• Food Science and Preservation
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• v.14 no.6
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• pp.688-694
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• 2007

To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.

• Journal of the Korean Institute of Rural Architecture
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• v.25 no.3
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• pp.9-16
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• 2023

This study is an attempt to organize the historical records of the Hanok Church, which played a major role in the history of Korean Protestantism. It was confirmed that research on the theme of Hanok Church was not actively conducted. Through this study, we would like to study the theoretical consideration of the study of Hanok Church and the value of Hanok Church. Based on these results, the purpose was to serve as the basis for theoretical data on the theory of Hanok Church and to actively conduct related research. In addition, by systematizing the cultural values of the Hanok Church, basic information can be provided to establish future cultural values and business utilization plans as modern cultural heritage. Therefore, it is necessary to expand various consultative bodies to preserve Hanok churches around the region and churches and promote various projects to improve the environment for the preservation of Hanok churches. Lastly, I think efforts for long-term preservation and related research of the Hanok Church are continuously needed.

• Food Science and Preservation
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• v.31 no.3
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• pp.346-359
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• 2024

Although ovalbumin makes up 54% of the total egg white proteins, individual protein usage is rare. The primary applications of ovalbumin in the food industry relate to other proteins, such as whole egg whites. Ovalbumin has remarkable functional properties, such as those of gelation, foaming, and emulsification, which are crucial in the processing of food, however, its application as a standalone functional protein is severely constrained due to separation issues. In recent years, new methodologies for the large-scale separation of ovalbumin have emerged. Meantime, ovalbumin was identified as a good source to produce bioactive peptides with a variety of functional properties, including antibacterial, antioxidant, and angiotensin-converting-enzyme inhibitory actions, according to research. Newly discovered bioactive peptides from ovalbumin can be used in the food sector in addition to their well-known functional properties to create health-promoting products. Benefits extend beyond the food business to numerous other sectors, such as the pharmaceutical and cosmetic industries. Consequently, a gap between the existing and prospective future uses is found. The main goals of this study were to determine some possible factors for the long-term neglect of the major protein and to determine the growing potential for applications of ovalbumin and peptides.

• Animal Bioscience
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• v.37 no.2
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• pp.203-209
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• 2024

Objective: This study aimed to assess the impact of the dilution ratio of Tris diluent, storage at 0℃, and long-distance transportation on the spermatozoa of Simmental cattle. It also validated the feasibility of the regional distribution of fresh semen. Methods: In experiment 1, semen was diluted at four dilution ratios (1:6, 1:9, 1:12, and 1:15) to determine the optimal dilution ratio of Tris diluent. In experiment 2, we assessed sperm viability, progressive motility (objectively assessed by computer-assisted sperm analyzer), and acrosome intactness in Tris dilutions kept at constant 0℃ for 1, 3, 6, 9, and 12 days. We compared them to Tianshan livestock dilutions (Commercial diluent). In experiment 3, semen was diluted using Tris diluent, and sperm quality was measured before and after long-distance transport. Artificial insemination of 177 Simmental heifers compared to 156 using Tianshan Livestock dilution. Results: The outcomes demonstrated that 1:9 was the ideal Tris diluent dilution ratio. The sperm viability, Progressive Motility, and acrosome integrity of both Tris and Tianshan dilutions preserved at 0℃ gradually decreased over time. sperm viability was above 50% for both dilutions on d 9, with a flat rate of decline. The decrease in acrosome integrity rate was faster for Tianshan livestock dilutions than for Tris dilutions when stored at 0℃ for 1 to 6 days. There was no significant difference (p>0.05) in sperm viability between semen preserved in Tris diluent after long-distance transportation and semen preserved in resting condition. The conception rates for Tris dilution and Tianshan livestock dilution were 49.15% and 46.15% respectively, with no significant difference (p>0.05). Conclusion: This shows that Tris diluent is a good long-term protectant. It has been observed that fresh semen can be successfully preserved for long-distance transport when stored under 0℃ conditions. Additionally, it is feasible to distribute semen regionally.

• Journal of Sericultural and Entomological Science
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• v.46 no.2
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• pp.65-71
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• 2004

Long term preservation experiment through refrigeration was conducted for 2 year on 300 lines of silkworm races preserved, as one method for the development of long term safe preservation technique. Experiment with 6 treatments was conducted for 680 days from July 1 st 2000 to May 1st 2002. Embryonic development was conducted to each treatment. There are no differences among treatments and races in 400 days preservation, the stage of whole embryonic development was Eul B and condition of eggs was good. In 650 days preservation experiment, differences were reveled among races and treatment, the level of whole embryonic development was Byeong A and condition of eggs was good. The order of embryonic development is European races >Tropical, Korean races >Japanese, Chinese races, thus European races showed fast embryonic development. Control(treatment A) and treatment C showed faster development than other treatments. And treatment D and F showed stable individual stage among all treatments. The test of shape characteristics and embryo which were conducted in hatching period showed 61% of high line succession possibility in average of 6 treatments. But treatment A and B showed no hatching, 3 lines of treatment C, 48 lines of treatment D, 1 line of treatment E, and 29 lines of treatment F showed slow development. And treatments D and F which showed stable embryo condition had highest possibility. The two treatments D and F showed good result among six treatments, and the preservation period of treatment D and F are 235 days and 310 days, and exposure period in $-2.5^{\circ}C$...was longer than other treatments. Numbers of hatched lines of treatment D and F are 48 and 29, and occupied 15.6% and 9.4% of all tested lines, respectively. Average hatching ratio of treatment D and F were 54.% and 71.6%, and average dead egg ratio were 12.6% and 2.4%, respectively. These results show that average ratio of hatching dead eggs in treatment D and F are higher than general line. Thus reconsideration of hatching condition on treatment D and F is needed.

