• Parasites, Hosts and Diseases
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• 제59권4호
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• pp.403-408
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• 2021

Avian coccidiosis has a major economic impact on the poultry industry, it is caused by 7 species of Eimeria, and has been primarily controlled using chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. We assessed anticoccidial effects of berberine-based diets in broiler chickens following oral infection with 5 Eimeria species (E. acervulina, E. maxima, E. tenella, E. mitis, and E. praecox). When 0.2% berberine, a concentration that does not affect weight gain, was added to the diet, the 4 groups infected with E. acervulina, E. tenella, E. mitis, or E. praecox showed significant reductions in fecal oocyst shedding (P<0.05) compared to their respective infected and untreated controls. In chickens treated 0.5% berberine instead of 0.2% and infected with E. maxima, fecal oocyst production was significantly reduced, but body weight deceased, indicating that berberine treatment was not useful for E. maxima infection. Taken together, these results illustrate the applicability of berberine for prophylactic use to control most Eimeria infections except E. maxima. Further studies on the mechanisms underlying the differences in anticoccidial susceptibility to berberine, particularly E. maxima, are remained.

• ALGAE
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• 제37권3호
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• pp.239-247
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• 2022

Enzyme-assisted hydrolysis is frequently used as a cost-effective and efficient method to obtain functional ingredients from bioresources. This study involved the enzyme-assisted hydrolyzation and purification of fucoidan from Ecklonia maxima stipe and the investigation of its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Fucoidans of Viscozyme-assisted hydrolysate from E. maxima (EMSFs) harvested in Jeju, Korea. Structural and chemical characterizations were performed using fourier transform infrared spectroscopy, scanning electron microscope, and monosaccharide analysis. Among fucoidans, EMSF6 was rich in fucose and sulfate and had a similar structural character to commercial fucoidan. EMSF6 showed a strong inhibitory effect on nitric oxide generation in LPS-induced RAW 264.7 cells and significantly decreased the production of LPS-induced pro-inflammatory cytokines, including interleukin-6, interleukin-1β, and tumor necrosis factor α. The anti-inflammatory potential of EMSF6 was mediated through the downregulation of inducible nitric oxide synthase and cyclooxygenase-2 expression. Thus, fucoidans from E. maxima stipe are promising candidates for functional food products.

• Wind and Structures
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• 제9권3호
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• pp.179-191
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• 2006

Parent wind data are often acknowledged to fit a Weibull probability distribution, implying that wind epoch maxima should fall into the domain of attraction of the Gumbel (Type I) extreme value distribution. However, observations of wind epoch maxima are not fitted well by this distribution and a Generalised Extreme Value (GEV) analysis leading to a Type III fit empirically appears to be better. Thus there is an apparent paradox. The reasons why advocates of the GEV approach seem to prefer it are briefly summarised. This paper gives a detailed analysis of the errors involved when the GEV is fitted to epoch maxima of Weibull origin. It is shown that the results in terms of the shape parameter are an artefact of these errors. The errors are unavoidable with the present sample sizes. If proper significance tests are applied, then the null hypothesis of a Type I fit, as predicted by theory, will almost always be retained. The GEV leads to an unacceptable ambiguity in defining design loads. For these reasons, it is concluded that the GEV approach does not seem to be a sensible option.

• Journal of Plant Biology
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• 제37권4호
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• pp.453-458
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• 1994

울릉도산 홍조 산호말과 식물 4종, Amphiroa beauvoisii Lamouroux, Jania yenoshimensis (Yendo) Silva 및 Corallina officinalis Linnaeus에 대한 분류학적 검토를 수행하였다. S. maxima (Yendo) Silva는 본 연구를 통하여 처음으로 보고되는 한국산 미기록 식물이다. J. yenoshimensis (Yendo) Yendo는 형태적 특징이 기준표본의 기재와 잘 일치하였고, 외형적 특성에서 J. arborescens와 다소 유사하지만 식물체의 크기와 분지양상에서 뚜렷이 구분되었다. C. officinalis는 다수의 수세포층을 갖는다는 점을 제외하고 유럽산 식물과 형태적 특징이 일치하였다.

