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http://dx.doi.org/10.4014/jmb.0805.346

Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains  

Zhang, C.X. (The Institute of Botany, Chinese Academy of Sciences)
Zhao, X. (The Institute of Botany, Chinese Academy of Sciences)
Han, F. (The Institute of Botany, Chinese Academy of Sciences)
Yang, M.F. (The Institute of Botany, Chinese Academy of Sciences)
Chen, H. (The Institute of Botany, Chinese Academy of Sciences)
Chida, T. (Chemicals Agrochemicals Planning and Development Department)
Shen, S.H. (The Institute of Botany, Chinese Academy of Sciences)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.4, 2009 , pp. 351-357 More about this Journal
Abstract
Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.
Keywords
Bacillus subtilis; Magnaporthe grisea P131; in vitro antifungal activity; proteomics; 2DE-PAGE;
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