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http://dx.doi.org/10.3839/jabc.2019.015

Anti-aging potential of fish collagen hydrolysates subjected to simulated gastrointestinal digestion and Caco-2 cell permeation  

Je, Hyun Jeong (Department of Food and Nutrition, Hanyang University)
Han, Yoo Kyung (Department of Food and Nutrition, Hanyang University)
Lee, Hyeon Gyu (Department of Food and Nutrition, Hanyang University)
Bae, In Young (Department of Food and Nutrition, Far East University)
Publication Information
Journal of Applied Biological Chemistry / v.62, no.1, 2019 , pp. 101-107 More about this Journal
Abstract
The objectives of this study were to evaluate the anti-aging effects and investigate the effect of simulated gastrointestinal (GI) digestion on the anti-aging properties and intestinal permeation of the potential fish collagen hydrolysates (FCH). Therefore, procollagen synthesis, matrix metalloproteinase-1 (MMP-1) production, and Caco-2 cell permeability were analyzed before and after in vitro digestion for FCHs, low-molecular weight fractions (<1 kDa), and high molecular weight fractions (>1 kDa). After being subjected to GI digestion, the level of MMP-1 inhibition was maintained, although the procollagen production was significantly (>20%) lower with all samples. Also, the digested FCHs and their <1 kDa fraction yielded 9.1 and 13.8% increased peptide transport, respectively, compared to undigested samples. Based on the effective intestinal permeation and high digestive enzyme stability, the <1 kDa fraction of FCHs is a potential bioactive material suitable for anti-aging applications in the food and cosmetics industries.
Keywords
Anti-aging peptide; Cellular permeability; Collagen synthesis Fish collagen hydrolysate; Matrix metalloproteinase-1 inhibition;
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