• Journal of Korean Medicine for Obesity Research
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• v.9 no.2
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• pp.33-45
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• 2009

Objectives This study was performed to evaluate the relation between hair tissue mineral elements(HTME) and Heart rate variability(HRV) of the metabolic syndrome(MS). Methods 89 persons(41-69 ages) who visited Oriental hospital for medical examination were divided MS group(n=22) and control group(n=67). HTME and HRV were compared, and were analyzed correlation with five contents of the MS. Results (1) In total subjects, waist circumference had a positive correlation with Pb. Blood pressure had a negative correlation with Mg. High density lipoprotein cholesterol(HDL-chol) had a negative correlation with Pb, Sb and K, while positive correlation with Ca/K. Triglyceride(TG) had a negative correlation with Mg and Na/K, while positive correlation with K and Ca/Mg. (2) In total subjects, TG and fasting blood sugar(FBS) had negative correlation with high frequency(HF), while positive correlation with low frequency/high frequency ratio(LF/HF). (3) In MS group, most of the level of toxic minerals were higher, and the level of major nutritional minerals were lower, but there were no statistical signficance. In two groups, there is no contrast between the correlations of the MS contents and HTME. (4) In MS group, HF was significantly lower and LF/HF was higher than normal group. LF/HF had a negative correlation with waist circumference in MS group, while positive correlation in normal group. (5) In total subjects, TP had a positive correlation with Mg. In two groups, there is no contrast between the correlations of the MS contents and HRV. However LF/HF had a positive correlation with Na in MS group, TP and LF had negative correlation with Ca, while RMSSD and HF had negative correlation with Cu in normal group. Conclusion These results may suggest that HTME and HRV are useful in diagnosing and preventing the metabolic syndrome.

• Journal of Ginseng Research
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• v.24 no.3
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• pp.123-127
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• 2000

In this study, the effects of auxin and casein hydrolysate (CH) on the growth of ginseng hairy root was elucidated. Ginseng hairy root was cultured under light and dark conditions in MS solid and liquid medium with various concentrations of auxin and CH for fifty days. After harvesting the cultures, the fresh and dry weight of cultures were examined, respectively. In the MS solid culture, 1 mg/L of IBA was most effective on the growth of ginseng hairy root under the dark condition, whereas IAA and CH did not affect on the growth of ginseng hairy root. In the MS liquid culture, the growth was maintained regularly by the treatments of IAA and NAA. IBA and CH restrained the growth of ginseng hairy root.

• YAKHAK HOEJI
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• v.47 no.3
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• pp.142-147
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• 2003

Gas chromatography-mass spectrometric (GC-MS) procedure is presented for the simultaneous qualification and quantitation of methamphetamine (MA), amphetamine (AMP), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxyethylamphetamine (MDEA) in human hair. The method procedure involves decontamination of hair with distilled water and acetone, acidic hydrolysis, extraction in the presence of deuterated internal standards, and GC-MS analysis after derivatization with trifluoroacetic anhydride (TFAA) in ethylacetate. The limit of detection for 5 drugs were about 0.1∼0.15 ng/mg using 30 mg hair sample. Coefficient variations of correlation ranged from 0.9941 to 0.9993. The recoveries of these drugs were found to be 93.4∼104.4％. The concentrations of AMP, MA, MDA, and MDMA in abusers' hair samples were measured 0.17∼2.88, 2.09∼18.34, 0.24∼3.83, and 3.10∼22.81 ng/mg, respectively. The ratios of MA/AMP and MDMA/MDA ranged 5.67∼49.57 and 4.78∼54.31, respectively. This assay has been successfully utilized in the evaluation of the deposition of amphetamine-type stimulants (ATS) in human hair.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.330-336
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• 2020

Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.

• Journal of Food Hygiene and Safety
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• v.18 no.4
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• pp.195-201
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• 2003

An easily available, simultaneous identification/determination procedure for sildenafil, homosildenafil, tadalafil, vardenafil in adulterated health related foods was established by using a combination of three different analytical methods; thin layer chromatography(TLC), liquied chromatography-mass spectrometry (LC/MS) and high-performance liquied chromatography (HPLC)/photo-diode-array detector. The sample solution for TLC was applied to silica gel 60 $F_{254}$ plates with ethylacetate/acetonitrile/25%ammonia (90:10:5) as a developing solvent. Spots were located under UV radiation at 254 nm and dragendolfs reagent. Mass spectra of the compounds by LC/MS were investigated with electrospray ionization (ESI) interface, under positive ion mode. The HPLC analysis was performed on a column of capcell pack $C_{18}$ (UG120, 4.6${\times}$250mm I.D. 5 ${\mu}$m)with 0.1% sodium 1-hexansulfonate (in 0.1% phosphoric acid)/acetnitrile (73:27) as a mobile phase, and effluent was minitored with a photo-diode-applied to commercial foods, Sildenafil content was inthe range of 0.4mg/g~360.9 mg/g from 7 out of 35 samples. Homosildenafil content was in the range of 2.2 mg/g~336.0 mg/g from 7 out of 35 samples. Tadalafil content was 429.3 mg/g, 9.6 mg/500 mg from 2 out of 35 samples. The procedure described here is available for the screening of sildenafil, homosildenafil, tadalafil, vardenafil.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.41-45
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• 2002

