• Title/Summary/Keyword: MS-222

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Appropriate in Vitro Culture Conditions of Growing Medium for New Ever-bearing Strawberry 'Goha' (사계성 신품종 딸기 '고하'의 기내배양을 위한 배지의 적정 조건)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Kwon, Young-Seok;Im, Ju-Sung;Yeoung, Young-Rok;Lim, Hak-Tae
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.1051-1056
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    • 2010
  • This study was carried out to determine suitable in vitro culture conditions of new ever-bearing strawberry ($Fragaria$ $ananassa$ Duch.), 'Goha'. Four-week old plantlets which were derived from the meristem culture were used in this study. Three different culture media including MS, Gamborg B5 and White medium were used for the plant culture and the medium concentrations were at the 5 levels of $1/3{\times}$, $1/2{\times}$, $1{\times}$, $2{\times}$ and $3{\times}$. Sucrose content ranged at four levels of 1, 3, 5 and 8% (w/v). Crown diameter on the MS medium was thicker (2.1 mm) than in other media. Fresh weight on the MS medium was 482 mg, which was heavier than 88 mg or 260 mg of Gamborg B5 or White medium, respectively. Particularly, 1/2MS medium was found to have higher growth rate than these in other treatments (fresh weight, dry weight and D/F rate). Shoot length in the treatment of 1% sucrose concentration was 3.6 cm which was the longest. Shoot length was in inverse proportion to the increasing concentration of sucrose. Fresh weight was increased up to 3% sucrose concentration, but decreased above 5% sucrose concentration. From the results, we found that the best condition for in vitro culture of new ever-bearing strawberry 'Goha' was 1/2MS medium supplemented with 1% sucrose concentration.

Analysis of Low Molecular Weight of Seleno compounds in Selenium-Fortified Spirulina (셀레늄 강화 스피룰리나에서의 낮은 분자량 셀레노 화합물 분석)

  • Ji, Young;Lee, Jung Suk;Han, Young-Seok;Pak, Yong N.
    • Journal of the Korean Chemical Society
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    • v.63 no.5
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    • pp.335-341
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    • 2019
  • Spirulina was cultured in Selenium solution and the total concentration was determined with isotope dilution technique. Low-molecular-weight-Selenium species for the water extract of Spirulina were separated and quantified with HPLC ICP/MS. Water extraction was used first and then protein enzyme (protease XIV) was used to digest and extract for the Se species in both water extract and residue. The total Se was $414.9{\pm}4.0{\mu}g\;g^{-1}$ and 77% existed in water extract while 22% remained in residue. Se species in supernatant was mostly inorganic selenate ($222.7{\mu}g\;g^{-1}$). After hydrolysis of protein, SeCys ($15.20{\mu}g\;g^{-1}$) and SeMet ($12.13{\mu}g\;g^{-1}$) were found. In residue, SeCys and SeMet were found with little inorganic Se. After protein hydrolysis of residue, more of Selenoamino acids SeCys ($9.35{\mu}g\;g^{-1}$) and SeMet ($18.23{\mu}g\;g^{-1}$) in addition to MeSeCys ($1.5{\mu}g\;g^{-1}$) were found. It is thought that inorganic selenium is mostly adsorbed on the surface of spirulina and can be easily removed by a simple distilled water extraction while most of organo-seleniums are remained in residue.

Pyrolysis oil refining by Fly-ash absorption (Fly-ash 흡착기법을 이용한 열분해유 정제)

  • Im, EunJung;Kim, SungHyun;Chun, ByungHee;SunWoo, Hwan;Jeong, IckCheol
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.222-222
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    • 2011
  • Plastic product is increasing by the growth of its demand and most of refused plastics are incinerated or reclaimed. However, the refused plastic is not easily decomposed and has the environmental problem with its various toxic gas in case of incineration. Therefore, many countries such as USA, Japan, Germany and other developed industrial countries as well as Korea are interested in studying the recyclable resource of refused plastic. The macromolecular waste pyrolysis has the advantage of collecting of raw materials in high price and can at least get fuel gas or oil with high heat capacity. It also discharges low waste gas and low toxic gas including SOx, NOx and HCl heavy metals. However, pyrolyzed oil includes enough excess unsaturated hydrocarbons to form tar, which can cause the nozzle of engines to plug when pyrolyzed oil is used as fuel. Activated carbon was proven to have prominent adsorption capability among the other adsorbents that were mainly composed of carbon. This study examined the possibility of application in activated charcoal of its solid formation by analysing the feature of pyrolysis which is one of the chemical recycling methods and getting chemical analysis of the product and activated energy. Analyze the element of the oil produced by pyrolysis using GC-MS. The experiment of tar adsorption using fly-ash showed that fly-ash improved the optical intensity of pyrolyzed oil and decreased oxygen compounds in the pyrolyzed oil.

