• Tuberculosis and Respiratory Diseases
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• 제62권4호
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• pp.299-307
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• 2007

배경: HMGB1은 염증반응의 후기에 분비되는 중요한 염증유발물질 중 하나이다. 본 연구에서는 기존에 염증유발물질로 잘 알려진 LPS와 새롭게 염증유발물질로 관심을 받고 있는 HMGB1의 염증유발작용을 생체 외 및 생체 내 실험을 통해 비교하고자 하였다. 또한 HMGB1의 자극에 의한 IL-8 promoter region의 활성화에 중요한 역할을 수행하는 전사인자들을 확인하고자 하였다. 방법: RAW264.7 세포에 LPS(100 ng/ml) 또는 HMGB1(500 ng/ml)을 투여하고 각각 0, 2, 4, 8, 12 그리고 24시간 뒤에 세포상층액의 $TNF-{\alpha}$, MIP-2 그리고 $IL-1{\beta}$의 농도를 ELISA법으로 측정하였다. 생쥐의 복강에 LPS(5 mg/kg) 또는 HMGB1(2.5 mg/kg)을 주입하여 급성폐손상을 유발한 후에 폐의 사이토카인의 발현과 MPO 활성도를 측정하였다(LPS는 4시간 뒤, HMGB1은 24 시간 뒤). IL-8 promoter 부위에 있는 NF-IL6, $NF-{\kappa}B$ 그리고 AP-1에 대한 결합부위에 대해 돌연변이를 일으킨 후에 각각의 돌연변이체를 pIL-6luc에 결합시킨 뒤 RAW264.7 세포에 삽입하였다. 이 세포들을 36시간 배양한 후에 HMGB1(500 ng/ml)으로 자극하고, 한 시간 뒤에 세포를 녹인 후 luciferase 활성도를 측정하였다. 결과: LPS 투여 후에 RAW264.7 세포 배양상층액의 $TNF-{\alpha}$농도는 24시간 뒤에, MIP-2 농도는 8시간 뒤에 최고치를 보였다. 한편 HMGB1 투여 후에는 $TNF-{\alpha}$와 MIP-2 농도 모두 24시간 뒤에 최고치를 나타내었다. LPS 복강 내 투여 후 4시간 뒤에 생쥐의 폐의 $TNF-{\alpha}$, MIP-2 그리고 $IL-1{\beta}$의 농도는 대조군에 비해 현저히 증가하였으나, HMGB1 복강 내 투여 후 24시간 뒤에 생쥐의 폐에서는 $IL-1{\beta}$의 농도만 약간 증가하였다. MPO 활성도는 LPS와 HMGB1 투여 후에 모두 증가하였으며, LPS 투여 후가 더 의미있게 증가하였다. $NF-{\kappa}B$ 돌연변이체와 AP-1 돌연변이체에서 luciferase 활성도가 의미있게 감소하였다. 결론: 이상의 결과를 살펴볼 때 HMGB1은 염증유발효과는 LPS에 비해 강도가 떨어지나 지속시간은 오래 계속되는 것으로 보이며, HMGB1에 의한 IL-8의 활성화에 $NF-{\kappa}B$ 뿐만 아니라 AP-1도 중요한 역할을 수행하는 것으로 판단된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.816-817
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• 2007

As UNFCC(United Nations Framework Convention on Climate Change) is enhanced, Korea will perform a CO2 reduction duty. The CO2 reduction duty will effect Korea power system because coal and oil thermal generations emit large CO2 form about 46% of total CO2 emission. Moreover various alternatives should be designed to comply with CO2 reduction duty. In this paper, we analysis resource planning considering CO2 emission constraints and emission trading. And we analysis resource planning under using LP(Linear Programing) and MIP(Mixed Integer Programing).

• 한국정보통신설비학회:학술대회논문집
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• 한국정보통신설비학회 2008년도 정보통신설비 학술대회
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• pp.211-217
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• 2008

