• Title/Summary/Keyword: Matrices

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Elution Buffers for Human Enteric Viruses in Vegetables with Applications to Norovirus Detection

  • Moon, Aerie;Ahn, Jaehyun;Choi, Weon Sang
    • Journal of Food Hygiene and Safety
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    • v.28 no.4
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    • pp.287-292
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    • 2013
  • The efficient elution of viruses from contaminated food is a critical step for its detection. In this study, conditions optimal for enteric viral recovery from three leafy vegetables (cabbage, lettuce, sesame leaf) and three root vegetables (carrot, onion, mooli) were analyzed to find common buffers (statistically not different) applicable to the leafy and root vegetables. Viral recovery varied depending on the food matrices or elution buffers. Buffer solutions containing 0.25M threonine / 0.3M NaCl (pH 9.5) or 0.25M glycine / 0.14M NaCl (pH 9.5) could efficiently recover poliovirus from five out of six vegetables. The threonine buffer was applied to one leafy vegetable (sesame) and one root vegetable (carrot) for genogroup II norovirus (NoV) detection. The detection sensitivity was significantly higher from the leafy vegetable compared to the root vegetable. The use of these common elution buffers should facilitate the detection of low levels of NoV and other enteric viruses in a wide range of vegetables.

Fine Structural Study of Coelomic Solitary Spermatogenesis in Urechis unicinctus (개불 (Urechis unicinctus) 체강에서의 단위집단 정자형성(Solitary Spermatogenesis)에 관한 미세구조 연구)

  • Shin, Kil-Sang;Kim, Wan-Jong
    • Applied Microscopy
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    • v.32 no.2
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    • pp.107-119
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    • 2002
  • Early spermatocytes of U. unicinctus are found in cluster floating in the coelomic fluid. The spermatocytes in a cluster form a syncytium or cytoplasmic mass, but there are no indications that the cytoplasmic mass is a component of a somatic cell. This work suggested that this type of spermatogenesis can be subordinated to solitary spermatogenesis in the sense excluding structural and functional support of a somatic cell for sperm developments. The solitary spermatogenesis in U. unicinctus is different in appearances and developmental details of sperm organelles and stage distributions from that of localized spermatogenesis. The acrosomal rudiments and centrioles can be observed in the early single cells of spermatogonia and clearly disclosed in the primary spermatocyte. In the stage of secondary spermatocyte, the acrosomal precursor and the centrioles begin to move to each cytoplasmic poles. The polarities of the organelles are attained at stage of spermatids. The spermatocytes and spermatids are arranged circumferentially along the cytoplasmic mass in which some amorphological cytoplasmic components are included. The spermatids reveal to be detached from the cytoplasmic mass into coelomic fluid. It suggests that the spermatogenesis are progressed in support of coelomic fluid, and the fact take into consideration that the spermatogenic cells can be in vitro cultured without somatic cells and with supplements of coelomic fluid.

A Study on Shape Optimization of Distributed Actuators using Time Domain Finite Element Method (시간유한요소법을 이용한 분포형 구동기의 형상최적화에 관한 연구)

  • Suk, Jin-Young;Kim, You-Dan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.33 no.9
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    • pp.56-65
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    • 2005
  • A dynamic analysis method that freezes a time domain by discretization and solves the spatial propagation equation has a unique feature that provides a degree of freedom on spatial domain compared with the space discretization or space-time discretization finite element method. Using this feature, the time finite element analysis can be effectively applied to optimize the spatial characteristics of distributed type actuators. In this research, the time domain finite element method was used to discretize the model. A state variable vector was used in the discretization to include arbitrary initial conditions. A performance index was proposed on spatial domain to consider both potential and vibrational energy, so that the resulting shape of the distributed actuator was optimized for dynamic control of the structure. It is assumed that the structure satisfies the final rest condition using the realizable control scheme although the initial disturbance can affect the system response. Both equations on states and costates were derived based on the selected performance index and structural model. Ricatti matrix differential equations on state and costate variables were derived by the reconfiguration of the sub-matrices and application of time/space boundary conditions, and finally optimal actuator distribution was obtained. Numerical simulation results validated the proposed actuator shape optimization scheme.

