• Title/Summary/Keyword: Maximum growth rate

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Effects of Temperature and Salinity on the Growth and Paralytic Shellfish Toxin (PST) Production by Toxic Dinoflagellate Alexandrium pacificum (유독 와편모조류 Alexandrium pacificum의 생장과 마비성 패독 생산에 미치는 수온과 염분의 영향)

  • Li, PeiJin;Oh, Seok Jin;Kim, Seok-Yun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.6
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    • pp.866-873
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    • 2022
  • Growth rate and production of the paralytic shellfish poisoning toxin (PST) of a toxic dinoflagellate Alexandrium pacificum (LIMS-PS-2611) isolated from the southern sea of Korea, were examined under various temperatures and salinity conditions. The maximum growth rate (0.28 day-1) was observed under 25℃ and 30 psu. Optimal growth (≥ 70% of maximum growth rate) was obtained between 20~25℃ and 25~35 psu. Among the PSTs of A. pacificum, the principal toxins were C1+2 and GTX5 in N-sulfocarbamoyl toxin group, and minor components were characterized as neoSTXs in the carbamate toxin group. Maximum toxin content was observed under 20℃ and 30 psu, and the toxin content increased with the increase of salinity. Low toxin contents were measured under the temperature and salinity conditions of the maximum growth rate. Therefore, the PSP of bivalve, which occurs at a temperature range of 20-25℃ in June, might have been derived from A. pacificum.

Optimization of Betacyanin Production by Red Beet (Beta vulgaris L.) Hairy Root Cultures. (Red Beet의 모상근 배양을 이용한 천연색소인 Betacyanin 생산의 최적화)

  • Kim, Sun-Hee;Kim, Sung-Hoon;Lee, Jo-No;An, Sang-Wook;Kim, Kwang-Soo;Hwnag, Baik;Lee, Hyeong-Yong
    • Microbiology and Biotechnology Letters
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    • v.26 no.5
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    • pp.435-441
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    • 1998
  • Optimal conditions for the production of natural color, betacyanin were investigated by varying light intensity, C/N ratio, concentrations of phosphate and kinds of elicitors. Batch cultivation was employed to characterize cell growth and betacyanin production of 32 days. The maximum specific growth rate, ${\mu}$$\sub$max/, was 0.3 (1/day) for batch cultivation. The maximum specific production rate, q$\^$max/$\sub$p/, was enhanced 0.11 (mg/g-cell/day) at 3 klux. A light intensity of 3 klux was shown to the best for both cell growth and betacyanin production. The maximum specific production rate was 0.125 (mg/g-cell/day) at 0.242 (1/day), the maximum specific growth rate. The dependence of specific growth rate on the light lintensity is fit to the photoinhibition model. The correlation between ${\mu}$ and q$\sub$p/ showed that the product formation parameters, ${\alpha}$ and ${\beta}$$\sub$p/ were 0.3756 (mg/cell) and 0.001 (mg/g-cell/day), respectively. The betacyanin production was partially cell growth related process, which is different from the production of a typical product in plant cell cultures. In C/N ratio experiment, high carbon concentration, 42.1 (w/w) improved cell growth rate while lower concentration, 31.6 (w/w) increased the betacyanin production rate. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.26 (1/day) and 0.075 (mg/g-cell/day), respectively. Beta vulgaris L. cells under 1.25 mM phosphate concentration produced 10.15 mg/L betacyanin with 13.46 (g-dry wt./L) of maximum cell density. The production of betacyanin was elongated by adding 0.1 ${\mu}$M of kinetin. This also increased the cell growth. Optimum culture conditions of light intensity, C/N, phosphate concentration were obtained as 5.5 klux, 27 (w/w), 1.25 mM, respectively by the response surface methodology. The maximum cell density, X$\sub$max/, and maximum production, P$\sub$max/, in optimized conditions were 16 (g-dry wt./L), 12.5 (mg/L) which were higher than 8 (g-dry wt./L), 4.48 (mg/L) in normal conditions. The ${\mu}$$\sub$max/ and q$\^$max/$\sub$p/ were 0.376 (1/day) and 0.134 (mg/g-cell/day) at the optimal condition. The overall results may be useful in scaling up hairy root cell culture system for commercial production of betacyanin.

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Effects of Gas Flow Variables on the Crystal Growth of Diamond in Hot Filament-Assisted CVD (고온 필라멘트 다이아몬드 CVD에서 기체유동변수가 결정성장에 미치는 영향)

  • 서문규;이지화
    • Journal of the Korean Ceramic Society
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    • v.31 no.1
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    • pp.88-96
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    • 1994
  • Hot filament-assisted CVD was carried out to deposit diamond films on Si(100) substrate at 90$0^{\circ}C$ using a 1% CH4-H2 mixture gas. Deposition was made at various conditions of mass flow rate of the feed gas (30~1000 sccm), pressure (2.5~300 Torr), and filament-substrate distance (4~15 mm), and the deposited films were characterized by SEM, XRD, and Raman spectroscopy. As the flow rate increases, the growth rate also increased but the crystallinity of the film was degraded. A longer filament-substrate distance simply caused both the growth rate and the crystallinity to become poorer. On the other hand, the pressure variation resulted in a maximum growth rate of 2.6 ${\mu}{\textrm}{m}$/hr at 10 Torr and the best film quality around 50 Torr, exhibiting an optimum condition. The observed trends were interpreted in terms of the flow velocity-dependent pyrolysis reaction efficiency and mass transport through the boundary layer.

