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http://dx.doi.org/10.5657/kfas.2009.42.5.461

Screening and Identification of Bacillus sp. TS0611 from Marine Sediments for Protease Production  

Jang, Young-Boo (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
Choi, Gyeong-Lim (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
Hong, Yu-Mi (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
Choi, Jong-Duck (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
Choi, Yeung-Joon (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.5, 2009 , pp. 461-467 More about this Journal
Abstract
A bacterial strain was screened and identified from sea sediments in Tongyeong coastal area in order to obtain proteases or peptidase cleaving C-terminal of glutamic acid. Strain TS0611, which showed the highest activity from 5 isolated protease producing strains, was selected and its properties investigated. Strain TS0611 was a gram-positive rod, $1.2\;{\mu}m$ in cell length, catalase positive, motility-positive, melanin-negative and grew at 15~$50^{\circ}C$, and hydrolyzed gelatin and casein. This strain was identified as Bacillus thuringiensis or Bacillus cereus based on results from the API system(API 50 CHB) which examined saccharides properties, fatty acid composition of cell wall, and 16S rRNA gene sequence.
Keywords
Screening; Strain identification; Bacillus sp.; Protease; Properties;
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