Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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Effect of PCB on the Oocyte Maturation and Proges- terone Production of Frog, Rana dybowskii in Vitro

  • 고선근 (호남대학교 자연과학대학 자연과학부) ;
  • 이두표 (호남대학교 자연과학대학 자연과학부)
  • Published : 2003.04.01
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Abstract

In order to know the effects of PCB(Arochlor 1248) on the oocyte maturation and proges-terone(P$_4$) production by FPH(Frog pituitary homogenate: 0.01 p.e/$m\ell$) of frog in vitro, the oocytes were cultured for 20 hours in presence of the PCB at various concentrations and exam-ined their maturation(germinal vesicle breakdown: GVBD) rates and P$_4$ levels secreted by the oocyte in the culture medium. The results show that PCB concentration of 10 ppb suppressed the maturation of the oocytes and secretion of P$_4$. To examine the reversibility of the inhibitory effects, the oocytes were exposed to the PCB only for 3 hours, and then transferred to plain medium and cultured further for 17 hours. The oocytes were recovered from the toxic effect of the PCB when they were exposed to 2.5 ppb, but not to 5 ppb of the PCB. These results indi-cate that PCB suppress the maturation of oocytes and secretion of p, at low concentration, sug-gesting that the frog oocyte culture system can be used as a useful tool to evaluate the toxicity of the pollutants in the environment.

PCB가 산개구리 여포난자의 성숙과 프로제스테론 생성에 미치는 영향을 알아보기 위해 배양액에 일정 농도의 PCB(Arochlor 1248)를 농도별로 첨가한 후 난자들을 20시간 배양하였다. 난자의 성숙과 프로제스테론 생성을 유도하기 위하여 FPH(Frog pituitary homogenate: 0.01p.e/$m\ell$)를 사용하였으며 여포난자의 성숙율은 난자 내의 핵막 붕괴율로부터 구하였고 프로제스테론 생성은 배양액내로 분비되는 양을 조사하였다. 실험 결과 PCB는 10ppb의 농도부터 여포난자의 성숙과 프로제스테론 생성을 현저히 억제하였으며 PCB 작용의 가역성을 조사하기 위해 3시간 동안 여포난자들을 PCB에 노출시킨 후 보통 배양액으로 옮겨 계속 배양을 해 본 결과 PCB 2.5ppb에서는 가역성을 나타내었으나 5 ppb에서는 비가역적인 손상을 주었다. 이와 같이 PCB는 낮은 농도에서 난자의 성숙과 프로제스테론의 분비 등을 억제하였으며, 개구리 난자 배양계는 환경오염물질의 독성 검정에 요긴하게 사용할 수 있음을 시사하였다

