• Journal of Ginseng Research
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• v.47 no.2
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• pp.337-346
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• 2023

Background: Ginsenoside Rb2, a major active component of Panax ginseng, has various physiological activities, including anticancer and anti-inflammatory effects. However, the mechanisms underlying the rejuvenation effect of Rb2 in human skin cells have not been elucidated. Methods: We performed a senescence-associated β-galactosidase staining assay to confirm cellular senescence in human dermal fibroblasts (HDFs). The regulatory effects of Rb2 on autophagy were evaluated by analyzing the expression of autophagy marker proteins, such as microtubule-associated protein 1A/1B-light chain (LC) 3 and p62, using immunoblotting. Autophagosome and autolysosome formation was monitored using transmission electron microscopy. Autophagic flux was analyzed using tandem-labeled GFP-RFP-LC3, and lysosomal function was assessed with Lysotracker. We performed RNA sequencing to identify potential target genes related to HDF rejuvenation mediated by Rb2. To verify the functions of the target genes, we silenced them using shRNAs. Results: Rb2 decreased β-galactosidase activity and altered the expression of cell cycle regulatory proteins in senescent HDFs. Rb2 markedly induced the conversion of LC3-I to LC3-II and LC3 puncta. Moreover, Rb2 increased lysosomal function and red puncta in tandem-labeled GFP-RFP-LC3, which indicate that Rb2 promoted autophagic flux. RNA sequencing data showed that the expression of DNA damage-regulated autophagy modulator 2 (DRAM2) was induced by Rb2. In autophagy signaling, Rb2 activated the AMPK-ULK1 pathway and inactivated mTOR. DRAM2 knockdown inhibited autophagy and Rb2-restored cellular senescence. Conclusion: Rb2 reverses cellular senescence by activating autophagy via the AMPK-mTOR pathway and induction of DRAM2, suggesting that Rb2 might have potential value as an antiaging agent.

• Journal of Korean Home Economics Education Association
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• v.34 no.4
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• pp.113-129
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• 2022

The purpose of this study is to propose implications by analyzing the textiles and clothing curriculum of vocational high schools. Literature and data were analyzed, surveys and FGI were conducted, and the following research results were derived. First, many schools chose 'Basic dress design' or 'Basic dress composition' as basic subjects. Through this, it was confirmed that technical training for practice is a priority for vocational schools, and that more discussion is needed regarding the lack of theoretical subjects. Second, similarly to the basic subjects, practical subjects offered are concentrated on 2 to 3 subjects. The teachers asserted that some subjects such as 'Knit apparel production' and the 'Leather and fur design production', which require a lot of equipment and advanced technology, cannot be offered without support from the government. Third, the NCS competency unit of the practical subjects is easy to proceed with the class or is closely concentrated on the type of manpower training presented by each department. Fourth, this study proposes that for technical education to expand students' choice, it is necessary to cultivate teachers competencies linked to the industry. The results of this study are expected to provide basic data on the organization of the next textiles and clothing professional curriculum and help understand vocational education at vocational high schools. In addition, it is believed that this study will contribute to developing and settling various subjects when the high school credit system is implemented by expanding students' choice of major-related subjects.

• Journal of Korea Entertainment Industry Association
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• v.13 no.2
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• pp.93-100
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• 2019

Dynamic Compressors in vocal recordings of modern pop music are essential equipment. Dynamic compressors are applied not only to the mix for listening to music but also to the monitor for the singer to listen to his voice along with the accompaniment while the singer is recording. This study is an experimental study on the effects of a dynamic compressor applied to a monitor environment on the vocal performance of a singer. 10 participating singers participated in the blind test to test how the vocals heard through the monitor would be affected by the 1:1, 2:1 and 4:1 compression ratio. Experimental results show that the higher the compression ratio applied to the monitor, the bigger the song, the brighter the tone, but the pitch becomes finer inaccuracy on the bigger dynamic part of the song. In post-interviews with blinds, it was found that singers generally preferred to hear compressed sound through a compressor on the monitor. Since the music used in the experiment was a ballad with a wide dynamic range, it could not be generalized to all kind of music recordings, but it could provide important implications for the monitoring of recording sites. In addition, We hope that the cognitive science approach to recording technology will be added based on this paper which has been studied through empirical studies on the effect of the monitor environment on the singing voice.

