• Korean journal of applied entomology
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• 제46권1호
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• pp.131-139
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• 2007

From the course of screening of useful enzyme producing microorganism from insect guts, we isolated 9 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram negative bacteria grouped on Serratia sp., Pseudomonas sp., and Burkholderia sp.. Among them, an excellent lipase producing strain, Burkholderia sp. HY-10 identified by 16S rDNA analysis and biochemical methods, was further studied its lipase producing characteristics. It was isolated from a longcorm beetle, Prionus insularis and showed cell density dependent lipase producing activity in the culture media that contained olive oil as a carbon source. Maximum lipase production was achieved in the M9 media containing 0.5% yeast extract and 0.5% olive oil when cultured at $30^{\circ}C$ for 36-42 hrs.

• Asian-Australasian Journal of Animal Sciences
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• 제22권6호
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• pp.765-773
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• 2009

Sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN) genes play an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. This study used polymorphisms in the intron 5 of bovine SREBF1 and in the thioesterase (TE) domain of FASN genes to evaluate their associations with beef fatty acid composition. A previously identified 84-bp indel (L: insertion/long type and S: deletion/short type) of the SREBF1 gene in Korean cattle had significant associations with the concentration of stearic (C18:0), linoleic (C18:2) and polyunsaturated fatty acids (PUFA). The stearic acid concentration was 6.30% lower in the SS than the LL genotype (p<0.05), but the linoleic and PUFA contents were 11.06% and 12.20% higher in SS compared to LL (p<0.05). Based on the sequence analysis, five single nucleotide polymorphisms (SNPs) g.17924G>A, g.18043C>T, g.18440G>A, g.18529G>A and g.18663C>T in the TE domain of the FASN gene were identified among the different cattle breeds studied. Among these, only g.17924 G>A and g.18663C>T SNPs were segregating in the Hanwoo population. The g.17924G>A SNP is a non-synonymous mutation (thr2264ala) and was significantly associated with the contents of palmitic (C16:0) and oleic acid (C18:1). The oleic acid concentration was 3.18% and 2.79% higher in Hanwoo with the GG genotype than the AA and AG genotypes, respectively (p<0.05), whereas the GG genotype had 3.8% and 4.01% lower palmitic acid than in those cattle with genotype AA and AG, respectively (p<0.05). Tissue expression data showed that SREBFI and FASN genes were expressed in a variety of tissues though they were expressed preferentially in different muscle tissues. In conclusion, the 84-bp indel of SREBF1 and g.17924G>A SNP of the FASN gene can be used as DNA markers to select Hanwoo breeding stock for fatty acid composition.

• Journal of Life Science
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• 제10권3호
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• pp.307-316
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• 2000

Bacillus circulans S-1 extracellular pullulan 6-glucanohydrolase (EP) (EC 3.2.1.41) has been characterized with a purified enzyme of 140 kDa. The N-terminal amino acid sequence of the purified enzyme was P-L-N-M-S-Q-P. The enzyme displayed a temperature optimum of around $60^{\circ}C$ and a pH optimum of around pH 9.0. The enzyme was stable to incubation from pH 4.0 to pH 11.0 at $4^{\circ}C$ for 48h. The presence of substrate allowed the protection of the enzyme from heat inactivation. The activity of the enzyme was stimulated by several metal ions such as Mn2+ and Ca2+. The enzyme had an apparent Km of 7.92 mg/ml for pullulan. The purfied enzyme completely hydrolysed pullulan to maltotriose.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• 제33권2호
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• pp.131-142
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• 2015

