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Electrochemical Behavior of Bis(2,2'-Bipyridyl) Copper(II)-Sodium Dodecyl Sulfate Solution in the Presence of $MgCl_2$ (염화마그네슘이 있을 때 비스(2,2'-비피리딜)구리(II)-도데실황산나트륨 용액의 전기화학적 거동)

  • Ko, Young Chun;Chung, Keun Ho
    • Analytical Science and Technology
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    • v.10 no.2
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    • pp.126-130
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    • 1997
  • Electrochemical behavior of 1.0mM bis(2,2'-bipyridyl)copper( II )(${Cu(bpy)_2}^{2+}$) in 100mM NaCl+27mM $MgCl_2$ solution with and without sodium dodecyl sulfate(SDS) is investigated. In the presence of SDS, $E_{pa}$ and $E_{1/2}$ of ${Cu(bpy)_2}^{2+/1+}$ shifts to the positive direction compared to the SDS free. Teh intersection of two lines on ${\Delta}E_p$ vs. -log[SDS] plot for 1.0mM $Cu(bpy)_2(NO_3)_2$ in 100mM NaCl+27mM $MgCl_2$ solution is a critical micelle concentration(CMC). Reduced form ${Cu(bpy)_2}^{1+}$ in the presence of $MgCl_2$ is more easily associated to dodecyl sulfate anion($DS^-$) than oxidized ${Cu(bpy)_2}^{2+}$. As the ionic strength is increased by the addition of multiply charged cations($MgCl_2$), the double layer becomes more compact and micelle formation is more difficult.

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The Limnological Survey and Phosphorus Loading of Lake Hoengsung (횡성호의 육수학적 조사와 인부하)

  • Kwon, Sang-Yong;Kim, Bom-Chul;Heo, Woo-Myung
    • Korean Journal of Ecology and Environment
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    • v.37 no.4 s.109
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    • pp.411-422
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    • 2004
  • A limnological survey was conducted in a reservoir, Lake Hoengsung located in Kangwondo, Korea, from July 2000 to September 2001 on the monthly basis. Phosphorus loading from the watershed was estimated by measuring total phosphorus concentration in the main tributary. Secchi disc transparency, epilimnetic (0-5 m) turbidity, chlorophyll a (Chl-a), total phosphorus (TP), total nitrogen(TN) and silica concentration were in the range of 0.9-3.5 m, 0.1-8.5 NTU, 0.3-32.4 mgChl $m^{-3}$, 5-46 mgP $m^{-3}$, 0.83-3.55 mgN $L^{-1}$ and 0.5-9.6 mgSi $L^{-1}$, respectively. Green algae and cyanobacteria dominated phytoplankton community in warm seasons, from July through October, 2000. In July a green alga (Scenedesmus sp.) was dominant with a maximum cell density of 10,480 cells mL. Cyanobacteria (Microcystics sp.) dominated in August and September with cell density of 3,492 and 295 cells mL ,respectively. Species diversity of phytoplankton was highest (2.22) in July. The trophic state of the reservoir can be classified as eutrophic on the basis of TP, Chl-a, and Secchi disc transparency. Because TP concentration was high in flood period, most of phosphorus loading was concentrated in rainy season. TP loading was calculated by multiplying TP and flow rate. The dam managing company measured inflow rate of the reservoir daily, while TP was measured by weekly surveys. TP of unmeasured days was estimated from the empirical relationship of TP and the flow rate of the main tributary; $TP=5.59Q^{0.45}\;(R^2=0.47)$. Annual TP loading was calculated to be 4.45 tP $yr^{-1}$, and the areal P loading was 0.77 gP $m^{-2}\;yr^{-1}$ which is similar to the critical P loading for eutrophication by Vollenweider's phosphorus model, 0.72 gP $m^{-2}\;yr^{-1}$.

