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http://dx.doi.org/10.7837/kosomes.2022.28.6.866

Effects of Temperature and Salinity on the Growth and Paralytic Shellfish Toxin (PST) Production by Toxic Dinoflagellate Alexandrium pacificum  

Li, PeiJin (Major of Oceanography, Division of Earth and Environmental System Sciences, Pukyong National University)
Oh, Seok Jin (Major of Oceanography, Division of Earth and Environmental System Sciences, Pukyong National University)
Kim, Seok-Yun (Major of Oceanography, Division of Earth and Environmental System Sciences, Pukyong National University)
Publication Information
Journal of the Korean Society of Marine Environment & Safety / v.28, no.6, 2022 , pp. 866-873 More about this Journal
Abstract
Growth rate and production of the paralytic shellfish poisoning toxin (PST) of a toxic dinoflagellate Alexandrium pacificum (LIMS-PS-2611) isolated from the southern sea of Korea, were examined under various temperatures and salinity conditions. The maximum growth rate (0.28 day-1) was observed under 25℃ and 30 psu. Optimal growth (≥ 70% of maximum growth rate) was obtained between 20~25℃ and 25~35 psu. Among the PSTs of A. pacificum, the principal toxins were C1+2 and GTX5 in N-sulfocarbamoyl toxin group, and minor components were characterized as neoSTXs in the carbamate toxin group. Maximum toxin content was observed under 20℃ and 30 psu, and the toxin content increased with the increase of salinity. Low toxin contents were measured under the temperature and salinity conditions of the maximum growth rate. Therefore, the PSP of bivalve, which occurs at a temperature range of 20-25℃ in June, might have been derived from A. pacificum.
Keywords
Alexandrium pacificum; Growth rate; Paralytic shellfish toxin; Temperature; Salinity;
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1 Oikawa H., T. Fujita, K. Satio, S. Watabe, M. Satomi, and Y. Yano(2004), Comparison of paralytic shellfish poisoning toxin between carnivorous crabs (Telmessus acutidens and Charybdis japonica) and their prey mussel (Mytilus galloprovincialis) in an inshore food chain, Toxicon, Vol. 43, No. 6, pp. 713-719.   DOI
2 Wang, D. Z. and D. P. H. Hsieh(2005), Growth and toxin production in batch cultures of a marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea, Harmful Algae, Vol. 4, No. 2, pp. 401-410.   DOI
3 Xu, J., A. Y. T. Ho, L. He, K. Yin, C. Hung, N. Choi, P. K. S. Lam, R. S. S. Wud, D. M. Anderson, and P. J. Harrison(2012), Effects of inorganic and organic nitrogen and phosphorus on the growth and toxicity of two Alexandrium species from Hong Kong, Harmful Algae, Vol. 16, pp. 89-97.   DOI
4 Yoshida, T., Y. Sako, and A. Uchida(2001), Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese populations of Alexandrium tamarense and A. catenella (Dinophyceae), Phycological Research, Vol. 49, No. 1, pp. 13-21.   DOI
5 Larocque, R. and A. D. Cembella(1990), Ecological parameters associated with the seasonal occurrence of Alexandrium spp. and consequent shellfish toxicity in the lower St. Lawrence estuary (Eastern Canada). In Graneli, E., B. Sundstrom, L. Edler, and D. M. Anderson (Eds.), Toxic Marine Phytoplankton, Elsevier, Amsterdam, pp. 368-373.
6 Maclntyre, J. G., J. J. Cullen, and A. D. Cembella(1997), Vertical migration, nutrition and toxicity in the dinoflagellate Alexandrium tamarense, Marine Ecology and Progress Series, Vol. 148, pp. 201-216.   DOI
7 Shon, M. B., Y. S. Kim, and C. H. Kim(2009), Paralytic shellfish poisoning of Mediterranean mussels from Jinhae Bay in Korea, Korean Journal Fisheries and Aquatic Sciences, Vol. 42, No. 4, pp. 366-372.   DOI
8 Maranda, L., D. M. Anderson, and Y. Shimizu(1985), Comparison of toxicity between populations of Gonyaulax tamarensis of eastern North American waters, Estuarine, Coastal and Shelf Science, Vol. 21, No. 3, pp. 401-410.   DOI
9 Anderson, D. M., D. M. Kulis, J. J. Sullivan, S. Hall, and C. Lee(1990), Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp, Marine Biology, Vol. 104, pp. 511-524.   DOI
10 Oh S. J., J. -A. Park, H. -K. Kwon, H. S. Yang, and W. -A. Lim(2012), Ecophysiological studies on the population dynamics of two toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella isolated from the southern coast of Korea I. Effects of temperature and salinity on the growth, Journal of the Korean Society for Marine Environmental Engineering, Vol. 15, No. 2, pp. 133-141.   DOI
