Ko Y. M.;Kim D. W.;Kim K. E.;Shin S. C.;You S. J.;Ahn B. K.;Kang C. W.
Korean Journal of Poultry Science
/
v.31
no.4
/
pp.245-253
/
2004
This study was conducted to investigate the effects of dietary yeast culture containing rPST on growth performances and the characteristics of body compositions in broiler chickens. A total of 460 Ross male broiler chicks aged 2-day-old were fed one of five experimental diets; Control(devoid of all), TI (with $0.1\%$ antibiotics; chlorotetracycline), T2 (with $0.1\%$ rPST-yeast culture), T3 (with $0.2\%$ rPST-yeast culture) or T4 (with $0.2\%$ SC yeast culture) for 6 weeks. Feed consumption and body weights were measured weekly. At week 5 of the experiment, 10 chicks were randomly selected and sacrificed. The relative weights of each organ and the chemical composition of edible meat were measured. The enzyme activity, total cholesterol, Ca and P were also determined. Tibial weight, bone strength and chemical composition were investigated. There were no significant differences in feed intake and feed conversion ratios among the treatments throughout the experimental period. Body weight gains tended to be increased by feeding of diets containing rPST-yeast culture. The relative weight of breast muscle in T3 group was significantly higher than that of the control (P<0.05). The moisture contents of breast meat in groups fed diets containing rPST-yeast culture or SC yeast culture were significantly increased as compared with those of the control and n. However, the contents of crude protein and ether extract were not affected by feeding of rPST-yeast culture. There were no significant differences in GOT, total cholesterol, Ca and P. The relative weight and strength and proximal composition of the tibia were also not affected by dietary treatments. These results indicated that dietary rPST-yeast culture may be a valuable alternative for optimizing growth performances, particularly for improving the yield of breast muscle.
Yoon C.;Na C. S.;Park J. H.;Han S. K.;Nam Y. M.;Kwon J. T.
Korean Journal of Poultry Science
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v.31
no.4
/
pp.229-235
/
2004
A study was conducted to examine the effect of dietary supplementation of multiple probiotics (EM) on growth performance, blood cholesterol, intestinal micro flora, and fecal gas emission in broiler chicks. A total of 450 one day old male broiler chicks (Ross $\times$ Ross) were divided into six treatments with five replications in each treatment for five weeks. Treatments were factorially designed with two levels of diet containing probiotics (DW; 0, $0.2\%$) and three levels of drinking water containing probiotics (DW; 0, 0.01, $0.1\%$). Basal diets contained $21.5\%$ CP and 3,100 kcal/kg ME for starting and $19\%$ CP and 3,100 kcal/kg ME for finishing period. Weight gain, feed intake, and feed conversions of birds fed with probiotics were not significantly different between Ds. Total cholesterol and triglyceride levels were significantly lower (P<0.05) in birds fed with DW $0.01\%$ or $0.1\%$ compared with no probiotics group, but there was no significant difference between D treatments. The number of E. coli, Salmonella and Lactobacillus in the ileum and cecum of the birds fed multiple probiotics were not significantly different from those of no probiotic groups. There were no significant differences in the $CO_2$ gas emissions of fecal between birds fed with Ds or among birds fed with DW. However, $NH_3$ gas emissions of DW $0.1\%$ were significantly lower (P<0.05) than DW $0\%$. In the results of this study, supplementation of probiotics tended to decrease the serum cholesterol and triglyceride compared to those of control groups and reduction of fecal $NH_3$ gas emission.
This study was performed far development of new analytical method of beet red in foods. In this study, analysis of beet red in foods has been carried out by detection of betanine and isobetanine, the main color component of beet red as indicator compounds. The qualitative analysis technique consisted of clean-up of the colors with a $C_{18}$ cartridge, separation of the colors by cellulose TLC plate using acetone:3-methyl-1-butanol:distilled water (7:7:6) as a solvent system. Also, the quantitative analysis was performed using X-terra RP at wavelength 538 nm and $0.1\%$ phosphoric acid : methanol (90:10) as a solvent. The quantitative results of beet .ed were as follows:900.22$\∼$27701.60 $\mu$g/g for 60 item in nutrient supplement food, $21.95\∼713.40{\mu}g/g$ for 30 items and N.D. for 18 items in cindy, and $155.85{\∼}505.37{\mu}g/g$ for 12 items in ice creams, $43.52\∼64.75{\mu}g/g$ for 18 items and N.D. for 54 item in sauce, N.D. for 12 items in retort food.
