Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Effect of Locally Applied Keratinase on Thickness of Rat Skin

국소도포한 각질분해효소가 흰쥐피부의 두께에 미치는 효과

  • Hwang, Kun (Department of Plastic Surgery, College of Medicine, Inha University, Incheon) ;
  • Chang, Chung-Soon (Department of Biochemistry, College of Medicine, Inha University) ;
  • Kim, Dae Joong (Department of Anatomy, College of Medicine, Inha University) ;
  • Kim, Sung (Department of Plastic Surgery, College of Medicine, Inha University, Incheon) ;
  • Joo, Han Seung (Department of Biochemistry, College of Medicine, Inha University) ;
  • Lee, Seung Jin (C.F Co., Ltd.)
  • 황건 (인하대학교 의과대학 성형외과학교실) ;
  • 장정순 (인하대학교 의과대학 생화학교실) ;
  • 김대중 (인하대학교 의과대학 해부학교실) ;
  • 김성 (인하대학교 의과대학 성형외과학교실) ;
  • 주한승 (인하대학교 의과대학 생화학교실) ;
  • 이승진 (씨. 에프(주))
  • Received : 2005.01.24
  • Published : 2005.05.10
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Abstract

The aim of this study is to elucidate the effect of keratinase on epidermis of rat skin. Twenty-five male Sprague-Dolly rats were used. The hair on the back were removed and $2{\times}2cm$ area was marked. The rats were divided five groups; 1) Control group(Co), 2) Cleansing gel group(Cl), 3) Cleansing gel+keratinase group, 4) Exfoliant gel group(Ex), and 5) Exfoliant gel+ keratinase group(Ex+K). The solutions were applied to the back area twice a day for five days. On fifth day, the skins were harvested, fixed and prepared for histologic sections. The thickness of keratin layer, living epidermis, dermis, and cell layer number of living epidermis were measured. In the group containing keratinase(Cl+K, Ex+K), the thickness of keratin layer and living layer were thinner than other groups. However, there were no significant differences of the cell layer number of living epidermis and thickness of the dermis among the five groups. We think the keratinase may have the effect thinning the keratin layer as well as the thickness of living epidermis, without effecting the living cell and dermal component. The keratinase containing soap may be of benefit to remove the excess keratin layers in human.

Keywords

Acknowledgement

Supported by : 중소기업청

References

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