• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.234-238
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• 2012

Recombinant Escherichia coli displaying organophosphorus hydrolase (OPH) was used to overcome the diffusion barrier limitation of organophosphorus pesticides. A new anchor system derived from the N-terminal domain of ice-nucleation protein from Pseudomonas syringae InaV (InaV-N) was used to display OPH onto the surface. The designed sequence was cloned in the vector pET-28a(+) and then was expressed in E. coli. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by InaV-N on the outer membrane, and the ability of recombinant E. coli to utilize diazinon as the sole source of energy, without growth inhibition, indicated its significant activity. The location of OPH was detected by comparing the activity of the outer membrane fraction with the inner membrane and cytoplasm fractions. Studies revealed that recombinant E. coli can degrade 50% of 2 mM chlorpyrifos in 2 min. It can be concluded that InaV-N can be used efficiently to display foreign functional protein, and these results highlight the high potential of an engineered bacterium to be used in bioremediation of pesticide-contaminated sources in the environment.

• 한국환경생태학회지
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• 제17권1호
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• pp.18-25
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• 2003

PCB가 산개구리 여포난자의 성숙과 프로제스테론 생성에 미치는 영향을 알아보기 위해 배양액에 일정 농도의 PCB(Arochlor 1248)를 농도별로 첨가한 후 난자들을 20시간 배양하였다. 난자의 성숙과 프로제스테론 생성을 유도하기 위하여 FPH(Frog pituitary homogenate: 0.01p.e/$m\ell$)를 사용하였으며 여포난자의 성숙율은 난자 내의 핵막 붕괴율로부터 구하였고 프로제스테론 생성은 배양액내로 분비되는 양을 조사하였다. 실험 결과 PCB는 10ppb의 농도부터 여포난자의 성숙과 프로제스테론 생성을 현저히 억제하였으며 PCB 작용의 가역성을 조사하기 위해 3시간 동안 여포난자들을 PCB에 노출시킨 후 보통 배양액으로 옮겨 계속 배양을 해 본 결과 PCB 2.5ppb에서는 가역성을 나타내었으나 5 ppb에서는 비가역적인 손상을 주었다. 이와 같이 PCB는 낮은 농도에서 난자의 성숙과 프로제스테론의 분비 등을 억제하였으며, 개구리 난자 배양계는 환경오염물질의 독성 검정에 요긴하게 사용할 수 있음을 시사하였다

• 한국광학회지
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• 제25권6호
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• pp.334-339
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• 2014

이 논문에서는 소형센트릭 카메라의 광학적 특성이 분석적, 실험적으로 연구되었다. 광학적 특성으로 소형센트릭광학계의 재귀반사 광도계수가 조사되었다. 우선, 시뮬레이션을 통해서, 이론적 재귀반사광도계수 값을 측정하였다. 두번째로, 소형센트릭 카메라 장치를 디자인 하였으며, 이후 실험을 통해 실제 장치의 재귀반사광고계수를 측정하였다. 시뮬레이션과 시험을 통해, 재귀반사광도계수의 측정치 및 실험치 모두가 (P384200CPH: $0,0042m^2/sr{\sim}0,018m^2/sr$, P285200CPH: $0,0045m^2/sr{\sim}0,0297m^2/sr$, P321450S: $0,0021m^2/sr{\sim}0,00963m^2/sr$) 1.4 ~ 4.9의 분각에서 $0,002{\sim}0,03m^2/sr$의 범위에 있음을 발견하였다. 이 결과는, 실제 장비를 구상하는데 사용된 메커니즘과 실험방법의 중요성(유효성)을 증명한다. 이에따라, 이 유효한 결과를 통해서, 이 연구는 광학공학의 이론과 실제의 발전에 기여하였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.569-572
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• 2002