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Comparative Efficacy of 5 Anesthetic Agents in the Glass Catfish Kryptopterus vitreolus (유리메기(Kryptopterus vitreolus)에 대한 최적 마취제 선정을 위한 연구)

  • Lee, Hyo Bin;Jung, Hyo Sun;Ko, Min Gyun;Kim, Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.824-828
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    • 2017
  • We investigated the anesthetic effects of MS-222 (tricaine methanesulfonate), clove oil, 2-phenoxyethanol, $NaHCO_3$, lidocaine-HCl and lidocaine-$HCl/NaHCO_3$ in the glass catfish Kryptopterus vitreolus. Based on the efficacy criteria of complete anesthetic induction from 60 s to 120 s, recovery within 300 s, the lowest effective concentrations at $24^{\circ}C$ were determined to be 60 ppm (induction $82.8{\pm}17.6s$, recovery $80.2{\pm}34.7s$) for MS-222, 40 ppm (induction $70.5{\pm}8.2s$, recovery $83.4{\pm}17.7s$) for clove oil, 250 ppm (induction $64.3{\pm}24.0s$, recovery $62.8{\pm}15.6s$) for 2-phenoxyethanol, 300 ppm (induction $127.3{\pm}13.3s$, recovery $107.5{\pm}4.8s$) for lidocaine-HCl and 200/100 ppm (induction $81.2{\pm}17.2s$, recovery $98.3{\pm}19.7s$) for lidocaine-$HCl/NaHCO_3$. Thus, 200/100 ppm of lidocaine-$HCl/NaHCO_3$ was found to be an effective anesthetic agent.

Measurement and Spatial Analysis of Uranium-238 and Radon-222 of Soil in Seoul

  • Oh, Dal-Young;Shin, Kyu-Jin;Jeon, Jae-Sik
    • Journal of Soil and Groundwater Environment
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    • v.22 no.1
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    • pp.33-40
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    • 2017
  • Identification of radon in soil provides information on the areas at risk for high radon exposure. In this study, we measured uranium-238 and radon-222 concentrations in soil to assess their approximate levels in Seoul. A total of 246 soil samples were taken to analyze uranium with ICP-MS, and 120 measurements of radon in soil were conducted with an in-situ radon detector, Rad7 at a depth of 1-1.5 m. The data were statistically analyzed and mapped, layered with geological classification. The range of uranium in soil was from 0.0 to 8.5 mg/kg with a mean value of 2.2 mg/kg, and the range of radon in soil was from 1,887 to $87,320Bq/m^3$ with a mean value of $18,271Bq/m^3$. The geology had a distinctive relationship to the uranium and radon levels in soil, with the uranium and radon concentrations in soils overlying granite more than double those of soils overlying metamorphic rocks.

Fabrications and Characteristics of Infrared Sensor for Passenger Conditional Detection in Vehicle (차량 내 탑승자 상태 인식용 적외선 센서의 제조 및 특성)

  • Lee, Sung-Hyun;Nam, Tae-Woon
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.22 no.3
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    • pp.222-229
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    • 2009
  • A noble infrared sensor was studied for passenger conditional detection in vehicle, This research relates to uncooled infrared sensors for detecting the presence, type and temperature of occupants in vehicle. It sense that the occupants purpose to control the smart airbag for safety in the case of adult or child and to control the automatic air conditioning for convenience. This paper described the design and the fabrication of microbolometers which were composed of 2 by 8 elements using the surface micromachining technology. The characteristics of the array were investigated in the spectral region of $8{\sim}12{\mu}m$. The fabricated detectors exhibited the thermal mass of $7.05{\times}10^{-9}\;J/K$, the thermal conductance of $1.03{\times}10^{-6}\;W/K$, the thermal time constant of 6.8 ms, the responsivity of $2.96{\times}10^4\;V/W$ and the detectivity of $1.01{\times}10^9\;cmHz^{1/2}/W$, at the chopper frequency of 10 Hz and the bias current of $4.4{\mu}A$. We could successfully detect the human body condition in the divided zone. As a results, we concluded that microbolometer optimized in this research could be useful for the application of passenger conditional detection in vehicle.

