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Production of Salidroside from Callus Culture of Rhodiola sachalinensis A. Bor (홍경천(Rhodiola sachalinensis A. Bor)의 캘러스 배양에서 Salidroside생산)

  • Kim, Soo-Jung;Hwang, Baik;Hwang, Sung-Jin;Ahn, Jun-Cheul
    • Journal of Plant Biotechnology
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    • v.31 no.1
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    • pp.89-94
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    • 2004
  • Callus of Rhodiola sachalinensis A. Bor were induced from leaf explant on l/2MS solid supplemented with combination of auxin (2.4-D, NAA: 0.1∼2mg/L) and cytokinin (BA: 0.1∼0.2mg/L). The effects of various medium, culture conditions and phytohormones on the growth of callus were investigated. MS, WPM, B$_{5}$ medium and diluted or concentrated media (1/2X, 2X, 3X) were used to investigate the growth of callus on each media. Among these, the highest growth was observed when cultured in in 2B$_{5}$ medium containing 0.5mg/L NAA and 1mg/L BA, 3% sucrose and 49.6 mM KNO$_3$ as nitrogen source, and 2.16mM NaH$_2$PO$_4$ as phoshate source at $25^{\circ}C$ in the dark. The calli cultured with 5% sucrose produced high salidroside content (0.41% on the basis of dry wt) than normal root (0.17%).

Plant Regeneration by Anther Culture of Tetraploid Populus alba L.X P.glandulosa Uyeki (4배체 현사시나무 (Populus alba L. X P. gludulosa Uyeki)의 약배양에 의한 식물체 재분화)

  • Son, Sung-Ho;Kim, Jung-Hee;Moon, Heung-Kyu;No, Eun-Woon;Lee, Yoon-Hee;Kim, Mi-Hee;Park, Jin-Sun;Lee, Yong-Wook;Yoon, Yang;Lee, Seok-Gu
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.121-126
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    • 1995
  • Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

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Design and Implementation of Real-time Implanted Kernel, RTiK to Support Real-time for a Test Set based on Windows (윈도우 기반의 점검장비에 실시간성을 지원하는 실시간 이식 커널의 설계 및 구현)

  • Lee, Jin-Wook;Cho, Moon-Haeng;Kim, Jong-Jin;Jo, Han-Moo;Park, Young-Soo;Lee, Cheol-Hoon
    • The Journal of the Korea Contents Association
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    • v.10 no.10
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    • pp.36-44
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    • 2010
  • Recently, as new weapons are being developed, test equipments to test their functions inevitably require real-time features. However, since test equipments based on Windows can not support real-time requirements, we have no choice but to use third-party solutions such as RTX or INtime. This leads to increase the development cost of each test equipment. This paper suggests an real-time implemented kernel(RTiK) which operates as a device driver on Windows. RTiK provides another timer using the Local APIC of x86 microprocessors. It supports real-time requirements by periodically executing the required services using Windows-independent timer interrupts to guarantee task deadlines. To reduce the interrupt latency, we used deferred procedure calls provided by Windows. We also used the export driver to implement and modify user-defined functions without accessing the RTiK internals. Using an oscilloscope, we prove that the RTiK kernel proposed in this paper guarantees up to 0.1ms periods.

Mossbauer Studies of the $H_2$ Reduction Effects On Magnetic Properties of Sr-Ba Substituted Hexgonal Ferrite (치환형 Sr-Ba 육방 페라이트들의 자기적 성질에 수소환원이 미치는 효과에 관한 Mossbauer 분광학적 연구)