Terra MODIS is one of the few space-borne sensors currently capable of acquiring radiometric data over the range of view angles. Institute of Industrial Science, University of Tokyo, has been receiving Terra MODIS data at Tokyo since May 2001 and Asian Institute of Technology at Bangkok since May 2001. They can cover whole East Asia and is expected to monitor environmental changes regularly such as deforestation, forest fires, floods and typhoon. Over eight hundred scenes have been archived in the storage system and they occupy 2 TB of disk space so far. In this study, MODIS data processing system on WWW is developed including following functions: spectral subset (250m, 500m, 1000m channels), radiometric correction to radiance, spatial subset of geocoded data as a rectangular area with latitude-longitude grid system in HDF format, generation of a quick look file in JPEG format. Users will be notified just after all the process have finished via e-mail. Using this system enables us to process MODIS data on WWW with a few input parameters and download the processed data by FTP access. An easy to use interface is expected to promote the use of MODIS data. This system is available via the Internet on the following URL from September 1 2002, "http : //webmodis.iis.u-tokyo.ac.jp/".

• Preventive Nutrition and Food Science
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• 제10권1호
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• pp.11-16
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• 2005

Enzymatic hydrolysis of dark muscle of skipjack was optimized by using response surface methodology. Three factors of independent values were pH (4.2 to 9.8), time (0.6 to 3.4 hrs) and temperature (34℃ to 76℃), and independent values were optical density and brix. The optimum conditions for enzymatic hydrolysis were pH 7.0 to 8.0, 55℃ and 3 hrs. The headspace volatile compounds of reaction flavors using the enzymatic hydrolysate, cysteine and xylose were identified by using the combination of a canister system, gas chromatography and mass selective detector. Among 67 compounds, we identified 8 sulfur-containing compounds and 7 furans which were thought to be highly related to meat-like flavors.

• Journal of Veterinary Science
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• 제22권5호
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• pp.75.1-75.10
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• 2021

Background: Programmed cell death protein-1 (PD-1) and programmed cell death ligand-1 (PD-L1) have important roles in tumor evasion of the immune system. Objectives: This study aimed to assess the diagnostic utility of circulating PD-1 and PD-L1 levels in healthy dogs and dogs with tumors. Methods: Circulating PD-1 and PD-L1 levels in the serum of 71 dogs with tumors were compared with those of 52 healthy dogs by performing enzyme-linked immunosorbent assay (ELISA). Results: The ELISA results revealed higher circulating PD-1 and PD-L1 levels in dogs with tumors (2.9 [2.2-3.7] ng/mL; median [IQR] and 2.4 [1.4-4.4] ng/mL, respectively) than in healthy dogs (2.4 [1.9-3.0] ng/mL; p = 0.012 and 1.4 [0.9-2.1] ng/mL; p < 0.001, respectively). Especially, there was a significant difference in circulating PD-1 levels between healthy dogs and dogs with malignant epithelial tumors (2.4 [1.9-3.0] ng/mL and 3.1 [2.6-4.4] ng/mL, respectively; p < 0.01). In addition, there was a significant difference in circulating PD-L1 levels between healthy dogs and dogs with lymphomas (1.4 [0.9-2.1] ng/mL and 2.7 [1.6-5.8] ng/mL, respectively; p < 0.001). Conclusion: This study indicates that circulating PD-1 and PD-L1 have potential as tumor diagnostic biomarkers in dogs with tumors.

• 한국축산시설환경학회지
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• 제19권2호
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• pp.81-88
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• 2013

We planned to study how the chemical disposal designed by SOP can affect on physicochemical compositions of the livestock excrement. According to Livestock Manure Management Scheme, we experimented in two steps; the first step, NaOH treatment-Citric acid neutralization, and then the second procedure, Thermophilic Aerobic Oxidation (TAO) system. Physicochemical compositions of the 3-days-old samples after NaOH treatment were pH 10.31, EC 24.54 mS/cm, SCOD 3,022 mg/L, T-N 4,315 mg/L, $NH{_4}^+-N$ 1,960 mg/L, and not detected E. coli.. And those of one-day-old samples after citric acid neutralization were pH 7.36, EC 32.89 mS/cm, $SCOD_{Mn}$ 12,733 mg/L, T-N 4,787 mg/L, $NH{_4}^+-N$ 2,450 mg/L, and E. coli. not detected. In contrast, the physicochemical compositions of the treatment plots after the second treatment with TAO system (72hr) were pH 9.42 EC 24.21 mS/cm, $SCOD_{Mn}$ 3,660 mg/L, T-N 3,616 mg/L, $NH{_4}^+-N$ 1,190 mg/L, and no detection of E. coli.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1550-1554
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• 2003