• Journal of Korean Neurosurgical Society
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• v.40 no.5
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• pp.336-341
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• 2006

Objective : The authors conducted a retrospective study to evaluate the preservation rates of serviceable hearing and to determine its prognostic factors after gamma knife stereotactic radiosurgery[GK SRS] in the patient with vestibular schwannomas. Methods : Between December 1997 and March 2005, 54 patients with a sporadic vestibular schwannoma and serviceable hearing [Gardner Robertson grade I-II] were enrolled in this study. Electronic database of medical records and radiological examinations before and after GK SRS were investigated to the last follow up. The mean marginal dose was $12.3{\pm}0.7Gy$. The mean maximum dose delivered to the tumor center was 24.7Gy [$22{\sim}30Gy$]. The median tumor volume was 2cc [$0.1{\sim}9.1cc$]. The median follow-up period of magnetic resonance[MR] imaging was 31 months [$6{\sim}99\;months$], and the mean follow-up period of audiometry was 24 months [$4{\sim}70\;months$]. Results : The tumor control rate was 100% in the patients with the follow up period more than 2 years. The trigeminal and facial nerve preservation rates were 98% and 100%, respectively. Twenty-eight [52%] of the 54 patients preserved serviceable hearing and 16 [30%] patients retained their pre-GK G-R grade level after GK SRS. In the univariate and multivariate analysis, there was no significant prognostic factor in preservation of the serviceable hearing. Conclusion : The hearing preservation rate is still unsatisfactory compared with the results of other cranial nerve preservation and tumor control in the treatment of vestibular schwannoma by GK SRS. More sophisticated strategy during and after GK SRS is necessary to improve long-term hearing preservation.

• The Journal of the Korean dental association
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• v.56 no.10
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• pp.572-580
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• 2018

Adequate access cavity is the key to achieving endodontic success. The aims of the access cavity can be considered as follows: 1) Creation of a smooth unimpeded pathway for instruments to canal orifices 2) Removal of the entire roof of the pulp chamber in order to inspect the pulp floor, 3) Preservation of natural tooth substance consistent with the above. Recently, contracted endodontic cavities based on minimally invasive endodontics has introduced. This has the benefit of preserving the pericervical dentin more than traditional access cavity with achieving long-term success. However, some studies reported controversial results regarding root canal detection, instrumentation efficacy (noninstrumented canal area, hard tissue debris accumulation, canal transportation, and centering ratio) as well as fracture resistance. Therefore, further studies are required for accepting contracted access cavity, and modified form of traditional and contracted access cavity could be considered.

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.2
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• pp.110-117
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• 1998

It is essential to get full social supports of our national parks for the achievement of long-term goals of our national parks. Thus the main objective of this paper is to identify major perception types of interest groups of our national parks. The research method is greatly indebted to Hofer. Two stages of questionnaire surveys were carried out to identify perception types related to national parks and the statstical analysis of perception types. The findings of this study are as follows. First, eight types of perception on national parks were selected through the statistical analysis of ten preliminary perception types; nature preservation, appreciation of scenic beauty, culture, recreation, research, education, ethics, and economy. Second, three or four phrases relevant to eight perception types were identified. Third, it could be concluded that perception types identified through this research are significantly different from those commonly perceived on national parks and natural landscapes in western countries.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.36 no.1
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• pp.75-80
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• 2004

This research was peformed to manufacture a shroud using hanji and to investigate the characteristics of the hanji shroud manufactured. A hanji shroud were manufactured by considering the designs of the traditional costumes of shroud. The vast fibers were used for the manufacture of hanji. The shroud manufactured could keep the natural feeling of hanji. The shroud showed the feasibility in sewing compared to textiles. However, fine needlework was required due to the poor appearance of small holes made by changing of sewing line. Since the hanji shroud has excellent ability in preservation for a long-term period and economical advantages compared to the shroud currently made by hemp and cotton cloth, the demand of the hanji shroud may be increased in the near future.

• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.56-60
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• 1999

Sexual reproduction is an essential process in the propagation of flowering plants. Recent advances in plant cell biology and biotechnology have brought new and powerful methodologies to investigate and manipulate the reproductive processes of angiosperms including agronomically important crop plants. Successful cryopreservation of maize, rye and triticale pollen and young embryos of microspore-and zygote-origine contributes to long term preservation of important plant germ-lines in gene banks. Discovering morphogenetic characteristics of the different developmental pathways taking place in wheat and maize androgenesis in vitro helps to influence the procedure to produce genetically and phenotipically stable homozygous doubled haploid plants for breeding purposes. Detailed ultrastructural and cell-biological studies on the developmental sequences of male and female gametophyte development in wheat, experimental protocols developed to isolate and micromanipulate egg cell protoplasts, make it possible to use plant gametes and the sexual route itself to produce genetically improved organisms. Plant gametes can become useful tools for crop improvement in the near future. Recent achievements by our laboratory in this field are reviewed in the present paper