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http://dx.doi.org/10.5762/KAIS.2018.19.3.382

A novel anti-adhesion agent using DNA aptamers for Streptococcus mutans  

Park, Byung-Ju (Department of Oral Biochemistry, School of Dentistry, Chonnam National University)
Ohk, Seung-Ho (Department of Oral Microbiology, School of Dentistry, Chonnam National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.19, no.3, 2018 , pp. 382-388 More about this Journal
Abstract
In this study, the SELEX method was used to screen for and select aptamers with high selectivity and affinity for Streptococcus mutans, which is the causative agent of dental caries. Aptamers are single stranded oligonucleotides of DNA or RNA with high selectivity and affinity for target molecules because of their specific three-dimensional structures that are used to collect biometric information. When compared to antibodies in vitro, aptamers are more advantageous because of their ease of use in the screening process, low cost, chemical stability, and lack of restrictions on the target molecules. We sorted DNA aptamers, which contain 44 bp or 38 bp primer sequences in 5' and 3', 39 bp random sequences in the middle.We then analyzed the character and affinity to S. mutans. Aptamers of specific oligonucleotides that combine with S. mutans were selected and these results are selectively fused to S. mutans. The results confirmed that DNA aptamers can be used for rapid diagnosis and treatment of dental caries caused by bacteria of the oral cavity, namely S. mutans.
Keywords
anti-adhesion; aptamer; SELEX; Streptococcus; mutans;
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1 H. S. Yoon, "Dental Fear Level according to Oral Symptom Awareness in College Students, Journal of the Korea Academia-Industrial cooperation Society, vol. 17(12), pp. 198-204, 2016. DOI: https://doi.org/10.5762/KAIS.2016.17.12.198   DOI
2 J. M. Tanzer, M. L. Freedman, R. J. Fitzgerald, and R. H. Larson, "Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans", Infect. Immun., vol. 10, pp. 197-203, 1974.
3 S. Hamada, T. Ooshima, N. Masuda, J. Mizuno, and S. Sobue, "Inhibition of rat dental caries by dextranase from a strain of Spicaria violacea", Japan. J. Microbiol., vol. 20, pp. 321-330, 1976. DOI: https://doi.org/10.1111/j.1348-0421.1976.tb00994.x   DOI
4 K. Nakahara, S. Kawabata, H. Ono, K. Ogura, T. Tanaka, T. Ooshima, and S. Hamada, "Inhibitory effect of Oolong tea polyphenols on glycosyltransferases of mutans Streptococci", Appl. Environ. Microbiol., vol. 59, pp. 968-973, 1993.
5 D. M. Spinell, and R. J. Gibbons, "Influence of culture medium on the glycosyltransferase and dextran-binding capacity of Streptococcus mutans 6715 cells", Infect. Immun., vol. 10, pp. 1148-1151, 1974.
6 T. Koga, S. Sato, T. Yakushiji, and M. Inoue, "Separation of insoluble and soluble glucan-synthesizing glycosyltransferases of Streptococcus mutans OMZ176 (serotyped)", FEMS Microbiol. Lett., vol. 16, pp. 127-130, 1983. DOI: https://doi.org/10.1111/j.1574-6968.1983.tb00272.x   DOI
7 S. Hamada, and H. D. Slade, "Chemical and immunological characterization of Type e polysaccharide antigen of Streptococcus mutans", Infect. Immun., vol. 14, pp. 68-76, 1976.
8 S. Hamada, K. Gill, and H.D. Slade, "Chemical and immunological properties of the Type f polysaccharide antigen of Streptococcus mutans", Infect. Immun., vol. 14, pp. 203-211, 1976.
9 K. Fukui, T. Moriyama, Y. Miyake, K. Mizutani, and O. Tanaka, "Purification and properties of glycosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans., Infect. Immun., vol. 37, pp. 1-9, 1982.
10 K. Fukushima, R. Motoda, and T, Ikeda, "Resolution of Streptococcus mutans glycosyltransferases into two components essential to water-insoluble glucan synthesis", FEBS Letters, vol. 128, pp. 213-216, 1981. DOI: https://doi.org/10.1016/0014-5793(81)80083-X   DOI
11 S. H. Lee, M. S. Lee, "The Study of Physiological and Antimicrobial Activities on the Citrus Junos Extracts with its Textures and Skin, Journal of the Korea Academia-Industrial cooperation Society, vol. 18(4), pp. 67-74, 2017. DOI: https://doi.org/10.5762/KAIS.2017.18.4.67
12 S. Hamada, and H.D. Slade, "Biology, Immunology, and cariogenicity of Streptococcus mutans", Microbiol. Rev., vol. 44, pp. 331-384, 1980.
13 T. J. Montville, C. L. Cooney, and A.J. Sinskey, "Streptococcus mutans dextranase", Adv. Appl. Microbiol., vol. 24, pp. 55-84, 1978. DOI: https://doi.org/10.1016/S0065-2164(08)70636-1
14 S. Hamada, and M. Torii, "Interaction of glycosyltransferase from Streptococcus mutans with various glucans", J. Gen. Microbiol., vol. 116, pp. 51-59, 1980.
15 J. H. Choi, and S. H. Ohk, "Antibacterial Activity of Fraviola extract to nhibit the Staphylococcus epidermidis ", Journal of the Korea Academia-Industrial cooperation Society, vol. 18(5), pp. 667-673, 2017. DOI: https://doi.org/10.5762/KAIS.2017.18.5.667
16 J. H. Choi, and S. H. Ohk, "Evaluations on Antioxidant Effect of Water Extract from Graviola Leaves", Journal of the Korea Academia-Industrial cooperation Society, vol. 18(6), pp. 129-135, 2017. DOI: https://doi.org/10.5762/KAIS.2017.18.6.129
17 A. D. Ellington, and J. W. Szostak, "In vitro selection of RNA molecules that bind specific ligands", Nature, vol. 346, pp. 818-822, 1990. DOI: https://doi.org/10.1038/346818a0   DOI
18 C. Tuerk, and L. Gold, "Systematic evolution of ligands by exponential enrichment: R ligands to bacteriophage T4 DNA polymerase", Science, vol. 249, pp. 505-510, 1990. DOI: https://doi.org/10.1126/science.2200121   DOI
19 N. S. Que-Gewirth, and B. A. Sullenger, "Gene therapy progress and prospects: RnA aptamers", Gene Ther., vol. 14, pp. 283-91, 2007. DOI: https://doi.org/10.1038/sj.gt.3302900   DOI
20 B. J. Paster, S. K. Boches, J. L. Galvin, R. E. Ericson, C. N. Lau, V. A. Levanos, A. sahasrabudhe, and F. E. Dewhirest, "Bacterial diversity in human subfingival plaque", J. Bacteriol., vol. 183, pp. 3770-3783, 2001. DOI: https://doi.org/10.1128/JB.183.12.3770-3783.2001   DOI
21 R. Stoltenburg, C. Reinemann, and B. Strehlitz, "SELEX - A (r)evolutionary method to generate high-affinity nucleic acid ligands", Biomolecular Engineering, vol. 24(4), pp. 381-403, 2007. DOI: https://doi.org/10.1016/j.bioeng.2007.06.001   DOI
22 B. K. Kim, and B. G. Park, "Study on Optical Characteristics of 8-Hydroxyquinoline Synthesized Derivative as Sensing Material of the Fiber-Optic Copper Ion Sensor in Aqueous Environment" Journal of the Korea Academia-Industrial cooperation Society, vol. 18(12), pp. 100-105, 2017. DOI: https://doi.org/10.5762/KAIS.2017.18.12.100