• Journal of Acupuncture Research
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• v.21 no.3
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• pp.107-119
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• 2004

Objective : The purpose of this study was to investigate the effect of Bee Venom and melittin on the lipopolysaccharide(LPS) and sodium nitroprusside(SNP)-induced expressions of Cell viability, nitric oxide(NO), inducible nitric oxide synthase(iNOS), extra-signal response kinase(ERK), jun N-terminal Kinase(JNK) and p38 kinase(p38)- mitogen activated protein kinase(MAPK) Family- in RAW 264.7 cells, a murine macrophage cell line. Methods : The expressions of cell viability by MTT assay, NO by Nitrite assay and iNOS, ERK, JNK and p38 were determined by Western blotting. Results : 1. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin increased cell viability of RAW 264.7 induced by LPS and SNP significantly respectively. 2. Compared with the control group, 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of NO induced by LPS and SNP significantly respectively. 3. Compared with the control group, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by LPS significantly and 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin inhibited expression of iNOS induced by SNP significantly. 4. Compared with the control group, the expression of ERK induced by LPS and SNP decreased significantly in the treatment groups of $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, which of p-ERK by LPS also did in 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin, but which of p-ERK by SNP did not decrease. 5. Compared with the control group, the. expression of JNK induced by LPS and SNP decreased significantly in the treatment groups of 5, $10{\mu}g/m{\ell}$ melittin, which of p-JNK by LPS in 5, $10{\mu}g/m{\ell}$ melittin and by SNP in $1{\mu}g/m{\ell}$ bee venom and $10{\mu}g/m{\ell}$ melittin decreased significantly. 6. Compared with the control group, the expression of p38 induced by LPS did not have significant difference, which induced by SNP decreased significantly in the treatment groups of 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin. p-p38 induced by LPS decreased significantly in the treatment group of $10{\mu}g/m{\ell}$ of melittin, which induced by SNP also decreased significantly in 0.5, 1, $5{\mu}g/m{\ell}$ bee venom and 5, $10{\mu}g/m{\ell}$ melittin.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.492-501
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• 2009

This study was carried out to investigate influence of tree height on vegetable growth, productivity, and labor input in 5 year old slender spindle 'Fuji'/M.9 apple trees planted with the tree space of $3.2{\times}1.2m$ and trained to the tree height of 2.5m in the apple orchard of Apple Research Station, NIHHS, RDA in Gunwi County, Korea. Tree height was extended to the tree height of 4.0m for two years. And then, the tree height was restricted to 2.5 (control), 3.0, 3.5, and 4.0m in 8th year. Yield per 10a was 46, 25, and 4% higher at the tree height of 4.0, 3.5, and 3.0m than at the tree height of 2.5m respectively in first year and 17, 12, and 10% higher respectively, indicating the taller tree, the higher yield. Soluble solid contents was highest in the height of 2.5m than in any other treatments for 2 years, but fruit skin color was not clear. Labor input was increased parallel with the increase of tree height, but yield per labor input was decreased. Gross income and net income per 10a were increased parallel with the increase of tree height in first year, but not increased in second year. In considering production, labor input, and economic efficiency, the optimum tree height of slender spindle 'Fuji'/M.9 apple planted with the tree space of $3.2{\times}1.2m$ was presumed to be 3.0~3.5m.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.993-1000
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• 2023

Recently, enviromentally friendly natural substances derived from plants have been attracting attention as cosmetic materials, and research on various physiological activities of natural substances is being actively conducted. This study investigated the antioxidant, anti-inflammatory, moisturizing, and antibacterial effects of three types of extracts of mixtures containing different mixing ratios, Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens, known to have various physiological activities. The mixing ratio is 7 conditions (M1, 1:1:1; M2, 0.5:1.5:1; M3, 1.5:0.5:1; M4, 0.1:0.95:0.95; M5, 0.5:0.5:2; M6, 0.95 :1.95:0.1; M7, 1.45:0.1:1.45), and the optimal mixing ratio was confirmed for use as a cosmetic material. DPPH and ABTS radical scavenging activities showed scavenging abilities of 75.37% and 99.19%, respectively, at 1,000 ㎍/mL of M6. At a concentration of 200 ㎍/mL of M6, it showed 50% of nitric oxide production inhibition compared to the lipopolysaccharide-treated that induced an inflammatory response. It was confirmed that M3 and M6 produced hyaluronic acid 1.47 and 1.49 times higher than the control at a concentration of 50 ㎍/mL, respectively. Through the disc diffiusion test, the clear zone was 9.75 mm at 8 ㎍/mL of M6, confirming the inhibition of growth of staplylococcus aureus strain. Based on the above results, it is believed that the mixed extract of Polygala tenuifolia, Angelica dahurica, and Elsholtzia splendens can be used as a functional natural material for cosmetics.

