• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.17 no.4
• /
• pp.351-363
• /
• 2004

Equation of motion of non conservative system considering mass matrix, elastic stiffness matrix, load correction stiffness matrix by circulatory force's direction change and Winkler and Pasternak foundation stiffness matrix is derived. Also stability analysis due to the divergence and flutter loads is performed. And the influence of internal and external damping coefficient on flutter load is investigated applying the quadratic eigen problem solution. Additionally the influence of non-conservative force's direction parameter, internal and external damping and Winkler and Pasternak foundation on the critical load of Beck's and Leipholz's and Hauger's columns are investigated.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.14 no.3
• /
• pp.401-411
• /
• 2001

This paper deals with the vibration analysis of the rectangular plates which are subjected to uniform in-plane stresses and supported on In-homogeneous Pasternak foundation. Two parametric foundation which Winkler foundation parameter and shear foundation parameter considered, is called by the Pasternak foundation. The values of Winkler foundation parameter of central and border zone of plate are chosen as k₁and k₂respectively, and the value of shear foundation is chosen as constant about all zone of plate. After composing global flexural stiffeness matrix, geometrical stiffeness matrix, mass matrix, and the stiffeness matrix of the Pasternak foundation, eigenvalue problems which are composed of these matrices are solved. The result shows that the shear foundation parameter must not be ignore when considering the stiffeness of foundation.

• Aerospace Engineering and Technology
• /
• v.10 no.2
• /
• pp.94-100
• /
• 2011

In this paper, an assessment on the preliminary coupled load analysis results for advanced Low Earth Orbit Earth Observation satellite was performed. Spacecraft FE-model was converted into Craig-Bampton model consisting of mass matrix, stiffness matrix, acceleration transformation matrix, displacement transformation matrix, and it was delivered to the launch vehicle developer. Launch vehicle developer performed a coupled load analysis with launch vehicle model and spacecraft Craig-Bampton model, and the coupled load analysis results were provided to us. From the assessment on the analysis results, it was verified that spacecraft is safe under launch environment.

• Nuclear Engineering and Technology
• /
• v.54 no.9
• /
• pp.3459-3469
• /
• 2022

Matrix composites of n-WO₃/n-Bi₂O₃/PVA with different loadings of n-WO₃/n-Bi₂O₃ mixtures (0-15 wt%) and starch (0 and 3 wt%) were fabricated by using melt-mixing method. The X-ray attenuation capability were evaluated based on mass attenuation coefficient (μ/⍴) using a general diagnostic X-ray machine at 40-100 kVp. The effect of starch addition on the dispersion of the fillers in the PVA matrix were observed by using FESEM through morphological analysis. The fabricated samples have shrunken and caused their thickness to be decreased (0.35 mm-0.55 mm) after the drying process even though fixed thickness (2.0 mm) was set initially. The density and HVL values of the samples with 3 wt% starch was seen lower than samples without starch (0 wt%), however the former have provided improvement in filler dispersion and better X-ray attenuation capability compared to the latter. As conclusion, the matrix composite of n-WO₃/n-Bi₂O₃/PVA with 15 wt% of n-Bi₂O₃, 8 wt% of n-WO₃ and 3 wt% starch can be selected as the best promising candidate for X-ray shielding materials.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.11 no.2 s.54
• /
• pp.57-68
• /
• 2007

The mega frame system is becoming popular for the design and construction of skyscrapers because this system exhibits structural efficiency by allowing high rigidity of the structure while minimizing the amount of structural materials to be used. Since the mega frame system is usually adopted for super high-rise buildings, the comfort of occupants may be main concerns in the practical application of this system. For the enhancement of the serviceability of mega frame structures, a semi-active tuned mass damper (STMD) is developed in this study. To this end, a Magnetorheological (MR) damper is employed replacing passive damper as a semi-active damper to improve the control effect of a conventional TMD. Since a conventional finite element model of mega frame structures has significant numbers of DOFs, numerical simulation for investigation of control performances of a STMD is impossible by using the full-order model. Therefore, a reduced-order system using minimal DOFs, which can accurately represent the dynamic behavior of a mega frame structure, is proposed in this study through the matrix condensation technique To improve the efficiency of the matrix condensation technique, multi-level matrix condensation technique is proposed using the structural characteristics of mega frame structures. The efficiency and accuracy of the reduced-order control proposed in this study and the control performance of a STMD were verified using example structures.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.6
• /
• pp.1708-1714
• /
• 2013