Propagation of Structural Waves along Waveguides with Non-Uniformities Using Wavenumber Domain Finite Elements (국부적 불연속을 갖는 도파관을 따라 전파되는 파동에 대한 파수 영역 유한 요소 해석)

  • Ryue, Jungsoo
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.3
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    • pp.191-199
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    • 2014
  • Wave reflection and transmission characteristics in waveguides are an important issue in many engineering applications. A combined spectral element and finite element (SE/FE) method is used to investigate the effects of local non-uniformities but limited at relatively low frequencies because the SE is formulated by using a beam theory. For higher frequency applications, a method named a combined spectral super element and finite element (SSE/FE) method was presented recently, replacing spectral elements with spectral super elements. This SSE/FE approach requires a long computing time due to the coupling of SSE and FE matrices. If a local non-uniformity has a uniform cross-section along its short length, the FE part could be further replaced by SSE, which improves performance of the combined SSE/FE method in terms of the modeling effort and computing time. In this paper SSEs are combined to investigate the characteristics of waves propagating along waveguides possessing geometric non-uniformities. Two models are regarded: a rail with a local defect and a periodically ribbed plate. In the case of the rail example, firstly, the results predicted by a combined SSE/FE method are compared with those from the combined SSEs in order to justify that the combined SSEs work properly. Then the SSEs are applied to a ribbed plate which has periodically repeated non-uniformities along its length. For the ribbed plate, the propagation characteristics are investigated in terms of the propagation constant.

Cognitive Impairment in the Patients with Mildly Active Systemic Lupus Erythematosus (경증 전신성 홍반성 루프스 환자의 인지기능장애)

  • Kim, Jin-Hee;Lee, Chul;Lee, Chang-Uk;Paik, In-Ho
    • Korean Journal of Psychosomatic Medicine
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    • v.5 no.1
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    • pp.89-96
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    • 1997
  • This study was designed to determine whether cognitive impairment was evident in patients with SLE. Also, it aimed to examine the association of cognitive impairment with other clinical variables. The subjects consisted of 20 patients with mildly active SLE and 20 healthy controls. Methods : A total of 20 SLE patients and 20 normal controls completed a computerized neuropsychological test battery using Vienna Test System. These included Cognitrone test, Continuous attention test, Corsi block tapping test, Standard progressive matrices. Also, neuro-behavioral cognitive status examination was done. The symptom severity of depression was measured with Beck Depression Inventory, Hamilton Depression Rating Scale, and current medications were documented. Disease activity was rated using the SLE diasease activity index (SLEDAI). Results : SLE patients had poorer performance than normal controls on the tests of Cognitrone, attention, nonverbal IQ and memory, independent of age, education, disease activity, steroid use and depression status. Conclusion : Cognitive dysfunction was not uncommon in ambulatory SLE patients as measured by standardized neuropsychological tests. It seemed to occur independently of various clinical variables. These findings would suggest that cognitive dysfunction in SLE may be explained by reflecting subclinical central nervous system(CNS) involvement, rather than coexisting psychological distress due to chronic illness or side effect of medication.

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Method Validation for Monitoring of Agricultural Worker Exposure to Insecticide Fenthion (살충제 Fenthion에 대한 농작업자 노출 측정을 위한 분석/시험방법 검증)

  • Kim, Eun-Hye;Lee, Hye-Ri;Choi, Hoon;Moon, Joon-Kwan;Hong, Soon-Sung;Jeong, Mi-Hye;Park, Kyung-Hun;Lee, Hyo-Min;An, Xue Hua;Kim, Jeong-Han
    • The Korean Journal of Pesticide Science
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    • v.15 no.4
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    • pp.357-365
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    • 2011
  • Exposure measurement of agricultural worker to pesticide is one of important part of health risk assessment of pesticide. Therefore exposure matrices, apparatus, instruments and methods must be validated in advance to field experiment. In this study, method validation with an organophosphorus insecticide fenthion was carried out for exposure monitoring of agricultural worker. LOD and LOQ were 0.01 and 0.05 ng, respectively. Calibration curve linearity ($R^2$ > 0.999) and reproducibility (C.V. < 3%) were also excellent. Recovery at LOQ, 10LOQ and 100LOQ levels from gloves, socks, mask, patch, solid sorbent, glass fiber filter was 76~113% (C.V. < 3%). Trapping efficiency was 95~105% while no breakthrough was observed. Method validation for the exposure monitoring was established successfully through several experiments. Such method validation can be usually performed in laboratory and not much different for each pesticide so that, this techniques will be applied widely in research for pesticide exposure monitoring by combination with body surface area and respiration rates.

Characteristics of Piet Oudolf's Garden Design from the Viewpoint of the Contemporary Trends in the Use of Grasses (그라스(Grasses)류의 현대 활용추세 관점에서 본 피에트 우돌프(Piet Oudolf)의 정원 디자인 특징)

  • Park, Eun-Yeong
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.33 no.3
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    • pp.66-71
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    • 2015
  • Given the recent trend of natural planting, the recognized needs for new landscaping plants that have advantages in terms of climate change and maintenance, and expected increases in demands for grasses in Korea, this study is intended to investigate from the design point of view the techniques to use grasses and their significance through garden design by Piet Oudolf who is attracting international interests with the use of perennial plants and grasses and is leading the trends in modern planting design, thereby answering the question: how to best use grasses in landscaping spaces? The characteristics of Oudolf's garden design using grasses are summarized in the following conclusions: First, Oudolf combines perennial plants and grasses to make one-to-one correspondences or express expanded drifts. Here grasses mainly serve as an element to change over to other spaces or as a connecting element between image transitions. Second, the brown color and texture of grasses represent Oudolf's considerations on the temporal continuity of gardens. They express the lyricism and pictorialism of autumn and winter. Third, grasses serve to set layers in wide areas resulting in discordance between viewpoints and circulations. Oudolf repeatedly cross perennial plants and grasses using matrices, islands and distributed layering. Here grasses are used to express abstractive meanings in the settings of scenes.

