• Journal of Animal Science and Technology
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• v.50 no.2
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• pp.177-184
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• 2008

Acid-labile subunit(ALS) is a 85-kDa glycosylated plasma protein which forms a 150-kDa ternary complex with 7.5-kDa insulin-like growth factor(IGF) and 40~45-kDa IGF-binding protein-3. In a previous study, the present authors prepared a porcine ALS(pALS) expression construct by inserting a pALS coding sequence into a plasmid vector following synthesis of the sequence by reverse transcription-polymerase chain reaction(RT-PCR). The expression construct, however, was subsequently found to have a mis-sense mutation at two bases of the pALS coding sequence which is presumed to have occurred through a PCR error. In the present study, the correct coding sequence was synthesized by the site-directed mutagenesis and inserted into the pET-28a(+) plasmid expression vector containing the His-tag sequence flanking the last codon of the insert DNA. After induction of the expression construct in E. coli BL21(DE3) cells, the resulting presumptive recombinant peptide was purified by the Ni-affinity chromatography. Upon SDS- PAGE, the affinity-purified peptide was resolved as a single band at a 66-kDa position which is consistent with the expected molecular mass of the presumptive recombinant pALS. Collectively, results indicate that a recombinant pALS peptide was successfully expressed and purified in the present study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.976-980
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• 2003

A strain of potential producer of fibrinolytic enzyme was isolated from Korean fermented food. The isolated bacterium was identified and named as Bacillus brevis KJ-23. The optimal condition of the medium for the production of fibrinolytic enzyme from Bacillus brevis KJ-23 was nutrient broth with 0.5% D-ribose, 0.5% malt extract and 0.3% $K_2$HPO$_4$. The optimum pH, temperature and fermentation time for the enzyme production were pH 7.0, 3$0^{\circ}C$ and 24 hr, respectively.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.1
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• pp.45-52
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• 2000

Nowadays, there are a lot of evidence that mutation of the p53 tumor suppressor gene is one of the most common genetic abnormalities in neoplastic progression. In this study, we analyzed 20 specimens of oral tumors(squamous cell carcinoma 14 cases, ameloblastoma 3 cases, adenoid cystic carcinoma 2 cases, malignant schwannoma 1 case)using polymerase chain reaction and direct sequencing which used an automated DNA sequencer and software for detection of mutations. Polymerase chain reactions were performed with 4 sets of primers encompassing exon 5, 6, 7, 8, and direct sequencing method was employed. The results were as followings. 1. We detected 10 point mutations out of 20 specimens (50%). 2. The genetic alterations included 7 mis-sense mutations resulting in single amino acid subtitutions, 2 silent mutations, 1 non-sense mutations encoding a stop codon. 3. Mutations were mostly in exon 7(7 out of 10 mutations, 70%) and involved codons 225, 234, 235, 236, 238, 247. 4. Therse were 4 cases of $T{\rightarrow}A$ transversion, 2 cases of $C{\rightarrow}A$ transversion, $A{\rightarrow}G$ transition, 1 case of $C{\rightarrow}G$, $T{\rightarrow}G$ transversion respectively. 5. We could find out point mutations more conveniently using PCR - Automated Direct Sequencing method.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.4
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• pp.645-651
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• 2006

This paper discusses computer system performance evaluation and analysis by employing a simulator which able to execute a symmetric multiprocessor in machine simulation environment. We also perform a multiprocessor system analysis using SPLASH-2, which is a suite of multi-program benchmarks for multiprocessors, to perform the behavior study of the symmetric multiprocessor OS kernel, IRIX5.3. To validate the scalability of symmetric multiprocessor system, we demonstrate structure and evaluation methods for symmetric multiprocessor as well as a functionality-based software simulator, SimOS. In this paper, we examine cache miss count and stall time on the symmetric multiprocessor between the local instruction and local data, using the multi-program benchmarks such as RADIX sorting algorithm and Cholesky factorization.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.6
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• pp.1151-1156
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• 2009

