Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effects of the Feeds Supplemented with Korean Green Tea and Lactic Acid Bacteria on Infection of Eimeria maxima in Chickens

닭에서 녹차 및 유산균 혼합 사료 투여가 Eimeria maxima의 감염에 미치는 효과

  • Jang, Seung-Ik (Daejeon Metropolitan City' Institute of Health & Environment) ;
  • Chung, Nyun-Ki (Daejeon Metropolitan City' Institute of Health & Environment) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • You, Myung-Jo (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Bae-Keun (College of Veterinary Medicine, Chungnam National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University)
  • Published : 2006.09.30
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Abstract

The chickens fed with the feeds supplemented with green tea(GT) and lactic acid bacteria(LB) were infected orally with 10,000 oocysts per chicken of E. maxima. The groups administered with the feeds supplemented with GT by 0.5% and 2.0% of feed showed the significant levels of decreasing in the number of oocysts shed for 5 days after E. maxima infection. The feeds supplemented with LB by 0.1% and 0.5% of feed were less effective in reducing the number of the fecal oocyst, compared with the groups administered with GT. To evaluate the immunoregulatory effects of the feed additives, the expression patterns of IL-2 and IFN-r in spleen cells were studied by RT-PCR and ELISA. The higher levels of IL-2 transcripts after E. maxima infection were observed in the groups with n, compared with the groups with LB and the mixture of GT and LB. The $IFN-\gamma$ mRNA bands were observed in the all of experimental groups except the uninfected control. The culture supernatants of Con A-stimulated spleen cells($5{\times}10^6cells/ml$) were measured for the concentration of IL-2 and $IFN-\gamma$ by ELISA. The levels of IL-2 and $IFN-\gamma$ on days 3 and 7 after E. maxima infection were significantly augmented in the groups with n. These results indicated that GT-supplemented feeds resulted in higher reduction of oocyst-shedding and more enhanced immune responses in the chicken infected with E. maxima, as compared with LB-supplemented feeds. According to the results, it was implicative that the supplements could be utilized for development of feed additives for anti-coccidiosis.

Keywords

References

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