• Title/Summary/Keyword: Marine Science Technology

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Screening and Identification of Bacillus sp. TS0611 from Marine Sediments for Protease Production (단백질 분해효소 생산을 위한 해양 유래 Bacillus sp. TS0611의 탐색과 동정)

  • Jang, Young-Boo;Choi, Gyeong-Lim;Hong, Yu-Mi;Choi, Jong-Duck;Choi, Yeung-Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.5
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    • pp.461-467
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    • 2009
  • A bacterial strain was screened and identified from sea sediments in Tongyeong coastal area in order to obtain proteases or peptidase cleaving C-terminal of glutamic acid. Strain TS0611, which showed the highest activity from 5 isolated protease producing strains, was selected and its properties investigated. Strain TS0611 was a gram-positive rod, $1.2\;{\mu}m$ in cell length, catalase positive, motility-positive, melanin-negative and grew at 15~$50^{\circ}C$, and hydrolyzed gelatin and casein. This strain was identified as Bacillus thuringiensis or Bacillus cereus based on results from the API system(API 50 CHB) which examined saccharides properties, fatty acid composition of cell wall, and 16S rRNA gene sequence.

Molecular Cloning and Expression Pattern of Abalone (Haliotis discus hannai) Myostatin cDNA (참전복(Haliotis discus hannai) Myostatin 유전자의 cDNA 동정 및 발현 분석)

  • Lee, Sang-Beum;Kim, Jeong-Hwan;Jo, Mi-Jin;Oh, Mi-Young;Park, Heum-Gi;Jin, Hyung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.2
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    • pp.139-145
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    • 2009
  • We cloned and sequenced the open reading frame (ORF) cDNA encoding myostatin from the muscle of abalone (Haliotis discus hannai). The ORF cDNA of the abalone myostatin is 1134 bp and encoded 377 amino acid residues that were 60-96% homologous with the amino acids of other organism myostatins. In addition, the ORF contained a conserved proteolytic cleavage site (RXRR) and nine conserved cysteine residues in the C-terminus. Semi-quantitative RT-PCR revealed the presence of myostatin mRNA in various tissues. The strongest expression was observed in the mantle of female abalone, and the gills and heart of male abalone.

Comparison of the Food Quality of Freshwater Rainbow Trout Oncorhynchus mykiss Cultured in Different Regions (생산 지역을 달리한 담수산 무지개송어(Oncorhynchus mykiss)의 식품학적 품질 특성 비교)

  • Kang, Sang In;Kim, Ki Hyun;Lee, Jun Kyu;Kim, Yong Jung;Park, Su-Jin;Kim, Min Woo;Choi, Byeong Dae;Kim, Dongsoo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.2
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    • pp.103-113
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    • 2014
  • This study compared the food quality of freshwater rainbow trout Oncorhynchus mykiss cultured (in Pyeongchang, Pyeongtaek, Jecheon and Geochang) with that of imported salmon Oncorhynchus keta. The proximate composition of the four types of rainbow trout was 74.4-75.7% moisture, 18.3-19.4% crude protein, 3.1-4.3% crude lipid, and 1.3-1.7% ash. There were slight differences in the proximate composition of the four types of rainbow trout. No significant (P>0.05) differences were found in the red color and odor and taste intensities of the four types of rainbow trout based on the Hunter a values, volatile basic nitrogen content, odor intensity using an electronic nose and taste intensity using an electronic tongue. The crude protein, total amino acid, and mineral contents of rainbow trout cultured in Pyeongtaek were superior or similar to those of the trout cultured in the other three places. The rainbow trout cultured in Geochang had the highest hardness, at 858.5 g.

Numerical investigation on VIV suppression of marine riser with triangle groove strips attached on its surface

  • Wang, Wei;Song, Baowei;Mao, Zhaoyong;Tian, Wenlong;Zhang, Tingying
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.11 no.2
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    • pp.875-882
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    • 2019
  • The effects of Triangle Groove Strips (TGS) on Vortex-induced Vibration (VIV) suppression of marine riser are numerically investigated using Computational Fluid Dynamics (CFD) method. The range of Reynolds number in simulations is 4.0 × 104 < Re < 1.2 × 105. The two-dimensional unsteady Reynolds-Averaged Navier-Stokes (RANS) equations and Shear Stress Transport (SST) k-ω turbulence model are used to calculate the flow around marine riser. The Newmark-β method is employed for evaluating the structure dynamics of marine riser. The effect of the height ratio (ε) of TGS on VIV suppression is evaluated. The amplitude responses, frequency responses, vortex patterns and the flow around the structures are discussed in detail. With the increase of the height ratio of TGS, the suppression effect of TGS on VIV suppression is improved firstly and then weakened. When ε=0.04, the suppression effect of TGS is the best. Compared with the VIV responses of smooth marine riser, the amplitude ratio is reduced by 38.9%, the peak of the lift coefficient is reduced by 69% and the peak of the drag coefficient is reduced by 40% when Re=6.0 × 104. With the increase of Reynolds number, the suppression effect of TGS on VIV suppression is improved firstly and then weakened. When the Reynolds number is 7.0 × 104, the amplitude ratio can be reduced by 40.1%. As to the large-amplitude vibration cases, the TGS show nice suppression effect on VIV.

