• KSTLE International Journal
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• 제7권1호
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• pp.10-13
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• 2006

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.

• KIEE International Transactions on Electrophysics and Applications
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• 제4C권4호
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• pp.160-164
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• 2004

In this study, we demonstrate for the first time a bio-inspired Cell-Microsystem to manipulate and detect living cells. Cultured retinal pigment epithelial cell line (ARPE-19) was directed to grow in a pre-defined Cell-Microsystem. The three-dimensional micropillars of 5 ${\mu}{\textrm}{m}$ in height and diameter of the Cell-Microsystem were fabricated. Inhibited DNA synthesis and transformed cell morphology were observed throughout the culture period. The demonstration of manipulating and detecting living cells by the surface topography is a new approach, and it will be very useful for the future design of cell-based biosensors and bioactuators.

• 한국노년학
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• 제38권3호
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• pp.467-480
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• 2018

고령화의 가속화에 따라 독거노인들의 수도 증가하고 있다. 독거노인들은 동거노인들에 비하여 사회적 지지가 낮고 사회적으로 더 고립되어 우울 유병율이 더욱 높다. 본 연구는 기술의 발달과 정보화의 진전에 따라 노인들의 휴대폰 보유율이 높아지면서 휴대폰이 노인들의 사회적 지지와 연결의 수단이 될 수 있을 것으로 보고, 2014년 노인실태조사 자료를 활용하여 독거노인들의 휴대폰 활용정도가 우울에 미치는 영향을 분석하였다. 총 2,493명의 65세 이상 독거노인을 분석 대상으로 하였으며 휴대폰 활용 정도는 (1) 휴대폰 없음, (2) 통화기능만 이용, (3) 통화/문자기능 이용, (4) 통화/문자와 정보검색까지 이용으로 나누어 구분하였고 공변량은 성별, 나이, 교육수준, 건강상태, 경제수준, 사회활동으로 하였다. 연구 결과 81.5%의 독거노인이 휴대폰을 가지고 있는 것으로 나타났다. 휴대폰을 가진 독거노인들 중 정보검색까지 하는 군은 3.5%를 차지하였으며, 정보검색까지 하는 군이 휴대폰이 없는 군에 비하여 우울의 가능성이 더 낮은 것으로 나타났다(OR=0.064, 95% CI=0.018-0.227). 이상 결과를 통해 독거노인들이 통화와 문자기능뿐만이 아니라 정보검색까지 이용함으로써 사회적 연결정도가 높아져 우울의 정도가 낮아지는 것으로 추측 해 볼 수 있다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.169.1-169.1
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• 2003

Recently development of cell-based assay systems which are useful in molecular cell biology and drug discovery attracts significant attention. Here, we introduce a new technologies for monitoring enzyme activity and its inhibition inside living cells. Among various enzymes, proteases are important targets for studying various biological and disease-related processes such as viral infections, apoptosis and Alzheimer's disease. In this study, a sensitive cell-based protease detection system that enables direct fluorescence detection of a target protease and its inhibition inside living cells is introduced. (omitted)

• 한국잠사곤충학회지
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• 제40권2호
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• pp.91-96
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• 1998

In relation to cold acclimation, this experiment was carried out to understand the changes of the cortical cells in the living barks of the mulberry during wintering period. The living barks of three mulberry varieties(Kaeryangppong, Shinilppong and Yongcheonppong) were sampled from December, 1995 to March, 1996. The result of this experiment was summarized as follows. The cortical cells in the living barks of the mulberry in December were filled with small vacuoles. Plastids and mitochondrias were located near the nucleus. At this time, almost all starch granules disappeared from the plastids. In January and February, mitochondria, palstids and microbodys of the cortical cell were observed. As increasing temperature from March, dictysomes and polysomes were sparse. Again, starch granules disappeared were observed in the plastids. From the above result. starch granules in plastide of the cortical cell of the mulberry disappeared during cold acclimation stage. After late January, Proplastid was observed in the cortical cell and the ultrastructures of cortical cell were actively changed.

