• 한국식품영양학회지
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• 제25권4호
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• pp.888-896
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• 2012

Considering the dearth of information regarding the medicinal properties of Luffa cylindrica, we assessed the antioxidative, antimutagenic and hyperplasia inhibitory activity of cancer cells from Luffa cylindrica extracts by employing biological and biochemical assays. Ethanol extracts of Luffa cylindrica inhibited MDA-BSA (malondialdehyde-bovine serum albumin) conjugation reaction ($66.38{\pm}2.65$), DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical production ($60.13{\pm}0.42$) and lipid peroxidation ($56.04{\pm}3.24$). In this study, Luffa cylindrica is believed to exert possible antioxidative effects. The direct and indirect antimutagenic effects of the ethanol extracts of Luffa cylindrica were examined by the Ames test using Salmonella typimurium TA98 and TA100. The inhibitory effects on indirect and direct mutagenicity shows an weak tendency, particularly in direct mutagenicity mediated by 2-nitrofluorene in Salmonella typimurium TA98 ($5.82{\pm}5.74$) and in indirect mutagenicity mediated by 2-anthramine in Salmonella typimurium TA100 ($5.76{\pm}2.15$). The ethanol extracts of Luffa cylindrica on cancer cell hyperplasia inhibitory activity via MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay exerted cytotoxic effects on Hela cells ($55.83{\pm}3.83$) and MCF-7 cells ($33.03{\pm}2.09$), which were used in this study. Based on these results, it believed that the ethanol extracts of Luffa cylindrica have antioxidative capacities as well as hyperplasia inhibitory activity of cancer cells. Furthemore, Luffa cylindrica is a candidate for the prevention and dietetic treatment of chronic diseases and for the development of functional food.

• Applied Biological Chemistry
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• 제39권1호
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• pp.67-69
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• 1996

채취시기(採取時期)에 따른 수세미오이(Luffa cylindrica L.) 수액(樹液)의 몇가지 물리적 특성(特性)과 무기성분 함량(含量)을 조사하였다. 온실(溫室)(green house)과 노지(露地)(open culturing)에서 재배한 수세미오이의 줄기로 부터 수액(樹液)을 시기별로 채취(採取)하여 분석(分析)한 결과 명도(L), 적도(a), 황색도(b), pH 및 탁도는 시료간에 별 차이가 없었다. pH는 전체적으로 약알칼리성을 나타내었고 재배기간이 경과함에 따라 약간씩 감소(減少)하는 경향(傾向)이었다. 무기성분은 총 8개 항목을 조사한 결과(結果) K, Ca, Mg이 주요성분(主要成分)이었으며, Na, Zn, Fe는 미량(微量)함유되어 있었다. Cu는 노지재배에서는 검출(檢出)되지 않았으며 Mn은 모든 시료에서 검출되지 않았다.

• Natural Product Sciences
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• 제25권4호
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• pp.341-347
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• 2019

Luffa cylindrica (LC) is a very fast-growing climber and its fruit have been considered as agricultural wastes. We conducted to check the comparative qualities of ethanol solvent extraction (LCE) and supercritical carbon dioxide extraction (LCS) of L. cylindrica fruit and seed. LCS had higher antioxidant and polyphenol contents than LCE. LCS were significantly increased peroxisome proliferator-activated receptor (PPAR)-a and involucrin expression as epidermal differentiation marker in 3D skin equivalent model. LCS also showed antimicrobial activity against Staphylococcus aureus, a causative bacteria in atopic dermatitis. In addition, LCS inhibited the adipocyte differentiation of 3T3-L1 cells. When treated with the extract at a concentration of 100 ㎍/mL, the Wnt/β-catenin pathway reporter luciferase activity of HEK 293-TOP cells was increased approximately by 2-folds compared to that of the untreated control group. These results indicate that L. cylindrica supercritical carbon dioxide extract may serve as a cosmeceutical for improving skin barrier function and the treatment of obesity.

• The Plant Pathology Journal
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• 제22권3호
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• pp.211-214
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• 2006

In 2004 and 2005, Phytophthora rot on Luffa cylindrica which had not been reported in Korea occurred in the experimental field at Gyeongsangnam-do Agricultural Research and Extension Services. The disease initiated on leaves and fruits of the plant with small watersoaked dark brown spots and progressed rapidly. The causal pathogen isolated from diseased tissues was identified as a Phytophthora sp. because of aseptate mycelia and zoospores released directly from sporangia. The fungus grew well on PDA and 10% V-8 juice agar showing an arachnoid or rosaceous colony pattern. Sporangia formed abundantly in water and were conspicuously papillate, noncaducous, ovoid to globose, and sized $26\sim62\times19\sim38{\mu}m$. The fungus was heterothallic as producing sexual reproduction structures only when mated with only A2 standard mating type strain. Oogonia and oospores were spherical, smooth walled, and measured as $20\sim28{\mu}m\;and\;16\sim24{\mu}m$, respectively. Oospores were aplerotic and antheridia were amphigynous, unicellula and spherical. Chlamydospores were globose and $20\sim38{\mu}m$ in diameter. Optimum temperature for growth was around $28\sim30^{\circ}C$. The fungus caused similar symptoms on artificially inoculated plant and could be re-isolated thereby proving Koch's postulation. Based on the mycological criteria investigated in this study, the causal fungus of Luffa sylindrica rot was identified as Phytophthora nicotianae. This is the first report of Phytophthora rot of Luffa cylindrica caused by P. nicotianae in Korea.

