• Genomics & Informatics
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• v.17 no.3
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• pp.27.1-27.9
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• 2019

Supernumerary B chromosomes were found in Lilium amabile (2n = 2x = 24), an endemic Korean lily that grows in the wild throughout the Korean Peninsula. The extra B chromosomes do not affect the host-plant morphology; therefore, whole transcriptome analysis was performed in 0B and 1B plants to identify differentially expressed genes. A total of 154,810 transcripts were obtained from over 10 Gbp data by de novo assembly. By mapping the raw reads to the de novo transcripts, we identified 7,852 differentially expressed genes (log₂FC > |10|), in which 4,059 and 3,794 were up-and down-regulated, respectively, in 1B plants compared to 0B plants. Functional enrichment analysis revealed that various differentially expressed genes were involved in cellular processes including the cell cycle, chromosome breakage and repair, and microtubule formation; all of which may be related to the occurrence and maintenance of B chromosomes. Our data provide insight into transcriptomic changes and evolution of plant B chromosomes and deliver an informative database for future study of B chromosome transcriptomes in the Korean lily.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.1
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• pp.41-50
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• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of Wetlands Research
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• v.18 no.4
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• pp.413-423
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• 2016

Effects of the design flood share of the Kyeongpo retarding basin, which has a function for flood control of the Kyeongpo river assigned to the Kyeongpo prickly water lily wetland, on the Kyeongpo lake and the downstream of Kyeongpo river were analyzed on the bassis of the hydraulic experiments and the numerical simulations using RMA-2 model. Reproducing a complex water flow system of the area of Kyeongpo lake, the unsteady flow simulations were performed. The data obtained in hydraulic experiments were used to determine parameters of the numerical model which simulated the flows for various flood scenarios in the downstream area of Kyeongpo river. With increasing the design flood share rates in the retarding basin, the water level was increased in the lake and is decreased in the river. The characteristics of flood flow interaction between Kyeongpo river and Kyeongpo lake were understood. These results may be used to management the Kyeongpo lake during flood season.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1169-1175
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• 2009

An antifungal chitinase, ChiCW, produced by Bacillus cereus 28-9 is effective against conidial germination of Botrytis elliptica, the causal agent of lily leaf blight. ChiCW as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-III-like domain, and a chitin-binding domain. When two C-terminal domains of ChiCW were truncated, $ChiCW{\Delta}FC$ (lacking the chitin-binding domain and fibronectin type III-like domain) lost its antifungal activity. Since $ChiCW{\Delta}C$ (lacking the chitin-binding domain) could not be expressed in Escherichia coli as $ChiCW{\Delta}FC$ did, a different strategy based on protein engineering technology was designed to investigate the involvement of the chitin-binding domain of ChiCW ($ChBD_{ChiCW}$) in antifungal activity in this study. Because ChiA1 of Bacillus circulans WL-12 is a modular enzyme with a higher hydrolytic activity than ChiCW but not inhibitory to conidial germination of Bo. elliptica and the similar domain composition of ChiA1 and ChiCW, the C-terminal truncated derivatives of ChiA1 were generated and used to construct chimeric chitinases with $ChBD_{ChiCW}$. When the chitin-binding domain of ChiA1 was replaced with $ChBD_{ChiCW}$, the chimeric chitinase named ChiAAAW exhibited both high enzyme activity and antifungal activity. The results indicate that $ChBD_{ChiCW}$ may play an important role in the antifungal activity of ChiCW.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.462-469
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• 2012

A metagenomic fosmid library was constructed from genomic DNA isolated from the microbial community residing in hindguts of a wood-feeding higher termite (Microcerotermes sp.) collected in Thailand. The library was screened for clones expressing lignocellulolytic activities. Fourteen independent active clones (2 cellulases and 12 xylanases) were obtained by functional screening at pH 10.0. Analysis of shotgun-cloning and pyrosequencing data revealed six ORFs, which shared less than 59% identity and 73% similarity of their amino acid sequences with known cellulases and xylanases. Conserved domain analysis of these ORFs revealed a cellulase belonging to the glycoside hydrolase family 5, whereas the other five xylanases showed significant identity to diverse families including families 8, 10, and 11. Interestingly, one fosmid clone was isolated carrying three contiguous xylanase genes that may comprise a xylanosome operon. The enzymes with the highest activities at alkaline pH from the initial activity screening were characterized biochemically. These enzymes showed a broad range of enzyme activities from pH 5.0 to 10.0, with pH optimal of 8.0 retaining more than 70% of their respective activities at pH 9.0. The optimal temperatures of these enzymes ranged from $50^{\circ}C$ to $55^{\circ}C$. This study provides evidence for the diversity and function of lignocellulose-degrading enzymes in the termite gut microbial community, which could be of potential use for industrial processes such as pulp biobleaching and denim biostoning.

