• ALGAE
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• v.18 no.2
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• pp.121-127
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• 2003

The pyrenoid ultrastructure and distribution of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase in the chloroplasts of Chlamydomonas reinhardtii was studied using the immunogold localization technology with electron microscopy. There were several tubular thylakoids invading in the pyrenoid matrix to form several spokewise channels. The connections between pyrenoid matrix and stroma of chloroplast were the partial of channels. The starch sheath surrounding the pyrenoid was separated into several parts by the connections in transection. Some thylakoids were packed together near the connections in one side of the pyrenoid. Those special structures might be used to transport substance between pyrenoid and stroma of chloroplasts. With the antibody raised against the large subunits of Rubsico from C. protothecoides, the result of the gold immunolocalization of Rubisco in Chlamydomonas reinhardtii showed most of the gold particles heavily labeled the pyrenoid matrix, as well as the starch sheath matrix, and very few in the stroma of chloroplasts. The gold particle density was 880.00 $\pm$ 164.32, 190.00 $\pm$ 152.39 and 9.60 $\pm$ 5.37 ${\mu}m^{-2}$ in pyrenoid matrix, starch sheath and stroma region of chloroplast respectively (background: 5.67 $\pm$ 1.53 ${\mu}m^{-2}$). 99.59% of the total Rubiscos was calculated to be concentrated in the pyrenoid matrix and starch sheath by spatial densities. The gold immunolocalization of Rubisco activase also showed that Rubisco activase was mainly concentrated in the periphery of the pyrenoid and the starch sheath (the density was as high as 229.69 $\pm$ 96.96 ${\mu}m^{-2}$). There were very few gold particles located in the stroma of chloroplasts. These results indicated that pyrenoid surface and starch sheath was the site for Rubisco activation and $CO_2$ fixation, which supported the suggestion that pyrenoids perform photosynthesis function.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.7 s.349
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• pp.50-57
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• 2006

Low-density parity-check (LDPC) codes are recently emerged due to its excellent performance. However, the parity check (H) matrices of the previous works are not adequate for hardware implementation of encoders or decoders. This paper proposes a hybrid parity check matrix which is efficient in hardware implementation of both decoders and encoders. The hybrid H-matrices are constructed so that both the semi-random technique and the partly parallel structure can be applied to design encoders and decoders. Using the proposed methods, the implementation of encoders can become practical while keeping the hardware complexity of the partly parallel decoder structures. An encoder and a decoder are designed using Verilog-HDL and compared with the previous results.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.4
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• pp.369-373
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• 2016

Accurate identification of damping matrix in structures is very important for predicting vibration responses and estimating parameters or other characteristics affected by energy dissipation. In this paper, damping matrix identification method that use normal frequency response functions, which were estimated from complex frequency response functions, is proposed. The complex frequency response functions were obtained from the experimental data of the structure. The nonproportional damping matrix was identified through the proposed method. Two numerical examples (lumped-mass model and cantilever beam model) were considered to verify the performance of the proposed method. As a result, the damping matrix of the nonproportional system was accurately identified.

• Structural Engineering and Mechanics
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• v.58 no.1
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• pp.59-91
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• 2016

Laminated composite shells are commonly used in various engineering applications including aerospace and marine structures. In this paper, using semi-analytical finite strip method, the buckling behavior of laminated composite deep as well as thick shells of revolution under follower forces which remain normal to the shell is investigated. The stiffness caused by pressure is calculated for the follower forces subjected to external fibers in thick shells. The shell is divided into several closed strips with alignment of their nodal lines in the circumferential direction. The governing equations are derived based on first-order shear deformation theory which accounts for through thickness-shear flexibility. Displacements and rotations in the middle surface of shell are approximated by combining polynomial functions in the meridional direction as well as truncated Fourier series with an appropriate number of harmonic terms in the circumferential direction. The load stiffness matrix which accounts for variation of loads direction will be derived for each strip of the shell. Assembling of these matrices results in global load stiffness matrix which may be un-symmetric. Upon forming linear elastic stiffness matrix called constitutive stiffness matrix, geometric stiffness matrix and load stiffness matrix, the required elements for the second step analysis which is an eigenvalue problem are provided. In this study, different parameter effects are investigated including shell geometry, material properties, and different boundary conditions. Afterwards, the outcomes are compared with other researches. By considering the results of this article, it can be concluded that the deformation-dependent pressure assumption can entail to decrease the calculated buckling load in shells. This characteristic is studied for different examples.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.15 no.3
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• pp.463-469
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• 2002