Betaine-γ-aminobutyric Acid Transporter 1 (BGT-1/mGAT2) Interacts with the PDZ Domain of Munc-18 Interacting Proteins (Mints) (Betaine-γ-aminobutyric acid transporter 1 (BGT-1/mGAT2)과 Munc-18-interacting (Mint) 단백질의 PDZ 결합)

  • Kim, Sang-Jin;Jeong, Young-Joo;Choi, Sun-Hee;Choi, Chun-Yeon;Jun, Hee-Jae;Moon, Il-Soo;Seog, Dae-Hyun;Jang, Won-Hee
    • Journal of Life Science
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    • v.22 no.9
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    • pp.1159-1165
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    • 2012
  • The action of neuronally released ${\gamma}$-aminobutyric acid (GABA) is terminated by uptake into the neurons by GABA transporters (GATs). The mechanism underlying the stabilization and regulation of GAT2 has not yet been elucidated. We used the yeast two-hybrid system to identify proteins that interact with and, thereby, regulate betaine-${\gamma}$-aminobutyric acid transporter 1 (BGT-1/mGAT2). We found an interaction between BGT-1/mGAT2 and Munc-18-interacting proteins (Mints). The "T-H-L" motif at the C-terminal end of BGT-1/mGAT2 was essential for the interaction with Mint2 in the yeast two-hybrid assay. Mint2 bound to the tail region of BGT-1/mGAT2, but not to other GAT members. When co-expressed in HEK-293T cells, Mint2 was co-immunoprecipitated with BGT-1/mGAT2. In addition, we demonstrated the cellular co-localization of BGT-1/mGAT2 and Mint2 in the cells. These results suggest that Mint2 contributes to the regulation of BGT-1/mGAT2.

Properties of Extracellular Cytosine Deaminase from Arthrobacter sp. JH-13 (Arthrobacter sp.JH-13이 생산하는 세포외 Cytosine Deaminase의 성질)

  • Yeeh, Yeehn;Park, Jeong-Hae;Jun, Hong-Ki
    • Korean Journal of Microbiology
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    • v.23 no.3
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    • pp.177-183
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    • 1985
  • Some properties of an extracellular cytosine deaminase produced from Arthrobacter sp.JH-13 were examined after 20-80% of ammonium sulfate fractionation. Among some substrates, this enzyme utilized cytosine and 5-fluorocytosine as a substrate. The optimum pH and temperature for the activity of this enzyme were found to be near 8.0 and $40^{\circ}C$, respectively. The ensyme was more stable in 0.2M of Tris-HCl buffer than 0.2M of potassium phosphate buffer. The enzyme was generally stable below $50^{\circ}C$, but inactivated completely at $70^{\circ}C$. 1mM of $Fe^{3+},\;K^+\;and\;Na^+$ increased the enzyme activity, but 0.01mM of $Co^{2+},\;Cu^{2+},\;Ni^{2+},\;Hg^{2+},\;Ag^{2+},\;Zn^{2+},\;Ba^{2+},\;and\;Mg^{2+}$ markedly inactivated the enzyme activity. 0.1mM of p-chloromercuribenzoate, trichloroacetic acid, and N-ethylmaleimide compleyely inhibited the enzyme activity, but 0.1mM of 2-mercaptoethanol slightly increased the enzyme activity.

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Biochemical Characterization of 1-Aminocyclopropane-1-Carboxylate Oxidase in Mung Bean Hypocotyls

