• Title/Summary/Keyword: MeCP2

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Solution Dynamics and Crystal Structure of $CpMoOs_{3}(CO)_{10}(\mu-H)_{2}[\mu_{3}-\eta^{2}-C(O)CH_{2}Tol]$

  • Joon T. Park;Jeong-Ju Cho;Kang-Moon Chun;Sock-Sung Yun;Kim SangSoo
    • Bulletin of the Korean Chemical Society
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    • v.14 no.1
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    • pp.137-143
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    • 1993
  • The tetranuclear heterometallic complex CpMo$Os_3(CO)_{10}({\mu]-H)2[{\mu}3-{\eta}^2-C(O)CH_2Tol]\;(1,\;Cp={\eta}^5-C_5H_5,\;Tol=p-C_6H_4Me)$ has been examined by variable-temperature $^{13}$C-NMR spectroscopy and by a full three-dimensional X-ray structual analysis. Complex 1 crystallizes in the orthorhombic space group Pna2$_1$ with a = 12.960(1) ${\AA}$, b = 11.255(l) ${\AA}$, c = 38.569(10)${\AA}$, V = 5626(2) ${\AA}^3$ and ${\rho}$(calcd) = 2.71 gcm$^{-3}$ for Z = 8 and molecular weight 1146.9. Diffraction data were collectedon a CAD4 diffractometer, and the structure was refined to $R_F$ = 9.7% and $R_{W^F}$ = 9.9% for 2530 data (MoK${\alpha}$ radiation). There are two essentially equivalent molecules in the crystallographic asymmetric unit. The tetranuclear molecule contains a triangulated rhomboidal arrangement of metal atoms with Os(2) and Mo at the two bridgehead positions. The metal framework is planar; the dihedral angle between Os(l)-Os(2)-Mo and Os(3)-Os(2)-Mo planes is 180$^{\circ}$. A triply bridging (${\mu}_3,\;{\eta}^2$) acyl ligand lies above the Os(l)-Os(2)-Mo plane; the oxygen atom spans the two bridgehead positions, while the carbon atom spans one bridgehead position and an acute apical position. The molecular architecture is completed by an ${\eta}^5$-cyclopentadienyl ligand and a semi-triply bridging carbonyl ligand on the molybdenum atom, and nine terminal carbonyl ligands-four on Os(3), three on Os(l), and two on Os(2). The two hydride ligands are inferred to occupy the Os(l)-Os(2) and Mo-Os(3) edges from structural and NMR data.

Effect of Different Stocking Densities in Plastic Wired-Floor House on Performance and Uniformity of Korean Native Commercial Ducks (고상식 오리사에서 다양한 사육밀도가 토종 실용오리의 생산성 및 균일도에 미치는 영향)

  • Hong, Euichul;Kang, Bo-Seok;Kang, Hwan-Ku;Jeon, Jin-Joo;Kim, Hyun-Soo;Son, Jiseon;Kim, Chan-Ho
    • Korean Journal of Poultry Science
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    • v.46 no.4
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    • pp.215-221
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    • 2019
  • This study investigated the effects of various stocking densities on the growth performance of Korean native ducks. Twelve hundred day-old ducklings were reared for 50 days in a duck house (windowless, plastic wire-floor pen). Two weeks later, 852 of these ducks (350±30 g) were selected for at least 80% uniformity per stocking treatment (six treatments, four replications/treatment, depending on a stocking density of 4~9 birds m-2). Experimental diets were corn-soybean-based and broiler ducks were grouped based on age [0~21 days of age (CP 21%, ME 2,900 kcal/kg) and 21~50 days of age (CP 17%, ME 3,100 kcal/kg)]. Body weight (BW) did not significantly differ among different aged treatment groups (P>0.05); however, uniformity decreased with higher stocking densities (P<0.05). Feed intake and feed conversion ratio of ducks aged 42~50 days significantly decreased in the T4-6 treatments compared with the T1-3 treatments (P<0.05). In all experimental periods, BW gain was not significantly different among treatments, but feed intake decreased significantly as the stocking density increased (P<0.05), and feed conversion ratio was significantly different among six treatments with stocking density (P<0.05). In conclusion, we found that the stocking density of Korean native ducks in a plastic wire-floor pen was approximately 7 birds m-2 in consideration of productivity and uniformity.

