• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.155-160
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• 2009

Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

• 동의생리병리학회지
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• 제17권5호
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• pp.1264-1269
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• 2003

The inhibitory effects of the Juglans sinensis walnut ethanol extract (JSWE) on carbon tetrachloide (CCl₄)-induced fibrosis, serum transaminases (GOT and GTP), hepatic glutathione (GSH), serum pro-inflammatory cytokines (IL-1β and IL-6) were investigated in rats. JSWE significantly inhibited on CCl₄-induced fibrosis in dose-dependent manner. Moreover, JSWE significantly inhibited on the serum levels of GOT, GTP, IL-1β, and IL-6 in dose-dependent manner in CCl₄-induced fibrosis rat. However, JSWE markedly increased the production of hepatic GSH in a dose-dependent manner. These results show that JSWE may explain some known biological activities of Juglans sinensis walnut including their anti-fibrotic and anti-inflammatory effects, and is of considerable benefit in the treatment for live diseases.

• Toxicological Research
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• 제20권1호
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• pp.21-29
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• 2004

We used cDNA microarray to assess gene expression profiles in hematopoetic cell line, U-937, exposed to low doses of ionizing irradiation. The 1,000 DNA elements on this array were PCR-amplified cDNAs selected from named human cancer related genes. According to the strength of irradiation, the levels of some gene expression were increased or decreased as dose-dependent manner. The gene expressions of Tubulin alpha, protein kinase, interferon-alpha, -beta, -omega receptor and ras homolog gene family H were significantly increased. Especially, Tubulin gene was shown 2.5 fold up-regulated manner under stress of 500 rad irradiation than 200 rad. On the other hand, fibroblast growth factor 12 and four and a half LIM domains, etc. were significantly down-regu-lated. Also, tumor protein 53(TP53) related genes that p53 inducible protein, tumor protein 53-binding protein looks of little significance as radiation sensitive manner. The radio-sensitivity of tubulin gene etc. that we proposed could be useful to rapid and correct survey for the bio-damage by exposure to low dose irradiation.

• 조명전기설비학회논문지
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• 제17권5호
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• pp.94-102
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• 2003

본 연구에서는 능동 데이터베이스를 이용하여 배전선로의 운전을 자동화하는 방안을 제시하였다. 기존의 선로운 전자동화 시스템에서는 수동형 데이터베이스를 사용으로 인위에 의한 실수로 광역정전 또는 과부하 구간의 파급등이 발생될 우려가 있었다. 그러나 제시된 능동 데이터베이스는 선로운전 자동화 시스템 데이터베이스의 상태를 항상 감시하고 스위치의 상태나 계통의 전류 또는 부하량이 변경되었을 경우 그에 상응하는 일련의 작업을 사용자의 개입없이 능동 규칙이용하여 데이터베이스 스스로 수행하도록 함으로써 인위에의한 실수를 최소화 할 수 있다. 제시된 방식을 실 계통인 서울의 K지점의 모의 배전계통에 적용하여 그 유용성을 입증하였다.

• 한국수산과학회지
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• 제49권3호
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• pp.277-284
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• 2016

In this study, we investigated the quality characteristics of madeleine combined with lyophilized red snow crab Chionoecetes japonicus leg-meat powder (CM). The madeleine was prepared by the addition of wheat flour containing 0, 1, 5, 10, 15 and 20% concentrations of CM. Moisture content, pH, and baking loss rate did not differ significantly among groups. Light coloration significantly decreased in a dose-dependent manner, while red and yellow coloration significantly increased. Hardness, chewiness and gumminess significantly increased in a dose-dependent manner, while cohesiveness, springiness and adhesiveness did not differ from CON (madeleine containing 0% CM). In terms of sensory characteristics, appearance and color did not differ compared to CON, while taste, flavor and overall preference increased in a dose-dependent manner, with CM10 having the highest score among the groups. The present study assesses the potential of this improved formula as a convenience food.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.355-361
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• 2011

Effects of aluminum (Al) on plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT), the hepatosomatic index (HSI), and hepatic Al concentration were examined in estradiol-$17{\beta}$ ($E_2$)-administered immature rockfish Sebastes schlegeli. Fish were injected intraperitoneally with $E_2$ (5 mg/kg body weight [BW]) and/or Al (0, 0.1, 1, 5, and 10 mg/kg BW) and plasma and liver samples were extracted 7 days later. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, the relative amount of VTG was determined by integrated optical density. VTG accounted for 23.6% of the total proteins in the control group, but this value decreased with increasing Al administration. Al reduced the concentrations of ALPP and Ca in a concentration-dependent manner and significant reduction occurred at Al concentrations greater than 5 mg/kg. The concentration of GPT increased in a concentration-dependent manner in all Al-administered rockfish. The concentrations of Al in the liver also increased, and HSI was decreased, in a concentration-dependent manner. These results suggest that Al inhibits $E_2$-induced VTG production by being toxic to hepatocytes in marine fish.