Detection of Radiation-Induced Hydrocarbons in Green, Black and Oolong Teas

  • Kausar, Tusneen;Lee, Jung-Eun;Noh, Jung-Eun;Kwon, Young-Ju;Kwon, Joong-Ho
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.222-226
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    • 2004
  • Hydrocarbons induced by gamma-irradiation of green, black, and oolong teas were analyzed to determine whether the hydrocarbons can be used as markers for detecting post-irradiation of these teas. The samples were irradiated at 0, 2.5, 5, 7.5, and 10 kGy. Detection was attempted by extracting fat from the teas, separation of hydrocarbons with florisil column chromatography, and identification of hydrocarbons by gas chromatography-mass spectroscopy (GC-MS). Concentration of hydrocarbons increased with the irradiation dose. The major hydrocarbons in irradiated green, black, and oolong teas were 1-tetradecence (14:1), pentadecane (15:0), 1,7-hexadecadiene (16:2), 1-hexadecene (16:1), 8-heptadecene (17:1), and heptadecane (17:0). Radiation-induced hydrocarbons in teas were 1,7-hexadecadiene and 8-heptadecene. These compounds were not detected in non-irradiated samples, so the hydrocarbons (16:2, 17:1) can be used as markers for detecting post-irradiation of the teas. Furthermore, detection of hydrocarbons after 12 months storage at room temperature remains a suitable method for identifying irradiated teas.

Isolation and Identification of Antimicrobial Compound from Amarantus lividus (참비름 추출물에서 항균성 물질의 분리 및 동정)

  • Oh, Young-Sook;Lee, Shin-Ho
    • Microbiology and Biotechnology Letters
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    • v.33 no.2
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    • pp.123-129
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    • 2005
  • Isolation and identification of pathogens from slaughter and meat processing plant were investigated. Antimicrobial activity of Amaranthus lividus against isolated pathogens such as Aeromonas sobria, Escherichia coli, Escherichia coli O157, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus was investigated. Among the chloroform, ethyl acetate and buthanol fraction of amaranthus lividus showed inhibitory effect against Aeromonas sobria CLFM1 and Escherichia coli CLFM2. Antimicrobial substance in chloroform fraction was isolated by silica gel adsorption column chromatography, sephadex LH-20 column chromatography and silica gel partition column chromatography. The antimicrobial compound of amaranthus lividus was identified as diethyl phtalate by HPLC, GC-MS, H-NMR and C-NMR.

Analysis of COPD Patient's Exhaled Breath Using Sensor Array (센서 어레이를 사용한 COPD 환자의 호기분석)

  • Yu, Joon-Boo;Lee, Shin-Yup;Jeon, Jin-Young;Byun, Hyung-Gi;Lim, Jeong-Ok
    • Journal of Sensor Science and Technology
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    • v.22 no.3
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    • pp.219-222
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    • 2013
  • The exhaled breath contains gases generated from human body. When disease occurs in the body, exhaled breath may include gas components released from disease metabolism. If we can find specific elements through analysis of the exhaled gases, this approach is an effective way to diagnose the disease. The lung function has a close relationship with exhalation. Exhaled gases from COPD (Chronic Obstructive Pulmonary Disease) patients can be analyzed by gas chromatography-mass spectroscopy (GC-MS) and a gas sensor system. The exhaled breath for healthy person and COPD patients had different components. Significantly more benzendicarboxylic acid was detected from COPD patients than in healthy persons. In addition, patients had a variety of decane. Phosphorous compounds with different isomers were detected from patients. The results obtained by gas sensor system were processed by PCA (Principal Component Analysis). The PCA results revealed distinct difference between the patients and healthy people.

A Study of Odorants and Volatiles Released from Pork Belly Meat When Treated by Different Cooking Methods (삼겹살 구이로부터 발생하는 유해물질의 특성 연구)

  • Kim, Bo-Won;Kim, Ki-Hyun;Kim, Yong-Hyun;Ahn, Jeong-Hyeon
    • Journal of Korean Society for Atmospheric Environment
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    • v.30 no.3
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    • pp.211-222
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    • 2014
  • Pork belly meat is one of the most preferred food items for many Korean people. The odorants released from cooking of pork belly meat were measured by three kinds of cooking methods (Charcoal-grill (C), Electric Pan (E), and Gas burner-pan (G)). A total of 16 target compounds including carbonyl compounds, volatile organic compounds, and poly aromatic hydrocarbon were selected and analyzed for comparative purposes. Their emission concentrations were quantified using HPLC-UV, GC-MS, GC-TOF-MS, etc. The gas samples collected by Charcoal-grill cooking showed generally enhanced concentrations of most target compounds among all three kinds of cooking methods. In Charcoal-grill, concentration of benzene, formaldehyde and pyrene went up to 543, 516, and 402 ppb, respectively. It the results are compared in terms of the sum of odor intensity, the highest value (4.25) was also seen from Charcoal-grill. The results of this study confirm that the significantly reduced emission of harmful pollutants can be attained, it pork belly meat is cooked by the Gas or electric pan instead of Charcoal-grill.