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http://dx.doi.org/10.5483/BMBRep.2010.43.4.257

Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus Scrofa

Klimenko, Oxana V. (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University)
Vobruba, Vaclav (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University)
Martasek, Pavel (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University)

Publication Information

BMB Reports / v.43, no.4, 2010 , pp. 257-262 More about this Journal

Abstract

The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 $\beta$ production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group II underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). Cells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group II, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 $\beta$ and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.

Keywords

BALF; CYP27A1; iNOS; MIP1 $\beta$ ; 7-ketcholesterol;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)
Times Cited By SCOPUS : 1

Reference

1	Colin, S., Bourguignon, E., Boullay, A. B., Tousaint, J. J., Huet, S., Caira, F., Staels, B., Lestavel, S., Lobaccaro, J. M. A. and Delerive, P. (2008) Intestine-specific regulation of PPAR alpha gene transcription by liver X receptors. Endocr. 149, 5128-5135 DOI ScienceOn
2	Lund, E. and Björkhem, I. (1997) Role of oxysterols in the regulation of cholesterol homeostasis: a critical evaluation. Acc. Chem. Res. 28, 241-249 DOI ScienceOn
3	Dzeletowic, S., Babiker, A. and Diczfalusy, U. (1995) Time course of oxysterols formation during in vivo oxidation of low density lipoprotein. Chem. Phys. Lipids. 78, 119 DOI ScienceOn
4	Antonchick, A. V., Zhabinskii, V. N. and Khripach, V. A. (2007) Oxysterols: genesis and basic functions. Russian J. Bioorganic. Chem. 33, 297-309
5	Cardenas, V. J. J. and Lynch, J. E. (2006) Mechanical ventilation and acute respiratory distress syndrome. Sem. Thoracic Cardiovasc. Sur. 18, 8-12 DOI ScienceOn
6	Webb, H. and Tierney, D. (1974) Experimental pulmonary edema due to intermittent positive pressure ventilation. Protection by positive end-expiratory pressure. Am. Rev. Respir. Dis. 110, 556-565 PUBMED
7	Syrkina, O., Jafari, B., Hales, C. A., and Quinn, D. A. (2008) Oxidant stress mediates inflammation and apoptosis in ventilator-induced lung injury. Respirology 13, 333-340 DOI ScienceOn
8	van den Bosch, H. M., Bunger, M., de Groot, P. J., vander Meijde, J., Hooiveld, G. J. E. and Muller, M. (2007) Gene expression of transporters and phase I/II metabolic enzymes in murine small intestine during fasting. BMC Genomics. 8, 267 DOI PUBMED
9	Pawar, A., Xu, J., Jerks, E., Mangelsdorf, D. J. and Jump, D. B. (2002) Fatty acid regulation of liver X receptors (LXR) and peroxisome proliferators-activated receptor $\alpha$ (PPAR $\alpha$ ) in HEK293 cells. J. Biol. Chem. 277, 39243-39250 DOI ScienceOn
10	European Convention for the protection of vertebrate animals used for the experimental and other scientific purposes (Strasbourg, 18.03.1986). Evropsk $\acute{a}$ dohoda o ochran $\check{e}$ obratlovcu pou $\check{z}$ ivan $\acute{y}$ ch pro pokusn $\acute{e}$ a jin $\acute{e}$ v $\acute{e}$ deck $\acute{e}$ $\acute{u}$ $\check{c}$ ely. (Sb. Mezin $\acute{a}$ rodnich smluv $\check{c}$ . 116/2003), 5634-5736
11	Babiker, A., Anderson, O., van der Linden, J. and Wiklund, B. (1999) Elimination of cholesterol as cholestenoic acid in human lung by sterol 27-hydroxylase: evidence that most of this steroid in the circulation is of pulmonary origin. J. Lipid Res. 40, 1417-1425 PUBMED
12	Pappolla, M. A., Bryant-Thomas, T., Petanceska, S., Perry, G., Thal, L. J., Sano, M. and Refolo, L. M. (2002) Cholesterol, oxidative stress, and Alzheimer’s disease: expanding the horizons of pathogenesis. Free Radic. Biol. Medic. 33, 173-181 DOI ScienceOn
13	Shan, H., Pang, J., Li, S., Chiang, T. B. and Schroepfer, G. J. J. (2003) Chromatographic behavior of oxygenated derivatives of cholesterol. Steroids 68, 221-233 DOI ScienceOn
