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Development of Biologically Active Compounds from Edible Plant Sources-X. -Isolation of Lipids from the Flower of Campsis grandiflora K. Schum. and their Inhibitory Effect on FPTase-  

Kim, Dong-Hyun (Graduate School of Biotechnology & Plant Metabolism Research Center)
Song, Myoung-Chong (Graduate School of Biotechnology & Plant Metabolism Research Center)
Han, Kyung-Min (Graduate School of Biotechnology & Plant Metabolism Research Center)
Bang, Myun-Ho (Graduate School of Biotechnology & Plant Metabolism Research Center)
Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology, KIST)
Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Dae-Keun (Department of Pharmacy, Woosuk University)
Chung, In-Sik (Graduate School of Biotechnology & Plant Metabolism Research Center)
Park, Mi-Hyun (Erom Life Co. Ltd.)
Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center)
Publication Information
Applied Biological Chemistry / v.47, no.3, 2004 , pp. 357-360 More about this Journal
Abstract
The flower of Campsis granudiflora K. Schum. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as linolenic acid methyl ester, linoleic acid methyl ester, ${\beta}-sitosterol$ and daucosterol. Daucosterol inhibited FPTase activity with $IC_{50}$ values of $14{\pm}0.04\;{\mu}M$.
Keywords
Campsis grandiflora K. Schum.; linoleic acid; ${\beta}-sitosterol$; daucosterol; FPTase;
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