• 한국어병학회지
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• 제33권1호
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• pp.35-43
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• 2020

Hypoxia is a serious problem in the marine ecosystem causing a decline in aquatic resources. MicroRNAs (miRNAs) regulate the expression of genes through binding to the corresponding sequences of their target mRNAs. Especially, miRNAs in the cytoplasm can be secreted into body fluids, which called circulating miRNAs, and the availability of circulating miRNAs as biomarkers for hypoxia has been demonstrated in mammals. However, there has been no report on the hypoxia-mediated changes in the circulating miRNAs in fish. miR-210 is known as the representative hypoxia-responsive circulating miRNA in mammals. To know whether fish miR-210 also respond to hypoxia, we analyzed the change of circulating miR-210 quantity in the serum of olive flounder (Paralichthys olivaceus) in response to hypoxia. The expression of hypoxia related genes, hypoxia inducible factor 1α (HIF-1α) and the heat shock protein 90α (HSP90α) was also analyzed. Similar to the reports from mammals, miR-210-5p and miR-210-3p were significantly increased in the serum of olive flounder in response to hypoxia, suggesting that circulating miR-210 levels in the serum can be used as a noninvasive prognostic biomarker for fish suffered hypoxia. The target genes of miR-210 were related to various biological processes, which explains the major regulatory role of miR-210 in response to hypoxia. The expression of HIF-1α and HSP90α in the tissues was also up-regulated by hypoxia. Considering the critical role of HIF-1α in miR-210 expression and HSP90 in miRNAs function, the present up-regulation of HIF-1α and HSP90α might be related to the increase of circulatory miR-210, and the interaction mechanism among HIF-1α, HSP90α, and hypoxia-responsive microRNAs in fish should be further studied.

• Journal of Animal Science and Technology
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• 제60권10호
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• pp.25.1-25.10
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• 2018

The central dogma of gene expression propounds that DNA is transcribed to mRNA and finally gets translated into protein. Only 2-3% of the genomic DNA is transcribed to protein-coding mRNA. Interestingly, only a further minuscule part of genomic DNA encodes for long non-coding RNAs (lncRNAs) which are characteristically more than 200 nucleotides long and can be transcribed from both protein-coding (e.g. H19 and TUG1) as well as non-coding DNA by RNA polymerase II. The lncRNAs do not have open reading frames (with some exceptions), 3`-untranslated regions (3'-UTRs) and necessarily these RNAs lack any translation-termination regions, however, these can be spliced, capped and polyadenylated as mRNA molecules. The flexibility of lncRNAs confers them specific 3D-conformations that eventually enable the lncRNAs to interact with proteins, DNA or other RNA molecules via base pairing or by forming networks. The lncRNAs play a major role in gene regulation, cell differentiation, cancer cell invasion and metastasis and chromatin remodeling. Deregulation of lncRNA is also responsible for numerous diseases in mammals. Various studies have revealed their significance as biomarkers for prognosis and diagnosis of cancer. The aim of this review is to overview the salient features, evolution, biogenesis and biological importance of these molecules in the mammalian system.

• 생태와환경
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• 제49권2호
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• pp.130-141
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• 2016

This study was conducted to elucidate the structure of river ecosystem in the watershed of the Seom River and Hoengseong Reservoir located in Hoengseong of Kangwon Province from February to October 2007. Topics of the survey were mainly rainfall, discharge, water quality in abiotic factors and attached algae, benthic macroinvertebrates, fish, birds and mammals of flora and fauna in a biotic factors, respectively. Specifically, the Seom River could be seen as a typical flow rate of the stream is controlled to the effect of the dam. Basic water qualities were great to seasonal effects, it was relatively clean. Diatom Achnanthes, Cymbella, Gomphonema, and Navicula were distributed predominantly in the periphytic algae. Benthic macroinvertebrates were mostly aquatic insects and freshwater shellfish, the aquatic insects were abundant Ephemeroptera and Trichoptera. Freshwater fish was the dominant Zacco platypus, fish species varied toward the downstream. Birds were mainly observed in four species, and species Egretta garzetta, which was distributed in a wide area of the Seom River. In mammals, Lutra lutra of Mustelidae was identified that the number of inhabit widely. In aspects of the ecological trophic level, the Seom River was maintained at a relatively stable state in the producer and the consumer relationship. The results of this study will expected to be utilized as a useful data for understanding the structure and function of the lotic and lentic ecosystems.

