C, most of inoculants was being started in reduction of rhizobium numbers after 2 months and was reduced by tenfold of initial rhizobium number after 4-6 months, but inoculant of bentonite+ vermiculite+sucrose 1% was not reduced by tenfold after 12 months. When they were stored at $4^{\circ}$C, most of inoculants was being started in reduction of rhizobium numbers after 4 months but was not reduced by tenfold of initial rhizobium number after 12 months, and the effect of sucrose addition to bentonite+ vermiculik did nearly not appear. Preservation of Rhizobium inoculants was more effective at 4C than 25 C . 'Ihe Rhizobium inoalant produced in bentonite+vermiculite(l:3 w/w)+ sucrose 1% was not only high Rhizobium population(8.3 $\times$ $10^8$/g inoculant) but also survival effect of rhizobium in being stored at $25^{\circ}$C.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.228-236
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• 2004

Two varieties of alfalfa (Medicago sativa L cv. Pioneer and Beaver) and timothy (Phleum pratense L cv. Climax and Joliette), grown at different locations in Saskatchewan (Canada), were cut at three stages [1=one week before commercial cut (early bud for alfalfa; joint for timothy); 2=at commercial cut (late bud for alfalfa; pre-bloom head for timothy); 3=one week after commercial cut (early bloom for alfalfa; full head for timothy)]. The energy values of forages were determined using three approaches, including chemical (NRC 2001 formula) and biological approaches (standard in vitro and in situ assay). The objectives of this study were to determine the effects of forage variety and stage of maturity on energy values under the climate conditions of western Canada, and to investigate relationship between chemical (NRC 2001 formula) approach and biological approaches (in vitro and in situ assay) on prediction of energy values. The results showed that, in general, forage species (alfalfa vs. timothy) and cutting stage had profound impacts, but the varieties within each species (Pioneer vs. Beaver in alfalfa; Climax vs. Joliette in timothy) had minimal effects on energy values. As forage maturity increased, the energy contents behaved in a quadratic fashion, increasing at stage 2 and then significantly decreasing at stage 3. However, the prediction methods-chemical approach (NRC 2001 formula) and biological approaches (in vitro and in situ assay) had great influences on energy values. The highest predicted energy values were found by using the in situ approach, the lowest prediction value by using the NRC 2001 formula, and the intermediate values by the in vitro approach. The in situ results may be most accurate because it is closest to simulate animal condition. The energy values measured by biological approaches are not predictable by the chemical approach in this study, indicating that a refinement is needed in accurately predicting energy values.