One hundred of lactic cultures were evaluated for their ability to inhibit hazardous microbes, such as Salmonella enteritidis, Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Bacillus cereus by agar well diffusion method. None of them showed inhibitory halo against S. enteritidis, while 27 strains showed inhibitory activity against S. typhimurium, 6 against E. coli, 9 against ampicillin resistant E. coli, 31 against L. monocytogens, 10 against B. cereus. pH of the culture does not explain for the inhibitory activity except against B. cereus. A neutralized culture from corn silage showed highest inhibitory activity against S. typhimurium, and the size of inhibitory halo was same as 10 ug/mL of ampicillin. The culture was identified to be Lactobacillus buchneri on the basis of biochemical characteristics and utilization of substrates. Using the culture as probiotics could be expected to reduce antibiotics for animal feeding.

• Ocean and Polar Research
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• 제22권1호
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• pp.25-36
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• 2000

The polychaeta fauna of the benthos and fouling of the northwestern part of the East Sea was studied during the period of 1971-1998. Three introduced species of polychaetes: Hydorides elegans (Haswell), Polydora limicola Annenkova, and Pseudopotamilla occelata Moore were found. H. elegans was discovered only on the artificial surfaces in Golden Horn Inlet (port Vladivostok), where this species may occur because of hermal pollution due to the discharge of warm waters of the water cooling system of Thermal-Electric Power Station-2 (TEPS-2) in Vladivostok which has been in function since 1971. The abundant population of H. elegans exists in the bay throughout the year and is capable of reproduction. The biomass of H. elegans may reach several $kg/m^2$ in August-September. P. limicola was found at the same time in the fouling of hydrotechnical structures of Vladivostok, Nakhodka, Holmsk and Uglegorsk ports with a biomass of $1-3kg/m^2$. Slow introduction of P. limicola occurs by coastal sail ships at present. The invasion of P. occelata into Peter the Great Bay may be an example of introduction and subsequent naturalization, which produced considerable changes in the structure of benthic communities. The three species of polychaetous sessile organisms and their invasion occurred by ocean and coasters sea-going ships (unintentional transport vectors). H. elegans and P. occelata were most probably transported to the northwestern part of the East Sea from Japan, and P.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2475-2478
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• 2016

Background: Laparoscopic partial nephrectomy is one of the major surgical techniques for small renal masses. However, it is difficult to manage cutting and suturing procedures within acceptable time periods. To overcome this difficulty, we applied a three-dimensional (3D) video system with laparoscopic partial nephrectomy, and evaluated its utility. Materials and Methods: We retrospectively enrolled 31 patients who underwent laparoscopic partial nephrectomy between November 2009 and June 2014. A conventional two-dimensional (2D) video system was used in 20 patients, and a 3D video system in 11. Patient characteristics and video system type (2D or 3D) were recorded, and correlations with perioperative outcomes were analyzed. Results: Mean age of the patients was $55.8{\pm}12.4$, mean body mass index was $25.7{\pm}3.9kg/m^2$, mean tumor size was $2.0{\pm}0.8cm$, mean R.E.N.A.L nephrometry score was $6.9{\pm}1.9$, and clinical stage was T1a in all patients. There were no significant differences in operative time (p=0.348), pneumoperitoneum time (p=0.322), cutting time (p=0.493), estimated blood loss (p=0.335), and Clavien grade of >II complication rate (p=0.719) between the two groups. However, warm ischemic time was significantly shorter in the 3D group than the 2D group (16.1 min vs. 21.2min, p=0.021), which resulted from short suturing time (9.1 min vs. 15.2 min, p=0.008). No open conversion occurred in either group. Conclusions: A 3D video system allows the shortening of warm ischemic time in laparoscopic partial nephrectomy and thus may be useful in improving the procedure.