Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Screening and Identification of Bacillus sp. TS0611 from Marine Sediments for Protease Production

단백질 분해효소 생산을 위한 해양 유래 Bacillus sp. TS0611의 탐색과 동정

  • Jang, Young-Boo (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Gyeong-Lim (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Hong, Yu-Mi (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Jong-Duck (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Yeung-Joon (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 장영부 (경상대학교 해양식품공학과 / 해양산업연구소) ;
  • 최경임 (경상대학교 해양식품공학과 / 해양산업연구소) ;
  • 홍유미 (경상대학교 해양식품공학과 / 해양산업연구소) ;
  • 최종덕 (경상대학교 해양식품공학과 / 해양산업연구소) ;
  • 최영준 (경상대학교 해양식품공학과 / 해양산업연구소)
  • Published : 2009.10.31
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Abstract

A bacterial strain was screened and identified from sea sediments in Tongyeong coastal area in order to obtain proteases or peptidase cleaving C-terminal of glutamic acid. Strain TS0611, which showed the highest activity from 5 isolated protease producing strains, was selected and its properties investigated. Strain TS0611 was a gram-positive rod, $1.2\;{\mu}m$ in cell length, catalase positive, motility-positive, melanin-negative and grew at 15~$50^{\circ}C$, and hydrolyzed gelatin and casein. This strain was identified as Bacillus thuringiensis or Bacillus cereus based on results from the API system(API 50 CHB) which examined saccharides properties, fatty acid composition of cell wall, and 16S rRNA gene sequence.

