• Korean Journal of Plant Tissue Culture
• /
• v.26 no.3
• /
• pp.163-169
• /
• 1999

This study was conducted to produce herbicide resistant plants by transferring phosphinothricin acetyltransferase (PAT) gene into Populus alba $\times$ Populus glandulosa No .3 using Agrobacterium tumefaciens MP 90/PAT. Leaf segments from in vitro grown shoots of hybrid poplar No. 3 were soaked in a AB medium containing Agrobacterium tumefaciens MP 90/PAT for 10 min and cocultivated for 2 days on MS medium containing 1.0 mg/L 2,4-D and 0.2mg/L kinetin (CIM). Putative transformed calli could be selected after cocultivation of leaf segments on CIM supplemented with 50mg/L kanamycin and 500mg/L cefotaxime for 3 weeks. The selected calli were cultured on CIM supplemented with 50 mg/L kanamycin and 500 mg/L cefotaxime for 5~8 weeks before transfer to WPM containing 1.0mg/L zeatin, 0.1mg/L BAP, 50 mg/L kanamycin and 500mg/L cefotaxime for shoot regeneration. Shoots were regenerated from the callus after 4 week cultivation, and the regenerants were grown on the same medium for 7~l0 weeks. The plants rooted on 1/2 WPM containing 0.2 mg/L IBA and 50 mg/L kanamycin. To confirm the gene insertion into plants, GUS activity was detected by histochemical assay in the transformed plants. Finally, the presence of both NPT II and PAT genes from the transgenic plants were confirmed by PCR amplification with the gene specific primers and subsequent PCR-Southern blot with DIG-labeled PAT gene probe. After acclimatization in pots for 4 weeks, the plants were sprayed by 3 mL/L of Basta to test resistance to the herbicide. The transgenic plants remained green, whereas all the control plants died after one week.

• Korean Journal of Weed Science
• /
• v.17 no.4
• /
• pp.390-399
• /
• 1997

This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

• Proceedings of the Ginseng society Conference
• /
• 2004.12a
• /
• pp.50-52
• /
• 2004

Introduce of gene connected with disease and transformation system of ginseng, Squalene systhase(PSS) gene cloned from and disease resistant gene were carried out for expression and transformation of plant using Agrobacterium. PSS of 35S-35S-AMV-PSS-Tnos, has been constructed which were mobilized into Agrobacterium tumefaciens strain MP 90 disarmed Ti-plasmid. PSS gene were introduced into the binary vector pRD 400. The transgenic ginseg plants were propagated using repetitive secondary embryogenesis and introduced NPTII and PSS genes of the transgenic ginseng were successfully indentified by the PCR and survival test on the medium.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1994.04a
• /
• pp.13-18
• /
• 1994

99.9% 이상의 고순도 산소를 대량으로 생산하기 위하여는 주로 냉동 증류(cryogenic fractionation)가 사용된다[1]. 그러나 의료 분야를 비롯하여 소량의 산소를 필요로 하는 많은 분야에서 요구되는 산소의 순도는 40-90% 범위에 있다[2,3]. 이 정도 등급의 산소의 공급은 PSA(Pressure Swing Adsorption)과 MP(Membrane Permeation)의 공정에 의존하게 될 가능성이 크다[4,6]. 이는 이 공정들이 작은 규모의 생산에 적합할 뿐만 아니라 조업이 용이하고, 유지비가 적게 들며 장치의 재배치가 용이하기 때문이다.

• Digital Contents
• /
• no.5 s.156
• /
• pp.86-90
• /
• 2006

봄이 무르익었다. 봄은 겨우내움츠렸던 몸을 집안 밖으로 이끌어낸다. 두툼한 겨울옷을 벗어던지고 화사하고 가벼워진 봄옷 차림으로 계절을 즐기게 한다. 지난달 중순께에는 난데없는 꽃샘추위와 황사로 고생하기도 했지만, 넉넉하고 포근한 봄 햇살은 아직 우리의 외풀을 기다리고 있다. 본격적인 야외 활동 시즌이다. 밖으로 향하면서도 못내 무언가 허전한 느낌을 지울 수 없다. IT세상에 살고 있다보니 야외에 나가면서도 IT의 끈을 놓지 못하고 정보의 도구를 찾고있는 자신의 모습을 발견할 떄도 있다. 휴대전화는 기본이고, MP3플레이어나 PMP등의 멀티미디어 재생도구, 디지털카메라와 같은 기기들을 휴대하고 다니는 것은 이제 일반화되었다.최근에는 PC마저도 사용자의 외출에 동참하고 있다. 이번 호에서는 봄 나들이를 떠나는 PC의 화려한 외출에 대해서 이야기하고자 한다.