  • Jin, Eon-Seon;Lee, Jae-Hyeok;Kim, Woo-Taek
    • BMB Reports
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    • v.31 no.1
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    • pp.70-76
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    • 1998
  • The final step in ethylene biosynthesis is catalyzed by the enzyme 1-aminocyclopropane-1-carboxylate (ACC) oxidase. ACC oxidase was extracted from mung bean hypocotyls and its biochemical characteristics were determined. In vitro ACC oxidase activity required ascorbate and $Fe^{2+}$, and was enhanced by sodium bicarbonate. Maximum specific activity (approximately 20 nl ethylene $h^{-1}$ mg $protein^{-1}$) was obtained in an assay medium containing 100 mM MOPS (pH 7.5), $25\;{\mu}M$ $FeSO_4$, 6 mM sodium ascorbate, 1 mM ACC, 5 mM sodium bicarbonate and 10% glycerol. The apparent $K_m$ for ACC was $80{\pm}3\;{\mu}M$. Pretreating mung bean hypocotyls with ethylene increased in vitro ACC oxidase activity twofold. ACC oxidase activity was strongly inhibited by metal ions such as $Co^{2+}$, $Cu^{2+}$, $Zn^{2+}$, and $Mn^{2+}$, and by salicylic acid. Inactivation of ACC oxidase by salicylic acid could be overcome by increasing the $Fe^{2+}$ concentration of the assay medium. The possible mode of inhibition of ACC oxidase activity by salicylic acid is discussed.

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EEM Analysis of Open-Typed Cylindrical Shieldcase (양끝이 열린 실드케이스의 FEM해석)

  • Kim, Young-Hak
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.05a
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    • pp.767-769
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    • 2017
  • Four-shell open-typed shield case was analyzed using 3D FEM. Analysis Dimension was $2m{\times}2m{\times}2m$. Length of shield case was 0.6m and its diameter was 0.1m, 0.08., 0.06m and 0.04m. Thickness and permeablility of shield case was all 1mm and 50,000. The excited magneic fields were earth magneic fields, which were 24A/m in the holizontal direction and 36A/m in the vertical direction, respectively. During FEM analysis, shield case was located at the direction of holizontal magnetic field and was rotated $90^{\circ}$. Magnetic field was $4.45{\times}10^{-2}A/m$ at the direction of holizontal magnetic field and $6.66{\times}10^{-4}A/m$ at the $90^{\circ}$ rotated direction.

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Characterizations of Several Modules Relative to the Class of B(M, X)

  • Talebi, Yahya;Hosseinpour, Mehrab
    • Kyungpook Mathematical Journal
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    • v.53 no.1
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    • pp.37-47
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    • 2013
  • Let M and X be right R-modules. We introduce several modules relative to the class of B(M, X) and we investigate relation among these modules. In this note, we show if M is X-${\oplus}$-supplemented such that $M=M_1{\oplus}M_2$ implies $M_1$ and $M_2$ are relatively B-projective, then M is an X-H-supplemented module.

A STUDY OF ORAL STATUS OF MENTAL RETARDED CHILDREN (정신(精神) 박약아(薄弱兒)의 구강(口腔) 상태(狀態)에 관(關)한 고찰(考察))

  • Jhee, In-Ae
    • Journal of the korean academy of Pediatric Dentistry
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    • v.8 no.1
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    • pp.77-88
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    • 1981
  • The purpose of this study was to make a comprehensive study & evaluation of the oral status of mental retarded children. The auther examined intraorally 486 (male; 311, female;175) mental retarded children. The result was as follows; (General mental retarded children means the children who live in their parent's home, & orphan mental retarded children means the children who live in orphanage.) 1. The dft rate was 31.6% in general mental retarded children (G.m.r.c.) & 20.7% in orphan mental retarded children (O. m. r. c.). The dft index was 3.73 in G.m.r.c. & 2.15 in O.m.r.c. 2. The DMFT rate was 24.6% in female G.m.r.c., 16.7% in male G.m.r.c., 12.7% in female O.m.r.c., 8.4% in male O.m.r.c. The DMFT index was 4.94 in female G.m.r.c., 4.01 in male G.m.r.c., 1.40 in male O.m.r.c., 2.75 in female O.m.r.c. 3. The malocclusion prevalence was 57.3%. the class I malocclusion was 14.2% Class II malocclusion 19.3%, Class III malocclusion 23.5%. The children with Down's syndrome had 60.0% of class III malocclusion prevalence. 4. The dental calculus index was 1.97 in male O.m.r.c., 1.81 in female O.m.r.c., 1.30 in male G.m.r.e., 1.13 in female G.m.r.c. 5. The dental plaque index was 3.06 in female G.m.r.c., 3.00 in male Gm.r.e. 2.70 in male O.m.r,c., 2.32 in female O.m.r.c.