현재 무선 환경에서 이동성을 제공하려는 노력은 다양한 계층의 프로토콜에서 활발히 진행이 되고 있으며 이중에서도 IP 서브넷이 변경 되어도 이동성이 제공 가능한 IP 이동성 기술이 다양한 계층의 이동성 프로토콜 중에서도 가장 활발히 연구가 이루어지고 있다. IP 이동성 기술은 Mobile IP(MIP)가 나온 이후에 핸드오버 지연을 개선한 Hierarchical MIP 및 Fast MIP 등 다양한 타입으로 확장되어 연구가 진행 중에 있다. 그러나 MIP의 경우는 단말에 MIP Client 스택이 탑재 되어야 하고 단말의 전력 소모 및 HO 지연 크다는 단점 등으로 인하여 기술이 활성화되는데 한계성을 지녀 왔다. 따라서 최근에는 이를 개선한 Proxy MIP 관련 연구들이 활발히 이루어지고 있으며 또한 IP 계층 이동성 기술만으로는 성능 개선에 한계가 있다고 보고 다양한 계층의 이동성 기술과 연동을 하려는 시도도 동시에 이루어지고 있다. 따라서 본 논문에서는 타 계층에서 제공되는 MIH 프로토콜 기능을 활용하여 802.11 WLANs 환경에 Proxy MIPv4를 적용하여 이동성을 제공 시 발생하는 핸드오버 지연 요소를 최소화 하였다. 제안된 메커니즘은 MIH가 제공하는 Event, Command, Information 서비스를 활용하여 단말이 새로운 Target 망에 접속하기 이전에 인증 Key 교환 기법을 통해 핸드오버 시 발생하는 인증 지연을 최소화하였으며 추가적으로 Inter-AP간 Tunneling 및 Forwarding 기법을 적용하여 핸드오버 시 발생되는 Packet 손실을 최소화 하는 성능 향상 방안을 제안하였다.

• 한국통신학회논문지
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• 제28권12A호
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• pp.991-1001
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• 2003

CCFH(Candidatecasting Fast Handoff) 알고리듬은 기존의 Mobile IP에 멀티캐스팅을 결합한 고속 핸드오프의 성능을 능가하며, B/W 효율성 측면에서 역시 뛰어난 성능을 보이는 새로운 핸드오프 방법이다. 이 알고리듬은 L2 정보를 이용, L2 핸드오프가 발생하기 전 미리 멀티캐스팅하여 L3 핸드오프 지연을 타 방법에 비해 획기적으로 줄인다. 본 논문에서는 제안된 핸드오프 방법을 소개하고, 기존 MIP(Mobile IP)의 기본적인 핸드오프 방법뿐만 아니라 다른 핸드오프 방법들과의 비교 분석을 통하여 제안된 방법의 성능이 우수함을 확인한다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.929-935
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• 2014

Two types of nanosized titanium dioxide, anatase ($anTiO_2$) and rutile ($rnTiO_2$), are widely used in industry, commercial products and biosystems. $TiO_2$ has been evaluated as a Group 2B carcinogen. Previous reports indicated that $anTiO_2$ is less toxic than $rnTiO_2$, however, under ultraviolet irradiation $anTiO_2$ is more toxic than $rnTiO_2$ in vitro because of differences in their crystal structures. In the present study, we compared the in vivo and in vitro toxic effects induced by $anTiO_2$ and $rnTiO_2$. Female SD rats were treated with $500{\mu}g/ml$ of $anTiO_2$ or $rnTiO_2$ suspensions by intra-pulmonary spraying 8 times over a two week period. In the lung, treatment with $anTiO_2$ or $rnTiO_2$ increased alveolar macrophage numbers and levels of 8-hydroxydeoxyguanosine (8-OHdG); these increases tended to be lower in the $anTiO_2$ treated group compared to the $rnTiO_2$ treated group. Expression of $MIP1{\alpha}$ mRNA and protein in lung tissues treated with $anTiO_2$ and $rnTiO_2$ was also significantly up-regulated, with $MIP1{\alpha}$ mRNA and protein expression significantly lower in the $anTiO_2$ group than in the $rnTiO_2$ group. In cell culture of primary alveolar macrophages (PAM) treated with $anTiO_2$ and $rnTiO_2$, expression of $MIP1{\alpha}$ mRNA in the PAM and protein in the culture media was significantly higher than in control cultures. Similarly to the in vivo results, $MIP1{\alpha}$ mRNA and protein expression was significantly lower in the $anTiO_2$ treated cultures compared to the $rnTiO_2$ treated cultures. Furthermore, conditioned cell culture media from PAM cultures treated with $anTiO_2$ had less effect on A549 cell proliferation compared to conditioned media from cultures treated with $rnTiO_2$. However, no significant difference was found in the toxicological effects on cell viability of ultra violet irradiated $anTiO_2$ and $rnTiO_2$. In conclusion, our results indicate that $anTiO_2$ is less potent in induction of alveolar macrophage infiltration, 8-OHdG and $MIP1{\alpha}$ expression in the lung, and growth stimulation of A549 cells in vitro than $rnTiO_2$.

