Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Aluminum Inhibits Vitellogenin Production via Toxic Effects on Hepatocytes in the Rockfish Sebastes schlegelii

  • Hwang, Un-Ki (Fisheries Resources and Environment Division, West Sea Fisheries Research Institute (WSFRI)) ;
  • Kang, Han-Seung (Fisheries Resources and Environment Division, West Sea Fisheries Research Institute (WSFRI)) ;
  • Lee, Yoon (Fisheries Resources and Environment Division, West Sea Fisheries Research Institute (WSFRI)) ;
  • Shon, Jae-Kyoung (Fisheries Resources and Environment Division, West Sea Fisheries Research Institute (WSFRI))
  • Received : 2011.08.11
  • Accepted : 2011.11.10
  • Published : 2011.12.31
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Abstract

Effects of aluminum (Al) on plasma vitellogenin (VTG), alkaline-labile phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT), the hepatosomatic index (HSI), and hepatic Al concentration were examined in estradiol-$17{\beta}$ ($E_2$)-administered immature rockfish Sebastes schlegeli. Fish were injected intraperitoneally with $E_2$ (5 mg/kg body weight [BW]) and/or Al (0, 0.1, 1, 5, and 10 mg/kg BW) and plasma and liver samples were extracted 7 days later. After sodium dodecyl sulfate polyacrylamide gel electrophoresis, the relative amount of VTG was determined by integrated optical density. VTG accounted for 23.6% of the total proteins in the control group, but this value decreased with increasing Al administration. Al reduced the concentrations of ALPP and Ca in a concentration-dependent manner and significant reduction occurred at Al concentrations greater than 5 mg/kg. The concentration of GPT increased in a concentration-dependent manner in all Al-administered rockfish. The concentrations of Al in the liver also increased, and HSI was decreased, in a concentration-dependent manner. These results suggest that Al inhibits $E_2$-induced VTG production by being toxic to hepatocytes in marine fish.

Keywords

References

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