• 동의생리병리학회지
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• 제22권1호
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• pp.100-107
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• 2008

The purpose of this research was to investigate the anti-inflammatory effects of Gagamtunong-san(GTNS) which has been medicated the patient such as mastitis. The results were as follows. The cytotoxicity on mouse lung fibroblast Cells(mLFC) was not served at all concentration of GTNS. GTNS in RAW264.7 cell inhibited $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2 and NOS-II mRNA genes expression in a concentration-dependent manner. Specially GTNS inhibited NOS-II production very significantly at 100 ${\mu}g/ml$. GTNS inhibited NO production significantly in a concentration-dependent manner. GTNS inhibited ROS production in a concentration-dependent manner. GTNS inhibited $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production significantly in serum of acute anti-inflammation-induced mice. GTNS increased the survival rate from the 3rd day on LPS-induced lethal endotoxemia. These results suggest that Gagamtunong-san(GTNS) can be useful in treating a lot of women mammary diseases caused by inflammation such as acute and chronic mastitis.

• The Korean Journal of Physiology and Pharmacology
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• 제2권4호
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• pp.419-425
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• 1998

To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ($[Ca^{2+}]_i$) mobilization and cell proliferation were investigated. ATP-induced $[Ca^{2+}]_i$ increased in a dose-dependent manner $(10^{-7}\;M{\sim}10^{-3}\;M)$. ATP-induced $[Ca^{2+}]_i$ increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced $[Ca^{2+}]_i$ increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative $P_{2Y}$ receptor antagonist, dose-dependently weakened ATP-induced $[Ca^{2+}]_i$ mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In conclusion, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2002년도 춘계학술대회 논문집
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• pp.183-188
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• 2002

In locations where the cost or concrete is relatively high, or in situations where the weight or concrete members is to be kept to a minimum, it may be economical to use hollow reinforced concrete vertical members. Hollow reinforced concrete columns with low axial load, moderate longitudinal steel percentage, and a reasonably thick wall were found to perform in a ductile manner at the flexural strength, similar to solid columns. However, hollow reinforced concrete columns with high axial load, high longitudinal steel percentage, and a thin wall were found to behave in a brittle manner at the flexural strength, since the neutral axis is forced to occur away from the inside face of the tube towards the section centroid and, as a result, crushing of concrete occurs near the unconfined inside face of the section. If, however, a steel tube is placed near the inside face of a circular hollow column, the column can be expected not to fail in a brittle manner by disintegration of the concrete in the compression zone. Design recommendation and example by moment-curvature analysis program for curvature ductility are presented. Theoretical moment-curvature analysis for reinforced concrete columns, indicating the available flexural strength and ductility, can be conducted providing the stress-strain relation for the concrete and steel are known. In this paper, a unified stress-stain model for confined concrete by Mander is developed for members with circular sections.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권1호
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• pp.68-82
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• 2011

Wireless mesh networks (WMNs) provide high-speed backbone networks without any wired cable. Many researchers have tried to increase network throughput by using multi-channel and multi-radio interfaces. A multi-radio multi-channel WMN requires channel assignment algorithm to decide the number of channels needed for each link. Since the channel assignment affects routing and interference directly, it is a critical component for enhancing network performance. However, the optimal channel assignment is known as a NP complete problem. For high performance, most of previous works assign channels in a centralized manner but they are limited in being applied for dynamic network environments. In this paper, we propose a simple flow estimation algorithm and a hybrid channel assignment algorithm. Our flow estimation algorithm obtains aggregated flow rate information between routers by packet sampling, thereby achieving high scalability. Our hybrid channel assignment algorithm initially assigns channels in a centralized manner first, and runs in a distributed manner to adjust channel assignment when notable traffic changes are detected. This approach provides high scalability and high performance compared with existing algorithms, and they are confirmed through extensive performance evaluations.