Abstract
Activities of the $F_1-ATPase$ purified from Lentinus edodes were stimulated by $Fe^{3+},\;Fe^{2+},\;Cd^{2+},\;Mg^{2+},\;K^{+}\;and\;Co^{2+}$ but were inhibited by $Zn^{2+},\;Ca^{2+},\;Cu^{2+}\;and\;Ni{2+}$ ion. The enzyme activities were increased 130, 65, 65, 68, 105% and 23% by the 5mM $Fe^{3+}$, 10 mM$Fe^{2+}$, 1mM $Cd^{2+}$, 5mM $Mg^{2+}$, 5mM $K^{+}$ and 5mM$Co^{2+}$ ion addition, respectively, as compared with those not added. The enzyme activities were decreased 18, 19, 27 and 30% by 10 mM $Zn^{2+}$, 10mM $Ca^{2+}$, 0.5 mM $Cu^{2+}$ and 10 mM $Ni^{2+}$ ion, respectively. Anion effects of 10 mM ${Co_3}^{2-}$, 20 mM,$CN^{-}$ 20 mM$CH_3COO^{-}$ and 20 mM ${NO_3}^{-}$ ion were inhibited to the enzyme activities of 98, 95, 70 and 50%, respectively. As increasing of ${SO_4}^{2-}$ ion concentration, the enzyme activity was stimulated and 20 mM ${SO_4}^{2-}$ ion was shown increased of 21%.
1. 표고버섯 중 정제된 ATPase는 $Fe^{3+},\;Fe^{2+},\;Cd^{2+},\;Mg^{2+},\;K^{+}$ 및 $Co^{2+}$ 이온에 의하여 활성화 되었으나 $Zn^{2+},\;Ca^{2+},\;Cu^{2+}$ 및 $Ni^{2+}$ 이온에 의하여는 그 활성도가 억제되었다. 2. 5 mM $Fe^{3+}$, 10 mM $Fe^{2+}$, 1 mM $Cd^{2+}$, 5 mM $Mg^{2+}$, 5 mM $K^{+}$ 및 5 mM $Co^{2+}$이온은 이효소의 활성을 각각 130, 65, 65, 68, 105 및 23% 증가시켰다. 3.10 mM $Zn^{2+}$, 10 mM $Ca^{2+}$, 0.5 mM $Cu^{2+}$ 그리고 10 mM $Ni^{2+}$ 이온은 이효소의 활성을 각각 18, 19, 27% 그리고 30%의 억제효과를 보였다. 4. 10 mM ${Co_3}^{2-}$, 20 mM $CN^{-}$, 20 mM ${CH_3COO}^{-}$ 그리고 20 mM ${NO_3}^{-}$의 음이온은 이효소의 활성을각각 98, 95, 70% 그리고 50%를 억제시켰으나,${SO_4}^{2-}$ 이온은 농도증가에 따라 활성화 되었고, 20 mM 용액에서 21%로 활성도 증가의 최대치를 보였다.