• 대한화장품학회지
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• 제46권3호
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• pp.243-252
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• 2020

본 연구에서는 Fervidobacterium islandicum AW-1를 이용하여 극성 저분자 케라틴 펩타이드를 생산하고, 피부 탄력과 관련한 인자를 확인하여, 화장품 원료로서 극성 저분자 케라틴 펩타이드의 가능성을 확인하였다. 인체섬유아세포에 극성 저분자 케라틴 펩타이드를 농도에 따라 세포독성 및 콜라겐 합성능을 확인한 결과, 세포 독성은 나타나지 않았고, 인체섬유아세포 내 콜라겐 합성을 증가시키는 것을 확인하였다. 극성 저분자 케라틴 펩타이드를 함유한 마스크팩을 만들어, 22 명의 건강한 성인 피험자를 대상으로 4 주 동안 시험제품을 사용한 결과, 피부 탄력 및 피부 비틀림 탄력 개선, 수분량 증가, 피부색 개선에서 통계적으로 유의한 효과를 나타냈다. 이를 통해 극성 저분자 케라틴 펩타이드는 피부 탄력 개선에 도움을 주는 화장품 원료로 사용할 수 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제34권2호
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• pp.415-424
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• 2024

This study reveals that low-molecular-weight collagen peptide (LMWCP) can stimulate the differentiation and the mineralization of MC3T3-E1 cells in vitro and attenuate the bone remodeling process in ovariectomized (OVX) Sprague-Dawley rats in vivo. Moreover, the assessed LMWCP increased the activity of alkaline phosphatase (ALP), synthesis of collagen, and mineralization in MC3T3-E1 cells. Additionally, mRNA levels of bone metabolism-related factors such as the collagen type I alpha 1 chain, osteocalcin (OCN), osterix, bone sialoprotein, and the Runt family-associated transcription factor 2 were increased in cells treated with 1,000 ㎍/ml of LMWCP. Furthermore, we demonstrated that critical bone morphometric parameters exhibited significant differences between the LMWCP (400 mg/kg)-receiving and vehicle-treated rat groups. Moreover, the expression of type I collagen and the activity of ALP were found to be higher in both the femur and lumbar vertebrae of OVX rats treated with LMWCP. Finally, the administration of LMWCP managed to alleviate osteogenic parameters such as the ALP activity and the levels of the bone alkaline phosphatase, the OCN, and the procollagen type 1 N-terminal propeptide in OVX rats. Thus, our findings suggest that LMWCP is a promising candidate for the development of food-based prevention strategies against osteoporosis.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.81-84
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• 2021

Collagen, which accounts for one-third of human protein, is reduced due to human aging, and much attention is focused on making collagen into food to prevent such aging. Gel permeation chromatography with Reflective Index (RI) detection (GPC/RI) was chosen as the most suitable instrument to confirm molecular weight distribution, and we explored the use of this technique for analysis of collagen peptide molecular sizes and distributions. Data reliability was verified by matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) mass spectrometric analysis. The data were considered meaningful for comparative analysis of molecular weight distribution patterns.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1401-1408
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• 2021

This study examined whether the oral administration of low-molecular-weight collagen peptide (LMCP) containing 3% Gly-Pro-Hyp with >15% tripeptide (Gly-X-Y) content could ameliorate osteoarthritis (OA) progression using a rabbit anterior cruciate ligament transection (ACLT) model of induced OA and chondrocytes isolated from a patient with OA. Oral LMCP administration (100 or 200 mg/kg/day) for 12 weeks ameliorated cartilage damage and reduced the loss of proteoglycan compared to the findings in the ACLT control group, resulting in dose-dependent (p < 0.05) improvements of the OARSI score in hematoxylin & eosin (H&E) and Safranin O staining. In micro-computed tomography analysis, LMCP also significantly (p < 0.05) suppressed the deterioration of the microstructure in tibial subchondral bone during OA progression. The elevation of IL-1β and IL-6 concentrations in synovial fluid following OA induction was dose-dependently (p < 0.05) reduced by LMCP treatment. Furthermore, immunohistochemistry illustrated that LMCP significantly (p < 0.05) upregulated type II collagen and downregulated matrix metalloproteinase-13 in cartilage tissue. Consistent with the in vivo results, LMCP significantly (p < 0.05) increased the mRNA expression of COL2A1 and ACAN in chondrocytes isolated from a patient with OA regardless of the conditions for IL-1β induction. These findings suggest that LMCP has potential as a therapeutic treatment for OA that stimulates cartilage regeneration.

