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Antimicrobial Activity of Streptococcus mutans by Schizandrae fructus and Evodiae fructus extracts (오미자, 오수유 추출물의 Streptococcus mutans에 대한 항균효과)

  • Jeon, Eun-Sook;Han, Man-Deuk;Kim, Hyun-Day
    • Journal of dental hygiene science
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    • v.3 no.1
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    • pp.39-44
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    • 2003
  • This study was to investigate the antibacterial effect of Schizandrae fructus and Evodiae fructus extracts on S. mutans growth. The antibacterial effect of these extracts on S. mutans was good at Schizandrae fructus extract than the Evodiae fructus extract. The antibacterial activities of these extracts were measured 8.5 mm and 6.5 mm at 20 mg/ml concentration, respectively. The growth of S. mutans in control medium was the highest at 8 hrs, while the media of Schizandrae fructus and Evodiae fructus extracts added-medium(2 mg/ml) showed maximum growth at 16hrs. The pH values of the control medium was 5.08 at 8 hrs, but the media supplemented with Schizandrae fructus and Evodiae fructus extracts were 6.00, and 5.36 at 8 hrs, respectively. The amounts of total carbohydrate for the control media was 0.81 mg/ml at 8 hrs, but the media supplemented with Schizandrae and Evodiae fructus extracts were 1.32 mg/ml and 1.88 mg/ml at 8 hrs, respectively. In the change of culture medium proteins, the control culture broth and cultures supplemented with Schizandrae fructus and Evodiae fructus extracts were 8.39 mg/ml, 8.59 mg/ml and 9.97 mg/ml at 8 hrs, respectively. The S. mutans polysaccharide contents of the control medium and the media supplemented with Schizandrae and Evodiae fructus extracts were 300 mg/100 ml, 240 mg/100 ml and 280 mg/100 ml at 8 hrs, respectively.

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Study on the Status of Beverage Consumption of Middle and High School Students (중고생학생의 음료 섭취 실태에 관한 연구 -대전지역을 중심으로-)

  • 김복자
    • Journal of the Korean Home Economics Association
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    • v.27 no.3
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    • pp.79-87
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    • 1989
  • As the level of life improves, the pattern of beverage comsumption are being changed. We came to drink more beverage than ever before, and I think, the remarkable increase of beverage consumption will have a great effect on our health. We examined the drinking pattern of 447 middle school and high school students in Taejeon City from 24 to 29, May, 1989. We studied about the amount of drinking beverage and the correlation between the amount and the various factors to have effects on the amount. They usually drink total 604.6ml/day, water 292.8ml, soft drink 131.8ml, milk 109.2ml, fruit juice 26.1ml, vegetable juice 9.6ml, coffee & tea 21.7ml, korean tea 15.4ml. Beside, middle school students drink 654.4ml and high school students drink 560.7ml. The male drink 620.1ml and the female drink 589.2ml. The average amount of beverage consumption per a unit weight is total 13.3ml and middle school students (654.3ml) drink more than high school students (560.7ml) but the average amount is same between the male and the female. They usually drink 343.5ml of beverage at their homes, and they drink more at their homes than away from homes. In addition, at the correlation between the amount of drinking beverage and the various factor to have effects on the amount, the students who have snacks very often drink more than those who don't. And the students who like meat-diet drink much more than meat & vagetabe-diet persons and vegetorians. And the students who eat pread often drink more than those who have rice at every mealtime.

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Antimicrobial activities of oral bacteria by lichen extracts (지의류 추출물의 구강세균에 대한 항균효과)