11 Siu, G. K. Y., M. L. C. Young, and D. K. O. Chan(1997), Environmental and nutritional factors which regulate population dynamics and toxin production in the dinoflagellate Alexandrium catenella, Hydrobiologia, Vol. 352, pp. 117-140.   DOI
12 Sunda, W. G., E. Graneli, and C. J. Gobler(2006), Positive feedback and the development and persistence of ecosystem disruptive algal blooms, Journal of Phycology, Vol. 42, No. 5, pp. 963-974.   DOI
13 Tanabe, S. H. and Y. Sako(2006), Genetic differentiation in the marine dinoflagellates Alexandrium tamarense and Alexandrium catenella based on DNA-DNA hybridization, Plankton and Benthos Research, Vol. 1 (3), pp. 138-146.   DOI
14 Taroncher-Oldenburg, G., D. M. Kulis, and D. M. Anderson (1997), Toxin variability during the cell cycle of the dinoflagellate Alexandrium fundyense, Limnology and Oceanography, Vol. 42, No. 5, pp. 1178-1188.   DOI
15 Sekiguchi, K., S. Sato, S. Kaga, T. Ogata, and M. Kodama (2001), Accumulation of paralytic shellfish poisoning toxins in bivalves and an ascidian fed on Alexandrium tamarense cells, Fisheries Sciense, Vol. 67, No. 2, pp. 301-305.   DOI
16 Shin, H. H., Z. Li, E. S. Kim, J-W. Park, and W. A. Lim(2017), Which species, Alexandrium catenella (Group I) or A. pacificum (Group IV), is really responsible for past paralytic shellfish poisoning outbreaks in Jinhae-Masan Bay, Korea?, Harmful Algae, Vol. 68, pp. 31-39.   DOI
17 Boyer, G. L., J. J. Sullivan, R. J, Andersen, P. J. Harrison, and F. J. R. Taylor(1987), Effects of nutrient limitation on toxin production and composition in the marine dinoflagellate Protogonyaulax tamarensis, Marine Biology, Vol. 96, pp. 123-128.   DOI
18 Anderson, D. M.(1990), Toxin variability in Alexandrium species. In: Graneli, E., B. Sundstrom, L. Edler, and D. M. Anderson (Eds.), Toxic Marine Phytoplankton, Elsevier, Amsterdam, pp. 41-51.
19 Hamasaki, K., M. Horie, S. Tokimistu, T. Toda, and S. Taguchi(2001), Variability in toxicity of the dinoflagellate Alexandrium tamarense isolated from Hiroshima Bay, western Japan, as a reflection of changing environmental conditions, Journal of Plankton Research, Vol. 23, No. 3, pp. 271-278.   DOI
20 Boczar, B. A., M. K. Beitler, J. Liston, J. Sullivan, and R. A. Cattolico(1988), Paralytic shellfish toxins in Protogonyaulax tamarensis and Protogonyaulax catenella in axenic culture, Plant Physiology, Vol. 88, No. 4, pp. 1285-1290.   DOI
21 Caruana, A. M. N., M. Le Gac, F. Herve, G.-A. Rovillon, S. Geffroy, F. Malo, E. Abadie, and Z. Amzil(2020), Alexandrium pacificum and Alexandrium minutum: harmful or environmentally friendly?, Marine Environmental Research, Vol. 160, pp. 105014.   DOI
22 Franks, P. J. S. and D. M. Anderson(1992), Alongshore transport of a toxic phytoplankton bloom in a buoyancy current: Alexandrium tamarense in the Gulf of Maine, Marine Biology, Vol. 112, pp. 153-164.   DOI
23 Hadjadji, I., M. Laabir, H. Frihi, Y. Collos, Z. J. Shao, P. Berrebi, E. Abadie, Z. Amzil, N. Chomerat, J. L. Rolland, F. Rieuvilleneuve, and E. Masseret(2020), Unsuspected intraspecific variability in the toxin production, growth and morphology of the dinoflagellate Alexandrium pacificum R. W. Litaker (Group IV) blooming in a South Western Mediterranean marine ecosystem, Annaba Bay (Algeria), Toxicon, Vol. 180, pp. 79-88.   DOI
24 Taroncher-Oldenburg, G., D. M. Kulis, and D. M. Anderson (1999), Coupling of saxitoxin biosynthesis to the G1 phase of the cell cyclc in the dinoflagellate Alexandrium fundyense: temperature and nutrients effects, Nat Toxins, Vol. 7 (5), pp. 207-219.   DOI
25 Taylor, F. J. R., Y. Fukuyo, and J. Larsen(1995), Taxonomy of harmful dinoflagellates. In: Hallegreaff, M., D. M., Anderson, A. D. Cembella and H. O. Enevoldsen (Eds), Manual on Harmful Marine Microalgae, UNESCO, Paris, pp. 283-317.
26 Fulco, V. K. and A. M. Gayoso(2004), Effects of light, temperature and salinity on the growth rate of Alexandrium tamarense from Patagonia (Argentina). In Steidinger, K. A., J. H. Landsberg, C. R. Tomas and G. A. Vargo (Eds), Harmful Algae 2002, Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and Intergovernmental Oceanographic Commission of UNESCO, Florida, pp. 391-392.