• 한국환경과학회지
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• 제9권3호
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• pp.201-208
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• 2000

PCBs and DDE were determined in the soil samples and the Korean striped field mice, Apodemus agrarius, collected at six localities in Changwon and one locality in Gaduck Island from August 1997 to May 1998. The residual level of PCBs in soils, in which the concentrations of high chlorinated compounds were much higher than those of low chlorinated ones, was highest in the industrial area(IA) in Changwon(60.7 ng/g in average), whereas those of DDE in soils was highest in Gaduck Island(331.9 ng/g in average). However, the levels of each compound detected from soils in the respective remaining places were relatively low. The similar tendency as in the soils was observed in the mice, indicating that the concentration of pollutants in the mice could denote the status of pollution in the terrestrial environment. The composition of PCB congeners in the mice, however, was different from that of soils, but similar to those of other mammals. Moreover, the residual levels of PCBs and DDE in pregnant females from which fetuses were removed were lower than those of males and non-pregnant females. It suggests that these compounds accumulated in pregnant females might have been transferred to the fetuses through placenta as in other mammals. Judging from the above facts, it is suggested that the striped field mouse can be an useful biological index to evaluate the contamination in mammals inhabiting terrestrial environment.

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.327-331
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• 2012

Monthly surveys were conducted to investigate the occurrence of chigger mites and seroprevalence of scrub typhus among small mammals in Jeollanam-do, the southwestern part of Korea, from November 2006 through October 2007. Fifty-eight small mammals, including 57 Apodemus agrarius (98.3%) and 1 Crocidura lasiura (1.7%), were captured, and a total of 4,675 chigger mites representing 4 genera and 8 species were collected from them. The chigger infestation rate among small mammals was 69.0%. The most predominant species in A. agrarius was Leptotrombidium scutellare (54.0%), followed by Leptotrombidium pallidum (39.4%), Leptotrombidium orientale (4.4%), Leptotrombidium palpale (1.1%), Neotrombicula tamiyai (0.6%), Eushoengastia koreaensis (0.3%), Neotrombicula gardellai (0.3%), and Cheladonta ikaoensis (<0.1%). The chigger index of A. agrarius was the highest in October (740.0), followed by November (242.0), September (134.6), March (98.3), February (38.2), January (35.3), December (34.5), April (30.8), and May (1.7). The average antibody positive rate of scrub typhus in wild rodents was 50.0%. The seropositive rates were high in October (100.0%) and November (83.3%), whereas those in other months were relatively low (28.6-57.1%). The chigger index of L. scutellare rapidly increased in September to form an acuminate peak in October, followed by a gradual decline. These results suggest that the outbreak of scrub typhus in the southwestern part of Korean peninsula is mostly due to L. scutellare.

• 한국동물위생학회지
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• 제35권1호
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• pp.33-37
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• 2012

As people's interest about wildlife animal increases, more wildlife animal centers are developing. In South Korea, currently 120 mammal species live, and 22 of them are designated as endangered species. It is important to protect these natural treasures and endangered species, because they are being harmed by human industrial activity. For example, road expansion and poaching have led to the destruction of wildlife animal habitats, which is increasing year by year. Nevertheless, data regarding the systematic treatment of wild animals that are in distress is lacking, and it is a reality that analysis of such data is also lacking. The Kangwon Wildlife Rescue Center investigates these types of distress experienced by mammals. By analyzing the shape of the wild animals killed or injured, the center seeks an efficient and systematic way to manage such distress. The data collected from the Wildlife Animal Center from September 2007 to August 2010 includes 195 different cases, and the animals point of rescue. There are 16 species, including 87 water deer (Hydropotes inermis), 39 raccoons (Nyctereutes procyonoides), 20 roe deer (Capreolus pygargus bedfordi), 10 goral (Nemorhaedus goral), 7 siberian weasels (Mustela sibirica), 6 otters (Lutra lutra) and etc. Within the first year (September 2007 to August 2008), the number of mammals rescued was 46. In the second year (September 2008 to August 2009), 60 animals were rescued. in the third year (September 2009 to August 2010), the Wildlife Animal Center had 89 animals. We can see that these numbers are increasing, which shows that people are interested in the well-being of these animals because they are reporting animal injuries more and more. The mains form of distress caused by humans are from car accidents 67 (34.4%). Traps, and other hunting tools used by poachers are responsible for 25 (13.0%). Also, human industrial activity thus indirectly led to the starvation 25 (13.0%). and the orphaning of young animals 37 (19.0%).

• 한국음향학회지
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• 제40권6호
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• pp.537-545
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• 2021

인간에 의한 연안 지역 개발 및 변형의 가속화로 인해 해양포유류에 대한 피해가 우려되고 있다. 연안 개발이 해양포유류에 어떤 영향을 미칠 수 있는지 이해하려면 이들의 분포를 파악하는 것이 중요하다. 본 연구에서는 서남해 해상풍력 실증단지에서 수동 음향 관측을 통해 상괭이의 출현을 확인하였다. 실험지역에서의 상괭이에 대한 목시관측 결과가 없으므로, 풍력단지에서 탐지된 클릭은 상괭이 발견율이 높은 지역에서 측정한 클릭과 음향특성을 비교하여 검증하였다. 음향 측정 결과에 의하면 상괭이는 매일 출몰하였으며, 평균 클릭수는 40.7회, 상괭이의 출현 비율은 9.7 %, 출현 시간은 18.2 min이며, 대기 시간은 94.9 min으로 나타났다. 이 연구를 통해 서남해 해상풍력 실증단지에서 알려지지 않은 상괭이 출현에 대한 정보를 제공하였으며, 이러한 결과는 풍력단지 운영으로 인한 해양포유류의 영향을 모니터링하는데 도움을 줄 수 있다.