  • 박재윤;권명회;이재광
    • Journal of the Korean Magnetics Society
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    • v.9 no.1
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    • pp.35-40
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    • 1999
  • Sr substituted materials for some barium in M-type barium ferrite powder and Co-Ti substituted Sr-Ba hexagonal ferrite powder were prepared by citrate sol-gel method and 2 MOE sol-gel method these hexaferrite particles were reduced for 1hr in the hydrogen gas. The reduction temperatures were varied in the range of 250 $^{\circ}C$ to 500 $^{\circ}C$. X-ray diffraction patterns were measured using diffractometer with Cu $K_{\Alhpa}$ radiation. Mossbauer absorption spectra were measured with a constant acceleration spectrometer. We have focused on studying the origin of increasing $M_s$ by M$\"{o}$ssbauer spectroscopy. Ferrite particles which were sintered at 105$0^{\circ}C$ were found to be typical magnetoplumbite structure and single phase. XRD patterns with varying the reduction temperatures in $Sr_{0.5}Ba_{0.5}Fe_{10}O_{19}$ indicates ferrites particles become composite hexaferrites containing $\alpha$-Fe at T_{red}=350 \;$^{\circ}C$$. On the otherhand, it was found that $Co^{2+}$ ions and $Ti^{4+}$ ions in $Sr_{0.7}Ba_{0.3}Fe_{10}CoTiO_{19}$ prevent from changing $Fe^{3+}$ ions to $\alpha$-Fe during the $H_2$ reduction. Comparing Mossbauer results with XRD results, we have determined most of $\alpha$-Fe are reduced from $4f_{vi}$ sites and 12k sites of $Fe^{3+}$ ions. These $\alpha$-Fe phase bring the induced anisotropy and increase saturation magnetization $M_s$.TEX>.

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Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains

  • Zhang, C.X.;Zhao, X.;Han, F.;Yang, M.F.;Chen, H.;Chida, T.;Shen, S.H.
    • Journal of Microbiology and Biotechnology
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    • v.19 no.4
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    • pp.351-357
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    • 2009
  • Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

Effect of O2 Plasma Treatments of Carbon Supports on Pt-Ru Electrocatalysts

  • Park, Soo-Jin;Park, Jeong-Min;Seo, Min-Kang
    • Bulletin of the Korean Chemical Society
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    • v.31 no.2
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    • pp.331-334
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    • 2010
  • In the present study, carbon supports mixed with purified multi-walled carbon nanotubes (MWNTs) and carbon blacks (CBs) were used to improve the cell performance of direct methanol fuel cells (DMFCs). Additionally, the effect of $O_2$ plasma treatment on CBs/MWNTs supports was investigated for different plasma RF powers of 100, 200, and 300 W. The surface and structural properties of the CBs/MWNTs supports were characterized by FT-IR, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and inductive coupled plasma-mass spectrometer (ICP-MS). The electrocatalytic activity of PtRu/CBs/MWNTs catalysts was investigated by cyclic voltammetry measurement. In the experimental results, the oxygen functional groups of the supports were increased with increasing plasma RF power, while the average Pt particle size was decreased owing to the improvement of dispersibility of the catalysts. The electrochemical activity of the catalysts for methanol oxidation was gradually improved by the larger available active surface area, itself due to the introduction of oxygen functional groups. Consequently, it was found that $O_2$ plasma treatments could influence the surface properties of the carbon supports, resulting in enhanced electrocatalytic activity of the catalysts for DMFCs.

Real Time Monitoring of Energy Efficiency Operation Indicator on Merchant Ships

  • Barro, Ronald Dela Cruz;Kim, Jun-Seong;Lee, Don-Chool
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.3
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    • pp.301-308
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    • 2011
  • International Maritime Organization (IMO) proposed the Energy Efficiency Operation Indicator (EEOI) in 2005 and the Energy Efficiency Design Index (EEDI) in 2008 so as to address emission concern and regulation. Likewise, Ship Energy Efficiency Management Plan (SEEMP) and Greenhouse Gas (GHG) monitoring and management are also becoming an issue lately. This paper introduces the energy efficiency design index (operation indicator) monitoring system (EDiMS) software can continuously monitor $CO_2$, $NO_x$, $SO_x$, and PM values emitted from ship. The accurate inventory of ships GHG can be obtained from base of emission result during the engine shop test trial and the actual monitoring of shaft power and ship speed. In addition, the ability to store all exhaust emission and engine operation data can be applied as the useful tool of the inventory work of air pollution and ship energy management plan for the mitigation or reduction of ship emissions.