• 식물병연구
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• 제22권2호
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• pp.107-110
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• 2016

In October 2014, an occurrence of gray mold was observed on young fruits of sponge gourd (Luffa cylindrica) in Sachunmun, Gangneung, South Korea. Symptoms included abundant mycelia growth with gray conidia on young fruits and finally rotting the fruits. The fungus was isolated from symptomatic fruits and its pathogenicity was confirmed. Based on the morphological features and sequence analysis of ITS-5.8S rDNA, G3PDH, HSP60, and RPB2 genes, the pathogen was identified as Botrytis cinerea Pers. This is the first report of gray mold caused by B. cinerea on L. cylindrica in Korea.

• 생약학회지
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• 제22권4호
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• pp.249-251
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• 1991

The cytotoxic and antibacterial activities of the leaf, the stem and the seed extracts of Luffa cylindrica were evaluated against L1210 cells and Streptococcus mutans OMZ176, respectively. Among hexane, ether, butanol and water fractions, the ether fraction demonstrated the most potent cytotoxic activity, and the $ED_{50}$ values of the ether extract from the leaf, the stem and the seed were 3.5, 3.7 and $13.7{\mu}g/ml$, respectively. Meanwhile, the other fractions showed negligible effect. Separately, it was found that the antibacterial activity of the respective fraction from three parts was insignificant.

• 생명과학회지
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• 제18권7호
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• pp.1019-1022
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• 2008

박과 작물 4종(오이, 수박, 참외, 수세미)에 대한 핵형분석 연구를 수행하였다. 염색체 조성은 모두 이배체로 염색체 수와 형태적 특징에 있어서 종에 따라 차이를 나타냈다. 오이의 핵형은 2n=14=14m, 수박은 2n=22=22m으로 2번 염색체가 부수체를 지니며, 참외는 2n=24=18m+6sm으로 7번 염색체가 부수체를 지니고 있었다. 수세미는 2n=26=26m으로 1번 염색체가 부수체를 지니고 있었다. 핵형분석 결과 종에 따라 특징적인 염색체 수와 조정을 나타내었으며 종간 게놈 구성의 정보에 따른 박과 작물 육종화 작업의 기초 자료를 제공할 수 있으리라 본다.

• 한국자원식물학회지
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• 제22권6호
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• pp.540-545
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• 2009

Aloe (Aloe vera L.), cucumber (Cucumis sativus L. var. tuberculatus Gabaj.) and sponge.gourd (Luffa cylindrica L.) are well utilized in Asian countries as traditional medicines and cosmetics, or foods. And we carried out an experiment to see if there are some specific ratios among trace metals on the plants. Though the metal concentrations varied significantly depending on the sampling periods, aloe gel is high in iron (Fe) and calcium (Ca). Cucumber fruit juice is also high in iron (Fe) and potassium (K). Sponge.gourd sap contains a high amount of zinc (Zn) and calcium (Ca). The uses of the previous 3 plant parts are considered to have some relations to their different ratios and of their trace metals.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.21-28
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• 2010

The objective of the present investigation is to evaluate the antimicrobial potency of the terpenoid fractions isolated from Luffa cylindrica seeds against various pathogenic microbes. The seeds were powdered and extracted with methanol in soxhlet appratus based on phytochemical screening. Three terpenoid components were isolated by column chromatography and identified by thin layer chromatography and chemical analysis which were designated as ${LCSF_4}^*$, ${LCSF_6}^*$ & ${LCSF_8}^*$ respectively. Disc diffusion method was employed to determine the antimicrobial effectiveness of test compounds I, II and III $({LCSF_4}^*,\;{LCSF_6}^*\;&\;{LCSF_8}^*)$ against 6 microbial species viz., Staphylococcus (S.) aureus, Bacillus (B.) subtilis, Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, Candida (C.) albicans and Aspergillus niger. The disc was saturated with $100{\mu}l$ of each compound, allowed to dry and introduced on the upper layer of seeded agar plate. The plates were incubated overnight at $37^{\circ}C$. Microbial growth was determined by measuring the zonal inhibition diameters. Compound I showed maximum potency against gram positive S. aureus (21 mm) in comparison with standard ciprofloxacin (38 mm), whereas the same compound was completely devoid of activity against both the fungi tested. Compound II was found to be highly sensitive against both the gram negative E. coli (20 mm) and P. aeruginosa (22 mm). Compound II was found to exhibit maximum potency against the fungi C. albicans (15 mm) and A. niger (20 mm). Compound III was found to be very effective against both the gram positive S. aureus (20 mm) and B. subtilis (15 mm) respectively.

• Applied Biological Chemistry
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• 제34권4호
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• pp.366-372
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• 1991

관행법에 의해 재배된 수세미외의 잎, 줄기, 미숙과즙, 종실, 수액 등 각 부위에 함유된 휘발성 정유성분, 탄화수소, 유기산 및 비휘발성 지방산을 추출, 분리하였다. 150여개의 분리성분중 37개 정유 성분을 확인한 후 각 부위별로 정량하였다. 또한, 26종의 탄화수소, 5종의 유기산 및 6종의 지방산도 분리정량하여 부위별 분포와 함량을 비교하였다.