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Functional characterization of $P_{2X}/P_{2Y}$ receptor in isolated swine renal artery

  • Kim, Joo-heon;Jeon, Je-cheol;Lee, Sang-kil;Lee, Su-jin;Lee, Younggeon;Won, Jinyoung;Kang, Jae seon;Hong, Yonggeun
    • Korean Journal of Veterinary Research
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    • v.47 no.4
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    • pp.371-378
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    • 2007
  • To understand the role of $PM_{2X}/P_{2Y}$ receptor in cortex region of kidney and renal artery, molecular and functional analysis of $PM_{2X}/P_{2Y}$ receptor by pharmacophysiological skill in conventional swine tissues were performed. In functional analysis of $P_{2Y}$ receptor for vascular relaxation, 2-methylthio adenosine triphosphate, a strong agonist of $P_{2Y}$ receptor, induced relaxation of noradrenaline (NA)-precontracted renal artery in a dose-dependent manner. Strikingly, relaxative effect of ATP, 2-msATP, agonists of $P_{2Y}$ receptor, abolished by treatment of reactive blue 2, a putative $P_{2Y}$ receptor antagonist. In contrast, no significant differences of gene encoding $PM_{2X}/P_{2Y}$ and protein expression in immortalized suprachiasmatic nucleus from brain, primary isolated vascular smooth muscle cells from renal artery of pigs and HEK293 from human embryonic kidney under with/without adenosine triphosphate were observed. Taken together, the relationship between molecular and functional characteristic of $PM_{2X}/P_{2Y}$ receptors in conventional pig should be considered that they are another important factor which regulate the kidney function in swine. Based on this study, we propose the purinergic receptor as well as adrenergic and cholinergic receptors is an essential component of the renal homeostasis.

A Study on the Hardware Architecture of Trinomial $GF(2^m)$ Multiplier (Trinomial $GF(2^m)$ 승산기의 하드웨어 구성에 관한 연구)

  • 변기영;윤광섭
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.41 no.5
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    • pp.29-36
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    • 2004
  • This study focuses on the arithmetical methodology and hardware implementation of low-system-complexity multiplier over GF(2$^{m}$ ) using the trinomial of degree a The proposed parallel-in parallel-out operator is composed of MR, PP, and MS modules, each can be established using the regular array structure of AND and XOR gates. The proposed multiplier is composed of $m^2$ 2-input AND gates and $m^2$-1 2-input XOR gates, and the propagation delay is $T_{A}$+(1+[lo $g_2$$^{m}$ ]) $T_{x}$ . Comparison result of the related multipliers of GF(2$^{m}$ ) are shown by table, it reveals that our operator involve more regular and generalized then the others, and therefore well-suited for VLSI implementation. Moreover, our multiplier is more suitable for any other GF(2$^{m}$ ) operational applications.s.

Real-Time Kernel for Linux based on ARM Processor, RTiKA (Real-Time Implant Kernel For ARMLinux) (ARM 프로세서 기반의 리눅스를 위한 실시간 확장 커널 (RTiKA, Real-Time implant Kernel for ARMLinux))

  • Lee, Seung-Yul;Lee, Sang-Gil;Lee, Cheol-Hoon
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.587-597
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    • 2017
  • Recently, the demand for real-time performance in mobile environment is increasing due to the improvement of hardware performance, however a GPOS(General-Purpose Operating System) such as Android and Linux do not provide real-time performance. We developed RTiK(Real-Time implant Kernel) for this problem, but it has the disadvantage of supporting only x86 Architecture. In this paper, we designed and implemented a RTiKA(Real-Time implanted Kernel for ARM) to support real-time in ARM Linux. We used MCT(Multi-Core Timer) timer which replaces Local APIC Timer for real-time support, and we measured the period of generated real-time task for performance verification and evaluation. As the recent the RTiKA can guarantee the operating of several real-time tasks based on the cycle of 1ms.

