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Genetic Toxicity Test of 1,2-Dibromoethane by Ames, Micronucleus, Comet Assays and Microarray Analysis  

Kim, Ki-Y. (College of Pharmacy, Ewha Womans University)
Kim, Ji-H. (College of Pharmacy, Ewha Womans University)
Kwon, Kyoung-J. (College of Pharmacy, Ewha Womans University)
Go, Seo-Y. (College of Pharmacy, Ewha Womans University)
Min, Kyung-N. (College of Pharmacy, Ewha Womans University)
Lee, Woo-S. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Park, Sue-N. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
Shee, Yhun-Y. (College of Pharmacy, Ewha Womans University)
Publication Information
Biomolecules & Therapeutics / v.14, no.4, 2006 , pp. 246-252 More about this Journal
Abstract
1,2-Dibromoethane(DBE) has been widely used as a soil fumigant, an additive to leaded gasoline and an industrial solvent. In this study, we have carried out in vitro genetic toxicity test of 1,2-dibromoethane and microarray analysis of differentially expressed genes in response to 1,2-dibromoethane. 1,2-Dibromoethane showed mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed mutations in frame shift TA98 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed DNA damage based on single cell gel/comet assay in L5178Y cells both with and without exogenous metabolic activation. 1,2-Dibromoethane increased micronuclei in CRO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in response to 1,2-dibromoethane selected differentially expressed 241 genes that would be candidate biomarkers of genetic toxic action of 1,2-dibromoethane.
Keywords
1,2-Dibromoethane; Ames test; COMET; MN assay; Microarray; S9 fraction;
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