• Archives of Plastic Surgery
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• 제32권3호
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• pp.357-362
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• 2005

The aim of this study is to elucidate the effect of keratinase on epidermis of rat skin. Twenty-five male Sprague-Dolly rats were used. The hair on the back were removed and $2{\times}2cm$ area was marked. The rats were divided five groups; 1) Control group(Co), 2) Cleansing gel group(Cl), 3) Cleansing gel+keratinase group, 4) Exfoliant gel group(Ex), and 5) Exfoliant gel+ keratinase group(Ex+K). The solutions were applied to the back area twice a day for five days. On fifth day, the skins were harvested, fixed and prepared for histologic sections. The thickness of keratin layer, living epidermis, dermis, and cell layer number of living epidermis were measured. In the group containing keratinase(Cl+K, Ex+K), the thickness of keratin layer and living layer were thinner than other groups. However, there were no significant differences of the cell layer number of living epidermis and thickness of the dermis among the five groups. We think the keratinase may have the effect thinning the keratin layer as well as the thickness of living epidermis, without effecting the living cell and dermal component. The keratinase containing soap may be of benefit to remove the excess keratin layers in human.

• 한국식품영양과학회지
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• 제14권2호
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• pp.108-116
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• 1985

This study was designed to investigate the nutritional anemic state and hematological findings of children, aged 11 and 12 years, living in Haenam Koon (living at home) and in Mokpo City (living in an orphan asylum) located in Chonnam. The mean red blood cell number of male was higher than female in both groups of living in the rural area and in the city orphan asylum (p<0.01), but the white blood cell count was not significantly different. The levels of average hemoglobin, hematocrit and mean corpuscular hemoglobin concentration of the children in both groups were similar, and 19.8% of children living at home in the rural area and 32.1% of children living in the city orphan asylum were anemic. The mean levels of serum total protein, albumin and A/G ratio in children of both group were not statistically different, and 18.4% of children living at home in the rural area and 13.2% of children living in the city orphan asylum were insufficient in the serum total protein value. The average serum cholesterol level of children living at home in the rural area was higher than that of children living in the city orphan asylum(p<0.01), and that of female living at home was higher than that of male (p<0.05). The mean levels of serum iron, total iron binding capacity and transferrin saturation of children living at home in the rural area were significantly higher than those of children living in the city orphan asylum (p<0.01).

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.54-62
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• 2005

While genomics (the set of experimental and computational tools that allows the blueprints of life to be read) opens the doors to a more rational approach to the design and use of living cells to bring about desirable chemical transformations, genomics is, by itself, insufficient. We need tools that allow us to relate genomic and molecular information to cellular physiology and then to the response of a population of cells. We propose the development of hybrid computer cellular models. In such models genomics and chemical detail for a cellular subsystem (e.g. pathogenesis) is embedded in a coarse-grain cell model. Such a construct allows the quantitative and explicit linkage of genomic detail to cell physiology to the extracellular environment. To illustrate the principles involved we are constructing a model for a minimal cell. A minimal cell is a bacterial cell with the fewest number of genes necessary to sustain life as a free living microbe.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권3호
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• pp.178-184
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• 2002

High-throughput screening has become a popular method used to identify new “leads”for potentially therapeutic compounds. Further screening of these lead compounds is typically done with secondary assays which may utilize living, functioning cells as screening tools. A problem (or benefit) with these cell-based assays is that living cells are very sensitive to their environment. We have been interested in the process of stem cell migration and how it relates to the cellular therapy of bone marrow transplantation. In this study we describe a secondary, cell-based assay for screening the effects of various in-vitro conditions on Immature Hematopoietic Cell (IHC) migration. Our results have revealed many subtle factors, such as the cell's adhesive characteristics, or the effect of a culture's growth phase, that need to be accounted for in a screening protocol. Finally, we show that exponentially glowing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly suggest that KG1a cells secrete a chemokinetic factor during the exponential growth phase of a culture.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 1998년도 The Third Asian Fuzzy Systems Symposium
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• pp.158-163
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• 1998

This paper is our first attempt to construct a information processing system such as the living creatures' brain based on artificial life technique. In this paper, we propose a method of constructing neural networks using bio-inspired emergent and evolutionary concept, Ontogeny of living things is realized by cellular automata model and Phylogeny that is living things adaptation ability themselves to given environment, are realized by evolutionary algorithms. Proposing evolving cellular automata neural systems are calledin a word ECANS. A basic component of ECANS is 'cell' which is modeled on chaotic neuron with complex characteristics, In our system, the states of cell are classified into eight by method of connection neighborhood cells. When a problem is given, ECANS adapt itself to the problem by evolutionary method. For fixed cells transition rule, the structure of neural network is adapted by change of initial cell' arrangement. This initial cell is to become a network b developmental process. The effectiveness and the capability of proposed scheme are verified by applying it to pattern classification and robot control problem.