• 대한화장품학회지
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• 제38권4호
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• pp.321-326
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• 2012

최근에는 콜라겐 펩타이드가 항상화효과를 통해 피부의 재생을 촉진시키는 효과를 한다는 연구가 보고되고 있다. 본 구는 저분자 경구 콜라겐 펩타이드가 1550 nm fractional photothermolysis 레이저 치료 후 피부재생에 미치는 영향을 알아보고자 한다. 본 실험에 참여한 환자는 총 10명으로 실험군과 대조군 각 5명씩 무작위배정되었다. 대조군과 실험군 모두 동일하게 fractional photothermolysis 레이저를 시술받았다. 실험군에서는 경구 콜라겐 펩타이드를 하루에 1,000 mg씩 총 8주간 복용하였다. 치료 전후 모든 환자에서 피부의 탄성도, 경표피수분손실도, 홍반지수를 측정하였다. 피부탄성도는 실험군에서 대조군에 비해 통계적으로 유의하게 피부의 탄력성이 증가하였음을 확인하였다(p < 0.05). 경표피수분손실도는 두군간에 유의한 차이가 없었고, 홍반지수는 실험군에서 더 빨리 감소하는 결과를 보였다. 환자의 주관적 만족도평가에서 시술 4주 후 환자의 만족도는 실험군에서 평균 2.0점, 대조군에서 1.2점으로 나타났다. 저분자 콜라겐 펩타이드는 1550 nm fractional photothermolysis 레이저 치료 후 피부회복 촉진에 도움이 된다.

• Journal of Applied Biological Chemistry
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• 제62권1호
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• pp.101-107
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• 2019

The objectives of this study were to evaluate the anti-aging effects and investigate the effect of simulated gastrointestinal (GI) digestion on the anti-aging properties and intestinal permeation of the potential fish collagen hydrolysates (FCH). Therefore, procollagen synthesis, matrix metalloproteinase-1 (MMP-1) production, and Caco-2 cell permeability were analyzed before and after in vitro digestion for FCHs, low-molecular weight fractions (<1 kDa), and high molecular weight fractions (>1 kDa). After being subjected to GI digestion, the level of MMP-1 inhibition was maintained, although the procollagen production was significantly (>20%) lower with all samples. Also, the digested FCHs and their <1 kDa fraction yielded 9.1 and 13.8% increased peptide transport, respectively, compared to undigested samples. Based on the effective intestinal permeation and high digestive enzyme stability, the <1 kDa fraction of FCHs is a potential bioactive material suitable for anti-aging applications in the food and cosmetics industries.

• 한국응용과학기술학회지
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• 제30권3호
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• pp.560-566
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• 2013

In this research we extracted water-soluble collagen peptide from flatfish skin and compared it with commercially available collagen peptide extracted from Tilapia scale currently placed on the market in the aspect of physiochemical property. The physical property and nutritional components of FSCP appeared almost similarly to those of TSCP, and also in calorie, FSCP marking 3.82 Kcal showed no differences from TSCP marking 3.84 Kcal. As for forming amino acids, in aspartic acid, serine, histidine, tyrosine, methionine, FSCP had higher content than TSCP, but in OH-proline, proline and alanine FSCP had lower content than TSCP. Especially the content of essential amino acids of FSCP marked 22.74% with a higher content compared with 13.64% of TSCP. In the distribution of molecular weight FSCP with 1,000 Da showed comparatively low compared with TSCP, and in emulsion property and stability FSCP and TSCP showed similar excellent trend.

