• Title/Summary/Keyword: M9 domain

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A Spatial-domain Fourier Transform Infrared Spectrometer: Application for Analyte Measurement in Cell Culture Media

  • Jung, Byung-Jo
    • Journal of the Optical Society of Korea
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    • 제9권4호
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    • pp.151-156
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    • 2005
  • A spatial-domain Fourier Transform (FT) infrared (IR) spectrometer coupled with a PtSi Schottky­barrier IR detector plane was developed in the spectral range of $2.0-2.5{\mu}m$ for noninvasive measurement of analyte concentrations in cell culture media during cell culture processing. A key optical component of the spectrometer is a Savart plate which is a birefringent polarizer generating coherent two rays for interfering. The spectral resolution of the spectrometer was determined as $71cm^{-1}$ (${\~}0.05{\mu}m$ at $2.5{\mu}m$). Clear IR fringe patterns were imaged on the IR detector plane. The feasibility of the spectrometer for our application was investigated by measuring absorbance spectra of glucose and fetal bovine serum (FBS) which are important compounds in cell culture media. Experiment results show that the spectral quality of glucose and FBS was comparable with the standard spectra acquired with a commercial FT-IR spectrometer, presenting the feasibility of the spectrometer to perform analyte measurement in cell culture media.

Initial Magnetization and Coercivity Mechanism in Amorphous TbxCo1-x Thin Films with Perpendicular Anisotropy

  • Kim, Tae-Wan;Lee, Ha-Na;Lee, Hyun-Yong;Lee, Kyoung-Il
    • Journal of Magnetics
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    • 제15권4호
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    • pp.169-172
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    • 2010
  • The coercivity mechanism in permanent magnets was analyzed according to the effects of domain nucleation and domain wall pinning. The coercivity mechanism of a TbCo thin film with high perpendicular magnetic anisotropy was considered in terms of the local inhomogeneity in the thin film. The initial magnetization curves of the TbCo thin films demonstrated domain wall pinning to be the main contributor to the coercivity mechanism than domain nucleation. Based on the coercivity model proposed by Kronmuller et al., the inhomogeneity size acting as a domain wall pinning site was determined. Using the measured values of perpendicular anisotropy constant ($K_u$), saturation magnetization ($M_s$), and coercivity ($H_c$), the inhomogeneity size estimated in a TbCo thin film with high coercivity was approximately 9 nm.

ON SURJECTIVITY OF m-ACCRETIVE OPERATORS IN BANACH SPACES

  • Han, Song-Ho;Kim, Myeong-Hwan;Park, Jong An.
    • 대한수학회보
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    • 제26권2호
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    • pp.203-209
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    • 1989
  • Recently many authors [2,3,5,6] proved the existence of zeros of accretive operators and estimated the range of m-accretive operators or compact perturbations of m-accretive operators more sharply. Their results could be obtained from differential equations in Banach spaces or iteration methods or Leray-Schauder degree theory. On the other hand Kirk and Schonberg [9] used the domain invariance theorem of Deimling [3] to prove some general minimum principles for continuous accretive operators. Kirk and Schonberg [10] also obtained the range of m-accretive operators (multi-valued and without any continuity assumption) and the implications of an equivalent boundary conditions. Their fundamental tool of proofs is based on a precise analysis of the orbit of resolvents of m-accretive operator at a specified point in its domain. In this paper we obtain a sufficient condition for m-accretive operators to have a zero. From this we derive Theorem 1 of Kirk and Schonberg [10] and some results of Morales [12, 13] and Torrejon[15]. And we further generalize Theorem 5 of Browder [1] by using Theorem 3 of Kirk and Schonberg [10].