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.220-225
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• 1991

Activities of the $F_1-ATPase$ purified from Lentinus edodes were stimulated by $Fe^{3+},\;Fe^{2+},\;Cd^{2+},\;Mg^{2+},\;K^{+}\;and\;Co^{2+}$ but were inhibited by $Zn^{2+},\;Ca^{2+},\;Cu^{2+}\;and\;Ni{2+}$ ion. The enzyme activities were increased 130, 65, 65, 68, 105% and 23% by the 5mM $Fe^{3+}$, 10 mM$Fe^{2+}$, 1mM $Cd^{2+}$, 5mM $Mg^{2+}$, 5mM $K^{+}$ and 5mM$Co^{2+}$ ion addition, respectively, as compared with those not added. The enzyme activities were decreased 18, 19, 27 and 30% by 10 mM $Zn^{2+}$, 10mM $Ca^{2+}$, 0.5 mM $Cu^{2+}$ and 10 mM $Ni^{2+}$ ion, respectively. Anion effects of 10 mM ${Co_3}^{2-}$, 20 mM,$CN^{-}$ 20 mM$CH_3COO^{-}$ and 20 mM ${NO_3}^{-}$ ion were inhibited to the enzyme activities of 98, 95, 70 and 50%, respectively. As increasing of ${SO_4}^{2-}$ ion concentration, the enzyme activity was stimulated and 20 mM ${SO_4}^{2-}$ ion was shown increased of 21%.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.2
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• pp.122-128
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• 2005

The experiments were conducted to fine out the handling characteristics of the FMT(Frame Mideater Trawl) in the southern waters of Korea using a trawler "DONGBAEK" belongs to Yosu National University. The realtionship between the net depth D(m) and the warp length L(m) at the towing speeds of 2.5k't and 3.5k't werw as follows ; D(m) = 0.30L - 1.3(2.5k't), D(m) = 0.16L - 1.5(3.5k't). Therefore, the net depth was 3.0m deeper when the warp length was 10m longer at the towing speed of 2.5k't and was 1.6m deeper for 10m longer at the speed of 3.5k't, respectively. The sinking speed of FMT was 6.5m/min when the warp releasing speed was 24m/min at the towing speed of 2.5k't and was 3.8m/min for 25m/min at the towing speed of 3.5k't, respectively. The rising speed of FMT was 6.9m/min when the warp rewinding speed was 28m/min at the towing speed of 2.5k't and was 5.3m/min for 25m/min at the towing speed of 3.5k't, respectively. The mean elapsed time getting to the stable towing condition was 104sec at the towing speed of 2.5k't and was 105sec at the towing speed of 3.5k't, respectively, and there was no time difference for the towing speed variation. During the towing, the net depth was comparatively stable on the condition of no change for the warp length and the towing speed.

• Korean Journal of Environmental Agriculture
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• v.42 no.4
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• pp.389-395
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• 2023

This study evaluated the effects of gibberellic acid (GA₃) on the growth and quality of creeping bentgrass (Agrostis palustris Huds.). Experimental treatments included a No application of fertilizer and GA₃ (NFG) Control [3 N active ingredient (a.i.) g/m²], 0.3GA₃ (GA₃ 0.3 a.i. mg/m²/200 mL), 0.6GA₃ (GA₃ 0.6 a.i. mg/m²/200 mL), 1.2GA₃ (GA₃ 1.2 a.i. mg/m²/200 mL), and 2.4GA₃ (GA₃ 2.4 a.i. mg/m²/200 mL). Additionally, the study included a 1.5N+GA₃ experiment with similar GA₃ treatments combined with 1.5N a.i. g/m² : NFG, Control (3N a.i. g/m²), 1.5N+ 0.3GA₃ (1.5N a.i. g/m²+GA₃ 0.3 a.i. mg/m²/200 mL), 1.5N+0.6GA₃ (1.5N a.i. g/m²+GA₃ 0.6 a.i. mg/m²/200 mL), 1.5N+1.2GA₃ (1.5N a.i. g/m²+GA₃ 1.2 a.i. mg/m²/ 200 mL), and 1.5N+2.4GA₃ (1.5N a.i. g/m²+GA₃ 2.4 a.i. mg/m²/200 mL). Compared to the NFG, turf color index chlorophyll content was not significantly different (p< 0.05). However, shoot length in 1.2GA₃, 2.4GA₃, 1.5N+0.3GA₃, 1.5N+0.6GA₃, 1.5N+1.2GA₃, and 1.5N+2.4GA₃ treatments increased by 0.8%, 10.6%, 5.15%, 8.3%, 13.5 %, and 21.6%, respectively, compared to the control. As compared to the control, clipping yield in 1.5N+1.2GA₃ and 1.5N+2.4GA₃ treatments increased by 7.1% and 14.3 %, respectively. These results indicated that GA₃ application increased shoot length, with the 1.2GA₃ treatment showing shoot length similar to the control (3N a.i. g /m² ).