Deriving Nutrient Requirements of Lactating Indian Cattle under Tropical Condition Using Performance and Intake Data Emanated from Feeding Trials Conducted in Different Research Institutes

  • Paul, S.S.;Mandal, A.B.;Mandal, G.P.;Kannan, A.;Pathak, N.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.769-776
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    • 2004
  • Data from 24 feeding trials conducted on lactating cattle from different institutes across India were subjected to regression analysis to derive requirements of ME, TDN, CP and DCP for maintenance, milk production and body weight gain. Maintenance requirements for ME, TDN, CP and DCP were 598 KJ, 39.5 g, 6.27 g and 2.90 g/kg $W^{0.75}$, respectively and the corresponding requirements for production of 1 kg 4% FCM were 5,023 KJ, 332 g, 82 g and 58 g. The corresponding requirements for one g gain in BW were 27 KJ, 1.78 g, 0.44 g and 0.19 g. Regression equations had high $R^{2}$ values (0.67 to 0.90) and the equations (Fvalue) as well as coefficients were highly significant (p<0.001). Regressed values were used to develop feeding standards. Derived values matched well with the actual intake versus performance of animals under diverse feeding conditions. The new standards so derived predicted requirements and intake of nutrients for different production levels better than existing feeding standards; as these are based on a more thorough analysis of a larger database, the new feeding standards will be appropriate for wide use in India.

Effects of Feeding Betaine on Performance and Blood Hormone in Laying Hens (산란계에 비태인의 급여가 생산 능력과 혈중 호르몬에 미치는 영향)

  • Park, J.H.;Kang, C.W.;Ryu, K.S.
    • Korean Journal of Poultry Science
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    • v.33 no.4
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    • pp.323-328
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    • 2006
  • An experiment was conducted to investigate the effects of dietary betaine on egg production, estradiol, progesterone, and melatonin secretion in laying hens. Seventy two ISA-Brown laying hens were placed into individual cage and fed four different levels of betaine (0, 300, 600, 1,200 ppm) in diets for four weeks. Basal diets were mainly mixed with corn-soybean and contained 2,800 kcal/kg ME and 16% CP. Dietary supplementation of betaine showed significantly higher (p<0.05) egg weight and daily eggmass than controls, but did not have consistent influence on the egg production. The serum estradiol was statistically different in betaine treatments compared to that of control (p<0.05). No significant changes in serum progesterone concentrations were observed by the addition of betaine in diets. Serum melatonin concentrations also increased with betaine supplementation in diets (p<0.05). The results of this study indicate that the dietary betaine may contribute to increase daily eggmass, serum estradiol and melatonin concentration in laying hens.

Effect of Crossbreeding of Korean Native Duck and Broiler Ducks on Performance and Carcass Yield (토종오리와 육용오리의 교배 조합이 생산성 및 도체 수율에 미치는 영향)

  • Kim, Hak-Kyu;Hong, Eui-Chul;Kang, Bo-Seok;Park, Mi-Na;Seo, Bo-Young;Choo, Hyo-Jun;Na, Seung-Hwan;Bang, Han-Tae;Seo, Ok-Suk;HwangBo, Jong
    • Korean Journal of Poultry Science
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    • v.37 no.3
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    • pp.229-235
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    • 2010
  • This work was carried out to improve the performance and the carcass yield of Korea native duck (KND) according to crossbreeding KND and broiler duck (BD). Two hundred forty out of crossbred ducks were divided into 4 strains and selected as 120 ducks separated by sex, respectively. Crossbred ducks were native ducks (female)${\times}$native ducks (male), native ducks (female)${\times}$broiler ducks (male), broiler ducks (female)${\times}$native ducks (male), and broiler ducks (female)${\times}$broiler ducks(male) as A, B, C and D, respectively. Broiler duck diet was fed as experimental diet for 0~3 weeks (CP 22.0%, ME 2,900 kcal/kg) and 3~8 weeks (CP 18.0%, ME 3,000 kcal/kg). Body weight of male was high compared to female, and that of B and C was high compared to A and low compared to D at 3 and 8 week (P<0.05). Body weight gain of male was high compared to female at 3~8 weeks and 0~8 weeks, and it was shown as D>C>B>A on body weight gain (P<0.05). In case of feed intake, there was no difference between male and female, and B and C were high compared to A and low compared to D (P<0.05). Male feed requirement was high at 3~8 weeks, and there was significant difference between A and B, C and between B and D. Carcass yield of 4 strains was shown as 70.1%, 70.8%, 73.1% and 71.2%, respectively. Finally, Crossbreeding of KND and BD may improve the performance and the carcass yield of KND.