• 한국재료학회지
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• 제34권4호
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• pp.215-222
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• 2024

Because collagen is inherently piezoelectric, research is being actively conducted to utilize it to harvest energy. In this study, a collagen solution was prepared using edible low-molecular-weight peptide collagen powder, and collagen films were fabricated using a dip coating method. The collagen films prepared by dip coating showed a smooth surface without defects such as pinholes or cracks. Dehydrothermal treatment of the collagen films was performed to induce a stable molecular structure through cross-linking. The collagen film subjected to dehydrothermal treatment at 110 ℃ for 24 h showed a thickness reduction rate of 19 %. Analysis of the collagen films showed that the crystallinity of the collagen film improved by about 7.9 % after dehydrothermal treatment. A collagen film-based piezoelectric nanogenerator showed output characteristics of approximately 13.7 V and 1.4 ㎂ in a pressure test of 120 N. The generator showed a maximum power density of about 2.91 mW/m² and an output voltage of about 8~19 V during various human body movements such as finger tapping. The collagen film-based piezoelectric generator showed improved output performance with improved crystallinity and piezoelectricity after dehydrothermal treatment.

• 한국식품저장유통학회지
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• 제16권6호
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• pp.938-945
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• 2009

고지혈증 흰쥐에 있어서, 숭어(Mugil cephalus) 비늘에서 추출 제조된 저분자 콜라겐 펩타이드 추출물의 급여가 혈청 지질개선 효과 및 지질대사 이상 등에 관여하는 지질성분 및 효소의 활성 변동을 검토하기 위하여 Sprague Dawley계 흰쥐 수컷을 사용하여 본 실험을 행하였다. 기본식이만을 급여한 대조군인 BG군을 비롯한 고지혈증 유발군(CW군), 고지혈증 유발에 콜라겐 펩타이드를 섭취시킨 군(CCP군)을 5주간 실험사육하였다. 혈청 총 콜레스테롤, 동맥경화지수, LDL-콜레스테롤, 유리 콜레스테롤, 콜레스테롤 에스테르 비, 중성지방 및 인지질 농도 및 혈당 농도 등은 저분자 콜라겐 펩타이드 추출물을 섭취시킨 군(CCP군)에서 농도가 감소되는 것으로 나타났다. 한편, HDL-콜레스테롤 및 총 콜레스테롤에 대한 HDL-콜레스테롤 비 등은 콜라겐 펩타이드 급여(CCP군)에 의해서 증가되는 것으로 나타났다. 혈청 중 alkaline phosphatase (ALP) 및 aminotransferase (AST, ALT)의 활성은 고지혈증을 유도 시킨 후 콜라겐 펩타이드를 급여시킴으로써 감소되는 것으로 나타났다. 이상과 같은 결과들을 미루어 볼 때, 저분자 콜라겐 펩타이드 추출물이 고지혈증 유발 흰쥐의 혈청 지질대사 이상 등에서 오는 고지혈증의 예방 및 개선에 효과가 있을 것으로 추정된다.

• 공업화학
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• 제24권1호
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• pp.18-23
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• 2013

본 연구는 가자미껍질에서 콜라겐 펩타이드 파우더(FSCP)를 제조하여 시판 틸라피아비늘 유래 콜라겐 펩타이드 파우더 (TSCP)와 이화학적 특성을 비교 검토하였다. FSCP의 물리적인 특성 및 영양성분은 TSCP와 유사하게 나타났으며, 열량에 있어서도 FSCP는 3.82 kcal로 TSCP의 3.84 kcal와 차이가 없는 것으로 나타났다. 아미노산 조성은 FSCP가 TSCP보다 aspartic acid, serine, histidine, tyrosine, methionine의 경우 높았으나, hydroxyproline, proline, alanine은 오히려 낮았다. 특히 필수아미노산 함량은 FSCP가 22.74%로, TSCP의 13.64%보다 높았다. 분자량 분포는 FSCP가 1000 Da으로, TSCP에 비하여 비교적 낮은 분포를 보이고, 유화성 및 유화안정성은 FSCP와 TSCP가 유사한 경향으로 우수하였다.