Morphological Features of Marine Dinoflagellates from Jangmok Harbour in Jinhae Bay, Korea: A Case of 30 Species in the Orders Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales

  • Shin, Hyeon Ho;Kim, Eun Song;Li, Zhun;Youn, Joo Yeon;Jeon, Seul Gi;Oh, Seok Jin
    • Korean Journal of Environmental Biology
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    • v.34 no.3
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    • pp.141-150
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    • 2016
  • Most previous studies on dinoflagellates in Korean coastal areas were conducted without morphological descriptions and illustrations of the observed dinoflagellates. This indicates that the species and diversity of dinoflagellates may have been respectively misidentified and underestimated in the past, probably due to cell shrinkage, distortion and loss caused by sample fixation. This study provides information on the morphological observations of four dinoflagellate orders (Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales) from Jangmok Harbour in Jinhae Bay, Korea. The unfixed samples were collected weekly from December 2013 to February 2015. A total of 13 genera and 30 species were identified using light and scanning electron microscopy, although some samples were not clarified at the species level. Harmful dinoflagellates, Prorocentrum donghaiense, Tripos furca, Alexandrium affine, A. fundyense, Akashiwo sanguinea and Cochlodinium polykrikoides, were identified based on the morphological observations. The results also reflect the occurrence and identification of dinoflagellates that had not been previously recorded in Jangmok Harbour.

A new cavitation model considering inter-bubble action

  • Shi, Yazhen;Luo, Kai;Chen, Xiaopeng;Li, Daijin;Jia, Laibing
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.13 no.1
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    • pp.566-574
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    • 2021
  • The process of cavitation involves generation, growth, coalescence, and collapse of small bubbles and is tremendously influenced by bubble-bubble interactions. To understand these interactions, a new cavitation model based on the transport equation is proposed herein. The modified Rayleigh-Plesset equation is analyzed to determine the bubble growth rate by assuming equal-sized spherical bubble clouds. The source term in the transport equation is then derived according to the bubble growth rate with the bubble-bubble interaction. The proposed model is validated by various test simulations, including microscopic bubble cloud evolution as well as macroscopical two- and three-dimensional cavitating flows. Compared with previous models, namely the Kunz and Zwart cavitation models, the newly proposed model does not require adjustable parameters and generally results in better predictions both microscopic and macroscopical cases. This model is more physical.

Present Status and Prospects of Marine Chemical Bioindustries (해양화학생물산업의 현황과 전망)

  • Lee, Sun-Bok;Cho, Sun-Ja;Lee, Shin-Youb;Paek, Kwang-Hyun;Kim, Jeong-A;Chang, Jin-Hwa
    • KSBB Journal
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    • v.24 no.6
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    • pp.495-507
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    • 2009
  • As we move into the 21st century, the importance of marine resources is certain to increase due to the accelerated exhaustion of land resources. For the sustainable development of the world, therefore, we need to develop marine chemical bioindustries which enable us to produce industrial chemicals, advanced materials, fuels, and minerals from marine resources such as seaweeds and seawater. In this review, we selected five marine chemical bioindustries which include 1) seaweed polysaccharide industry, 2) marine advanced materials industry, 3) marine biofuel industry, 4) marine sea salt industry, and 5) deep-sea water industry, and discussed the current status and future prospects of each industry sector. It has been assessed that the future of marine chemical bioindustry looks very promising although there are many needs for more intensive research investments on marine bioprocess development through close cooperation between marine biologists and biochemical engineers.

Analysis of Vessel Traffic in Tokyo Bay Observed by New Remote Radar Network System

  • Okano, Tadashi;Ohtsu, Kohei;Hagiwara, Hideki;Shoji, Ruri;Tamaru, Hitoi;Liu, Shun
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2004.08a
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    • pp.208-216
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    • 2004
  • Since 2000, the authors have been developing remote radar network system to observe the vessel traffic in Tokyo Bay. In December 2002, the first operational remote radar station was set at the National Defense Academy in Yokosuka, and vessel traffic observation was started. However, it was impossible to perform accurate observation in the northern part of Tokyo Bay by this Yokosuka radar station only. In September 2003, the second remote radar station and AIS receiving station were installed at Higashi Ogishima in Kawasaki. This second radar enabled us to carry out accurate observation in that area. Both radars can be remotely controlled from the monitoring station in Tokyo University of Marine Science and Technology. On September 30 and October 1,2003, the vessel traffic observation was carried out using both radars. Combining radar images observed by both radars, the ships' tracks were taken and the dangerous ships were extracted by using SJ value and Bumper Model. The time changes of dangerous ship density in some areas in Tokyo Bay and utilization ratio of the traffic routes were also investigated. In addition, analyzing the AIS date received at Kawasaki station, the positions and speed vectors of the ships equipped with AIS were shown.

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