Vitamin C enhances the expression of IL17 in a Jmjd2-dependent manner

  • Song, Mi Hye;Nair, Varun Sasidharan;Oh, Kwon Ik
    • BMB Reports
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    • v.50 no.1
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    • pp.49-54
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    • 2017
  • Previously, we reported that vitamin C facilitates the CpG demethylation of Foxp3 enhancer in $CD4^+Foxp3^+$ regulatory T cells (Tregs) by enhancing the activity of a DNA demethylase ten-eleven-translocation (Tet). However, it is not clear whether vitamin C affects other helper T cell lineages like T helper type 17 (Th17) cells which are related with Tregs. Here, we show that the expression of interleukin-17A (IL17) increases with the treatment of vitamin C but not with other antioxidants. Interestingly, the upregulation of IL17 was not accompanied by DNA demethylation in Il17 promoter and was independent of Tet enzymes. Rather, vitamin C reduced the trimethylation of histone H3 lysine 9 (H3K9me3) in the regulatory elements of the Il17 locus, and the effects of vitamin C were abrogated by knockdown of jumonji-C domain-containing protein 2 (jmjd2). These results suggest that vitamin C can affect the expression of IL17 by modulating the histone demethylase activity.

Determination of Nutritive Value of Citrus Tree Leaves for Sheep Using In vitro Gas Production Technique

  • Karabulut, Ali;Canbolat, Onder;Ozkan, Cagri O.;Kamalak, Adem
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.529-535
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    • 2007
  • The nutritive values of leaves of Citrus grandis, Citrus aurantium, Citrus oranges, Citrus limon, and Citrus deliciosa were evaluated by chemical composition and in vitro gas production techniques. There were significant (p<0.001) differences among citrus species in terms of chemical composition. Crude protein (CP) contents ranged from 123.0 to 148.3 g/kg DM. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents were varied with species in the range 219.4-355.4 and 215.0-278.8 g/kg DM respectively. Condensed tannin (CT) contents were ranged from 5.9 to 10.2 g/kg DM. The PEG addition significantly (p<0.001) increased the gas production and some estimated parameters of citrus tree leaves. However, species showed variable responses to polyethylene glycol (PEG) treatment. There were also significant (p<0.001) differences among species in terms of gas production and estimated parameters. The OMD and ME contents of citrus leaves without PEG supplementation were ranged from 66.5 to 73.3% and 9.8 to 10.9 MJ/kg DM respectively. The improvement in gas production, organic matter digestibility (OMD) and metabolizable energy (ME) with PEG emphasized the negative effect of tannins on digestibility. The increase (%) in the estimated OMD and ME contents ranged from 5.5 to 9.8% and 5.7 to 10.2% respectively. All citrus tree leaves studied in this experiment have potential nutritive values indicated by high crude protein content, OMD, ME and low fiber values.