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NMR Signal Assignments of Human Adenylate Kinase 1 (hAK1) and its R138A Mutant (hAK1R138A)

  • Kim, Gilhoon;Chang, Hwanbong;Won, Hoshik
    • 한국자기공명학회논문지
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    • 제20권2호
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    • pp.56-60
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    • 2016
  • Adenylate kinase (AK) enzyme which acts as the catalyst of reversible high energy phosphorylation reaction between ATP and AMP which associate with energetic metabolism and nucleic acid synthesis and signal transmission. This enzyme has three distinct domains: Core, AMP binding domain (AMPbd) and Lid domain (LID). The primary role of AMPbd and LID is associated with conformational changes due to flexibility of two domains. Three dimensional structure of human AK1 has not been confirmed and various mutation experiments have been done to determine the active sites. In this study, AK1R138A which is changed arginine[138] of LID domain with alanine[138] was made and conducted with NMR experiments, backbone dynamics analysis and mo-lecular docking dynamic simulation to find the cause of structural change and substrate binding site. Synthetic human muscle type adenylate kinase 1 (hAK1) and its mutant (AK1R138A) were re-combinded with E. coli and expressed in M9 cell. Expressed proteins were purified and finally gained at 0.520 mM hAK1 and 0.252 mM AK1R138A. Multinuclear multidimensional NMR experiments including HNCA, HN(CO)CA, were conducted for amino acid sequence analysis and signal assignments of $^1H-^{15}N$ HSQC spectrum. Our chemical shift perturbation data is shown LID domain residues and around alanine[138] and per-turbation value(0.22ppm) of valine[179] is consid-ered as inter-communication effect with LID domain and the structural change between hAK1 and AK1R138A.

마이크로스트립 패치 안테나의 다중 분해능 웨이블릿 산란해석법 (A Multiresolution Wavelet Scattering Analysis of Microstrip Patch antennas)

  • 강병용;주세훈;빈영부;김형훈;김형동
    • 한국전자파학회논문지
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    • 제9권5호
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    • pp.640-647
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    • 1998
  • 다중 분해능 웨이블릿 해석법을 마이크로스트립 패치 안테나의 산란해석에 적용하였다. 다충구조에 대한 스펙 트럼 영역 그린 함수(spectral domain Green's dyad)의 특성올 공간-스펙트럼 영역 표현법을 이용하여 살펴보고, 스펙트럼 영역 웨이블릿을 주어진 문제에 적용하는 것이 유용함을 관찰하였다. 적분방정식에 모멘트법을 이 용하여 행렬방정식을 얻고, 그 풀이에 CG(conjugate gradient)법과 스펙트럼 영역 웨이블릿올 결합하여 효율 적으로 문제를 풀이할 수 있다. 단충구조 위에 놓인 정방형 패치에 대하여 기폰의 모멘트법 결과와 다충 분해능 웨이블릿 해석법올 적용한 결과를 비교하였다.

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Microwave Dielectric Characterization of Binary Mixtures of 3-Nitrotoluene with Dimethylacetamide, Dimethylformamide and Dimethylsulphoxide

  • Chaudhari, Ajay;Chaudhari, H.C.;Mehrotra, S.C.
    • Bulletin of the Korean Chemical Society
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    • 제25권9호
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    • pp.1403-1407
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    • 2004
  • Dielectric relaxation measurements on 3-nitrotoluene (3-NT) mixture of dimethylacetamide (DMA), dimethylformamide (DMF) and dimethysulphoxide (DMSO) have been carried out across the entire concentration range using Time domain reflectometry technique at 15, 25, 35 and $45^{\circ}C$ over the frequency range from 10 MHz to 20 GHz. For all the mixtures, only one dielectric loss peak was observed in this frequency range and the relaxation in these mixtures can be well described by a single relaxation time using Debye model. Bilinear calibration method is used to obtain complex permittivity ${\varepsilon}^{*}({\omega})$ from complex reflection coefficient ${\rho}^{*}({\omega})$ over frequency range 10 MHz to 20 GHz. The excess permittivity, excess inverse relaxation time, Kirkwood correlation factor, molar energy of activation are also calculated for these mixtures to study the solute-solvent interaction.

Time Harmonic interactions in the axisymmetric behaviour of transversely isotropic thermoelastic solid using New M-CST

  • Lata, Parveen;Kaur, Harpreet
    • Coupled systems mechanics
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    • 제9권6호
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    • pp.521-538
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    • 2020
  • The present study is concerned with the thermoelastic interactions in a two dimensional homogeneous, transversely isotropic thermoelastic solid with new modified couple stress theory without energy dissipation and with two temperatures in frequency domain. The time harmonic sources and Hankel transform technique have been employed to find the general solution to the field equations.Concentrated normal force, normal force over the circular region, thermal point source and thermal source over the circular region have been taken to illustrate the application of the approach. The components of displacements, stress, couple stress and conductive temperature distribution are obtained in the transformed domain. The resulting quantities are obtained in the physical domain by using numerical inversion technique. Numerically simulated results are depicted graphically to show the effect of angular frequency on the resulted quantities.