In order to support the development of a cost-effective river bathymetric system, this research has focused on modeling GPS observables, which are obtained by array of five single-frequency receivers (i.e., two references and three rovers) to estimate the high accurate kinematic position, and the surveying vessel altitude. Also, by applying all GPS measurements as multiple-baselines with constraining rover baselines, we derived the socalled ‘kinematic network model.’ From the model, the integer-constrained least-squares (LS) for position estimation and the implicit LS for attitude determination were implemented, while a series of simulation tests with respect to the baseline lengths around 2km performed to demonstrate its accuracy analysis. The on-the-fly (OTF) ambiguity resolution tests revealed that ninety-nine percents of time-to-fix-first ambiguity (TTFF) can be decided in less than two seconds, when the positioning accuracy of ambiguity-fixed solutions was assessed as the greater than or equal to one and two centimeters in horizontal and vertical, respectively. Comparing to the GPS-derived attitudes, the achievable accuracy gradually descended in sequence of yaw, pitch and roll due to the antenna geometric configuration. Furthermore, the RMSE values for the baseline lengths of three to six meters were within ±1′for yaw, and less than ±10′and ±20′for pitch and roll, respectively, but those of between six to fifteen meters were less than ±1′for yaw, ±5′for pitch, and ±10′for roll.

• The Korean Journal of Mycology
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• 제45권3호
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• pp.246-250
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• 2017

Sclerotium rot was observed on mungbean (Phaseolus radiatus L.) plants cultivated in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services in September 2015. The progression of rot was initially observed as water-soaked lesions on several parts of the affected plant. Severely infected plants were blighted and eventually died. White mycelial mats spread over the lesions and numerous sclerotia formed on stems near the soil line. The sclerotia were globoid in shape, 1~3 mm in size, and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$ and the hyphal width was $4{\sim}8{\mu}m$. Typical clamp connections were observed on the hyphae of fungus grown on PDA. For molecular identification, the complete internal transcribed spacer (ITS) ribosomal DNA (rDNA) of the causal fungus was sequenced and analyzed. Based on the mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, the fungus was identified as Sclerotium rolfsii. This is the first report of Sclerotium rot on mungbean caused by S. rolfsii in Korea.

• Journal of Life Science
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• 제21권5호
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• pp.671-677
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• 2011

We previously reported the isolation and characterization of a gene, SCA1 (for soybean $Ca^{2+}$-ATPase 1), encoding a calmodulin-regulated $Ca^{2+}$-ATPase that is located in the plasma membrane in soybean. Here, a $Ca^{2+}$-ATPase designated as SCA2 was isolated from soybean. The two $Ca^{2+}$-ATPases, SCA1 and SCA2, share a remarkably high degree of similarity (78%). Ten transmemebrane domains were predicted by hydropathy analysis. Using gel overlay assays, CaM was found to bind to SCA2 in a $Ca^{2+}$-dependent manner. Southern blot analysis revealed the presence of two copies of the $Ca^{2+}$-ATPase gene in the soybean genome. An N-terminal truncation mutant that deletes sequence through the putative calmodulin binding site was able to complement a yeast mutant (K616) that was deficient in two endogenous $Ca^{2+}$ pumps. Our results indicate that SCA2 is structurally highly conserved with type IIB $Ca^{2+}$ pumps in plants.

• Korean Journal of Microbiology
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• 제28권2호
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• pp.91-97
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• 1990

Complementary DNA (cDNA) coding for human cytoplasmic superoxide dismutase (SOD1) (superoxide: superoxide oxidoreductase E.C.1.15.1.1) was isolated from human liver cDNA library of $\lambda$gt11 by in situ plaque hybridization. The insery cDNA gas the 5' untranslational region (UTR) and 3'UTR of SOD1 gene. Polymerase Chain Reaction (PCR) method was used fro subcloning of SOD1 structural gene. Using synthetic sense strand primer (24mer) containing a start codon and antisense strand primer (24mer), SOD1 structural gene was selectively amplified. Amplified DNA was directly cloned into the HincII site of pUC19 plasmid. Insery cDNA was subcloned into M13 mp19 and sequenced by dideowy chain termination method with Sequenase. The nucleotide sequence of insert cDNA had an open reading frame (ORF) coding for 153 amino acid residues. The structural gene of cytoplasmic SOD was placed under the control of bacteriophage $\lambda P_{L}$ regulatory sequences, generating a highly efficient expression plasmid. The production of human SOD1 in E. coli cells was about 7% of total cellular proteins and recombinant human SOD1 possessed its own enzymatic acitivity.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제19권3호
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• pp.382-388
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• 2018

