• Korean Journal of Metals and Materials
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• v.49 no.8
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• pp.628-634
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• 2011

SA508 Gr.4N Ni-Cr-Mo low alloy steel, in which Ni and Cr contents are higher than in commercial SA508 Gr.3 Mn-Mo-Ni low alloy steels, may be a candidate reactor pressure vessel (RPV) material with higher strength and toughness from its tempered martensitic microstructure. The inner surface of the RPV is weld-cladded with stainless steels to prevent corrosion. The goal of this study is to evaluate the microstructural properties of the clad interface between Ni-Cr-Mo low alloy steel and stainless weldment, and the effects of post weld heat treatment (PWHT) on the properties. The properties of the clad interface were compared with those of commercial Mn-Mo-Ni low alloy steel. Multi-layer welding of model alloys with ER308L and ER309L stainless steel by the SAW method was performed, and then PWHT was conducted at $610^{\circ}C$ for 30 h. The microstructural changes of the clad interface were analyzed using OM, SEM and TEM, and micro-Vickers hardness tests were performed. Before PWHT, the heat affected zone (HAZ) showed higher hardness than base and weld metals due to formation of martensite after welding in both steels. In addition, the hardness of the HAZ in Ni-Cr-Mo low alloy steel was higher than that in Mn-Mo-Ni low alloy steel due to a comparatively high martensite fraction. The hardness of the HAZ decreased after PWHT in both steels, but the dark region was formed near the fusion line in which the hardness was locally high. In the case of Mn-Mo-Ni low alloy steel, formation of fine Cr-carbides in the weld region near the fusion line by diffusion of C from the base metal resulted in locally high hardness in the dark region. However, the precipitates of the region in the Ni-Cr-Mo low alloy steel were similar to that in the base metal, and the hardness in the region was not greatly different from that in the base metal.

• Economic and Environmental Geology
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• v.55 no.1
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• pp.29-43
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• 2022

The groundwater quality in Boseong area was characterized and their background concentrations were estimated based on the chemical data obtained from 200 groundwater samples collected during July 2019. Groundwater data were classified into two groups: Group 1 with NO₃^- < 44.3 mg/L and Group 2 with NO₃^- ≥ 44.3 mg/L. Results of t-tests indicate that groundwaters in Group 2 are significantly higher in water temperature and Ca concentration and significantly lower in F, As, Pb and Zn concentrations. It was also revealed that groundwaters Group 2 are closely linked to low pH, alkalinity, NH₄, NO₂, F, As, Fe, Mn, Pb and Zn levels, and high EC and water temperature. Background concentrations were estimated using the the BRIDGE method. The results depicted the higher levels in NO₃, NH₄, PO₄, As, Fe, Mn than the ones estimated by MOE and KECO (2018; 2019), which were prepared for the three catchment units in Boseong. The results of this study are believed to have more reliability because more data were used.

• Analytical Science and Technology
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• v.28 no.6
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• pp.436-445
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• 2015

Amomi Fructus with anti-oxidative activity was chosen and essential oil was obtained by SDE (simultaneous distillation extraction), and 39 constituents were determined by GC-MS (gas chromatography-mass spectrometry). Major components were camphor, borneol acetate, borneol, D-limonene and camphene. Three solvent extracts such as hexanes, diethyl ether and methylene chloride from Amomi Fructus were obtained. These were analyzed by GC-MS and 4 more constituents were identified in addition to 39 components discovered in essential oil. Five major components such as camphor, borneol acetate, borneol, D-limonene and camphene were also detected, however the relative peak percents of those components were different from those of constituents in essential oil. To estimate the kind and the amount of materials evaporated at certain temperature and conditions from essential oil and solvent extracts, dynamic headspace apparatus was used and materials evaporated and trapped at certain conditions were analyzed by GC-MS. Recovery yield of SDE method from Amomi Fructus was measured by using camphor and standard calibration solution of camphor methanol solution and, the yield was 82.0%. Content of Hg was measured by mercury analyzer and contents of Cd, Pb, Cr, Mn, Co, Ni, Cu and Zn in Amomi Fructus, essential oils and solvent extracts were determined by ICP-MS (Inductively coupled plasma-mass spectrometer). Pb, Cd and Hg were measured in the concentration of 0.72 mg/kg, <0.10 mg/kg and 0.0023 mg/kg, respectively and these were below permission level of purity test. Contents of Mn, Cu and Zn in Amomi Fructus were 213 mg/kg, 8.29 mg/kg and 31.0 mg/kg, respectively and which were relatively higher than other metals such as Cr, Co and Ni. Metals such as Mn (0.65 ~ 9.08 mg/kg), Cu (1.16 ~ 4.40 mg/kg) and Zn (1.10 ~ 3.80 mg/kg) in essential oil and solvent extracts were detected. At this point it is not clear that the metals were cross-contaminated in the course of treating Amomi Fructus or metals were contained in Amomi Fructus. The influence evaluation toward biological model study of these metals in essential oil and solvent extracts will be needed.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.217-225
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• 2005