Assessments of FLECHT SEASET Unblocked Forced Reflood Tests Using RELAP5/MOD3 (RELAP5/MOD3 코드를 이용한 FLECHT SEASET의 강제 재관수 실험에 대한 평가)

  • Baek, Joo-Seok;Lee, Won-Jae;Lee, Sang-Yong;Kuh, Jung-Eui
    • Nuclear Engineering and Technology
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    • v.24 no.3
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    • pp.297-310
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    • 1992
  • FLECHT SEASET unblocked forced reflood tests are assessed using Apollo version of RELAP5/MOD3 5M5. The main purpose of the study is to examine the code predictability under forced reflood conditions having different initial power levels and flooding rates. Among various test matrices, the assessment calculations are performed for the test numbers 31701 31302, 31203, 31805, 34524, 31021, 34006 and 35807 These have been selected because they have similar initial conditions but different initial peak rod powers or flooding rates. In addition, various sensitivity calculations are performed for test number 31203 on the improved models of RELAP5/MOD3. Those are for the effect of Counter Current Flow Limit (CCFL) option at the outlet junction of the test section, for the effect of grid modelling on the interfacial drag calculations as well as on the heat structure calculations, and for the effect of nodalization and the time step size. The results of sensitivity studies show that the improved models of RELAP5/MOD3 enhance the code predictability. The assessment results show that the RELAP5/MOD3 has a tendency to underpredict the turn around temperature and the turn around time. But RELAP5/MOD3 silghtly overpredicts the turn around temperature for high flooding rate. The results also show that the calculated quenching by RELAP5/MOD3 is delayed with the increase of the rod power or the decrease of the flooding rate.

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Subnational Population Projections of Korea Based on Interregional Migration Forecasting: A Multiregional Cohort-Component Method (지역간 인구이동의 예측을 통한 우리나라 시도별 장래 인구 추계: 다지역 코호트-요인법의 적용)

  • Lee, Sang-Il;Cho, Dae-Heon
    • Journal of the Korean Geographical Society
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    • v.47 no.1
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    • pp.98-120
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    • 2012
  • The main objective of this study is to conduct subnational population projections of Korea based on a multiregional cohort-component method. This objective is accomplished by: (i) establishing a viable framework to implement the multiregional cohort-component method with reference to the Markov chain model and Rogers' multiregional population projection model; (ii) applying the established framework to subnational population projections of Korea, 2005~2030. The main results are twofold. First, the proposed method turns out to be highly valid in a methodological sense, which is seen from a high level of coincidence between the estimated and the observed. Second, the projection results turn out to be highly useful in the sense that interregional migration flow matrices are resulted for projection periods. The projected migration flows are expected to provide invaluable information for an understanding of future population change and for a formulation of policy alternatives. This study is strongly inspired by the multiregional perspective emphasizing the evolution of multiple regional populations interconnected by interregional migration flows rather than the overall national change.

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Performance Evaluations for Leaf Classification Using Combined Features of Shape and Texture (형태와 텍스쳐 특징을 조합한 나뭇잎 분류 시스템의 성능 평가)

  • Kim, Seon-Jong;Kim, Dong-Pil
    • Journal of Intelligence and Information Systems
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    • v.18 no.3
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    • pp.1-12
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    • 2012
  • There are many trees in a roadside, parks or facilities for landscape. Although we are easily seeing a tree in around, it would be difficult to classify it and to get some information about it, such as its name, species and surroundings of the tree. To find them, you have to find the illustrated books for plants or search for them on internet. The important components of a tree are leaf, flower, bark, and so on. Generally we can classify the tree by its leaves. A leaf has the inherited features of the shape, vein, and so on. The shape is important role to decide what the tree is. And texture included in vein is also efficient feature to classify them. This paper evaluates the performance of a leaf classification system using both shape and texture features. We use Fourier descriptors for shape features, and both gray-level co-occurrence matrices and wavelets for texture features, and used combinations of such features for evaluation of images from the Flavia dataset. We compared the recognition rates and the precision-recall performances of these features. Various experiments showed that a combination of shape and texture gave better results for performance. The best came from the case of a combination of features of shape and texture with a flipped contour for a Fourier descriptor.