Polyhexamethylene guanidine (PHMG) polymers used as an active ingredient in an antibacterial humidifier disinfectant were reported to cause harm to the human health when inhaled, although physical contact with this material was known to present low toxicity to humans. It is therefore necessary to develop an optimal analysis method which enables detection and analysis of PHMG polymers. MALDI-TOF investigations of PHMG are performed with a variety of matrices, and it is found that CHCA and 2,5-DHB are excellent matrices which well reflects the polymer population even at high mass. For the provided PHMG sample, the number-average ($M_n$) and weight-average ($M_w$) molecular masses were determined to be 744.8 and 810.7, respectively, when the CHCA was used as a matrix. The rank of the matrices in terms of averaged molecular weight was CHCA ~2,5-DHB > 5-NSA > DHAP, THAP > ATT > IAA ~ super-DHB ~ HABA. In addition, PSD of the PHMG oligomer ions exhibited a few unique fragmenation characteristics. The formation of a- and c-type fragments was the major fragmentation pathway, and the 25-Da loss peaks generally accompanied a- and c-type fragments.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.9
• /
• pp.1537-1541
• /
• 2015

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

• Journal of Pharmaceutical Investigation
• /
• v.41 no.2
• /
• pp.91-94
• /
• 2011

The purpose of this study was to investigate the effect of peptide charge on the interaction between peptide and poly(D,L-lactide-co-glycolide) (PLGA) for evaluating mechanism of acylated peptide formation in PLGA matrix. As a model peptide, octreotide, a synthetic somatostatin analogue and active ingredient of commercial PLGA product, was used. The disulfide group of octreotide was reduced with dithiothreitol and the sulfhydryl groups were modified with N-${\beta}$-maleimidopropionic acid (BMPA) to neutralize octreotide with positive charge in physiological conditions. The BMPA-conjugated octreotide was identified by measuring the molecular mass with liquid chromatography-mass spectrometry. In the interaction study with PLGA, native octreotide showed initial adsorption to PLGA and substantial production of acylated peptides (56% of overall peptide), whereas BMPA-conjugated octreotide showed minimal adsorption to PLGA and no acylation products for 42 days. Consequently, the neutralization of octreotide completely inhibited the peptide acylation by preventing interaction of peptide with PLGA. In conclusion, this study demonstrates that the initial polymer interaction of peptide is important step for peptide acylation in PLGA matrix and suggests the modulation of peptide charge as strategy for inhibiting the formation of acylated peptide impurities.

• YAKHAK HOEJI
• /
• v.59 no.3
• /
• pp.113-119
• /
• 2015

Protein phosphorylation is one of most important post-translational modifications (PTMs) and plays an important role in regulation of protein function. Here we develop a method for a global identification of phosphopeptides and phosphorylation sites using nano-LC MS/MS. We compared two separation methods, C4 and strong cation ion exchange (SCX). Before phosphopeptides enrichment with $TiO_2$, total proteins from Rat 1 cells have been separated using C4 column or tryptic peptides of proteins from the cells have been separated using SCX column. Finally, we have detected 52 phosphorylation sites on 41 proteins from SCX method and 375 phosphorylation sites on 252 proteins from C4 method, and determined the function and localization of identified phosphoproteins using DAVID software. In particular, we showed new phosphorylation sites from membrane proteins related to various cell signaling mechanisms. This method may contribute to study global signal networks induced by various signals including ligands and drugs.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1995.09a
• /
• pp.57-71
• /
• 1995

A simple mathematical model for gacilitated mass transport with a fixed site carrier membrane was derived by assuming an instantaneous, microscopic concentration (activity) fluctuation, The concentration fluctuation, developed due to reversible chemical reaction between carrier and solute, could acuse the higher chemical potential gradient and the facilitated transport. For mathematical formulation, an analogy was employed between the mass transfer for the facilitated transport with fixed site carrier membrane and the electron transfer in a parallel resistor-capacitor (RC) circuit. For the single RC model, it was assumed that a single capacitor represented the total carrier and a solute could not inter-diffuse between matrix and carrier, allowing only two diffusional pathways, This assumption was relaxed by adopting a serial combination of the parallel RC circuit. Here, a solute diffuses in two elements (matrix or carrier) can exchange its pathway, exhibiting four diffusional pathways. The current models were examined against experimental data and the agreement was exceptional.