  • Kim, Eun-Mi;Cho, Min-Jeong
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.1
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    • pp.81-91
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    • 2012
  • Objectives : By investigating antimicrobial activity of natural extracts, identifying its usefulness as antibiotic material to oral bacteria. Methods : Antimicrobial activity tests of 25 natural extracts were implemented on 6 type strains alleged to cause dental caries and 10 clinical strains isolated and identified from dental caries. Results : Among medicinal plants, Coptis japonica, Scutellaria baicalensis, Dictamnus dasycarpus showed antimicrobial activity. Among lichens, 3 methanol extracts and 6 acetone extracts showed antimicrobial activity. Minimum Inhibitory Concentration(MIC) test of Usnea aurantiacoatra KoLRI 004184, an acetone extract of lichen with the highest antimicrobial activity, on 6 type strains involved in dental caries and 10 strains isolated from dental caries resulted as follows; Actinomyces oris 1041 $12{\mu}g/ml$, for Corynebacterium durum 3151 $13{\mu}g/ml$, for Rothia dentocariosa KCTC $3204^T$ $14{\mu}g/ml$, for R. dentocariosa 911 $15{\mu}g/ml$, for R. dentocariosa 1822 $12{\mu}g/ml$, for Lacto-bacillus casei KACC $12413^T$ $12{\mu}g/ml$, for L. acidophilus KACC $12419^T$ $16{\mu}g/ml$, for L. rhamnosus 2421 $15{\mu}g/ml$, for Streptococcus mutans KCTC $3065^T$ $15{\mu}g/ml$, for S. mutans 121 $13{\mu}g/ml$, for Streptococcus oralis 2221 $18{\mu}g/ml$, for S. salivarius KCTC 5512 $14{\mu}g/ml$, for S. salivarius 122 $12{\mu}g/ml$, for S. sanguinis KCTC $3284^T$ $14{\mu}g/ml$, for S. sanguinis 912 $14{\mu}g/ml$, for Neisseria sp. KEM232 $12{\mu}g/ml$. Conclusions : Even a small amount of extract from lichen Usnea aurantiacoatra KoLRI 004184 showed very high level of antimicrobial activation against all 16 bacterial strains involved in dental caries.

Cytotoxic Effect of Korean Traditional Prescriptions on the Human Gastric Cancer Cell Lines (한약처방제의 인체 위암 세포주에 대한 세포독성 효과에 관한 연구)

  • Kim, Eun-Hae;Eun, Young-Ah;Kang, Bong-Joo;Sung, Hyun-Jae;Park, Kap-Joo
    • Korean Journal of Pharmacognosy
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    • v.28 no.4
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    • pp.233-238
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    • 1997
  • ln order to search for antigastric cancer agents from Korean traditional prescriptions. We selected 41 traditional prescriptions, based on a review of the Korean traditional medicine books. Both boiling water and methanol extracts were tested, by means of the Sulforhodamine B (SRB) protein assay. Six of the 41 water extracts; #3, #34, #35, #38, #40, #41 showed efficacy against gastric cancer cell (AGS: Human gastric carcinoma, ATCC HTB 103). #3 inhibited 50% cancer cell growth1 at the concentration of $152\;{\mu}g/ml$, #34, #35, #38, #40 and #41 inhibited 50% cancer cell growth at the concentration of $145\;{\mu}g/ml$, $129\;{\mu}g/ml$, $173\;{\mu}g/ml$, $10\;{\mu}g/ml$ and $19\;{\mu}g/ml$ respectively. Ten of the 41 methanol extracts; #1, #3, #32, #33, #35, #36, #37, #38, #41 were active. #1 inhibited 50% cancer cell growth at the concentration of $206\;{\mu}g/ml$, #3, #32, #33, #35, #36, #37, 738, #40, #41 inhibited 50% cancer cell growth at the concentration of $133\;{\mu}g/ml$, $159\;{\mu}g/ml$, $199\;{\mu}g/ml$, $147\;{\mu}g/ml$, $113\;{\mu}g/ml$, $187\;{\mu}g/ml$, $130\;{\mu}g/ml$, $9\;{\mu}g/ml$, $15\;{\mu}g/ml$ respectively. Prescription #3, #35, #38, #40, #41 were also interesting because both methanol and water extracts were active.

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Anti-metastastic Effects of ingredients of Fuzhengfangaitang (부정방암탕(扶正防癌湯) 구성(構成) 약물(藥物)의 암전이(癌轉移) 억제(抑制)에 대한 연구(硏究))