• Genomics & Informatics
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• v.14 no.2
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• pp.53-61
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• 2016

Toxoplasma gondii is an intracellular Apicomplexan parasite and a causative agent of toxoplasmosis in human. It causes encephalitis, uveitis, chorioretinitis, and congenital infection. T. gondii invades the host cell by forming a moving junction (MJ) complex. This complex formation is initiated by intermolecular interactions between the two secretory parasitic proteins-namely, apical membrane antigen 1 (AMA1) and rhoptry neck protein 2 (RON2) and is critically essential for the host invasion process. By this study, we propose two potential leads, NSC95522 and NSC179676 that can efficiently target the AMA1 hydrophobic cleft, which is a hotspot for targeting MJ complex formation. The proposed leads are the result of an exhaustive conformational search-based virtual screen with multilevel precision scoring of the docking affinities. These two compounds surpassed all the precision levels of docking and also the stringent post docking and cumulative molecular dynamics evaluations. Moreover, the backbone flexibility of hotspot residues in the hydrophobic cleft, which has been previously reported to be essential for accommodative binding of RON2 to AMA1, was also highly perturbed by these compounds. Furthermore, binding free energy calculations of these two compounds also revealed a significant affinity to AMA1. Machine learning approaches also predicted these two compounds to possess more relevant activities. Hence, these two leads, NSC95522 and NSC179676, may prove to be potential inhibitors targeting AMA1-RON2 complex formation towards combating toxoplasmosis.

• Journal of Preventive Medicine and Public Health
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• v.54 no.1
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• pp.22-30
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• 2021

Objectives: The coronavirus disease 2019 (COVID-19) pandemic is a public health emergency posing unprecedented challenges for health authorities. Social media may serve as an effective platform to disseminate health-related information. This study aimed to assess the extent of social media use, its impact on preventive behavior, and negative health effects such as cyberchondria and information overload. Methods: A cross-sectional observational study was conducted between June 10, 2020 and August 9, 2020 among people visiting the outpatient department of the authors' institution, and participants were also recruited during field visits for an awareness drive. Questions were developed on preventive behavior, and the Short Cyberchondria Scale and instruments dealing with information overload and perceived vulnerability were used. Results: The study recruited 767 participants with a mean age of about 45 years. Most of the participants (>90%) engaged in preventive behaviors, which were influenced by the extent of information received through social media platforms (β=3.297; p<0.001) and awareness of infection when a family member tested positive (β=29.082; p<0.001) or a neighbor tested positive (β=27.964; p<0.001). The majority (63.0%) of individuals often searched for COVID-19 related news on social media platforms. The mean±standard deviation scores for cyberchondria and information overload were 9.09±4.05 and 8.69±2.56, respectively. Significant and moderately strong correlations were found between cyberchondria, information overload, and perceived vulnerability to COVID-19. Conclusions: This study provides evidence that the use of social media as an information- seeking platform altered preventive behavior. However, excessive and misleading information resulted in cyberchondria and information overload.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.26 no.1
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• pp.59-86
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• 2023