Keywords

References

  1. Atlas, E., and C.S. Giam(1981) Global transport of organic pollutants: Ambient concentrationsin the remote marine atmosphere. Science 211: 163-165 https://doi.org/10.1126/science.211.4478.163
  2. Blaustein, A.R.(1994) Chicken Little or Nero's fiddle? A perspective on declining amphibian populations. Herpetologica 50(1): 85-97
  3. Blechly, J.D.(1984) Polychlorinated biphenyls: Productions, current use and possible rate of future disposal in OECD member countries. In Proceedings of PCB Seminar, Ministry of Housing, Physical Planning and Environment. Netherlands. pp. 343-372
  4. Bray T, RE. Sicard(1982) Correlation among the changes in the levels of thyroid hormones, thyrotopin and thyrotropin releasing hormone during the developing of Xenopus laevis. Exp. Cell Biol. 50: 101
  5. Delong, R.L., W.G. Gilmartin and J.G. Simpson(1973) Premature births in California sealions: Association with high organochlorine pollutant residue levels. Science, 181: 1168-1170 https://doi.org/10.1126/science.181.4105.1168
  6. Fischer, W.J. and D.R Dietrich(2000) Toxicity of the cyanobacterial cyclic toxins microcystin -LR and RR in early life-stages of the African clawed frog(Xenopus laevis). Aquatic Toxicology. 49: 189-198 https://doi.org/10.1016/S0166-445X(99)00079-X
  7. Fritz, A., D.L. Gorlick, and G.D. Burd(1996) Neurogenesis in the olfactory bulb of the frog Xenopus laevis shows unique patterns during embryonic development and metamorphosis. Int. J. Deel. Neuroscience. Vol. 14: 931-943 https://doi.org/10.1016/S0736-5748(96)00054-8
  8. Galton V.A.(1992) The role of thyroid hormone in amphbian metamorphosis. TEM 3: 96
  9. Guruge, K.S., H. Tanaka and S, Tanabe(200l) Concentration and toxic potential of polychlorinated biphenyl congeners in migratory oceanic birds from the North Pacific and Southern Ocean. Marine Environmental Research, in press.
  10. Gutleb, A.C, J. Appelman, M.C. Bronkhorst J.H.J. van den Berg, A. Spenkelink, A. Brouwer, A.J. Murk(1999) Delayed effect of pre- and early-life time exposure to polychlorinated biphenyls on tadpoles of two amphibian species(Xenopus laevis and Rana temporaria). Environmental Toxicology and Pham-acology 8: 1-14 https://doi.org/10.1016/S1382-6689(99)00023-X
  11. Helender, B., M. Olsson, and L. Reutergardh(1982) Residue levels of organochlorine and mercury compounds in unhatched eggs and the relationships to breeding success in white tailed sea eagles Haliaeetus albicilla in Sweden. Holarctic Ecol. 5: 346-366
  12. Helle, E., M. Olsson, and S. Jensen(1976) DDT and PCB levels and reproduction in ringed seal from the Bothnian Bay. Ambio, 5: 188-189
  13. Jensen, A.A.(1987)Polychlorobiphenyls(PCBs), polychlrodibenzo-p-dioxines(PCDDs) and polychlrorodibenzo-furans(PCDFs) in human milk, blood, and adipose tissue. Sci. Total. Environ, 64: 259-293 https://doi.org/10.1016/0048-9697(87)90250-6
  14. Kwon, H. B., C. H. Choi, and C. G.Choi(1988) Studies on the oocyte maturation of korean frogs(R, dybowskii and R. nigromaculata) in vitro. Korean J. Zool. 31: 87-94
  15. Kwon, H.B., J.Y. Kim and S.K. Ko(1990) Progesterone production and oocyte maturation of frog(Rana nigromaculata and Rana rugosa) follicle in vitro. Korean J. Zool. 33: 175-182
  16. Ko, S.K and D.P. Lee(1997) Effect of heavy metal ions on the oocyte maturation of frog, Rana dybowskii in vitro. Kor. J. Env. Eco. 11(3): 310-315
  17. Lin, Y. and A.W. Schuetz(1985) Intrafollicular action of estrogen in regulating pituituaryinduced ovarian progesterone synthesis and oocyte maturation in Rana pipiens: Temporal relationship and locus of action. Gen. Compo Endocrinol. 58: 421-435 https://doi.org/10.1016/0016-6480(85)90115-7
  18. Masui, Y. and H.J. Clarke(1979) Oocyte maturation. Int. Rev. Cytol. 57: 185-281 https://doi.org/10.1016/S0074-7696(08)61464-3
  19. Mondou PM, JC. Kaltenbach.(1979) Thyroid concentrations in blood serum and pericardial fluid of metamorphosing tadpoles and adult frogs. Gen. Compo Endocrinol. 39: 343 https://doi.org/10.1016/0016-6480(79)90131-X
  20. Pechman, J.H.K, and H.M. Wilbur(1994) Putting declining amphibian populations into perspective: Natural fluctuations and human impacts. Herpetologica 50: 65-84
  21. Peakall, D.B., T.J. Cade, e.M. White, and J.R. Hauge(1975) Organochlorine residues in Alaskan peregrines. Pesticide Monitoring Journal, 8: 255-260. 25
  22. Phillips, D.J.H.(1980) Quantitative aquatic biological indicators: Their use to monitor trace element and organochlorine pollution. Applied Sci. Publ, London.
  23. Reijinders, P.J.H.(1986) Reproductive failure in common seals feeding on fish from polluted costal waters. Nature, 324: 456-457 https://doi.org/10.1038/324456a0
  24. Schmidt, H., and G. Schultz(1881) Uber benzidin(a-di-amidodiphenyl). Ann. Chem. Liebigs. 207: 320 https://doi.org/10.1002/jlac.18812070306
  25. Schuetz A. W.(1967) Effect of steroids on the germinal vesicle of oocytes of the frog(Rana pipiens) in vitro. proc. soc. Exp. Biol, Med.124: 1307-1310 https://doi.org/10.3181/00379727-124-31993
  26. Schuetz A. W.(1985) Local control mechanisms during oogenesis and folliculogenesis In: develpomental Biology, Vol. 1. Oogenesis.(Browder, L. W. ed.) Plenum press, New York and London, pp. 3-83
  27. Smith, L. D. and RE. Ecker(1971) The interaction of steroids with Rana pipiens oocytes in the induction of maturation. Dev. Biol. 25: 232-2117 https://doi.org/10.1016/0012-1606(71)90029-7
  28. Takeda, N.(l997) The metabolism of biogenic monoamines during embryogenesis and metamorphosis in two anuran apecies. Gen. Comp. Endocrinol. 106: 361-373 https://doi.org/10.1006/gcen.1997.6885
  29. Tanabe, S., H. Iwata, and R Tatsukawa(1994) Global contamination by persistent organoclorines and their ecotoxicologial impact on marine mammals. The Science of the Total Environment, 154: 163-177 https://doi.org/10.1016/0048-9697(94)90086-8
  30. Tanabe, S., N. Kannan, A.N. Subramanian, S. Watanabe and R. Tatsukawa(1987) Highly toxic coplanar PCBs: Occurrence, source, persistency and toxic implications to wildlife and humans. Environ Pollut., 47: 41-62 https://doi.org/10.1016/0269-7491(87)90120-5
  31. Tanabe, S., T. Mori, and R Tatsukawa(1984) Bioaccumulation of DDTs and PCBs in the southern mink whle(Balaenoptera acutorostrata). Mem. Natl. Inst. Pola Res. 32: 140-150
  32. Tanabe, S. and R. Tatsukawa(1986) Distribution, behavior and load of PCB in the oceans. In PCBs in the environment, Vol.1, ed, by J.S. Waid, pp. 143-161. Boca Raton, Florida, CRC Press.
  33. Wake, D.B.(1991) Declining amphibian populations. Science 253: 860 https://doi.org/10.1126/science.253.5022.860