• Geomechanics and Engineering
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• v.17 no.2
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• pp.165-173
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• 2019

Plate anchors are generally used for structures like transmission towers, mooring systems etc. where the uplift and lateral forces are expected to be predominant. The capacity of anchor plate can be increased by the use of geosynthetics without altering the size of plates. Numerical simulations have been carried out on three different sizes of square anchor plates. A single layer geosynthetic has been used as reinforcement in the analysis and placed at three different positions from the plate. The effects of various parameters like embedment ratio, position of reinforcement, width of reinforcement, frequency and loading amplitude on the pull out capacity have been presented in this study. The load-displacement behaviour of anchors for various embedment ratios with and without reinforcement has been also observed. The pull out load, corresponding to a displacement equal to each of the considered maximum amplitudes of a given frequency, has been expressed in terms of a dimensionless breakout factor. The pull out load for all anchors has been found to increase by more than 100% with embedment ratio varying from 1 to 6. Finally a semi empirical formulation for breakout factor for square anchors in reinforced soil has also been proposed by carrying out regression analysis on the data obtained from numerical simulations.

• Nuclear Engineering and Technology
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• v.56 no.6
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• pp.2190-2194
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• 2024

The study investigates the dose-dependent direct X-ray sensing characteristics of Brucinium benzilate (BB) and N-acetylglycine (NAG) organic crystals. BB and NAG were prepared as a slurry and deposited as a thick film on a patterned metal electrode. The X-ray induced photocurrent response was examined for various exposure doses using an intraoral pulsed 70 keV X-ray machine connected to a source meter. Subsequently, the morphological properties and thickness of the thick films were analyzed using scanning electron microscopy (SEM). At a photon energy of 70 keV, the attenuation coefficient values for NAG and BB crystals were determined to be approximately 0.181 and 0.178 cm²/g, respectively. The X-ray stopping power of the crystals was measured using a suniray-2 X-ray imaging system. To evaluate the responsiveness of the sensors, the photocurrent sensitivity and noise equivalent dose rate (NED) were calculated for both thick films. The findings demonstrated a noteworthy capability of sensing low doses (mGy), thereby suggesting the potential application of these organic materials in X-ray sensor development.

• Animal Bioscience
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• v.34 no.10
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• Kyungpook Mathematical Journal
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• v.26 no.2
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• pp.163-166
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• 1986

• Kyungpook Mathematical Journal
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• v.32 no.2
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• pp.257-261
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• 1992

• Kyungpook Mathematical Journal
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• v.35 no.1
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• pp.1-15
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• 1995

• Natural Product Sciences
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• v.13 no.3
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• pp.195-198
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• 2007

Hepatoprotective action of alcoholic extract of Bacopa monniera (BME) was evaluated on Dgalactosamine (D-GalN) induced rat liver toxicity. Bacopa monniera extract reduced the elevated serum enzyme activities of ALT, AST, ALP, LDH, ${\gamma}-GT$ and the formation of hepatic malondialdehyde induced by D-GalN. The alcholic extract of Bacopa monniera also significantly restored the decreased levels of glutathione and the decreased activities of glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase and glucose-6-phosphatase. Therefore these results suggest that Bacopa monniera has hepatoprotective effect against D-GalN induced hepatotoxicity.

• Natural Product Sciences
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• v.14 no.2
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• pp.122-126
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• 2008

Chronic oral toxicity studies (90 days) on aqueous and methanol extracts of the whole plant of Peristrophe paniculata (Forssk) Brummitt were carried out in Wistar rats. The dosage was 200 mg/kg/day, p.o. for both the extracts. All external morphological and biochemical changes, in addition to body weight and vital organ weights were recorded. During this investigation, no significant mortality was observed. The results showed that both the extracts were devoid of any toxicity at the dose level studied as compared to the control group.

• Coupled systems mechanics
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• v.1 no.2
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• pp.165-182
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• 2012

Macroscopic hot spots formed due to the large thermal gradients at the surface of the disc brake rotor, make the rotor to fail or wear out early. Thermo-elastic deformation results in contact concentration, leading to the non uniform distribution of temperature making the disc susceptible to hot spot formation. The formation of one hot spot event will predispose the system to future hot spotting at the same location. This leads to the complete thermo-elastic instability in the disc brakes; multitude parameters are responsible for the thermo elastic instability. The predominant factor is the sliding velocity and above a certain sliding velocity the instability of the brake system occurs and hot spots is formed in the surface of the disc brake. Commercial finite element package ABAQUS(R) is used to find the temperature distribution and the result is validated using Rowson's analytical model. A coupled analysis methodology is evolved for the automotive disc brake from the transient thermo-elastic contact analysis. Temperature variation is studied under different sliding speeds within the operation range.

• Journal of the Korean Chemical Society
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• v.58 no.4
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• pp.377-380
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• 2014

A noval derivatives of 3-substituted[1,2,4]triazolo[4,3-b]pyridazine (4a-4o) and 7,8,9,10-tetrahydrobenzo[4,5] thieno[2,3-d][1,2,4]triazolo[4,3-b]pyridazine (7a-7l) is prepared by the reaction of heteroaryl hydrazone from the aldehyde and pyridazinohydrazine derivative, followed by subjecting the intermediate directly to oxidative cyclization employing the mixture of $Me_4NBr$ and Oxone. These derivatives were subjected to preliminary antimicrobial activities against microorganism. All these compounds exhibit good to moderate activity.