Identification of Antifungal Activity Subatnces on Seedborn Disease from Garlic and Taxus Extracts (마늘, 주목의 추출물로부터 종자전염성병에 대한 항균활성물질 동정)

  • Chung, III Min;Paik, Su Bong
    • Analytical Science and Technology
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    • v.12 no.1
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    • pp.47-52
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    • 1999
  • Antifungal substances were isolated and identified from garlic and taxus extracts to develop safe and broad fungicide. The inhibitory effect of seedborn disease of sesame, pepper, radish, chinese cabbage by formulation of antifungal substances was investigated. The antifungal substance isolated through column chromatography from garlic and taxus extracts was confirmed by GC-MS as allicin($C_6H_{10}OS_2$) and taxol($C_{47}H_{51}NO_{14}$) and the quantified content from each extracts by HPLC analysis was 0.62%, 0.29%, respectively. The formulation composed of garlic and taxus extracts controlled effectively the seedborn fungi tested in this study at 10X dilution, but at 100X dilution the inhibitory effect decreased. Phytotoxicity of these formulations did not recognized.

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A Study on The Time For Movement of Myosin Heads by the Twitch Stimulation (Twitch Stimulation에 의한 Myosin Heads 움직임의 시간분석 연구)

  • Kim, Duck-Sool;Jung, Jung-Su;Park, Keun-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.21 no.2
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    • pp.182-189
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    • 2004
  • On contraction of the muscles, marked changes in X-ray reflections are observed, suggesting that conformational changes of contractile molecules and the movement of myosin heads during muscle contraction. Time slice requires tension peak after the onset of stimulation and the height of tension peak depends on the number of twitch cycle. The muscles were stimulated by five successive stimuli at an interval of 80 ms started while the tension was still being exerted by the muscles. The intensity of $I_{11}$, $I_{10}$, $143{\AA}$ and $215{\AA}$ reflection measured with 5ms time resolution and is recorded in isometric tension. The peak height of $I_{11}$ and $143{\AA}$ intensity is changed after the onset of a stimulation $I_i$, and the length of twitch is shortened by successive twitches in the case of stimulation $T_i$. On the other hand, the peak height of In and $215{\AA}$ intensity starts to decrease at the 1st twitch and remains constant at low peak height without appreciable recovery during the contraction term. In the case of successive twitch stimulation, the myosin heads of muscle are once moved from their resting position and never returned to their initial position.

Low System Complexity Bit-Parallel Architecture for Computing $AB^2+C$ in a Class of Finite Fields $GF(2^m)$ (시스템 복잡도를 개선한 $GF(2^m)$ 상의 병렬 $AB^2+C$ 연산기 설계)

  • 변기령;김흥수
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.40 no.6
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    • pp.24-30
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    • 2003
  • This study focuses on the arithmetical methodology and hardware implementation of low system-complexity A $B^2$+C operator over GF(2$^{m}$ ) using the irreducible AOP of degree m. The proposed parallel-in parallel-out operator is composed of CS, PP, and MS modules, each can be established using the array structure of AND and XOR gates. The proposed multiplier is composed of (m+1)$^2$ 2-input AND gates and (m+1)(m+2) 2-input XOR gates. And the minimum propagation delay is $T_{A}$ +(1+$\ulcorner$lo $g_2$$^{m}$ $\lrcorner$) $T_{x}$ . Comparison result of the related A $B^2$+C operators of GF(2$^{m}$ ) are shown by table, It reveals that our operator involve more lower circuit complexity and shorter propagation delay then the others. Moreover, the interconnections of the out operators is very simple, regular, and therefore well-suited for VLSI implementation.

Inactivation of various bacteriophages in wastewater by chlorination; Development of more reliable bacteriophage indicator systems for water reuse (하수 처리 과정의 염소 소독에 대한 여러 박테리오파지들의 저항성 평가; 물 재이용 과정의 안전성 관리를 위한 바이러스 지표미생물의 개발)

