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Comparison of Expression Pattern of Housekeeping Genes in Mice fed Genetically Modified Rice  

Lee, Dong-Yeob (Department of Food Science & Biotechnology, Kyungpook National University)
Heo, Jin-Chul (Food & Bio-industry Research Institute, Kyungpook National University)
Lee, Kyu-Hyun (Department of Food Science & Biotechnology, Kyungpook National University)
Kim, Dong-Ho (Department of Food Science & Biotechnology, Kyungpook National University)
U, Sang-Uk (Department of Food Science & Biotechnology, Kyungpook National University)
Cho, Hyun-Suk (National Institute of Agricultural Biotechnology, RDA)
Lee, Sang-Han (Department of Food Science & Biotechnology, Kyungpook National University)
Publication Information
Food Science and Preservation / v.14, no.6, 2007 , pp. 688-694 More about this Journal
Abstract
To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.
Keywords
Genetically modified; housekeeping gene; rice; gene transfer;
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