• The korean journal of orthodontics
• /
• v.48 no.6
• /
• pp.349-356
• /
• 2018

Objective: This study was performed to investigate the alveolar bone of lower incisors in skeletal Class III adults of different vertical facial patterns and to compare it with that of Class I adults using cone-beam computed tomography (CBCT) images. Methods: CBCT images of 90 skeletal Class III and 29 Class I patients were evaluated. Class III subjects were divided by mandibular plane angle: high (SN-MP > $38.0^{\circ}$), normal ($30.0^{\circ}$ < SN-MP < $37.0^{\circ}$), and low (SN-MP < $28.0^{\circ}$) groups. Buccolingual alveolar bone thickness was measured using CBCT images of mandibular incisors at alveolar crest and 3, 6, and 9 mm apical levels. Linear mixed model, Bonferroni post-hoc test, and Pearson correlation analysis were used for statistical significance. Results: Buccolingual alveolar bone in Class III high, normal and low angle subjects was not significantly different at alveolar crest and 3 mm apical level while lingual bone was thicker at 6 and 9 mm apical levels than on buccal side. Class III high angle group had thinner alveolar bone at all levels except at buccal alveolar crest and 9 mm apical level on lingual side compared to the Class I group. Class III high angle group showed thinner alveolar bone than the Class III normal or low angle groups in most regions. Mandibular plane angle showed negative correlations with mandibular anterior alveolar bone thickness. Conclusions: Skeletal Class III subjects with high mandibular plane angles showed thinner mandibular alveolar bone in most areas compared to normal or low angle subjects. Mandibular plane angle was negatively correlated with buccolingual alveolar bone thickness.

• Applied Chemistry for Engineering
• /
• v.8 no.5
• /
• pp.749-755
• /
• 1997

Molybdenum phosphate(P/Mo = 0.6) has been synthesized with ammonium molybdate and phosphoric acid under aqueous solution. The kinetics of the ammoxidation of methylpyrazine over molybdenum phosphate catalyst was investigated with the variation of reaction temperature and partial pressure of methylpyrazine, oxygen and ammonia, respectively at atmospheric pressure. The catalytic activity was constant for 300hrs operation under our experimental conditions. Under the steady-state condition, the rate equation of methylpyrazine was shown as $-r=kP_{MP}P_{NH3}{^0}P_{O2}{^{\gamma}}({\gamma}=2.2;1.3{\leq}P_{O2}(kPa){\leq}4)$. The apparent activation energy was 29.6kcal/mol below 623K. The main product obtained in the ammoxidation of methylpyrazine is cyanopyrazine whose selectively was kept always over 90% regardless of conversion.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.33 no.11A
• /
• pp.1117-1123
• /
• 2008

Applications for mobile multimedia are testing the performance limits of present day CPUs with variety. However, hardwired solutions are inflexible and expensive to develop. CPUs with flexibility have limitation of performance. So, the requirement for both ASIC-like performance and CPU-like flexibility has led to reconfigurable processor. Mobile systems require low power and high performance concurrently. In this paper, we propose reconfigurable processor for mobile multimedia with high energy efficiency. Reconfigurable processor with 121MOPS/mW is developed by 130nm CMOS technology. And the processor was simulated for energy efficiency with 539MOPS/mW by 90nm CMOS technology and effective use of instructions. And we tested its applications for multimedia field. We tested the case of inverse MDCT for MP3 and DF for MPEG4 and ME for H.264.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.4
• /
• pp.197-200
• /
• 2001

Agrobacterium tumefaciens MP90 harboring PAT (phosphinothricin acetyltransferase) and NPTII-GUS gene were used for the genetic transformation of lettuce (Lactuca Sativa L.). Shoot regeneration from cotyledon explants were obtained from the MS medium supplemented with 0.1 mg.L$^{-1}$ NAA, 1.0 mg.L$^{-1}$ 2ip, 50 mg.L$^{-1}$ kanamycin and 500 mg.L$^{-1}$ carbenicillin after cocultivation with A. tumefaciens for 2 days. Kanamycin resistance test of transgenic plants indicated that the NPTII gene was integrated into the lettuce genome and was stably expressed. PCR and northern blot analysis indicated that bialaphos resistance gene (PAT) was stably integrated into the lettuce genome. The transgenic plant sprayed with Basta (1500x) remained healthy with continuous growth, while the control group exhibited fatality.

• Journal of the Korean Society for Precision Engineering
• /
• v.18 no.5
• /
• pp.98-104
• /
• 2001

A dynamic fracture toughness test method with a chevron notched ceramic specimens is proposed. The notch angles of the chevron specimens were 90, 100$^{\circ}$and 110$^{\circ}$. Finite element analysis(FEA) were done to determine the geometrical properties of chevron-notch specimens according to notch angles. The static fracture toughness of the chevron notched alumina specimen was 3.8MP$\alpha$√m similar to that of the general fracture specimen with a precrack. Dynamic fracture toughness was 4.5 MP$\alpha$√m slightly higher than the static one. These research showed the possibility of the split Hopkinson pressure bar test method using the newly proposed chevron notched specimens to get the dynamic fracture toughness of extremely brittle materials such as ceramics.