Purpose: Since radiopharmaceuticals are intended for human administration, it is imperative that we should undergo quality control very strictly. Now almost all the PET laboratories have adopted Bacterial Endotoxin Test as the stand quality control method to monitor whether pyrogen is free or not in the product vial containing crude solution. The aim of this study is to find out the reason why false positive result is observed when using commercially available test vial. Materials and Methods: For this experiment, we used commercially available single test kit (Associates of Cape Code. Inc. USA) and we made pH samples by mixing each buffer whose pH ranges are 1.0 to 12.0. Otherwise we made Ethanol samples diluted with distilled water. After making test samples, it added 0.2 mL to the test vial. Assay mixture in the test vial was incubated in a water bath (Chang Shin Co. KOR) for 60 min at $35{\pm}2^{\circ}C$. Results: After incubation period ($60{\pm}1^{\circ}C$), we inverted the test vial about $180^{\circ}$ To know what pH and how many percentage of Ethanol (Fisher Scientific Korea. Ltd) will affect the reaction. With pH buffer, false positive result was observed at pH 1.0 to 5.0 and 7.7 to 12.6 but at pH 5.2 to.7.5, the test results show negative. It's very strange that we couldn't observe negative test result with Tris buffer at pH 8.4, 8.6, 8.8, 9.0. in other case Ethanol, the test result was seen with 5 to 10% Ethanol. But to my surprise we could see very thick gel formation with 100% Ethanol. Conclusions: In this study, we could notice that pH which is too much acidic or alkalic or high concentrated Ethanol would affect Bacterial Endotoxin Test result. As you know, LAL test is sensitive and very reliable method. Therefore, we are needed to elicit the accurate test result as possible as we can.
The conditions for formation of allophane in volcanic ash soils in Cheju Island were investigated. Soils of toposequence distributed along the sourthern slope of Mt. Halla, and the major soil groups such as dark brown soils, very dark brown soils, black soils, and brown forest soils were colleted and analyzed for Al, Fe and Si extracted with solutions of pyrophosphate and oxalate. Mean annual temperature decreased $0.8^{\circ}C$ and mean annual precipitation increased 110mm with increase elevation of 100m. Organic carbon content increased and soil pH decreased with elevation, and the formation of allophane in soils formed a climosequence. Dark brown soils widely distributed in the northern and western coastal areas, where the mean annual precipitation ranged 1,240~1,420mm and the evaporation ranged 1,290~1,320mm, contained only small amounts of allophane and Al-humus complexes. For other soils, organic carbon content, pyrophosphate extractable Al, and $Al_p/Al_o$ were inversely correlated with $pH(CaCl_2)$. Allophane content showed close relationships wlth $pH(CaCl_2)$, and inverse relationships with organic carbon content and $Al_p/Al_o$.
To evaluate the water quality in Juksancheon constructed wetlands for treating non-point source pollution, the removal rates of nutrients in water and the total amounts of T-N and T-P uptakes by water plants were investigated. Chemical characteristics of T-N and T-P in sediment were investigated. The concentrations of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), SS (Suspended Solids), T-N and T-P in inflow were 0.07~1.47, 0.60~2.65, 0.50~4.60, 1.38~6.26 and $0.08{\sim}0.32mg\;L^{-1}$, respectively. The removal rates of BOD, COD, SS, T-N, and T-P were -10, 51, 66, -3 and 5%, respectively. The maximum amount of T-N uptake by water plants in August was $368.7mg\;plant^{-1}$ in the $2^{nd}$ treatment stage by Nymphoides peltata, $1314.6mg\;plant^{-1}$ in the $3^{rd}$ treatment stage by Iris pseudacorus, $1160.4mg\;plant^{-1}$ in the $4^{th}$ treatment stage by Nymphaea tetragona GEORGI, respectively. The maximum amount of T-P uptake by water plants in August was $121.7mg\;plant^{-1}$ by Nymphoides peltata in the $2^{nd}$ treatment stage, $268.7mg\;plant^{-1}$ by Iris pseudacorus in the $3^{rd}$ treatment stage and $212.0mg\;plant^{-1}$ by Nymphaea tetragona GEORGI in the $4^{th}$ treatment stage, respectively. Organic matter contents in sediments were not different. Contents of T-N and T-P in sediments were higher in spring. Microbial biomass C:N:P ratios in sediments in spring, summer, autumn and winter were 117~140:1~4:1, 86~126:5~6:1, 68~101:2~6:1 and 47~138:2~4:1, respectively. We could conclude that Juksancheon constructed wetlands show high removal efficiencies of COD and SS. However, improvements of management in winter season should be considered to improve the removal efficiencies of pollutants.
Oyster shell has a high content of $CaCO_3$ to be used as a acidic soil amendment. To enhance productivity of barley and soil microbe in an upland soil, oyster shell and calcium-magnesium carbonate were selected as a soil amendments in this study. A field experiment was treated no treatment (hereafter, control), oyster shell lime $3.09Mg\;ha^{-1}$ and $2.38Mg\;ha^{-1}$, and calcium-magnesium carbonate $2.38Mg\;ha^{-1}$ as amount of lime requirement in silt loam soil. The yield of barley from the oyster shell lime treatment was the highest. The protein content of barley was the highest of 11.1% in the calcium-magnesium carbonate, followed by 10.7% for the control, 10.6% for the oyster shell lime $3.09Mg\;ha^{-1}$, and 10.4% for the oyster shell lime $2.38Mg\;ha^{-1}$. Soil pH value was higher than that of control in harvesting stage. In addition, the population of soil bacteria was highest in oyster shell lime $2.38Mg\;ha^{-1}$, actinomycetes was highest in calcium-magnesium $2.38Mg\;ha^{-1}$. We concluded that the oyster shell lime can be effective to restore soil nutrient and microbe balance in an upland soil.