In this study, the SELEX method was used to screen for and select aptamers with high selectivity and affinity for Streptococcus mutans, which is the causative agent of dental caries. Aptamers are single stranded oligonucleotides of DNA or RNA with high selectivity and affinity for target molecules because of their specific three-dimensional structures that are used to collect biometric information. When compared to antibodies in vitro, aptamers are more advantageous because of their ease of use in the screening process, low cost, chemical stability, and lack of restrictions on the target molecules. We sorted DNA aptamers, which contain 44 bp or 38 bp primer sequences in 5' and 3', 39 bp random sequences in the middle.We then analyzed the character and affinity to S. mutans. Aptamers of specific oligonucleotides that combine with S. mutans were selected and these results are selectively fused to S. mutans. The results confirmed that DNA aptamers can be used for rapid diagnosis and treatment of dental caries caused by bacteria of the oral cavity, namely S. mutans.

• Journal of Korean Society for Atmospheric Environment
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• 제22권1호
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• pp.99-106
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• 2006

Aircraft measurements have been executed for the purpose of monitoring the long range transported air pollution and estimation of air pollutant in/out-flux over the Yellow sea. Total 74 missions of measurements have been done since 1997, mainly in spring and fall. The main study domain was over $124^{\circ}$E $/sim$ $124^{\circ}$E, $35^{\circ}$N $/sim$ $37^{\circ}$N below 3,000m. In long-term trends, mixing ratios of $SO_{2}$N were around 2 ppbv expect in summer ( < 1 ppbv). NOx exhibited 24 ppbv and have no clear annual trends over the Yellow Sea. The concentrations of 03 were 51, 58, 41 ppbv in spring, summer and fall-winter, respectively. Backward trajectory was performed for three days to investigate the source regions of the air mass. Six regions were divided around Korea peninsular centering at $36^{\circ}$N, $126^{\circ}$E. I, II, III, IV and V regions represents in sequence northeast China and Siberia, Sandong peninsula and Balhae gulf, Sanghi and southern China, the south Pacific included Jeju island and the East sea included Japan. L region correspond to the airmass from Korea peninsula. Influx of $SO_{2}$N was approximately five times higher than outflux in yearly flux variation and showed a decreasing long-term trend since 1998. NOx outflux was average 0.095 ton/km/hr and three times higher than $SO_{2}$ outflux. In/out flux of 03 showed even distribution in yearly basis except 2002 (influx 5.45 ton/km/hr). The transported amounts from I, II, III regions were much higher than those from other region. In seasonal flux variation, influx levels of gas phases were the lowest in summer and the levels gradually increased from fall toward spring. As a result, transport of pollutants begins from fall and prevails in winter and spring.

• Journal of the Korean Society of Radiology
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• 제12권4호
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• pp.475-480
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• 2018

In lower abdominal MRI scan, patients have been tested by physically contacting with the body array coil. In this study, we have designed the acrylic assistant equipment (ACR) which allows the contactless scan of the patient to the coil and evaluated the feasibility by comparing the acquired images with ACR to those obtained without ACR. We tested 10 cases (F: 5, m: 5) by using the Ingenia $3.0T^{TM}$ MR system and dStreamTM torso coil (Philips Healthcare, Netherlands). We implemented T1 AX TSE and eTHRIVE (GRE) techniques. The scanned images were quantitatively and qualitatively assessed. In qualitatively, the TSE shows 4.44 and 4.56 mean values with and without the ACR and 4.34 and 4.28 at the GRE, respectively. In quantitatively, the TSE shows 12.15 CNR, 17.95 SNR and 12.71 CNR, 18.96 SNR with and without the ACR. And GRE shows 17.72 CNR, 22.59 SNR and 18.26 CNR, 24.47 SNR with and without the ACR, respectively. We have designed and implemented the acrylic assistant equipment to lower abdominal patients. Our data indicate that it is possible to obtain similar image qualities to current lower abdominal MRI scan without the physical contact to the patient.