• Journal of Information Processing Systems
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• 제12권2호
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• pp.196-213
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• 2016

System Architecture Evolution (SAE) with Long Term Evolution (LTE) has been used as the key technology for the next generation mobile networks. To support mobility in the LTE/SAE-based mobile networks, the Proxy Mobile IPv6 (PMIP), in which the Mobile Access Gateway (MAG) of the PMIP is deployed at the Serving Gateway (S-GW) of LTE/SAE and the Local Mobility Anchor (LMA) of PMIP is employed at the PDN Gateway (P-GW) of LTE/SAE, is being considered. In the meantime, the Host Identity Protocol (HIP) and the Locator Identifier Separation Protocol (LISP) have recently been proposed with the identifier-locator separation principle, and they can be used for mobility management over the global-scale networks. In this paper, we discuss how to provide the inter-domain mobility management over PMIP-based LTE/SAE networks by investigating three possible scenarios: mobile IP with PMIP (denoted by MIP-PMIP-LTE/SAE), HIP with PMIP (denoted by HIP-PMIP-LTE/SAE), and LISP with PMIP (denoted by LISP-PMIP-LTE/SAE). For performance analysis of the candidate inter-domain mobility management schemes, we analyzed the traffic overhead at a central agent and the total transmission delay required for control and data packet delivery. From the numerical results, we can see that HIP-PMIP-LTE/SAE and LISP-PMIP-LTE/SAE are preferred to MIP-PMIP-LTE/SAE in terms of traffic overhead; whereas, LISP-PMIP-LTE/SAE is preferred to HIP-PMIP-LTE/SAE and MIP-PMIP-LTE/SAE in the viewpoint of total transmission delay.

• Journal of Yeungnam Medical Science
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• 제20권2호
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• pp.129-141
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• 2003

Background: Leptin is a 16-KDa non-glycosylated peptide hormone synthesized almost exclusively by adipocytes. The well-known function of leptin is regulation of food intake and energy expenditure. Leptin also plays a regulatory role in immune and inflammatory process including cytokine production. The purpose of this study was to investigate the effect of leptin on the expression of several chemokine genes(RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$) in THP-1 cells. Materials and Methods: Total RNA of THP-1 cells were prepared by Trizol method, and then stimulated with the leptin(250 ng/$m{\ell}$) or LPS(100 ng/$m{\ell}$). We examined the expression patterns of various chemokine mRNAs in THP-1 cell lines by RT-PCR and Northern blot. Results: Leptin did not induce the expression of chemokine mRNAs in THP-1 cells. The expression patterns of RANTES, IL-8, MCP-1, IP-10, and Mig mRNAs in THP-1 cells stimulated with leptin and LPS simultaneously was almost same to the patterns of LPS alone-induced chemokine mRNAs. RANTES mRNA expression was independent on the concentrations of leptin. Although leptin did not have strong effect on the expression of RANTES, IL-8, MCP-1, IP-10, Mig, MIP-$1{\alpha}$, MIP-$1{\beta}$, and GRO-${\alpha}$ mRNAs in THP-1 cells, leptin could induce the expression of long isoform of leptin receptor(OB-RL) mRNA, and its expression was elevated in simultaneous stimulation of leptin and LPS. Conclusion: These data suggest that leptin is able to induce OB-RL in THP-1 cells, however, leptin has little effect on the expression of pro-inflammatory chemokine genes.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2009년도 춘계 학술대회 제21권1호
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• pp.345-346
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• 2009

본 연구는 TG/DTA, MIP 장비를 활용한 콘크리트의 미세정량분석시, Ca(OH)$_2$량 및 미세공극구조 분석 결과값의 편차가 큰 것에 주목하여, 시료의 채취 위치가 분석결과에 영향을 미친다고 판단하여 시료의 채취 위치에 따른 미세정량분석 결과를 평가하였다. 그 결과 시료의 채취 위치에 따라 외부(P-1)에서 내부(P-3)일수록 Ca(OH)$_2$이 증가하며 공극량은 감소하는 것을 확인하였다.

• BMB Reports
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• 제43권4호
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• pp.257-262
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• 2010

The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 $\beta$ production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group II underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). Cells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group II, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 $\beta$ and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.

• 한국전문물리치료학회지
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• 제25권3호
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• pp.60-67
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• 2018

Background: Progressive muscle weakness is aggravated not only in the skeletal muscles but also in the respiratory muscles in many patients with neuromuscular diseases (NMD). Inspiratory muscle training (IMT) has been reported as therapy for pulmonary rehabilitation to improve respiratory strength, endurance, exercise capacity, and quality of life, and to reduce dyspnea. Objects: The purpose of this study was to determine the effect of playing harmonica for 5 months on pulmonary function by assessing the force vital capacity (FVC), peak cough flow (PCF), maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), and maximal voluntary ventilation (MVV) in patients with NMD. Methods: Six subjects with NMD participated in this study. The subjects played harmonica once a week for 2 hours at a harmonica academy and twice a week for 1 hour at home. Thus, training was performed thrice a week for 23 weeks. The examiner assessed pulmonary function by measuring FVC in the sitting and supine positions and PCF, MIP, MEP, and MVV in the sitting position at the beginning of training and once a month for 5 months. Results: Both sitting and supine FVC significantly increased after playing harmonica (p=.042), as did MIP (p=.043) and MEP (p=.042). Conclusion: Playing harmonica can be used as an effective method to improve pulmonary function in patients with NMD.