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A Study on the Inland Signal-fire in Chosun Dynasty (조선시대 내지봉수에 관한 연구)

  • Yoon, Jae-Woong;Lee, Chul-Young
    • Journal of architectural history
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    • v.18 no.6
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    • pp.47-64
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    • 2009
  • This research was to analyze the inland signal-fire which is the main facility of military protective duty for safety of land in chosun dynasty. The results of this research were described separately as follows. 1. Five types of site plan of signal-fire were observed, which is circle, oval, rectangular, indeterminate form. The majorities were oval type. 2. Plan configuration of brazier which is separated with circle, rectangular type roughly was constructed with circle type generally. The height of brazier was about 3~4.5m. The diameter of brazier was 1.5~2.5m. Building base that protect ground moisture and infilteration of rainfall was found in 5 inland singal-fire, the height of it was about 0.3m~2.5m. 3. The heigh to protective wall remained until present was about 1m, the depth was about average 1.2m. 4. Entrance was mainly stairs or open type and average width was 1.0~1.2m. However the depth was almost observed as 1m, originally, it was estimated it has more depth. 5. The storage of inland signal-fire has rectangular dimension, several types of $1.8{\times}1.2m{\sim}5.7{\times}4.4m$, square(間) of $1{\times}1{\sim}2{\times}1$. The building material was stone and located below or near the brazier.

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A study on the Plan Modular Establishment for the Development of Wooden Dwelling Model - Focused on the Post & Beam Structure - (목조주택 모형개발을 위한 평면모듈 설정에 대한 연구 - 기둥-보 방식 구조를 중심으로 -)

  • Kang, Man-Ho;Joo, Seok-Joong;Kim, Jae-Deok
    • Journal of the Korean housing association
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    • v.18 no.1
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    • pp.141-148
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    • 2007
  • In this research, to develope wooden house model with post & beam structure which is commensurate with domestic environment, we analyzed the size and module of a unit space and space organization of existing habitation, then, based on the result, we tried to suggest general wood house modules. For this, we investigated 98 floor plans for 30 pyeong apartment in Seoul, Gyounggi-do, Gwangju, and Jeolla-do. The results are showed as follows. 1) It has shown that 7 posts distances are available in 2 bay floor plan type - 3.0 m, 3.3 m, 3.6 m, 3.9 m, 4.2 m, 4.5 m, and 4.8 m. 2) It has shown that 8 posts distances are available in 3 bay floor plan type - 3.0 m, 3.3 m, 3.6 m, 3.9 m, 4.2 m, 4.5 m, 4.8 m, and 5.1 m. It was concluded as follows, applying the standard of regulations of horizontal modular coordination design, from $\ulcorner$the standard of modular coordination design in architecture(KSF 1525)$\lrcorner$. 1) The available widths are 3.0 m, 3.3 m, 3.6 m, 4.2 m, and 4.8 m, and the available depths are 3.0 m, 3.3 m, 3.6 m, and 4.2 m. 2) To guarantee a space through post module of a room combination, we can use a module of 3.6 m, 4.2 m, 4.8 m except 3.0 m, 3.3 m among available modules. The module investigated in post & beam structure wooden house is applied in basic 6 floor plans as follows. 1) When organize the room on the basis of tile module of 3.6 m, 4.2 m, it was possible to organize the floor plan. 2) After arranging main room, making practical application of variableness which is advantage of post & beam structure, putting to practical use of extra space that becomes the combination of atypical room such as kitchen, dining room, and bathroom, it was possible to organize the floor plan of the residence. 3) It is possible to organize whole rooms through the plan module from 3.6 m to 4.2 m, that decides the floor plan of a wooden house.