Background. Limited ischemic tolerance of the lung has remained one of the factors that limits the expansion of pulmonary transplantation as a treatment for end-stage pulmonary disease. Numerous studies on safe long term preservation for lung transplantation has been performed for the purpose of developing ideal preservation solution with extracellular type or intracellular type solutions. In this. study, we examined the efficacy of L DG solution in lung preservation longer than 20 hours by comparison with modified Euro-Collins solution. Iwethods. Thirty-(our adult mongrel dogs were divided into two groups. Donor lungs were flushed with LPDG solution(n=9) or modified Euro-Collins(MEC) solution(n=8) and stored for 24 hours at 1$0^{\circ}C$. All donor lungs were perfused through the pulmonary arteries with solutions containing prostaglandin El and verapamil. Left canine lung allotransplantations wereperformed. Assessment(hemodynamic indices and arterial blood gas analysis) of left implanted lung was made by occluding the right pulmonary artery for ten minutes using pulmonary artery Cuff. Assessment was repeated at the interval of 30 minutes, one hour, and two hours later after reperfusion and then chest X-ray, computed tomogram and lung perfusion scan were obtained. In survival dogs follow-up studies were done with assessment with chest X-ray, computed tomogram of the chest and lung perfusion scan on 7th day postoperatively. After preservation above 20 hours, pathological examinations for ultrastructural findings on right lung were performed in each group. Results. With respect to arterial oxygen tension, LPDG group was superior to MEC but there was no statistical significance for 2 hours after reperfusion. Mean pulmonary artery pressure was less increased(p < 0.05) and cardiac output higher(p <0.05) than MEC group until 2 hours after reperfusion. After 2 hours of reperfusion, both groups showed transplanted lung function deteriorated gradually. Perfusion scan of the transplanted lung in LPDG group showed better perfusion rate in immediate post-reperfusion, 3 days and 7 days later respectively but there was no statistical significance and corelation with PaO2 and computed tomoRravhic views. In scanning electron microscopy of pulmonary artery after preservation, LPDG group relatively shows less irregular protrusion of the inner surface of endothelial cell of poulmonary artery than MEC group. Conclusions, e concluded that LPDG solution can offer safe lung preservation above 20 hours with adequate immunosuppressive therapy and prevention of the infection.
Kim, Kyeong-Su;Chae, Byung-Gon;Cho, Yong-Chan;Lee, Choon-Oh;Song, Young-Suk
The Journal of Engineering Geology
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v.17
no.1
s.50
/
pp.15-25
/
2007
In general, the life and asset casualties that occur due to landslide or slope failure in urban areas are larger than that in rural areas. In order to reduce the casualties, a slope management program is necessary to categorize slopes based on properties and to manage them systematically. The slope management system is the establishment of the data base for the geological and geotechnical factor according to slope stability, and the utilization of the data base to manage slopes. The suitable system must develop to slopes in urban area through the survey, analysis and evaluation process. Based on the above necessity, the slope management program which is applicable to slope management in an urban area has been developed at Hwangryung Mt. in Busan as a target area. The developed slope management program has various functions such as slope ID number of each slope or sub-region of a mountain, making a slope data sheet, analysis and grouping of slope stability, and establishment of a data base. The slope management program is constructed by use of GIS, and the survey, test and analysis data according to all slopes can be input and edited into the program. The program can also be utilized practically by end users due to the convenient input, edition printing, management and operation of slope data. Therefore, the slope management system has been established on the application of the developed program in Busan which is located in slope area. As the system is widely applied to other cities, the slope in urban area can be managed systematically and the slope hazards can be minimized.
An orbit propagation software for the Mars orbiting spacecraft has been developed and verified in preparations for the future Korean Mars missions. Dynamic model for Mars orbiting spacecraft has been studied, and Mars centered coordinate systems are utilized to express spacecraft state vectors. Coordinate corrections to the Mars centered coordinate system have been made to adjust the effects caused by Mars precession and nutation. After spacecraft enters Sphere of Influence (SOI) of the Mars, the spacecraft experiences various perturbation effects as it approaches to Mars. Every possible perturbation effect is considered during integrations of spacecraft state vectors. The Mars50c gravity field model and the Mars-GRAM 2001 model are used to compute perturbation effects due to Mars gravity field and Mars atmospheric drag, respectively. To compute exact locations of other planets, JPL's DE405 ephemerides are used. Phobos and Deimos's ephemeris are computed using analytical method because their informations are not released with DE405. Mars Global Surveyor's mapping orbital data are used to verify the developed propagator performances. After one Martian day propagation (12 orbital periods), the results show about maximum ${\pm}5$ meter errors, in every position state components(radial, cross-track and along-track), when compared to these from the Astrogator propagation in the Satellite Tool Kit. This result shows high reliability of the developed software which can be used to design near Mars missions for Korea, in future.
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