EFFECTS OF EARLY FEED RESTRICTION ON GROWTH PERFORMANCE AND BODY COMPOSITION IN BROILERS

  • Santoso, U.;Tanaka, K.;Ohtani, S.;Youn, B.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.401-410
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    • 1993
  • Day-old male and female broilers obtained from commercial strain (Hubbard) were used in this study. At 7 days of age, all chicks were weighed individually (female 0.11 kg and male 0.12 kg in average) and divided into 5 group of 45 birds each group with no replicate in each treatment group as follows: 1. fed ad libitum; 2. fed 75% ad libitum; 3. fed 65% ad libitum; 4. fed 55% ad libitum; 5. fed 45% ad libitum. Chicks were restricted for 10 days (d 7 through 17) of a 56-day trial. Chicks were fed a commercial starter diet (crude protein (CP) 23.8% and metabolizable energy (ME) 3,070 kcal/kg) for 21 days, and commercial finisher diet (CP 20% and ME 3,160 kcal/kg) from 22 to 56 days of age. When chicks were feed-restricted at an early age, compensatory growth did not immediately occur following refeeding. Body weights of restricted chicks were not equal to the ad libitum chicks before 49 days of age. At 56 dyas of age, body weights of restricted chicks were heavier (p<0.01). Abdominal fat values of female chicks fed ad libitum was not significantly different from those fed 45% to 75% ad libitum. Carcass fat values of restricted female chicks were lower than those of control chicks when female chicks were fed either 55% or 45% ad libitum. Furthermore, feed efficiencies of restricted chicks were better. The response of chicks to early feed restriction might depend on the degree of feed restriction, and sex. Factors contributing carcass fat included energy loss, fat protein ratio of carcass, fatty acid synthesis in the liver and triglyceride content of the liver. The heavier body weights in the restricted chicks might be correlated with the lower growth rate during period of feed restriction.

Excessive Levels of Dietary Protein and Energy Induce Lack of Growth Promoting Effects of Clenbuterol in Broilers

  • Hamano, Y.;Yamazaki, S.;Kume, K.;Kobayashi, S.;Terashima, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.5
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    • pp.566-572
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    • 1998
  • The present study examined the effects of excessive dietary protein and energy on growth response to clenbuterol in broilers. The chicks were allocated into 6 groups at 14d old, and used for a $3{\times}2$ factorial experiment. Birds were fed six diets, the control diet containing 21% crude protein (CP) and 3,100 kcal of metabolizable energy ME/kg, a high protein (30% CP) or a high energy (3,500 kcal/ ME/kg) diet, with or without 1 ppm clenbuterol, for 18 d. Clenbuterol feeding markedly decreased (p < 0.05) body weight gain by 23% in the high energy group. Feed intake was also decreased (p < 0.05) by clenbuterol administration across diet treatments. Abdominal fat weight was reduced (p < 0.05) by clenbuterol only when chickens were fed the high energy diet. Clenbuterol increased (p < 0.05) leg muscle weight in the control diet group, but decreased (p < 0.05) it in the high energy group. Muscle protein concentration was increased by 11 % in leg muscle only of the birds at the high energy level. In leg muscle, clenbuterol enhanced the protein/DNA ratio by 18%, except for the high protein group. These results indicate that feeding a diet containing excessive amounts of protein and more energy than normal did not necessarily improve growth response to clenbuterol.

Comparative Rumen Degradability of Some Legume Forages between Wet and Dry Season in West Sumatra, Indonesia

  • Evitayani, Evitayani;Warly, L.;Fariani, A.;Ichinohe, T.;Abdulrazak, S.A.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.8
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    • pp.1107-1111
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    • 2004
  • An experiment was conducted to evaluate the potential nutritive value of five legume forages (leaves and twigs) in West Sumatra during wet and dry seasons. The chemical composition, in vitro dry matter (IVDMD), organic matter (IVOMD) and crude protein digestibility (IVCPD), in vitro gas characteristics and estimated metabolizable energy (ME) showed variation among legume forages and between different seasons. Crude protein (CP) ranged from 14.2 to 27.8% DM in the wet season, with a significant (p<0.05) reduction in dry season. C. pubescens, G. maculata, L. leucocephala and P. phaseloides showed the least reduction in CP content. The NDF, ADF and lignin were about 39.0, 26.5 and 6.1% DM, respectively in the wet season, and significantly (p<0.05) increased in the dry season, except for NDF of C. mucunoides and C. pubescens. For IVDMD, IVOMD and IVCPD significantly increased in the wet season, but values remained as high as over 50.0% of DM. The in vitro gas characteristics and metabolizable energy were significantly (p<0.05) higher in wet season than the dry season. During both seasons, the rate of constant (c) for G. maculata and ME content for C. pubescens and P. phaseloides were not significantly (p<0.05). Results demonstrated that P. phaseloides and L. leucocephala have a good nutritive value during both wet and dry seasons. Further studies on feeding trials are needed to quantify the animal responses when offered these legume forages.