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Simultaneous Identification/Determination System for Sildenafil, Homosildenafil, Vardenafil and Tadalafil as Adulterants in Foods  

Jang, Jae-Hee (Korea Food and Drug Administration)
Park, Kun-Sang (Korea Food and Drug Administration)
Park, Hye-Kyung (Korea Food and Drug Administration)
Koo, Yong-Eui (Korea Food and Drug Administration)
Choi, Youn-Ju (Korea Food and Drug Administration)
Hwang, In-Kyung (Seoul National University)
Kim, Dai-Byung (Seoul National University)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.4, 2003 , pp. 195-201 More about this Journal
Abstract
An easily available, simultaneous identification/determination procedure for sildenafil, homosildenafil, tadalafil, vardenafil in adulterated health related foods was established by using a combination of three different analytical methods; thin layer chromatography(TLC), liquied chromatography-mass spectrometry (LC/MS) and high-performance liquied chromatography (HPLC)/photo-diode-array detector. The sample solution for TLC was applied to silica gel 60 $F_{254}$ plates with ethylacetate/acetonitrile/25%ammonia (90:10:5) as a developing solvent. Spots were located under UV radiation at 254 nm and dragendolfs reagent. Mass spectra of the compounds by LC/MS were investigated with electrospray ionization (ESI) interface, under positive ion mode. The HPLC analysis was performed on a column of capcell pack $C_{18}$ (UG120, 4.6${\times}$250mm I.D. 5 ${\mu}$m)with 0.1% sodium 1-hexansulfonate (in 0.1% phosphoric acid)/acetnitrile (73:27) as a mobile phase, and effluent was minitored with a photo-diode-applied to commercial foods, Sildenafil content was inthe range of 0.4mg/g~360.9 mg/g from 7 out of 35 samples. Homosildenafil content was in the range of 2.2 mg/g~336.0 mg/g from 7 out of 35 samples. Tadalafil content was 429.3 mg/g, 9.6 mg/500 mg from 2 out of 35 samples. The procedure described here is available for the screening of sildenafil, homosildenafil, tadalafil, vardenafil.
Keywords
sildenafil; homosildenafil; tadalafil; vardenarfil; TLC; LC/MS; HPLC/PDA; health related food;
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