27 Goldman, J. C. and E. J. Carpenter(1974), A kinetic approach to the effect of temperature on algal growth, Limnology and Oceanography, Vol. 19, No. 5, pp. 756-766.   DOI
28 Guillard, R. R L. and J. H. Ryther(1962), Studies of marine planktonic diatoms. I. Cyclotellanana Hustedt and Detonula confervacea (Cleve) Gran, Canadian Journal of Microbiology, Vol. 8, No. 2, pp. 229-239.   DOI
29 Hallegraeff, G. M.(1993), A review of harmful algal blooms and their apparent global increase. Phycologia, Vol. 32(2), pp. 79-99.   DOI
30 Hwang, D. F. and Y. H. Lu(2000), Influence of environmental and nutritional factors on growth, toxicity, and toxin profile of dinoflagellate Alexandrium minutum, Toxicon, Vol. 38, No. 11, pp. 1491-1503.   DOI
31 Matsuda, A., T. Nishijima, K. Fukami, and M. Adachi(2006), Growth kinetics and paralytic shellfish poisoning toxin production in phosphorus limited cultures of Alexandrium catenella, Nippon Suisan Gakkaishi, Vol. 72, No. 2, pp. 193-200.   DOI
32 Hallegraeff, G. M.(2010), Ocean climate change, phytoplankton community responses, and harmful algal blooms: A formidable predictive challenge, Journal of Phcology, Vol. 46, pp. 220-235.   DOI
33 Anderson, D. M., T. J. Alpermann, A. D. Cembella, Y. Collos, E. Masseret, and M. Montreson(2012), The globally distributed genus Alexandrium: Multifaceted roles in marine ecosystems and impacts on human health, Harmful Algae, Vol. 14, pp. 10-35   DOI
34 Cembella, A. D., J. J. Sullivan, G. L. Boyer, F. J. R. Taylor, and R. J. Anderson(1987), Variation in paralytic shellfish toxin composition within the Protogonyaulax tamarensis/catenella species complex: red tide dinoflagellates, Biochemical Systematics and Ecology, Vol. 15, No. 2, pp. 171-186.   DOI
35 Parkhill, J. P. and A. D. Cembella(1999), Effects of salinity, light and inorganic nitrogen on growth and toxigenicity of the marine dinoflagellate Alexandrium tamarense from northeastern Canada, Journal of Plankton Research, Vol. 21 No. 5, pp. 939-955.   DOI
36 Ishida, Y., C. -H. Kim, Y. Sako, N. Hirooka, and A. Uchida(1993), PSP toxin production is chromosome dependent in Alexandrium spp. In: Smayda, T. J. and Y. Shimizu (Eds.), Toxic Phytoplankton Blooms in the Sea, Elsevier, Netherlands, pp. 881-887.
37 Kim, K. Y., M. Yoshida, Y. Fukuyo, and C. H. Kim(2002), Morphological Observation of Alexandrium tanarense (Lebour) Balech, A. catenella (Whedon et Kofoid) Balech and one related morphotype (Dinophyceae) in Korea, Algae, Vol. 17, No. 1, pp. 11-19.   DOI
38 Laabir, M., Y. Collos, E. Masseret, V. Savart, M. Sibat, and Z. Amzil(2013), Influence of environmental factors on the paralytic shellfish toxin content and profile of Alexandrium catenella (Dinophyceae) isolated from the Mediterranean Sea, Marine Drugs, Vol. 11, No. 5, pp. 1583-1601.   DOI
39 Kremp, A., A. Godhe, J. Egardt, S. Dupont, S. Suikkanen, S. Casabianca, and A. Penna(2012), Intraspecific variability in the response of bloom-forming marine microalgae to changed climate conditions, Ecology and Evolution, Vol. 2, No. 6, pp. 1195-1207.   DOI
40 Lim, P. -T. and T. Ogata(2005), Salinity effect on growth and toxin production of four tropical Alexandrium species (Dinophyceae), Vol. 45, No. 6, pp. 699-710.
41 Mok, J. -S., K. -C. Song, K. -J. Lee, and J. -H. Kim(2013), Variation and profile of paralytic shellfish poisoning toxins in Jinhae Bay, Korea, Fisheries and Aquatic Sciences, Vol. 16, No. 3, pp. 137-142.   DOI
42 NIFS(2022), https://www.nifs.go.kr/bbs?id=shellfish
43 Ogata, T., T. Ishimaru, and M. Kodama(1987), Effect of water temperature and light intensity on growth rate and toxicity change in Protogonyaulax tamarensis, Marine Biology, Vol. 95, pp. 217-220.   DOI
44 Oshima, Y.(1995), Post-column derivatization HPLC methods of paralytic shellfish poisons, In; Manual on harmful marine microalgae, Hallegraeff, G. M., D. M. Anderson and A. D. Cembella(eds), UNESCO, Paris, pp. 81-94.
45 Oshima, Y., S. I. Blackburn, and G. M. Hallegraeff(1993), Comparative study on paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum from three different countries, Marine Biology, Vol. 116, pp. 471-476.   DOI