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Photosynthesis and Growth Responses of Soybean (Glycine max Merr.) under Elevated CO2 Conditions (대기 중 CO2 상승 조건에서 재배되는 콩의 광합성과 생장 반응의 분석)

  • Oh, Soonja;Koh, Seok Chan
    • Journal of Environmental Science International
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    • v.26 no.5
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    • pp.601-608
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    • 2017
  • The effects of elevated atmospheric $CO_2$ on growth and photosynthesis of soybean (Glycine max Merr.) were investigated to predict its productivity under elevated $CO_2$ levels in the future. Soybean grown for 6 weeks showed significant increase in vegetative growth, based on plant height, leaf characteristics (area, length, and width), and the SPAD-502 chlorophyll meter value (SPAD value) under elevated $CO_2$ conditions ($800{\mu}mol/mol$) compared to ambient $CO_2$ conditions ($400{\mu}mol/mol$). Under elevated $CO_2$ conditions, the photosynthetic rate (A) increased although photosystem II (PS II) photochemical activity ($F_v/F_m$) decreased. The maximum photosynthetic rate ($A_{max}$) was higher under elevated $CO_2$ conditions than under ambient $CO_2$ conditions, whereas the maximum electron transport rate ($J_{max}$) was lower under elevated $CO_2$ conditions compared to ambient $CO_2$ conditions. The optimal temperature for photosynthesis shifted significantly by approximately $3^{\circ}C$ under the elevated $CO_2$ conditions. With the increase in temperature, the photosynthetic rate increased below the optimal temperature (approximately $30^{\circ}C$) and decreased above the optimal temperature, whereas the dark respiration rate ($R_d$) increased continuously regardless of the optimal temperature. The difference in photosynthetic rate between ambient and elevated $CO_2$ conditions was greatest near the optimal temperature. These results indicate that future increases in $CO_2$ will increase productivity by increasing the photosynthetic rate, although it may cause damage to the PS II reaction center as suggested by decreases in $F_v/F_m$, in soybean.

Study of Matter Production and Phothsynthetic Characteristics in Wild Vegetable(Chwinamul) (취나물류의 물질생산 및 광합성 특성 I. 취나물류의 포장재배시 차광 및 시비조건에 따른 광합성속도의 차이)

  • 조동하
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.86-92
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    • 1998
  • The photosynthetic rate (LPS)in the field was higher in the non-shading condition than in the shading. The fertilizer application was somewhat higher than non-fertilizer application. After humus application at 50% sading condition the growth and LPS increased with Ligularia fischeri. The maximum LPS at 80% shading was 22.3${\mu}{\textrm}{m}$mol/m/s with N-fertilizer application . Except Aster tataricus and Solidago virge-aurea. var. asiatic where the maximum LPS at non-shading and N-fertilizer application were 38.68 and 35.28${\mu}{\textrm}{m}$mol/m2/s. While the maximum LPS of Aster scaber was 30.01${\mu}{\textrm}{m}$mol/$m^2$/s at non-shading and non-fertilizer application. the growth rate was higher shading and fertilized conditions than non-shading and non-fertilized . The most effective shading condition was 50% , but leaf ration was highest in the 80% . But a. tataricus was favorable at non-shading and N-feritlized conditions.

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Effect of Fermentation Temperature on the Production of high content Alcohol (고농도 알코올 생성을 위한 온도의 영향)

  • 유연우;권정주
    • Korean Journal of Microbiology
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    • v.20 no.2
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    • pp.67-72
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    • 1982
  • The effect of fermentation temperature on the production of high content alcohol has been investigated with high substrate concentration. The maximum specific growth rate, ${\mu}max\;was\;0.461hr^{-1}\;at\;35^{\circ}C$ which was the highest, whereas the maximum biomass concentration waas 8.7g/l at $25^{\circ}C$, at the growth rate lower than at $35^{\circ}C$. Approximately 140g/l of ethanol was produced in the temperature range of 20 to $25^{\circ}C$ with nearly complete comsumption of the substrate. Extended fermentation time has been required at lower temperatures, however, for the maximum values of biomass concentration and alcohol content, hence higher ethanol productivity, as the temperature was elevated to $40^{\circ}C$. The viability of yeasts was greatly improved by lowering the fermentation temperature down to $25^{\circ}C$ and also extended survival of the cells has been observed at lower fermentation temperatures, although the ethanol concentration of both waas higher.