14	Lemaire-Ewing, S., Prunet, C., Berthier, A., Corcos, L., Gambert, P., Neel, D. and Lizard, G. (2005) Comparison of the cytotoxic, pro-oxidant and pro-inflammatory characteristics of different oxysterols. Cell Biol. Toxicol. 21, 97-114 DOI ScienceOn
15	Volle, D. H., Mouzat, K., Duggavathi, R., Siddeek, B., Dechelotte, P., Sion, B., Veyssiere, G., Benahmed, M. and Lobaccaro, J. M. (2007) Multiple roles of nuclear receptors for oxysterols liver X receptor to maintain male fertility. Mol. Endocrin. 21, 1014-1027 DOI ScienceOn
16	Ferraz, T. P. L., Fiuza, M. C., Dos Santos, M. L., Pontes De Carvalho, L. H. and Soares, N. M. (2004) Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation. J. Biochem. Biophys. Meth. 58, 187-193 DOI ScienceOn
17	Joseph, S. B., Castrillo, A., Laffitte, B. A., Mangelsdorf, D. J. and Tontonoz, P. (2003) Reciprocal regulation of inflammation and lipid metabolism by liver X receptors. Nat. Med. 9, 213-219 DOI ScienceOn
18	Biasi, F., Leonarduzzi, G., Vizio, B., Zanetti, D., Sevanian, A., Sottero, B., Verde, V., Zingaro, B., Chiarpotto, E. and Poli, G. (2204) Oxysterol mixtures prevent proapoptotic effects of 7-ketocholesterol in macrophages: implications for proatherogenic gene modification. FASEB 18, 693-695
19	Okuda, M., Beard, M. R., Showalter, L. A., Scholle, F., Lemon, S. M. and Weinman, S. A. (2002) Mitochondrial injury, oxidative stress, and antioxidant gene expression are induced by hepatic C virus core protein. Gastroent. 122, 568-571 DOI PUBMED ScienceOn
20	Rodriguez, I. R. (2004) Rapid analysis of oxysterols by HPLC and UV spectroscopy. BioTechnique 36, 952-958
21	Kim, E. H., Lee, O. S., Joe, C. S. and Jou, I. (2006) Oxidized low density lipoprotein suppresses lipopolysaccharideinduced inflammatory response in microglia: Oxidative stress acts through control of inflammation. Biochem. Biophys. Res. Com. 342, 9-18 DOI ScienceOn
22	Folch, J., Lees, M. and Stanley, G. H. S. (1957) A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497-509 PUBMED
23	Prunet, C., Montage, T., Vejux, A., Rohmer, J. F. and Riedinger, J. M. A. (2006) Multiplexed flow cytometric analyses of pro- and anti-inflammatory cytokines in the culture media of oxysterol-treated human monocytic cells and in the sera of atherosclerotic patients. Cytometry 69, 359-373
24	Thommesen, L. and Laegreid, A. (2005) Distinct differences between TNF receptor-1 and TNF receptor 2-mediated activation of NFkB. J. BMB. 38, 281-289
25	Diestel, A., Aktas, O., Häke, I., and Raine, C. S. (2003) Activation of microglial poly(ADP-ribose)-polymerase-1 by cholesterol breakdown products during neuroinflammation:a link between demyelination and neuronal damage. J. Exp. Med. 198, 1729-1740 DOI ScienceOn
26	Lee, Ch. S., Joe, E. H. and Jou, J. (2006) Oxysterols suppress inducible nitric oxide synthase expression in lipopolysaccharide-stimulated astrocytes through liver X receptors. NeuroReport 17, 183-187 DOI ScienceOn
27	Deckert, V., Brunet, A., Lizard, G., Millanvoye van Brussel, E. and Monier, S. (1998) Inhibition by cholesterol oxides of NO release from human vascular endothelial cells. Arteriosclr. Tromb. Vasc. Biol. 18, 1054-1060 DOI ScienceOn
28	Schroepfer, G. J. J. (2000) Oxysterols: modulators of cholesterol metabolism and other processes. Physiol. Rev. 80, 361-554 DOI PUBMED
29	Keating, E., Rahman, L., Francis, J., Petersen, A., Possmayer, F., Veldhuizen, R. and Petersen, N. O. (2007) Effect of Cholesterol on the Biophysical and Physiological Properties of a Clinical Pulmonary Surfactant. Biophys. J. 93, 1391-1401 DOI ScienceOn
30	Frank, J. A., Imai, Yu. and Slutsky, A. S. (2003) Pathogenesis of ventilator-induced lung injury. in Acute respiratory distress syndrome, Matthay, M. A. (ed.), pp. 204-220, NY, Marcel Dekker, USA
31	Micheletta, F. and Luliano, L. (2006) Free radical attack on cholesterol: oxysterols as markers of oxidative stress and as bioactive molecules. Immun. Endocrine Met. Agents. 6, 305-316 DOI