Purpose: Information regarding functional gastrointestinal disorders (FGIDs) in infants is currently lacking in Indonesia. This study aimed to describe the prevalence and risk factors of FGIDs in infants aged 6 weeks to 4 months in Indonesia. Methods: This cross-sectional study of 433 infants was conducted between September 2018 and February 2020. Information on FGIDs was collected using the Infant Gastrointestinal Symptom Questionnaire and the Feeding Practice and Gut Comfort Questionnaire. Adapted Rome IV criteria were used to define the FGIDs. Results: The prevalence of regurgitation was 26.3%; 16.8% of the infants presented cryingrelated symptoms and 5.5% exhibited constipation. The statistical analyses revealed that constipation was associated with sex (odds ratio [OR], 2.74; 95% confidence interval [CI], 1.07-7.71; p=0.043), employment of the father (OR, 0.3; 95% CI, 0.12-0.77; p=0.01), and education of the mother (OR, 1.92; 95% CI, 1.07-3.51; p=0.031). Length at birth (OR, 0.74; 95% CI, 0.55-0.99; p=0.042) was associated with constipation. Length at visit (OR, 0.83; 95% CI, 0.76-0.91; p<0.001) was associated with regurgitation, and the weight at visit (OR, 0.58; 95% CI, 0.35-0.96; p=0.038) was associated with crying and/or colic. A history of parental FGIDs was associated with crying-related symptoms (OR, 2.12; 95% CI, 1.23-3.68; p=0.007). Conclusion: Regurgitation, crying, and constipation are common FGIDs in infants. Some parental and infant characteristics may be predictors for FGIDs. Further investigations are needed to evaluate the clinical relevance of our findings. Understanding the determinants of FGIDs will benefit healthcare professionals and parents to improve infant's quality of life and better manage these condition.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.2
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• pp.105-114
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• 2018

The aim of this study was to evaluate the change of phenolic compounds after phosphite treatment on Cheorwon onion. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is widely cultivated in the temperate regions of the world. Onion is a good name for the 'Okchong' to drop blood cholesterol and cardiovascular blood flow to increase the prevention of adult diseases. Cheorwon area is inland, but it has high continental climate due to its high altitude. Therefore it is said that the onion cultivated in this region has higher sugar content and higher taste than onion grown in the southern region. Phosphorus components are particularly important ingredients for promoting muscle development. However, if the phosphoric acid content of the soil part is maintained to a large extent until the harvest, the competition of the nutrients tends to cause decay of the root part. Therefore, it is important to improve the quality and shelf life of onion by inducing nutrient balance by applying foliar fertilization method on the reducing phosphorus at harvest time. In this study, acidity was controlled by diluting phosphorous acid(H₃PO₃) and potassium hydroxide(KOH), followed by leaf surface treatment with phosphite on onion. In this study, the concentration of phosphite was diluted to 500, 1,000, 1,500ppm and sprayed three times over the onion leaves in May 2018 using an atomizer and harvested at the end of June, and the phenolic compounds were analyzed by HPLC. As a result, the content of quercetin, one of the important substances in onion, was phosphite 500ppm(179.70㎍/g), 1,000(150.27), 1,500(105.95). The contents of caffeic acid, p-coumaric acid, ferulic acid, rutin, kaempferol, and sugar content were higher in the treatments than in the control. Therefore, the phosphite does not have a great influence on the growth, but it may play a role as a method of achieving balance with nitrogen in the rainy season by supplying the role of the material catalyst and the water soluble phosphoric acid and the potassium in the influence of the material change.

• The Korean Journal of Pesticide Science
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• v.5 no.1
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• pp.61-67
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• 2001

Five hundred sixteen isolates of Botrytis elliptica were isolated from infected leaves of Lilium longiflorum from Kangwon alpine areas in Korea during tile seasons from 1998 to 2000 and resistance of these isolates against some fungicides were examined. The isolation frequency of phenotypes resistant to benomyl, procymidone, and diethofencarb were 90.1, 32.4, and 40.9%, respectively. The isolates were divided into six phenotypic groups; RSS, RRS, SSR, SRR, RSR and RRR, representing sensitive (S) or resistant (R) to benzimidazole, dicarboximide, and N-phenylcarbamate fungicides in order. The percentage of six phenotypes were 40.7, 8.5, 7.2, 2.7, 19.8, and 21.1%, respectively. The RSS phenotype was the most frequently isolated, and tile SRR consisted of the extremely minor populations. In comparison studies on tile overwintering ability of each phenotype in relation to the others, the most frequently isolated RSS and SSR had the higher fitness ability than the less frequently isolated RSR, SRR, and RRR. Recently, population increase of tile RSR and RRR phenotypes may have resulted from the increased applications of the mixture of carbendazim and diethofencarb to control benzimidazole-resistant B. elliptica since 1998. The results of this study indicate that careful application of the fungicides is necessary to achieve effective control of leaf blight on lily in Korea.