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http://dx.doi.org/10.5352/JLS.2012.22.9.1159

Betaine-γ-aminobutyric Acid Transporter 1 (BGT-1/mGAT2) Interacts with the PDZ Domain of Munc-18 Interacting Proteins (Mints)  

Kim, Sang-Jin (Department of Neurology, College of Medicine, Inje University)
Jeong, Young-Joo (Department of Biochemistry, College of Medicine, Inje University)
Choi, Sun-Hee (Department of Biochemistry, College of Medicine, Inje University)
Choi, Chun-Yeon (Department of Biochemistry, College of Medicine, Inje University)
Jun, Hee-Jae (Departments of Thoracic and Cardiovascular Surgery, College of Medicine, Inje University)
Moon, Il-Soo (Departments of Anatomy, College of Medicine, Dongguk University)
Seog, Dae-Hyun (Department of Biochemistry, College of Medicine, Inje University)
Jang, Won-Hee (Department of Biochemistry, College of Medicine, Inje University)
Publication Information
Journal of Life Science / v.22, no.9, 2012 , pp. 1159-1165 More about this Journal
Abstract
The action of neuronally released ${\gamma}$-aminobutyric acid (GABA) is terminated by uptake into the neurons by GABA transporters (GATs). The mechanism underlying the stabilization and regulation of GAT2 has not yet been elucidated. We used the yeast two-hybrid system to identify proteins that interact with and, thereby, regulate betaine-${\gamma}$-aminobutyric acid transporter 1 (BGT-1/mGAT2). We found an interaction between BGT-1/mGAT2 and Munc-18-interacting proteins (Mints). The "T-H-L" motif at the C-terminal end of BGT-1/mGAT2 was essential for the interaction with Mint2 in the yeast two-hybrid assay. Mint2 bound to the tail region of BGT-1/mGAT2, but not to other GAT members. When co-expressed in HEK-293T cells, Mint2 was co-immunoprecipitated with BGT-1/mGAT2. In addition, we demonstrated the cellular co-localization of BGT-1/mGAT2 and Mint2 in the cells. These results suggest that Mint2 contributes to the regulation of BGT-1/mGAT2.
Keywords
Neurotransmitter transporter; ${\gamma}$-aminobutyric acid transporter; betaine-${\gamma}$-aminobutyric acid transporter 1 (BGT-1); Munc-18-interacting protein 2 (Mint2); PDZ Domain;
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