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Metalorganic Chemical Vapor Deposition of Aluminum Thin Film for ULSI Using Dimethylethylamine Alane(DMEAA) (DMEAA를 이용한 초고집적 회로용 알루미늄 박막의 제조)

  • 이기호;김병엽;이시우
    • Journal of the Korean Vacuum Society
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    • v.4 no.S1
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    • pp.81-86
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    • 1995
  • Aluminum has been deposited selectively on TiN surfaces in the presence of Si, SiO2 from Dimethyethylamine Alane(DMEAA). The film properties of the deopsited AI film were determined by various methods(SEM, Auger, UV-photospectrometer, Four point-probe, XRD). The effect of in-situ H2 plasma precleaning was studied. The effect of gap distance, pressure and temperature on the properties(crystallinity, resistance, grain size, morphology) of AI film and on the growth rates was investigated. It was found that the plasma precleaning promotes the growth rate and there exists optimum thmperature for maximum growth rate.

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Abnormal Behavior of Ordinary Heterotrophic Organism Active Biomass at Different Substrate/Microorganisms Ratios in Batch Test (회분식 실험 Substrate/Microorganisms 비에 따른 종속영양미생물의 특이거동 연구)

  • Lee, Byung-Joon;Wentzel, M.C.;Ekama, G.A.;Min, Kyung-Sok
    • Journal of Korean Society on Water Environment
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    • v.20 no.3
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    • pp.197-205
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    • 2004
  • Batch test methods have developed for a long time to measure kinetic and stoichiometric parameters which are required to perform steady state design and mathematical modelling of activated sludge processes. However, at various So/Xo ratios, abnormal behaviors of ordinary heterotrophic organism in batch tests have been reported in many researches. Thus, in this research, abnormal behaviors of heterotrophs in batch tests were investigated at various So/Xo conditions by measuring and interpreting oxygen utilization rate. As So/Xo ratio increased, the calculated values of maximum specific growth rates, ${\mu}_{H,max}$ and $K_{MP,max}$, increased. However, at a certain point of So/Xo (around 10mgCOD/mgMLAVSS), ${\mu}_{H,max}$ and $K_{MP,max}$ values started to decrease. According to this observation, three prominent behaviours of heterotrophs were identified at various So/Xo conditions. (1) At low So/Xo region (below 5 mgCOD/mgMLAVSS), the oxygen utilization rate of heterotrophs in batch tests were almost stable and consequently yielded lower maximum specific growth rate. (2) At high So/Xo region (up to 5~10 mgCOD/mgMLAVSS), oxygen utilization rate incresed sharply with time and indicated more upward curvature than the predicted OUR with conventional activated sludge model, which consists of single hetetrotrophs group. Thus, in this region, competition model of two organisms, fast-grower and slow-grower, seemed to be appropriate. (3) At extremely high So/Xo region (over 10mgCOD/mgMLAVSS), significant oxygen utilization rate was still observed even after depletion of readily biodegradable COD. This might be caused by retarded utilization of intermediates which were generated by self inhibition mechanism in the process of RBCOD uptake.

Flow Characteristics of a Laminar Rivulet Down an Inclined Surface (경사면상의 층류 세류유동 특성)

  • Kim, Byong-Joo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.17 no.11
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    • pp.1035-1042
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    • 2005
  • In the present study, the principle of minimum energy is employed to configure the shape of rivulet flowing down an inclined surface. The profile of laminar rivulet is determined by numerical integration. The maximum center thickness, which corresponds to the minimum thickness of falling film, is found to exist regardless of liquid flow rate and is compared with the analytical and experimental data. At small liquid flow rate the center thickness of rivulet and its width increase almost linearly with flow rate. Once the center thickness of rivulet becomes very close to its maximum value, its growth rate retards abruptly. However the width of rivulet increases proportionally to the liquid flow rate and most part of its free surface is as flat as that of stable film. The growth rate of rivulet thickness with respect to liquid flow rate becomes larger at bigger contact angle. The width of rivulet increases rapidly with its flow rate especially at small contact angle, As the liquid-vapor interfacial shear stress increases, the center thickness of rivulet decreases with its flow rate, which is remarkable at small contact angle. However the effect of interfacial shear stress on the width of rivulet is almost negligible.

Essence of thermal convection for physical vapor transport of mercurous chloride in regions of high vapor pressures

  • Kim, Geug-Tae;Lee, Kyong-Hwan;Choi, Jeong-Gil
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.17 no.6
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    • pp.231-237
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    • 2007
  • For an aspect ratio (transport length-to-width) of 5, Pr=3.34, Le=0.078, Pe=4.16, Cv=1.01, $P_B=50$ Torr, only thermally buoyancy-driven convection ($Gr=4.83{\times}10^5$) is considered in this study in spite of the disparity in the molecular weights of the component A ($Hg_2Cl_2$) and B which would cause thermally and/or solutally buoyancy-driven convection. The crystal growth rate and the maximum velocity vector magnitude are decreased exponentially for $3{\le}Ar{\le}5$, for (1) adiabatic walls and (2) the linear temperature profile, with a fixed source temperature. This is related to the finding that the effects of side walls tend to stabilize convection in the growth reactor. The rate for the linear temperature profiles walls is slightly greater than for the adiabatic walls far varied temperature differences and aspect ratios. With the imposed thermal profile, a fixed source region, both the rate and the maximum velocity vector magnitude increase linearly with increasing the temperature difference for $10{\le}{\Delta}T{\le}50K$.