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References

  1. Adams D, Gurr S and Hogge J. 2005. Cellular fattyacid analysis of Bacillus thuringiensis var. kurstaki commercial preparations. J Agric Food Chem 53, 512-517 https://doi.org/10.1021/jf0485685
  2. Beg QK and Gupta R. 2003. Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus. mojavensis. Enzyme Microbial Technol 32, 294-304 https://doi.org/10.1016/S0141-0229(02)00293-4
  3. Beksan E, Schieberie P, Robert F, Blank I, Fay SB, Schlichtherle-Cemy H and Hofman T. 2003. Synthesis and sensory characterization of novel umami-tasting glutamate glycoconjugates. J Agric Food Chem 51, 5428-5436 https://doi.org/10.1021/jf0344441
  4. Benson HJ. 1990. A Laboratory manual in general microbiology. In: Microbiological Application. Wm C. Brown Publishers, Dubuque, lA, USA, 56-58
  5. Brar SK, Verma M, Surampalli RD, Valero JR and Surampali RY. 2009. Entomotoxicity, protease and chitinase activity of Bacillus thuringiensis fermented wastewater sludge with a high solids content. Bioresource Technol 100, 4317-4325 https://doi.org/10.1016/j.biortech.2007.09.093
  6. Chauhan B and Gupta R. 2004. Application of statistical experimental design for optimization of alkaline protease from Bacillus sp. RGR-14. Process Biochem 39, 2115-2122 https://doi.org/10.1016/j.procbio.2003.11.002
  7. Choi Y, Hur JS, Choi BD, Konno K and Park JW. 2009. Enzymatic hydrolysis of recovered protein from frozen small croaker and functional properties of its hydrokysates. J Food Sci C17- C24
  8. Cole JR, Chai B, Farris RJ, Wang O, Kulam SA, McGarrell DM, Garrity GM and Tiedje JM. 2005. The Ribosomal Database Project(RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33, D294-D296 https://doi.org/10.1093/nar/gki038
  9. Creusot Nand Gruppen H. 2008. Hydrolysis of whey protein isolate with Bacillus licheniformis protease: Aggregating capacities of peptide fractions. J Agric Food Chem 56, 10332-10339 https://doi.org/10.1021/jf801422j
  10. Dunkel A, Koster J and Hofmann T. 2007. Molecular and sensory characterization of $\gamma$-glytamyl peptides as key contributors to the kokumi taste of edible beans (Phaseolus vulgaris L.). J Agric Food Chem 55, 6712-6719 https://doi.org/10.1021/jf071276u
  11. Dunn MJ 2000. Gel electrophoresis of peptides and proteins. In: Peptide and protein drug analysis, R E Reid, ed. Marcel Dekker Inc., New York, 387-451
  12. Fu XT, You SG and Kim SM. 2008. Characterization of a salt-tolerant acid protease produced by Bacillus megaterium KLP-98 and its potential as fermentation starter for the manufacture of fish sauce J Food Biochem 32, 279-298 https://doi.org/10.1111/j.1745-4514.2008.00155.x
  13. Guindon Sand Gascuel O. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likehood. Syst Biol 52, 696-704 https://doi.org/10.1080/10635150390235520
  14. Haddar A, Bougatef A, Agrebi R, Sallami-Kamoun A and Nasri M. 2009. A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21: Purification and characterization. Process Biochem 44, 29-35 https://doi.org/10.1016/j.procbio.2008.09.003
  15. Han X-Q and Damodaran S. 1998. Purification and characterization of protease Q : A detergent and ureastable serine endopeptidase from Bacillus pumilus. J Agric Food Chem 46, 3596-3603 https://doi.org/10.1021/jf980361m
  16. Hua Y, Jiang B, Mine Y and Mu W. 2008. Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. nov. SK006 isolated from an Asian traditional fermented shrimp paste. J Agric Food Chem 56, 1451-1457 https://doi.org/10.1021/jf0713410
  17. Jin SH and Ryu BH. 1995. Isolation and identification of a bacteriolytic enzyme producing bacterial strain from Pusan costal sea. Kor J Appl Microbial Biotechnol 23, 580-587
  18. Kaneda T. 1968. Fatty acids in the genus Bacillus. J Bacteriol 95, 2210 - 2216
  19. Katsuta A, Adachi K, Matsuda S, Shizuri Y and Kasai H. 2005. Ferrimonas marina sp. Int J Syst Evolution Microbiol 55, 1851-1855 https://doi.org/10.1099/ijs.0.63689-0
  20. Kreig NR and Holt JG. 1989. Bergey's Mannual of Systematic Bacteriology. In: Williams & Wilkins, Baltimore, Maryland, 2451-2492
  21. Kumar S, Tamura K and Nei M. 2004. MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment, Brief Bioinform 5, 150-163 https://doi.org/10.1093/bib/5.2.150
  22. Lee HW, Kim BJ, Hwang SH and Kong JY. 1997. Isolation and identification of marine bacterium Bacillus cereus ASK202 and optimal culture conditions for the production of agarase. Kor J Biotechnol Bioeng 12, 228-235
  23. Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Bioi Chem 193, 265-275 https://doi.org/10.1234/12345678
  24. Manni L, Jellouli K, Agrebi R, Bayouch A and Nasri M. 2008. Biochemical and molecular characterization of a novel calcium-dependent metalloprotease from Bacillus cereus SVI Process. Biochem 43, 522-530
  25. Miller L and Berger T. 1985. Bacteria identification by gas chromatography of whole cell fatty acid. In: Hewlett-Packard Application Note 228-248
  26. Niskanen A, Kiutamo T, Raisanen Sand Raevuori M. 1978. Determination of fatty acid compositions of Bacillus cereus and related bacteria Appl Environ Microbiol 35, 453 - 455
  27. Orphan V, House J, Hinrichs CH, McKeegan KU and DeLong EF. 2001. Methane-consuming Archaea revealed by directly coupled isotopic and phylogenetic analysis, Science 5592, 484-486
  28. Park JS, Hwang KS and Chun JS. 2004. Experiments for screen and identification of microorganisms. In: World Science, Seoul, Korea, 127-143
  29. Puri S, Beg QK and Gupta R. 2002. Optimization of alkaline protease production from Bacillus sp. by response surface methodology. Current Microbiol 44, 286-290 https://doi.org/10.1007/s00284-001-0006-8
  30. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F and Higgins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 25, 4876-4882 https://doi.org/10.1093/nar/25.24.4876
  31. Wrolstad RE. 2000. Current Protocols in Food Analytical Chemistry. In: John Wiley & Sons, Inc. New York, U.S.A., Unit C2.2
  32. Yamashita M, Arai S, Kokubo S, Aso K and Fujimaki M. 1975. Synthesis and characterization of a glutamic acid enriched plastein with greater. J Agric Food Chem 23, 27-30 https://doi.org/10.1021/jf60197a027
  33. Seong HK, Lee WJ, Kim YH and Harm KJ. 1992. Studies on bacterial charaxteristics of Bacillus cereus group LS-1 isolated from Suyeong bay. Kor Jour Micobiol 30, 339-346