• Journal of Life Science
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• v.33 no.3
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• pp.227-233
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• 2023

The purpose of this paper was to describe a statistical analysis for the spatial distribution of geographical distances of Erythronium japonicum at Mt. Geumjeong in Korea. The spatial pattern of E. japonicum was analyzed according to the nearest neighbor rule, population aggregation under different plot sizes by dispersion indices, and spatial autocorrelation. Most natural plots of E. japonicum were uniformly distributed in the forest community. Disturbed plots were aggregately distributed within 5 m × 5 m of one another. Neighboring patches of E. japonicum were predominantly 7.5~10 m apart on average. If the natural populations of E. japonicum were disturbed by human activities, then the aggregation occurred in a shorter distance than the 7.5~10 m distance scale. The Morisita index (IM) is related to the patchiness index (PAI) that showed the 2.5 m × 5 m plot had an overly steep slope at the west and south areas when the area was smaller than 5 m × 5 m. When the patch size was one 2.5 m × 5 m quadrat at the west distributed area of Mt. Geumjeong, the cluster was determined by both species characteristics and environmental factors. The comparison of Moran's I values to a logistic regression indicated that individuals in E. japonicum populations at Mt. Geumjeong could be explained by isolation by distance.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.25-31
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• 1995

This study was carried out to investigate the effect of the concentration of nutrient solution on the growth of tomato(Lycopersicon esculentum Mill. cv. seokwang) in substrate culture. The substrates used in the experiment were perlite, vermiculite, and peatmoss. Tomato plants were treated with different concentrations of nutrient solution, viz. 0.5, 1.0, 2.0, 3.0, and 5.0mS/cm at seedling stage and transferred to different treatments, 1.0, 2.0, and 3.0mS/cm after transplanting in each substrate. As the concentrations of nutrient solution increased from 0.5 to 3.0mS/cm at seedling stage, the $CO_2$ assimilation rates of seedlings increased in all three substrate culture. Beyond this range, the $CO_2$ assimilation rates of seedlings decreased. By increasing the concentrations of nutrient solution, plant height, leaf length, leaf width, stem diameter, and top dry weight increased in perlite and were high at 2-5mS/cm in vermiculite. On the other hand, in peatmoss, the best result was shown at 3.0mS/cm. Therefore, the adequate concentration of nutrient solution on early growth of seedlings differed among substrates and was shown to be 3.0-5.0mS/cm in perlite, 2.0-5.0mS/cm in vermiculite, and 3.0mS/cm in peatmoss. Generally, as the concentrations of nutrient solution increased from 1.0 to 3.0mS/cm after transplanting, dry weight increased significantly in all three substrate culture. However, dry weights of tomato plants grown under high concentration of 5.0mS/cm slightly increased both at seedling stage and after transplanting.

• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.327-332
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• 1994

The effect of methylglyoxal-bis (guanylhydrazone) (MGBG) and ethylene synthesis inhibitors on adventitious root formation from soybean cotyledon in relation to ethylene production and endogenous polyamine content was investigated. Cotyledon explants cultured on rooting medium formed numerous adventitious rook on the cut surfaces after 2 weeks of culture. However when cultured on rooting medium supplemented with MGBG, the root formation was strongly inhibited, its inhibitory effect was reserved when cultured on medium with MGBG + spermine, MGBG + CoCl$_2$ and MGBG + spermine+CoC1$_2$. A slight reversion of the rooting inhibition was observed in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment, whereas it caused a significant effect in 10$^{-3}$ M MGBG +10$^{-4}$ M treatment .Ethylene production and endogenous polymine content was investgated in 10$^{-3}$ M MGBG , 10$^{-3}$ M MGBG +10$^{-5}$ Mspermine, 10$^{-3}$ M MGBG +10$^{-4}$ M CoCl$_2$and 10$^{-3}$ M MGBG +10$^{-5}$ M spermine +10$^{-4}$ M CoCl$_2$treatments. Ethylene production highest in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment was higher than control. In 10$^{-3}$ M MGBG +10$^{-5}$ M spermine + 10$^{-4}$ M CoCl$_2$ treatment, ethilene production was lowest, whereas polyamine level was highest.

• BMB Reports
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• v.32 no.5
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• pp.492-496
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• 1999

We have investigated the biochemical properties of the herpes simplex virus type 1 (HSV-1) DNA polymerase without the UL42 protein (Pol), purified from insect cells infected with a recombinant baculovirus containing the UL30 gene. BSA and DTT have inhibitory effects on dAMP incorporation. Pol showed a greater turnover rate of steady-state single nucleotide incorporation at 12 mM $MgCl_2$ than at 2 mM $MgCl_2$. However, it showed a greater processivity of DNA synthesis at lower $MgCl_2$ concentration (1 mM, 2 mM) than at a higher $MgCl_2$ concentration (12.5 mM). These results are consistent with a slow DNA dissociation at lower $MgCl_2$ concentrations. Pol does not incorporate a correct nucleotide into the primer with an incorrect nucleotide at the end; instead, it preferentially excises the incorrect nucleotide at the 3' end of the primer. Pol has DNA polymerase activity at pHs 6.5 and 7.5 but little at pHs 5.5, 8.5, and 9.5. It has exonuclease activity at pHs 6.5, 7.5, and 8.5 but little at pHs 4.5, 5.5, and 9.5. The finding that Pol has exonuclease activity but not DNA polymerase at pH 8.5 suggests that DNA binds to Pol, but deoxynucleotide binding or incorporation does not occur at pH 8.5.