• 한국산학기술학회논문지
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• 제10권2호
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• pp.358-363
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• 2009

자연이미지로부터 텍스트 영역 검출은 다양한 응용분야에 활용됨으로 이 분야의 많은 연구가 필요하다. 최근의 연구 방법은 에지 및 연결요소 기반 방법을 결합하는 다양한 알고리즘을 이용하여 텍스트 영역을 검출하고 있다. 그러므로 본 논문은 이러한 결합방법으로 에지 및 국부적 최소/최대 변환 방법을 이용하여 텍스트 영역을 검출하는 알고리즘을 제안한다. 명도 이미지로부터 에지 및 국부적 최소/최대 연결성분을 검출하고, 에지 및 국부적 최소/최대 연결성분을 레이블화한다. 레이블된 영역을 분석하여 텍스트 후보 영역을 검출하고, 검출된 각각의 텍스트 후보 영역을 결합하여 단일 텍스트 후보 이미지를 생성한다. 텍스트 후보 개별문자의 인접성 및 유사도를 비교하여 검증함으로서 최종적인 텍스트 영역을 검출한다. 실험결과 제안한 알고리즘은 에지 요소 및 국부적 최소/최대 연결요소 검출 방법을 결합하여 자연 이미지로부터 텍스트 영역 검출의 정확도 및 재현률을 향상할 수 있었다.

• 한국해양바이오학회지
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• 제11권1호
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• pp.29-35
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• 2019

In the present study, the alcoholic and enzymatic extracts of Ecklonia maxima were prepared and their antioxidant activities were evaluated via the free radical scavenging activities. The lyophilized seaweed powder was extracted by 70% ethanol and the resultant extract was obtained, named as the alcoholic extract of E. maxima stem (EMSE) obtained. The residue after EMSE preparation was continuously by Celluclast-assistant extraction and then the obtained enzymatic extract was identified as polysaccharides-rich extract of E. maxima stem (EMSP). The results indicated that the extraction yields of EMSE and EMSP were 2.18% and 5.43%, respectively. In addition, the free radical scavenging activities of EMSE and EMSP were determined using an electron spin resonance (ESR) spectrometer. EMSE containing 5.36% of polyphenol and 1.56% of polysaccharide scavenged DPPH and alkyl radicals at the $IC_{50}$ values of 0.48 and 0.63 mg/mL, respectively. EMSP containing 0.33% of polyphenol and 41.58% of polysaccharide showed at the $IC_{50}$ values of 0.33 and 0.55 mg/mL, respectively. These results demonstrate that EMSP containing a high amount of polysaccharides possesses a strong free radical scavenging activity which might be a potential ingredient in pharmacological and cosmeceutical industries.

• 한국임상수의학회지
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• 제23권3호
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• pp.243-250
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• 2006

The chickens fed with the feeds supplemented with green tea(GT) and lactic acid bacteria(LB) were infected orally with 10,000 oocysts per chicken of E. maxima. The groups administered with the feeds supplemented with GT by 0.5% and 2.0% of feed showed the significant levels of decreasing in the number of oocysts shed for 5 days after E. maxima infection. The feeds supplemented with LB by 0.1% and 0.5% of feed were less effective in reducing the number of the fecal oocyst, compared with the groups administered with GT. To evaluate the immunoregulatory effects of the feed additives, the expression patterns of IL-2 and IFN-r in spleen cells were studied by RT-PCR and ELISA. The higher levels of IL-2 transcripts after E. maxima infection were observed in the groups with n, compared with the groups with LB and the mixture of GT and LB. The $IFN-\gamma$ mRNA bands were observed in the all of experimental groups except the uninfected control. The culture supernatants of Con A-stimulated spleen cells($5{\times}10^6cells/ml$) were measured for the concentration of IL-2 and $IFN-\gamma$ by ELISA. The levels of IL-2 and $IFN-\gamma$ on days 3 and 7 after E. maxima infection were significantly augmented in the groups with n. These results indicated that GT-supplemented feeds resulted in higher reduction of oocyst-shedding and more enhanced immune responses in the chicken infected with E. maxima, as compared with LB-supplemented feeds. According to the results, it was implicative that the supplements could be utilized for development of feed additives for anti-coccidiosis.