Unstable sustain discharges can occur at the bottom cells of the panel at high temperature. To solve this problem, the wall charge variation during an address period was investigated. A test panel of 7.5 inch XGA level was used and one green cell was measured. In order to realize operating condition equal to that of the bottom cells of 50 inch panel, the addressing stress pulses are applied. It seems that the resultant wall charge loss during address period increased with increase of stress time, temperature, pressure and Xe %. Wall charge loss increases with potential difference between scan electrode and address electrode, therefore wall charge loss can be minimized by the increase of scan voltage during address period.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.55 no.5
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• pp.248-253
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• 2006

The purpose of this study is to develop a portable gait-event detection system which is necessary for the cycle-to-cycle FES(functional electrical stimulation) control of locomotion. To make the system portable, we made following modifications in the gait signal measurement system. That is, 1) to make the system wireless using Bluetooth communication, 2) to make the system small-sized and battery-powered by using low power consumption ${\mu}$ P(ATmega8535L). The gait-events were analyzed in off-line at the main computer using ANN(Artificial Neural Network). The Proposed system showed no mis-detection of the gait-events of normal subject and hemiplegia subjects. The performance of the system was better than the previous wired-system.

• Proceedings of the KIEE Conference
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• 2005.07d
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• pp.2873-2875
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• 2005

The optimal structure of the conventional Fuzzy Polynomial Neural Networks (FPNN)[3] depends on experience of designer. For the conventional Fuzzy Polynomial Neural Networks, input variable number, number of input variable, number of Membership Functions(MFs) and consequence structures are selected through the experience of a model designer iteratively. In this paper, we propose the new design methodology to find the optimal structure of Fuzzy Polymomial Neural Network by using Genetic Algorithms(GAs)[4, 5]. In the sequel, It is shown that the proposed Advanced Genetic Algorithms based Fuzzy Polynomial Neural Network(Advanced GAs-based FPNN) is more useful and effective than the existing models for nonlinear process. We used Medical Imaging System(MIS)[6] data to evaluate the performance of the proposed model.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.5 no.1
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• pp.69-80
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• 2010

The purpose of this study is to help the companies that have the experience and know-how about ASP and that want to go abroad. This study analyzed information and communication infrastructure of Asia, foreign investment of Korean companies, and market attractiveness, selected China, Japan, Indonesia, and Malaysia as the target for the advance into overseas markets, and then suggested the strategy for ASP overseas markets. This study can contribute ASP-related venture company or entrepreneur.

• IE interfaces
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• v.13 no.2
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• pp.273-280
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• 2000

Time-tradeoff measures and healthy years equivalents were assessed and compared through an empirical study based on Multi-attribute utility theory. The study included 33 student subjects as a pilot study, and 54 end-stage renal disease patients in Wisconsin. The two outcome measures were compared with the survival duration of 1, 5, and 10 years. The results of the study show that the time-tradeoff method and the two-stage method did not lead to the same numerical quantities, although they aim to measure the same quantities (equivalent numbers of healthy years) theoretically. The healthy years equivalents involved more inconsistencies, and were less reliable than the time-tradeoff measures. Overestimation of the healthy years equivalents was observed. This seemed to be caused by the complex procedure of the two-stage method as well as by the preferences assessment biases. Based on the study experiences, the time-tradeoff measure would be recommended for problems involving generic medical applications and health policies.

• The Journal of the Korea Contents Association
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• v.5 no.5
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• pp.59-64
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• 2005

The biometrics of iris is a very accurate authentication method. The biometrics of iris can recognize and identify a person for shortly. But the image of iris is changed by transformation of body in the life. The existing iris authentication system has problem that can be mis-recognized. In this paper, we proposed and implemented Renewable Iris Authentication Algorithm(RIAA) for biometrics in authentication system. This algorithm tries to present a new way to people identification, we show contour line when shift take photograph in regular side. Namely, it generate iris code based on boundary of projection or submergence side and compared to original, so that it describes iris identification method to people identification.