• Environmental and Resource Economics Review
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• v.31 no.4
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• pp.777-799
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• 2022

This study examined the latest EU regulatory strengthening trends for refrigerant gases with very large global warming potential (GWP) and derived implications for carbon neutrality. The European Commission recently unveiled an amendment that significantly strengthens the F-gas Regulation. This study presented the meaning of the main contents related to refrigerants in the amendment by comparing them with the current regulations. The main contents of the amendment include drastically reducing the maximum amount of HFCs that can be placed on the market, strengthening regulations related to HFCs allocation, adding products and equipment that use high GWP refrigerants, adding regulated F-gas and updating the GWP of existing gases, and other stricter regulatory designs. This movement of the EU will affect the policy stance of advanced countries such as the United States and Japan, and Korea's policy will also be further strengthened. Therefore, it will be inevitable for related industries to change to next-generation refrigerant gas. Meanwhile, this study also analyzed the latest policy trends related to per- and polyfluoralkyl substances (PFAS) regulation, which were not noted in previsou studies on refrigerants and F-gas. If PFAS's registration of REACH restricted substances, which are being promoted by five European countries, is made, it will have a very big impact on the industry regarding refrigerant gas. In addition, it will be inevitable to thoroughly review each country's greenhouse gas reduction strategies related to F-gas materials, including refrigerants.

• Korean Chemical Engineering Research
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• v.61 no.1
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• pp.62-67
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• 2023

Due to the prolonged impact of COVID-19, the demand for Information Technology (IT) products is increasing, and their production facilities are expanded. Consequently, the use of harmful and dangerous chemicals are increased, the risk of fire(s) and explosion(s) is also elevated. In order to mitigate these risks, the government sets standards, such as KS C IEC 60079-10-1, and manages explosion-prone hazardous facilities where flammable substances are manufactured, used, and handled. However, using the standards of KS, it is difficult to predict the actual possibility of an explosion in a facility, because ventilation (an important factor) is not considered when setting up a hazardous work environment. In this study, the SEMI S6, Tracer Gas Test was applied to the chemical vapor deposition (CVD) facility, a major part of the display industry, to evaluate ventilation performance and to confirm the possibility of creating a less explosive environment. Based on the results, it was confirmed that the ventilation performance in the assumed scenarios met the standards stipulated in SEMI S6, along with supporting the possibility of creating a less explosive working condition. Therefore, it is recommended to use the prediction tool using engineering techniques, as well as KS standards, in such hazardous environments to prevent accidents and/or reduce economic burden following accidents.

• Nuclear Engineering and Technology
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• v.26 no.1
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• pp.140-148
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• 1994

A qualification test was performed for the iron removal chemical cleaning of the secondary side of nuclear steam generators at the selected temperature, 1$25^{\circ}C$, higher than the standard application temperature, 93$^{\circ}C$. The field cleaning condition for a nuclear unit was tested in a bench scale test loop including a SUS 316 stainless steel autoclave with one gallon capacity as a test vessel. The kinetics of sludge dissolution, corrosion of the secondary side materials and change of solvent chemistry were monitored. Test results indicated that more thorough cleaning was accomplished in less than half of the cleaning time required at 93$^{\circ}C$. And the total corrosions of the secondary side materials were found to be less than the values at 93$^{\circ}C$. While the solvent is recirculated and heated by an external chemical cleaning equipment for the conventional 93$^{\circ}C$ process, the secondary side is heated by the lateral heat of the primary coolant without the recirculation of the cleaning solution, and the solvent is mixed by vigorous boiling induced by periodic ventilation for the high temperature process. The requirement that the reactor coolant pumps should be running during the cleaning operation is the major disadvantage of the high temperature process which also should be considered when chemical cleaning is planned for steam generators under operation.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.10
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• pp.549-564
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• 2016