Automatic Visual Feature Extraction And Measurement of Mushroom (Lentinus Edodes L.)

  • Heon-Hwang;Lee, C.H.;Lee, Y.K.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.1230-1242
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    • 1993
  • In a case of mushroom (Lentinus Edodes L.) , visual features are crucial for grading and the quantitative evaluation of the growth state. The extracted quantitative visual features can be used as a performance index for the drying process control or used for the automatic sorting and grading task. First, primary external features of the front and back sides of mushroom were analyzed. And computer vision based algorithm were developed for the extraction and measurement of those features. An automatic thresholding algorithm , which is the combined type of the window extension and maximum depth finding was developed. Freeman's chain coding was modified by gradually expanding the mask size from 3X3 to 9X9 to preserve the boundary connectivity. According to the side of mushroom determined from the automatic recognition algorithm size thickness, overall shape, and skin texture such as pattern, color (lightness) ,membrane state, and crack were quantified and measured. A portion of t e stalk was also identified and automatically removed , while reconstructing a new boundary using the Overhauser curve formulation . Algorithms applied and developed were coded using MS_C language Ver, 6.0, PC VISION Plus library functions, and VGA graphic function as a menu driven way.

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Theoretical Calculation and Experimental Verification of the Hf/Al Concentration Ratio in Nano-mixed $Hf_xAl_yO_z$ Films Prepared by Atomic Layer Deposition

  • Kil, Deok-Sin;Yeom, Seung-Jin;Hong, Kwon;Roh, Jae-Sung;Sohn, Hyun-Cheol;Kim, Jin-Woong;Park, Sung-Wook
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.5 no.2
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    • pp.120-126
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    • 2005
  • We have proposed a characteristic method to estimate real composition when multi component oxide films are deposited by ALD. Final atomic concentration ratio was theoretically calculated from the film densities and growth rates for $HfO_2$ and $Al_2O_3$ using ALD processed HfxAhOz mms.W e have transformed initial source feeding ratio during deposition to fins] atomic ratio in $Hf_xAl_yO_z$ films through thickness factors ($R_{HFO_2}$ ami $R_{Al_2O_3}$) ami concentration factor(C) defined in our experiments. Initial source feeding ratio could be transformed into the thickness ratio by each thickness factor. Final atomic ratio was calculated from thickness ratio by concentration factor. It has been successfully confirmed that the predicted atomic ratio was in good agreement with the actual measured value by ICP-MS analysis.

Analysis of V2V Broadcast Performance Limit for WAVE Communication Systems Using Two-Ray Path Loss Model

  • Song, Yoo-Seung;Choi, Hyun-Kyun
    • ETRI Journal
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    • v.39 no.2
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    • pp.213-221
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    • 2017
  • The advent of wireless access in vehicular environments (WAVE) technology has improved the intelligence of transportation systems and enabled generic traffic problems to be solved automatically. Based on the IEEE 802.11p standard for vehicle-to-anything (V2X) communications, WAVE provides wireless links with latencies less than 100 ms to vehicles operating at speeds up to 200 km/h. To date, most research has been based on field test results. In contrast, this paper presents a numerical analysis of the V2X broadcast throughput limit using a path loss model. First, the maximum throughput and minimum delay limit were obtained from the MAC frame format of IEEE 802.11p. Second, the packet error probability was derived for additive white Gaussian noise and fading channel conditions. Finally, the maximum throughput limit of the system was derived from the packet error rate using a two-ray path loss model for a typical highway topology. The throughput was analyzed for each data rate, which allowed the performance at the different data rates to be compared. The analysis method can be easily applied to different topologies by substituting an appropriate target path loss model.