  • Bae, Kyung-Seon;Shin, Gwy-Am
    • Journal of Korean Society of Water and Wastewater
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    • v.30 no.3
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    • pp.285-291
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    • 2016
  • There has been an accelerating increase in water reuse due to growing world population, rapid urbanization, and increasing scarcity of water resources. However, it is well recognized that water reuse practice is associated with many human health and ecological risks due to numerous chemicals and pathogenic microorganisms. Especially, the potential transmission of infectious disease by hundreds of pathogenic viruses in wastewater is one of the most serious human health risks associated with water reuse. In this study, we determined the response of different bacteriophages representing various bacteriophage groups to chlorination in real wastewater in order to identify a more reliable bacteriophage indicator system for chlorination in wastewater. Different bacteriophages were spiked into secondary effluents from wastewater plants from three different geographic areas, and then subjected to various doses of free chlorine and contact time at $5^{\circ}C$ in a bench-scale batch disinfection system. The inactivation of ${\phi}X174$ was relatively rapid and reached ~4 log10 with a CT value of 5 mg/L*min. On the other hand, the inactivation of bacteriophage PRD1 and MS2 were much slower than the one for ${\phi}X174$ and only ~1 log10 inactivation was achieved by a CT value of 10 mg/L*min. Overall, the results of this study suggest that bacteriophage both MS2 and PRD1 could be a reliable indicator for human pathogenic viruses for chlorination in wastewater treatment processes and water reuse practice.

Development of Biologically Active Compounds from Edible Plant Sources-X. -Isolation of Lipids from the Flower of Campsis grandiflora K. Schum. and their Inhibitory Effect on FPTase- (식용 식물자원으로부터 활성물질의 탐색-X. -능소화(Campsis grandiflora K. Schum.)로부터 지질화합물의 분리 및 FPTase 저해 효과 측정-)

  • Kim, Dong-Hyun;Song, Myoung-Chong;Han, Kyung-Min;Bang, Myun-Ho;Kwon, Byoung-Mog;Kim, Sung-Hoon;Kim, Dae-Keun;Chung, In-Sik;Park, Mi-Hyun;Baek, Nam-In
    • Applied Biological Chemistry
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    • v.47 no.3
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    • pp.357-360
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    • 2004
  • The flower of Campsis granudiflora K. Schum. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as linolenic acid methyl ester, linoleic acid methyl ester, ${\beta}-sitosterol$ and daucosterol. Daucosterol inhibited FPTase activity with $IC_{50}$ values of $14{\pm}0.04\;{\mu}M$.

Influence of Filler and Cure Systems on Whitening of EPDM Composites by Formation of Metal Salt (충전 시스템과 가교 시스템이 금속염 형성에 의한 EPDM 복합체의 백화에 미치는 영향)

  • Chung, Hye-Seung;Choi, Sung-Seen
    • Elastomers and Composites
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    • v.47 no.3
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    • pp.210-215
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    • 2012
  • Whitening phenomena of the EPDM composites with different inorganic filler compositions which were aged at $90^{\circ}C$ for 7 days in air and tap water atmospheres, respectively, were investigated. The aged samples in tap water containing stearic acid exhibited severe whitening phenomena, while all the samples aged in air did not show any whitening. Depending on the filler compositions, there was no big difference in the whitening phenomena. The whitening materials were analyzed using gas chromatography/mass spectrometry (GC/MS), image analysis, energy-dispersive X-ray spectroscopy (EDX), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). The whitening materials were identified to be salts of stearic acid. The salts of stearic acid were formed by reaction of metal cation in tap water and stearic acid in the sample.

The Characteristics of the ${\beta}\;to\;{\alpha}$ Transformation for Zr-Sn and Zr-Nb Alloys (Zn-Sn 및 Zr-Nb 합금의 ${\beta}{\to}{\alpha}$ 상변태 특성)

  • O, Yeong-Min;Jeong, Heung-Sik;Jeong, Yong-Hwan;Kim, Seon-Jin
    • Korean Journal of Materials Research
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    • v.9 no.12
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    • pp.1222-1228
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    • 1999
  • The effects of Sn and Nb content on the characteristics of ${\beta}$ to ${\alpha}$ phase transformation for Zr were studied by optical microscopy and transmission electron microscopy. The basketweave structures consisted of fine ${\alpha}$-laths were shown in all the air-cooled specimens. As the Nb content increased, the ${\beta}{\to}{\alpha}$+${\beta}$ transformation temperature decreased thus allowing the width of ${\alpha}$-lath, however, did not change with Sn content. While water-quenched pure Zr and Zr-xSn alloys were found to be mailny slipped martensite, water-quenched Zr-xNb alloys showed predominantly a twinned martensite. The transition of slipped martensite to twinned martensite was contributed to the decrease of Ms temperature.

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