  • Park, Joon-Hyuck;Yu, Young-Beob;Shim, Bum-Sang;Choi, Seung-Hoon;Ahn, Koo-Seok
    • THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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    • v.7 no.1
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    • pp.39-60
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    • 2001
  • Fuzhengfangaitang is a prescript for inhibiting recurrence and metastasis of cancer. We had examined the anti-metatstastic effect of Fuzhengfangaitang. Furthermore, we performed the following experiments with ingredients of Fuzhengfangaitang. The purpose of this thesis is to study what ingredients of Fuzhengfangaitang have more valuable anti-cancer effects. And the results are listed below: 1. Cell Viability assay At the dose of 400$\mu$g/ml, most ingredients of Fuzhengfangaitang depressed viability of ECV-304. And especially, Scutellaria barbata D. DON$50{\mu}g/ml$ : 53.118%, $100{\mu}g/ml$: 49.092%, $200{\mu}g/ml$ : 43.765%, $400{\mu}g/ml$ : 12.747%), Polygonum bistorta L.($50{\mu}g/ml$ : 45.554%, $100{\mu}g/ml$ : 45.554%, $200{\mu}g/ml$ : 0.0%, $400{\mu}g/ml$ : 0.0%) and Psoralea corylifolia L.($50{\mu}g/ml$ : 86.591%, $100{\mu}g/ml$ : 81.307%, $200{\mu}g/ml$ : 24.801%, $400{\mu}g/ml$ : 3.111%) highly depressed cell viability more than the other ingredients. (${\alpha}$<0.05) 2. Cell Proliferation assay Proliferation assay with ingredients of Fuzhengfangaitang on ECV-304 showed that Crataegus pinnatifuda BGE ($50{\mu}g/ml$: 63.276%, $100{\mu}g/ml$ : 64.092%, $200{\mu}g/ml$ : 68.966% $400{\mu}g/ml$ : 38.517%, ED50=$296.974{\mu}g/ml$), Polygonum bistorta L.($50{\mu}g/ml$ : 83.981%, $100{\mu}g/ml$ : 86.997%, $200{\mu}g/ml$ : 58.780%, $400{\mu}g/ml$ : 26.408%), ED50=$266.725{\mu}g/ml$) and Psoralea corylifolia L.($50{\mu}g/ml$ : 103.037%, $100{\mu}g/ml$ : 82.529%, $200{\mu}g/ml$ : 2.829%, $400{\mu}g/ml$ : 0.998%), ED50=$177.369{\mu}g/ml$) depressed cell proliferation more than the other ingredients. 3. Tube Formation assay Compared with the control group, most ingredients of Fuzhengfangaitang did not remarkably inhibited the Tube Formation assay of ECV-304 at the dose of $100{\mu}g/ml$. But, Polygonum bistorta L. highly inhibited the tube formation of ECV -304 at the lower dose of $50{\mu}g/ml$. 4. Rat Aortic Ring assay In comparison with the control group, Scutellaria barbata D. DON., root of Polygonum bistorta L. and Psoralea corylifolia L. restricted the angiogenesis of the rat aortic ring at the dose of $100{\mu}g/ml$. And the other ingredients of Fuzhengfangaitang did not restricted the angiogenesis of the rat aortic ring at that dose. Especially, Polygonum bistorta L. highly inhibited the angiogenesis of the rat aortic ring at the lower dose of $50{\mu}g/ml$. From our research, the anti-angiogenic effects of the ingredients of Fuzhengfangaitang was proven. Moreover, it will be helpful for designing more effective prescription for anti angiogenesis.

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Sensitivity of Staphylococcus aureus Isolated From Bovine Udders to Various Chemotherapeutic Agents (유우유방원(乳牛乳房源) Staphylococcus aureus 의 각종 화학요법제(化學療法劑)에 대한 감수성시험(感受性試驗))

  • Han, H.R.;Chung, G.T.
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.85-89
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    • 1972
  • The minimum inhibitory concentration (MIC) of five chemotherapeutic agents (penicillin, streptomycin, tetracycline, oxytetracycline and furazolidone) was measured for 126 strains of Staphylococcus aureus isolated from the udder of dairy cattle. The results obtained were as follows: 1. The MIC of penicillin, streptomycin, tetracycline, oxytetracycline and furazolidone ranged from 0.03 to 32 ug/ml, 0.06 to 128 ug/ml, 0.06 to 128 ug/ml, 1.0 to 512 ug/ml, and 0.06 to 32 ug/ml, respectively. The most frequent MIC of the above drugs were; penicillin 0.5ug/ml, streptomycin 1.0ug/ml, tetracycline 0.5ug/ml, oxytetracycline 4.0ug/ml, and furazolidone 2.0ug/ml. 2. The number of strains resistant to penicillin. streptomycin, tetracycline and oxytetracycline were 89(70.6), 9(7.1%), 10(7.9%), and 26(20.6%), respectively. Twenty-eight (29.2%) strains showed multiple resistance to more than two antibiotics tested.