The governing equation and force-displacement rotations of a beam-column element on elastic foundation we derived based on variational approach of total potential energy. An exact static and dynamic 4×4 element stiffness matrix of the beam-column element is established via a generalized lineal-eigenvalue problem by introducing 4 displacement parameters and a system of linear algebraic equations with complex matrices. The structure stiffness matrix is established by the conventional direct stiffness method. In addition the F. E. procedure is presented by using Hermitian polynomials as shape function and evaluating the corresponding elastic and geometric stiffness and the mass matrix. In order to verify the efficiency and accuracy of the beam-column element using exact dynamic stiffness matrix, buckling loads and natural frequencies are calculated for the continuous beam structures and the results are compared with F E. solutions.

• Earthquakes and Structures
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• v.22 no.6
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• pp.609-623
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• 2022

To study the empirical seismic fragility of a reinforced concrete girder bridge, based on the theory of numerical analysis and probability modelling, a regression fragility method of a rapid fragility prediction model (Gaussian first-order regression probability model) considering empirical seismic damage is proposed. A total of 1,069 reinforced concrete girder bridges of 22 highways were used to verify the model, and the vulnerability function, plane, surface and curve model of reinforced concrete girder bridges (simple supported girder bridges and continuous girder bridges) considering the number of samples in multiple intensity regions were established. The new empirical seismic damage probability matrix and curve models of observation frequency and damage exceeding probability are developed in multiple intensity regions. A comparative vulnerability analysis between simple supported girder bridges and continuous girder bridges is provided. Depending on the theory of the regional mean seismic damage index matrix model, the empirical seismic damage prediction probability matrix is embedded in the multidimensional mean seismic damage index matrix model, and the regional rapid prediction matrix and curve of reinforced concrete girder bridges, simple supported girder bridges and continuous girder bridges in multiple intensity regions based on mean seismic damage index parameters are developed. The established multidimensional group bridge vulnerability model can be used to quantify and predict the fragility of bridges in multiple intensity regions and the fragility assessment of regional group reinforced concrete girder bridges in the future.

• BMB Reports
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• v.29 no.5
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• pp.405-410
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• 1996

Previous studies have shown that transforming growth factor beta ($TGF-{\beta}$) is a potent regulator of cell growth and differentiation. To study the effects of $TGF-{\beta}$ on cell morphology and cytoskeleton reorganization, we conducted a survey using Mv1Lu mink lung epithelial cells with antibodies to cytoskeletal proteins and an extracellular matrix protein. While the untreated cells showed a cuboidal shape of typical epithelia, the Mv1Lu cells displayed a drastic shape change in the presence of $TGF-{\beta}$. This alteration was most prominent when near-confluent cells were treated with $TGF-{\beta}$. Since the morphology alteration is known to be accompanied by the reorganization of cytoskeletal proteins in other cell types, we investigated the intracellular distribution of the three major cytoskeletal structures: microfilaments, microtubules, and intermediate filaments. In the microfilament system, $TGF-{\beta}$ induced new stress fiber formation, which was caused primarily by the polymerization of cytoplasmic G-actin. However, $TGF-{\beta}$ appeared not to induce any significant changes in microtubular structures and vimentin filaments as determined by indirect fluorescence microscopy. Finally we confirmed the rapid accumulation of fibronectin by immunoblot analysis and chased the protein locations by immunofluorescence microscopy.