Due to high cost of domestic production structure, steep growth of China's manufacturing business, and increase in oversea's production, building integrated system to support user-centered product design system based on cognitive and affective information is required to restore development of domestic manufacturing business. This paper put purpose on analysing requirements of end users, especially on information equipment business which works as a major industry in manufacturing businesses, and planning system design direction, prior to constructing user-centered product design support system based on cognitive and affective information. Research was conducted to identify current manufacturing process, application data on manufacturing, availability of cognitive and affective information data and its method of use, and necessity of user-centered product design support system based on cognitive and affective information, by carrying out in-depth interview with 6 related manufacturing companies. Need for user's character information was deducted from the interview, especially cognitive and affective information which is demanding for small to medium manufacturing business to research on its own.

• Informatization Policy
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• v.30 no.4
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• pp.40-61
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• 2023

The adoption of smart factories and smart manufacturing as strategies to enhance competitiveness and stimulate growth in the manufacturing sector is vital for a country's future competitiveness and industrial transformation. The government has consistently pursued smart manufacturing innovation policies starting with the Manufacturing Innovation 3.0 strategy in the Ministry of Industry. This study aims to identify policy areas for smart factories and smart manufacturing based on technical standards. Analyzing policy areas at the current stage where the establishment and support of domestic standards aligning with international technical standards are required is crucial. By prioritizing smart manufacturing process areas within the industry, policymakers can make well-informed decisions to advance smart manufacturing without blindly following international standardization in already well-established areas. To achieve this, the study utilizes a hierarchical analysis method including expert interviews and importance-performance analysis for the five major process areas. The findings underscore the importance of proactive participation in standardization for emerging technologies, such as data and security, instead of solely focusing on areas with extensive international standardization. Additionally, policymakers need to consider carbon emissions, energy costs, and global environmental challenges to address international trends in export and digital trade effectively.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.267-272
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• 2024

Recently, major advanced countries are fostering megacities through policy for reasons such as solving population problems, political and economic issues, and strengthening national competitiveness. The trend of change is accelerating. In Korea, following Seoul and Gyeonggi, mega city policies are being promoted in Busan, Ulsan, Gyeongnam, Daegu and Gyeongbuk, Gwangju and Jeonnam, and Daejeon, Sejong, South Chungcheong and North Chungcheong areas. Due to this urbanization phenomenon, military experts predict that the future battlefield environment will be space or a large city (mega city). From this perspective, Korea will not be able to effectively respond to the threats facing megacities if it does not prepare in advance. Therefore, underground facility operation capabilities optimized for the huge scale of the mega city and the characteristics of the underground operational environment are required. Against this background, the characteristics of the underground operational environment of mega cities and cases of preparation for underground facility operations in advanced military countries such as the United States and Israel were analyzed. Based on this, the capabilities required for underground facility operations suitable for the underground operational environment within Korean megacities are developed from an idea perspective to military organization and combat system, securing special equipment and materials to ensure combatant survival, developing small unit combat techniques, and establishing a training system. It was presented with priority given to.

• Journal of Korean Tunnelling and Underground Space Association
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• v.26 no.3
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• pp.243-254
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• 2024

Currently, due to industrial development in domestic regions, buildings are saturated not only in major city centers but also in surrounding urban areas. Accordingly, people's attention has focused on underground spaces, and tunnels are being widely used, especially in urban development. Research on tunnels and tunnel excavation methods is actively underway. However, there is a lack of research on the wear and tear problems of sludge discharge pipes when using a slurry shield TBM. Therefore, in this paper, the L-shaped bend pipe used in the existing sludge discharge pipe was transformed into a T-shaped bend pipe to move sludge. As a result, it was confirmed that compared to the L-shaped bend pipe, the impact of the T-shaped bend pipe on the bend pipe when discharging sludge was reduced. Based on these results, it is expected that wear of the sludge discharge pipe can be minimized by using a T-shaped bend pipe when using slurry shield TBM equipment. This is expected to ultimately lead to economic benefits, such as reducing costs due to replacement of curved pipes or additional welding during tunnel construction.