• Molecules and Cells
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• 제47권1호
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• pp.100001.1-100001.8
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• 2024

In eukaryotes, a primary protein quality control (PQC) process involves the destruction of conformationally misfolded proteins through the ubiquitin-proteasome system. Because approximately one-third of eukaryotic proteomes fold and assemble within the endoplasmic reticulum (ER) before being sent to their destinations, the ER plays a crucial role in PQC. The specific functions and biochemical roles of several E3 ubiquitin ligases involved in ER-associated degradation in mammals, on the other hand, are mainly unknown. We identified 2 E3 ligases, ubiquitin protein ligase E3 component N-recognin 1 (UBR1) and ubiquitin protein ligase E3 component N-recognin 2 (UBR2), which are the key N-recognins in the N-degron pathway and participate in the ER stress response in mammalian cells by modulating their stability. Cells lacking UBR1 and UBR2 are hypersensitive to ER stress-induced apoptosis. Under normal circumstances, these proteins are polyubiquitinated through Lys48-specific linkages and are then degraded by the 26S proteasome. In contrast, when cells are subjected to ER stress, UBR1 and UBR2 exhibit greater stability, potentially as a cellular adaptive response to stressful conditions. Although the precise mechanisms underlying these findings require further investigation, our findings show that cytoplasmic UBR1 and UBR2 have anti-ER stress activities and contribute to global PQC in mammals. These data also reveal an additional level of complexity within the mammalian ER-associated degradation system, implicating potential involvement of the N-degron pathway.

• 환경영향평가
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• 제25권6호
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• pp.432-441
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• 2016

본 연구에서는 도심 내 단절되어 있는 서식지의 연결성을 고려하여 생태통로를 구축하고자 하는 선행연구로 서울시의 인왕산과 안산의 생물 종 분포를 예측하였다. 연구대상지역인 인왕산과 안산의 생물 종 분포는 출현자료만으로도 결과를 예측할 수 있는 Maxent(Maximum Entropy Approach) 모형을 이용하여 분석하였다. Maxent 모형을 활용하여 종 분포를 예측하기 위해, 출현자료는 포유류 23개 지점과 박새류(Parus major, P. palustris, P. varius) 3종 15개 지점을 활용하였다. Maxent 모형의 환경변수로는 지형인자 4가지, 식생인자 4가지와 거리인자 2가지를 대상으로 구축하였다. 이 변수들을 활용하여 종 분포를 예측한 결과, 포유류의 경우에는 수치표고자료(DEM)가 34%, 산림지역의 경계로부터 산림내부의 거리가 24.8%, 수종이 10% 순으로 종 분포 모형에 기여도가 높았다. 반면 박새류의 경우에는 수치표고자료가 39.6%, 도로로부터의 거리가 35.4%, 나무의 밀도가 8.2% 순으로 모형에 기여도가 높았다. 따라서 조류 및 포유류는 산림 내부지역을 선호하는 것으로 나타났으며, 이 지역 보전이 필요한 것으로 판단된다.

• 생명과학회지
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• 제28권1호
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• pp.105-109
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• 2018

생물다양성은 생존하는 유기체들의 변이성으로 정의하며 지구상의 생물의 다양성과 생태계를 구성하는 복잡한 상호작용을 일컫는다. 본 연구는 합천군 덕진천에 서식하는 동물의 종조성과 다양성을 공간적 시간적 양상으로 실시하였다. 2016년 시즌에 동정된 포유류, 연골어류, 경골어류, 조류, 양서류, 파충류, 어류 종의 수는 55분류군이었다. 포유류의 Berger-Parker's index (BPI)는 0.233(지점 A)에서 0.333(지점 D)로 나타났다. 포유류와 조류에 대한 Shannon-Weaver index (H')와 두 다른 다양성 척도(N1과 N2)는 상류지역이 하류 지역보다 높았다. 동물 분류군에 대한 ${\beta}$-다양성은 어류 0.229에서 무척추동물 0.339까지 나타났다. 동물종에 대한 풍부도 지수는 지점과 시기에 따라 변화가 있었다. 지점 A는 포유류, 조류, 양서/파충류에서 높은 풍부도를 나타내었다. 비록 다섯 동물 강에 대한 풍부도 지수(R1, R2)가 시즌 별로 다르지만 유의성을 나타내지는 않았다. 균등도 지수(E1-E5) 역시 시즌 별로 다르지만 유의성을 나타내지는 않았다.