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A Design of XML DTD for Representation of the Internet Sheet Music (인터넷 악보 표현을 위한 XML DTD 설계)

  • 채진석
    • Journal of the Korean Society for information Management
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    • v.16 no.4
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    • pp.37-52
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    • 1999
  • In this paper, we propose a technique to represent a sheet music effectively on the Internet. For representing the Internet sheet music, this paper proposes the ScoreML(Score Markup Language) which is a new markup language based on XML, and describes the design of the XML DTD which defines the ScoreML. XML DTD constructing the ScoreML is designed to be able to represent various features of a sheet music in order to retrieve the real sheet music on the Internet. The sheet music built by the ScoreML is directly displayed on the Internet by ScoreML browser which is implemented in the form of Java applet.

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Bacterial Sanitation of Farm Water in Kyunggi Province (경기지역 목장수의 위생세균학적 분포)

  • 박석기;김성원;이강문;최성민;오영희
    • Journal of Environmental Health Sciences
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    • v.19 no.2
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    • pp.10-15
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    • 1993
  • Hygienic condition of farm waters has an effect on the hygiene of dairy foods. So we examined Standard Plate Count, coliform, E. coli, heat-resistant bacteria, psychrophilic bacteria, Pseudomonas aeruginosa and enterococcus for the bacterial sanitation of 78 farm waters in Kyunggi Province. Of the 78 farm waters, the average number of psychrophilic bacteria was 750 $\pm$ 170/ml, SPC 440 $\pm$ 130/ml, Pseudomonas aeruginosa 130 $\pm$ 97/100/ml, Coliform 22 $\pm$ 17/ml, E. coli 10 $\pm$ 6/100/ml, Heatresistant bacteria 5 $\pm$ 1ml, and Enterococcus 2 $\pm$ 1/100/ml. The percent of over than 1000/ml in SPC and psychrophilic bacteria of 78 farm water was 11.5% and 23.1%, respectively. The rate of over than 10/ml in coliform and heat-resistant bacteria was 12.8% and 15.4%, respectively and the rate of over than 10/100 ml in E. coli, Pseudomonas aeruginosa and enterococcus was 8.9%, 33.3%, and 2.6%, respectively. Pseudomonas aeruginosa was no significant between any other indicator organisms, and psychrophilic bacteria was significant with only SPC but other indicator organisms were highly significant with each other.

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Upper Bounds of Maximum Likelihood (ML) Decoding Performance of a few Irregular LDPC Codes (몇 개의 불규칙한 LDPC 부호의 Maximum Likelihood(ML) 복호에 대한 성능의 상향 한계와 정점 성능 감쇠 분석)

  • Chung, Kyu-Hyuk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.11C
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    • pp.1025-1028
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    • 2009
  • This paper presents upper bounds of Maximum Likelihood (ML) decoding performance of a few irregular LDPC codes using the simple bound and ML input output weight distributions and it is shown that contrary to general opinion that as block length becomes longer, BP decoding performance becomes simply closer to ML decoding performance, before peak degradation, as block length becomes longer, BP decoding performance falls behind ML decoding performance more and after peak degradation, general opinion holds.

Isolation and Identification of Lactic Acid Bacteria for Preparation of Probiotics (생균제용 유산균의 분리 및 동정)

  • 이진규;김운태;이준호;유재홍;신원철
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.429-432
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    • 1991
  • In order to prepare probiotics, a strain which has resistant against acid, bile salts and antibiotics was isolated from infant and anirnat feces. Among these isolated strains, TK- 19 showed a strong acid tolerance and growth at 500 $\mu g$/ml of Na-deoxycholate. Also strain TK-19 was resistant to kanarnycin (30 $\mu g$/ml), streptomycin (10 $\mu g$/ml), nisin (50 $\mu g$/ml) and tetracycline (10 $\mu g$/ml). The isolated strain TK-19 was identified as Streptococcus faeciam, and was named Streptococcus faeciurn TK-19.

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