• 한국수산과학회지
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• 제30권3호
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• pp.377-387
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• 1997

해수로 유출되고 있는 유기인제 살충, 살균제의 형광 분석법에 의한 finger print식 분석 방법을 개발하기 위해, 벼 농사에 가장 많이 사용되어지는 Hinosan과 Kitazin을 포함하는 10가지의 표준 용액을 조제하여, 형광 특성을 측정하였다. $220nm\~520nm$ 구간의 흡수 파장을 이용하여, 여기 (excitation)시켜 얻은 형광스펙트럼들을 이차원 평면 위의 형광세기 표시를 등고선 모양으로 표시하였다. 이 contour들은 각 조사된 유기인제에 대해 특징적인 형태로 관찰되었다. 조사된 10가지의 유기인제 농약의 형광 contour들은, 봉우리가 한 개로 된 DDVP를 포함한 8가지의 유기인제와 두 개의 봉우리로 나타난 Ateric을 포함한 두 가지의 유기인제 농약으로 나뉘어진다. 또한 최대 흡수파장의 영역별로 보면, 단일한 봉우리를 보이는 많은 유기인제는 약 280nm에, Ateric을 포함한 두 종류의 유기인제는 260nm에서, 그리고 장파장에서 최대 형광파장을 보여준 Monopho, Thaconyl의 최대 흡수파장은 비교적 장파장쪽으로 치우친 300nm와 340nm인, 다른 유기인제의 최대 형광파장과 서로 겹치지 않는 영역에서 나타났다. 그러나, 280nm에서 최대 흡수파장을 나타내는 유기인제 중에 Kitazin, Locsion, Meta는 약 2nm이하의 근소한 차이를 두고 최대 형광세기를 308nm주변에서 나타내었다. 이러한 그룹의 분별을 위해, HPLC-형광 자료를 이용하면 용리시간 차이에 의한 분리와 identification이 가능했다. 형광 contour가 매우 유사한 Kitazin, Locsion과 Meta를 비교하면, Kitazin은 $16\~20$분 대에 나타나는 peak들이 매우 미약할 뿐 아니라, 용리시간 9.6분에 나타난 현저한 단일 peak가 다른 형광 특성이 비슷한 유기인제인 Locsion이나 Meta와의 구분이 용이하게 해 준다. 그러나, Locsion과 Meta는 chromatogram의 용리시간이나, 세부적인 작은 peak 특징은 다르나, 그 pattern이 유사하여, 분석상 불확실성이 내재될 가능성이 있다.

• 대한전자공학회논문지SP
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• 제41권6호
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• pp.109-120
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• 2004

본 논문에서는 다층스케일 에지로부터 특이점 검출을 이용한 블록화 현상 제거 방법을 제안하였다. 블록 부호화된 영상에서 블록화 현상 및 에지와 같은 특이점들은 다층스케일 웨이블릿 변환 영역에서 국부 계수 최대치로 검출된다. 제안한 방법에서는 국부 계수 최대치의 Lipschitz 정칙 상수를 이용하여 블록화 현상 및 에지의 특이점들을 구분하고, 웨이블릿 변환 영역에서 블록화 현상에 의한 특이점들을 영역에 따라 스케일별로 제거한다. 실험 결과로부터 제안한 방법이 기존의 방법에 비하여 객관적 화질 및 주관적 화질 측면에서 보다 우수함을 확인하였다.

• Archives of Plastic Surgery
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• 제45권4호
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• pp.384-387
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• 2018

Blepharoplasty is one of the most commonly performed aesthetic procedures. Surgical complications are rare, but can have severe consequences, such as permanent vision loss. In this report, we describe a patient who developed primary angle-closure glaucoma (ACG) with associated vision loss after a oculoplastic procedure using local anesthesia. So far, six similar cases have been described in the literature. It is believed that acute ACG is triggered by the surgical procedure in patients with predisposing risk factors such as a cataract. Surgical triggering factors include the use of buffered lidocaine/xylocaine with adrenaline/epinephrine, stress, and coverage of the eyes postoperatively. Due to postoperative analgesic use, the clinical presentation can be mild and atypical, leading to a significant diagnostic delay. Acute ACG should therefore be excluded in each patient with postoperative complaints by assessing pupillary reactions. If a fixed mid-wide pupil is observed in an ophthalmologic examination, an immediate ophthalmology referral is warranted. Surgeons should be aware of this rare complication in order to offer treatment at an early stage and to minimize the chance of irreversible vision loss.