This study was carried out to investigate the genotoxicity in comet and in vitro micronucleus assay and mutagenicity in Ames test of the extracts from leaves and stem of Morus alba L. The samples showed a very weak cytotoxicity on the NIH/3T3 cells by SRB assay. The cell viability of the extracts and fractions from leaves and stems of Morus alba L. was 80% over at $500\;{\mu}g/ml$, and that of the chloroform fractions from leaves and stems showed lower than others. The genotoxicity at $250\;{\mu}g/ml$ of 100% EtOH and water extracts on the NIH/3T3 cells in comet assay was about 40% compared to positive control, and most fractions from 100% EtOH extract of the leaves showed stronger genotoxicity than that offractions from the stem. The genotoxicity with S-9 mix in vitro micronucleus assay of the 100% EtOH and water extracts form Morus alba L. did not indicate any significant difference as compared with control group. The cytokinesis-binucleated cells were showed in the hexan, chloroform, ethylacetate and butanol fractions from the extract of the leaves without S-9, and sample with S-9 showed CB cells in the chloroform fraction from the leaves. In the Ames test, the water and 100% ethanol extracts of Morus alba L. did not have a strong mutagenicity in TA98 and TA100, but the fractions of organic solvents of the ethanol extract had $10{\sim}26%$ of mutagenicity on the TA100 strain.

• Journal of the Korean Geosynthetics Society
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• v.14 no.3
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• pp.31-42
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• 2015

The Reinforced subgrade for railroad (RSR) was constructed for one way railway line with the dimension of 5 m high, 6 m wide and 20 m long to evaluate its performance under train design load. The RSR has characteristics of short length (0.3-0.4 H) of reinforcement and rigid wall, 30 and 40 cm vertical spacing of reinforcement installation. To enhance economics and constructability, three kinds of connections (welding, hinge & bolt, bold wire) were also designed to realize the integration between rigid wall and reinforced subgrade. Two times of static loading tests were done on the full size railroad subgrade. The maximum applied pressure was 0.98 MPa (the maximum test load 5.88 MN), which corresponds to 19.6 times of the design load for railroad subgrade, 50 kPa. The performance on the RSR was evaluated with the safety on the failure, subgrade bearing capacity and settlement, horizontal displacement of wall, and reinforcement strain. Based on the full scale test, we confirmed that the RSR with the conditions of 0.35 H (35% of height) short reinforcement length, hinge & bolt type connection for integration between rigid wall and reinforced subgrade, and 40cm vertical spacing of reinforcement installment shows good performance under train design load.

• Journal of environmental and Sanitary engineering
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• v.12 no.3
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• pp.127-130
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• 1997

The removal of algae was conducted by loess, aluminum sulfate and PAC on a laboratory scale. The loess was consists of organic matter 1.4%, T-N $289{\mu}g/g$, T-P $17{\mu}g/g$, $Al841.2{\mu} g/g$, Fe $592.7{\mu}g/g$, Ca $10.6{\mu}g/g$, Mg $85.5{\mu}g/g$ and Mn $6.6{\mu}g/g$. Test water was dominated by Microcystis aeruginosa. When test water was mixed with 0.01, 0.05. and 0.1 g/$\ell$ of the loess in 5 minutes, after settled in 1 hour, the removal of chl-a was 2, 22, and 36% respectively. The removal of chl-a was 69%, 70% in pH 4.5 and 9.0 and above 92% between pH 5.0 and 8.0 after the $2mg/{\ell}$ of aluminum sulfate was added. When the $2mg/{\ell}$ of PAC and $0.05g/{\ell}$ of loess were added to test water together, the removal of chl-a was 95-99% in pH 4.0-8.0, 60% in pH 9.0, and 18% in pH 10.0. The removal of chl-a was higher when loess, aluminum sulfate and PAC was used together than used alone.