대학생이 인식하는 학생성공 개념에 관한 탐색적 연구: CQR-M 분석을 중심으로 (An Exploratory Study on the Concept of Student Success Recognized by College Students)

  • 류소형;탁진국
    • 한국심리학회지 : 코칭
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    • 제5권1호
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    • pp.33-65
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    • 2021
  • 본 연구는 대학생이 인식하는 학생성공의 개념을 조사하기 위한 탐색적 연구이다. 학생들의 자유로운 생각을 듣기위해 온라인 설문지를 활용해 개방형 설문을 진행하고 99명의 응답을 분석하였다. 응답결과는 CQR-M(Consensual Qualitative Research-Modified)을 사용하여 분석하였다. 분석결과 '학생성공의 의미', '학생성공을 위한 대학 환경 요인', '학생성공에 필요한 심리적 요인' 등 3개의 영역에서 25개 범주가 도출되었다. 첫 번째 영역인 대학생이 인식하는 '학생성공의 의미'에서는 '학업성취·취업 역량 증진·다양한 경험 쌓기·만족스러운 취업·원하는 진로 설정·관계형성 능력 증진·나만의 목표 설정과 달성·자기 개발·만족스러운 대학생활'의 9개의 범주가 도출되었다. 두 번째 영역인 '학생성공을 위한 대학 환경 요인'에서는 '진로개발 지원·직무역량 향상 시스템·대학생활 참여지원·인적 네트워크 형성 기회·학습역량 증진 시스템·경제적 지원 확대·학생 편의를 위한 환경'으로 7개 범주가 도출되었다. 세 번째 영역인 '학생성공에 필요한 심리적 요인'에서는 '학습능력·자기효능감·대인관계 유능성·자기인식 및 개선·직무역량·자기조절 능력·풍부한 경험·진로인식·자존감'의 9개의 범주가 도출되었다. 각 영역별 빈도와 결과를 분석하여 제시하고, 연구의 의의와 제한점을 논의하였으며, 후속연구에 대한 제언을 하였다.

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지하유류저장시설 주변의 단열암반 내 지하수유동체계 해석

  • 조성일;김천수;배대석;김경수;박경우;송무영
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2005년도 총회 및 춘계학술발표회
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    • pp.292-295
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    • 2005
  • 본 연구는 지하유류저장공동 굴착 시 비교적 정밀하게 해석된 단열체계 및 수리인자를 토대로 투수성구조영역과 수리암반영역으로 세분화하여 연구지역의 불규칙하고 복잡한 지하수유동체계를 해석해 보고자 하였다. FZ-2 구조대와 인접한 수리암반영역 Domain-A와 B는 Domain-C와 D에 비해 수평수벽공의 초기압이 최대 약 $15kg/cm^2$정도 높으며, 상 하부의 수리적 연결성이 양호하여 지하공동굴착 시 상 하부의 수위차가 크지 않고 지하수 함양량은 약 $35{\sim}50mm/year$의 범위를 보인다. 또한 공동굴착 시 투수성 단열과의 교차에 의한 수위강하에 민감한 반응을 보이며 상 하부의 수위강하양상이 유사한 특성을 나타낸다. 반면, FZ-1 구조대와 인접한 Domain-C와 D는 지하공동 부근의 수리전도도가 각각 $7{\times}10^{-10},\;2{\times}10^{-9}m/sec$로 Domain-A와 B에 비해 최대 약 6배정도 낮고, 상 하부의 수리적 연결성이 양호하지 않기 때문에 공동굴착 전 이중수위측정시설 설치 시 계측된 상 하부의 수위차는 최대 약 120 m로 매우 크다. 그리고 상부의 지하수는 하부의 낮은 수리전도도로 인하여 수직방향보다 수평방향으로의 유동이 우세하며 공동굴착 시 수위변화는 크지 않고 함양량은 $10{\sim}15mm/year$의 범위를 나타낸다.

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