An efficient and reproducible procedure for the large scale propagation of Hovenia dulcis Thunb. is described. Shoot primodia emerging from the leaf surface was induced from MS medium supplemented with NAA. Stem cuttings were suitable explants for multiple shoot proliferation. They produced axillary shoots which branched repeatedly, yielding an average of 7 shoots per explants after 4 weeks in culture, when cultured on a woody plant medium (WPM) containing 0.1mg/l BA and 0.1mg/l NAA. Stem, leaf and root segments from axenic seedlings were used as explant source to induce somatic embryogenesis. A high frequency of somatic embryos were induced directly from leaf in MS medium with NAA, 2,4-D and in medium containing NAA, 2,4-D with BA. Somatic embryos were germinated in MS medium supplemented with 1mg/ l $GA_3$. Somatic embryos proliferated secondary somatic embryos rapidly after transfer to MS medium supplemented with 1mg/ l kinetin, 1mg/ l $GA_3$ and 2% dextrose.

• Journal of Korean Society of Forest Science
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• v.79 no.3
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• pp.278-284
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• 1990

We have demonstrated expression of bacterial genes transferred into cells of Populus nigra ${\times}$ P. maximowiczii by A. tumefaciens strain 6044 (pGA 472). We determined the optimum concentration of kanamycin sulfate for effective selection of punctured leaf transformed using Agrobacterium binary vector pGA 472 containing a neomycine phosphotransferase gene (NPT-II) which confers kanamycin resistance. The combination of cefotaxime (200mg/l) and carbenicillin (300mg/l) showed good performance of discarding Agrobacterium from inoculated punctured leaf. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited callus and shoots induction from punctured leaf. Number of shoots regenerated from co-cultured punctured leaf was 3.0 on MS basal medium supplemented with 10 mg/l kanamycin sulfate, while that of not co-cultured punctured leaf was none. The regeneration rate was 10% from the punctured leaf co-cultured on MS medium with 10 mg/l kanamycin. Regenerated shoots are developing from micropropagation for Southern blot analysis and inheritance of the kanamycin resistance trait (NPT-II).

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.62-66
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• 2007

Plant Regeneration via organogenesis from leaf disk of Korean dandelion was investigated. Leaf disk cultured on MS medium with various combinations of BA (0-4 mg/L) and 2,4-D (0-1 mg/L). Shoot regeneration from leaf explant was observed after 3 weeks of culture. The highest shoot regeneration frequency from leaf disk was obtained with 2 mg/L BA. To analyze the effect of leaf age along shoot formation, we measured number of shoots per explant, shooting rate, fresh and dry weight of leaf explant. The highest number of shoots (11.5) per explant were obtained leaf from 7 weeks old plantlets after seed germination. The regenerated shoots were transferred in 1/2 MS medium with 0.5 mg/L NAA for root formation. Regeneratied plantlets thought organogenesis were growing to whole plants in the pots with acclimation.

• Culinary science and hospitality research
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• v.24 no.1
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• pp.63-72
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• 2018

The aim of this study was to investigate bioactive compounds from three domestic tomato varieties (Rafito, Momotaro TY Winner, and Medison). Lycopene, ${\beta}-carotene$ and polyphenols were quantified and identified using HPLC and LC-MS/MS. The levels of lycopene ranged from 28.36 mg/100 g to 60.18 mg/100 g. The content of ${\beta}-Carotene$ ranged from 2.00 mg/100 g to 2.92 mg/100 g. Ten kinds of polyphenol compounds were identified: caffeic acid-hexose isomer (I), caffeic acid-hexose isomer (II), 3-caffeoylquinic acid, 5-caffeoylquinic acid, caffeoylquinic acid isomer, quercetin-3-apiosylrutinoside, quercetin-3-rutinoside, di-caffeoylquinic acid, tri-caffeoylquinic acid, and naringenin chalcone. The level of 5-caffeoylquinic acid was the highest in domestic tomato varieties, ranging from 12.71 mg/100 g to 28.40 mg/100 g. The content of quercetin-3-rutinoside ranged from 3.74 mg/100 g to 17.64 mg/100 g. The contents of 3-caffeoylquinic acid and quercetin-3-apiosylrutinoside were 1.01~2.31 mg/100 g and 5.84~6.83 mg/100 g, respectively. Arrestingly naringenin chalcone was found only in Medison variety (36.82 mg/100 g). These results revealed that domestic tomato can be a good source of bioactive compounds for human health.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.269-272
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• 1997

Cotyledonary petioles of Brassica napus cocultivated with Agrobacterium vectors for 72 h were transferred to MS medium with 0.5 mg/L NAA, 2.0 mg/L BA, 30 mg/L kanamycin, 100 mg/L cefotaxime, 30 g/L sucrose, 3 mg/L $\textrm{AgNO}_{3}$ and 2 g/L Gelrite. The cotyledonary petioles with green shoots were selected at a frequency of 17.5% in a selection medium and then rooted. Southern blot analysis confirmed the rolC and NPT IIgenes were incorporated into the regenerated plants. The stable inheritance of rolC gene was confirmed in progeny test of transgenic plants.