• Biomolecules & Therapeutics
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• v.15 no.3
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• pp.199-204
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• 2007

Carbamates have excellent insecticidal activities against a broad spectrum of insects. They possess knocking-down, fast-killing, and systemic effects, however, they are toxic to mammals. In this study, we have carried out in vitro genetic toxicity test of methylcarbamate and microarray analysis of differentially expressed genes in response to methylcarbamate. Methylcarbamate did not show mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. Methylcarbamate did not show mutations in frame shift TA98 both with and without exogenous metabolic activation. Methylcarbamate showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. Methylcarbamate did not increase micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to methylcarbamate selected differentially expressed 132 genes that could be candidate biomarkers of genetic toxic action of methylcarbamate.

• Biomolecules & Therapeutics
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• v.14 no.4
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• pp.246-252
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• 2006

1,2-Dibromoethane(DBE) has been widely used as a soil fumigant, an additive to leaded gasoline and an industrial solvent. In this study, we have carried out in vitro genetic toxicity test of 1,2-dibromoethane and microarray analysis of differentially expressed genes in response to 1,2-dibromoethane. 1,2-Dibromoethane showed mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed mutations in frame shift TA98 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. 1,2-Dibromoethane increased micronuclei in CRO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to 1,2-dibromoethane selected differentially expressed 241 genes that would be candidate biomarkers of genetic toxic action of 1,2-dibromoethane.

• Journal of Korean Society of Water and Wastewater
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• v.37 no.4
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• pp.187-202
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• 2023

This study focuses on the application of a new measurement method that quantifies the residual color of filtered water using a spectrocolorimeter after filtering the discolored substances. It was confirmed through the color and turbidity cross-test that the discolored substances cannot be measured effectively with the current legal color and turbidity test method. Therefore, the National Institute of Environmental Research's filter testing method, which involves filtering the sample through 0.45 ㎛ filter and visually inspecting the color, was improved. A membrane filter colorimetry (MFC) method was established by measuring the color difference (ΔE*ab(65)) of the filtered filter using a spectrophotometer and expressing it as filter color unit (FCU). Using the MFC method, the FCU for reference materials such as iron and manganese, as well as field samples, was measured. The results showed a high correlation with turbidity, and the color difference patterns varied depending on the type of reference materials and field samples. This indicates that the MFC method is an effective new measurement method of discolored tap water.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.72-72
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• 2018

A dynamic fatigue characteristic of dental implant system has been evaluated with applying single axial compressive shear loading based on the ISO 14801 standard. For the advanced dynamic fatigue test, multi-directional force and motion needed to be accompanied for more information of mechanical properties as based on mastication in oral environment. In this study, we have prepared loading and motion protocol for the multi-directional fatigue test of dental implant system with single (Apical/Occlusal; AO), and additional mastication motion (Lingual/Facial; LF, Mesial/Distal; MD). As following the prepared protocol (with modification of ISO 14801), fatigue test was conducted to verify the worst case results for the development of highly stabilized dental implant system. Mechanical testing was performed using an universal testing machine (MTS Bionix 858, MN, USA) for static compression and single directional loading fatigue, while the multi-directional loading was performed with joint simulator (ADL-Force 5, MA, USA) under load control. Basically, all mechanical test was performed according to the ISO 14801:2016 standard. Static compression test was performed to identify the maximum fracture force with loading speed of 1.0 mm/min. A dynamic fatigue test was performed with 40 % value of maximum fracture force and 5 Hz loading frequency. A single directional fatigue test was performed with only apical/occlusal (AO) force application, while multi directional fatigue tests were applied $2^{\circ}$ of facial/lingual (FL) or mesial/distal (MD) movement. Fatigue failure cycles were entirely different between applying single-directional loading and multi-directional loading. As a comparison of these loading factor, the failure cycle was around 5 times lower than single-directional loading while applied multi-directional loading. Also, the displacement change with accumulated multi-directional fatigue cycles was higher than that of single directional cycles.