• The Journal of Industrial Distribution & Business
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• v.9 no.1
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• pp.89-97
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• 2018

Purpose - The Regional Comprehensive Economic Partnership (RCEP) among 16 countries including South Korea, the largest free trade agreement in the Asia-Pacific region, will be concluded next year. The participating countries decided to pursue a comprehensive and high -quality agreement, while ensuring flexibility considering development level of each country. In this study, trade structures between nations from 2005 through 2016 were examined to see the impact that this agreement will have on Korea and to come up with effective countermeasures. Research design, data, and methodology - The method of analysis includes the analysis of the trade matrix, which is useful for identifying the dependency of the individual countries on the market in the region and the reciprocal dependency of the member countries on the market, and the index of intensity of trade, which is useful for figuring out the share of trade between the parties in total trade. Results - The results showed that first, the international trade coefficients of Vietnam and Philippines are higher than those of China and Japan. Secondly, the international inducement coefficients between China and Japan were high, and that between Indonesia and Burma were low, indicating that Korea's exports did not have much effect on export increase of these countries. Third, as a result of analyzing Korea's trade intensity, it was found that export intensity and import intensity were greater than 1 in Vietnam and Philippines, which shows that there is a high degree of relational bond with these countries. India and Laos countries still have a low level of relational bond, which indicates that there is room for improvement in economic relations when the agreement is concluded. After the signing of the agreement in the future, more diverse industrial structures should be continuously studied. Conclusions - The analysis of trade matrix, trade structure, trade inducement coefficient and trade intensity between Korea and RCEP participating countries shows that the majority of the countries have the high level of economic relationship with Korea. Korea should drive a harder bargain when negotiating the terms of the RCEP, in comparison with the level of the existing FTA agreement excluding Japan.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.2
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• pp.99-106
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• 2019

A wall-frame structure resists horizontal load by the interaction between the flexural mode of the shear wall and the shear mode of the frame, which implies that the frame deflects only by reverse bending of the columns and girders, and that the columns are axially rigid. However, as the height of frame increases the shear mode of frame changes to flexural mode, which is due to the extension and shortening of the columns. An approximate hand method for estimating horizontal deflection and member forces in high-rise shear wall-frame structures subjected to horizontal loading is presented. The method is developed from the continuous medium theory for coupled walls and expressed in non-dimensional structural parameters. It accounts for bending deformations in all individual members as well as axial deformations in the columns. The deformations calculated from the presented approximate method and matrix analysis by computer program are compared. The presented approximate method is more accurate for the taller structures.

• Wind and Structures
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• v.25 no.5
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• pp.493-506
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• 2017

Wind-induced response behavior of long-span roof structures is very complicated, showing significant contributions of multiple vibration modes. The largest load effects in a huge number of members should be considered for the sake of the equivalent static wind loads (ESWLs). Studies on essential matters and necessary conditions of the universal ESWLs are discussed. An efficient method for universal ESWLs on long-span roof structures is proposed. The generalized resuming forces including both the external wind loads and inertial forces are defined. Then, the universal ESWLs are given by a combination of eigenmodes calculated by proper orthogonal decomposition (POD) analysis. Firstly, the least squares method is applied to a matrix of eigenmodes by using the influence function. Then, the universal ESWLs distribution is obtained which reproduces the largest load effects simultaneously. Secondly, by choosing the eigenmodes of generalized resuming forces as the basic loading distribution vectors, this method becomes efficient. Meanwhile, by using the constraint equations, the universal ESWLs becomes reasonable. Finally, reproduced largest load effects by load-response-correlation (LRC) ESWLs and universal ESWLs are compared with the actual largest load effects obtained by the time domain response analysis for a long-span roof structure. The results demonstrate the feasibility and usefulness of the proposed universal ESWLs method.