• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.57-62
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• 2002

Cationic liposomes complexed with DNA have been extensively utilized for the delivery of reporter or therapeutic genes both in culture and in vivo. We investigated and determined the optimum conditions of a cationic liposome, composed of dimethyldioctadecy-lammonium bromide (DDAB) and dioleoyl phosphati-dylethanolamine UOPE), mediated a reporter plasmid expressing luciferase into insect cell lines (Sf-21 and Bm-N) and silkworm larvae. Together the data demonstrated that Bombyx mori nuclear polyhedrosis virus (BmNPV) genomic DNA (128 kb) was successfully transfected into Bm-5 cells using this liposome. These results suggest that DDAB/DOPE liposome will be useful as delivery agents for gene transfer to insect cells both in vitro and in vivo.

• Journal of Pharmaceutical Investigation
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• v.31 no.3
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• pp.197-200
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• 2001

Lectin-conjugated ellagitannin (LET), a newly introduced melanoma-specific antitumor agent which has been synthesized by conjugation of wheat germ agglutinin as a lectin with praecoxin A as an ellagitannin, was encapsulated into sterically stabilized liposomes (SSL). Modified Folin phenol method was established for the quantitation of LET contents in liposomal formulations protein employing the standard calibration curve with bovine serum albumin. After removal of phospholipid by organic solvent extraction, which interferes the specific selectivity of the Folin-Ciocalteu reagent with the protein, recovery of LET was $94.5{\pm}2.3%$ and the encapsulation efficiency was revealed as $37.8{\pm}5.9%$ for 2.5 mg/ml LET solution.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.427.2-428
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• 2002

Previously we formulated new cationic liposomes, DDC, composed of DOTAP. DOPE, and cholesterol (Chol) in 1 : 0.7 : 0.3 molar ratios, and showed that DDC efficiently deliver the plasmid DNA into ovarian cancer cell lines. Here, wild type p53 DNA was transfected into ovarian cancer cells, using the DOC as a nonviral vector and the expression and activity of p53 gene were evaluated in vitro and in vivo. The complexes of plasmid DNA (pp53-EGFP) and DDC were transfected into OVCAR-3 cells. The gene expression was determined by RT -PCR and western blot analysis. (omitted)

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.425.3-426
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• 2002

Cationic lipids have been used as one of the major components for making most promising non-viral gene delivery systems. whereas sodium cholate. an edge activator has been used as a surfactant in making ultradeformable and ultraflexible liposomes called Transfersomes. Using both a cationic lipid, DOTAP and sodium cholate. a newly formulated ultradeformable cationic liposome has been prepared. The average particle size of this formulation was approximately 80nm. (omitted)

• YAKHAK HOEJI
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• v.32 no.4
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• pp.234-238
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• 1988

Proliposome of Sudan IV was prepared according to Payne et al. and evaluated for it's size distribution, surface characteristics and conversion to liposome in aqueous medium. The manufacturing procedures for proliposomes involve the coating of phospholipid solution with Sudan IV on the surface of sorbitol particle in rotary vacuum evaporator. As a result, dry, free flowing and stable proliposome was obtained and multi-lamellar liposome of sudan IV was formed spontaneously when water were added to this. Proliposomes were expected as a probable answer for the physical instability of conventional liposomes.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.206-207
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• 2000

A disposable, electro-chemiluminescent immunosensor utilizing a screen-printed carbon electrode and liposome coupled to antibody as tracer has been constructed. In proportion to the analyte (Legionella species as a model) concentration, the analyte-immunoliposome complexes were transferred by the capillary action through a membrane strip to the electrode, the liposomes were lysed in the presence of detergent, and ruthenium was released for signal generation. Such performance of the immunosensor was appropriate for a point-of-care testing.

• Journal of Environmental Science International
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• v.12 no.1
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• pp.77-80
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• 2003

The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an $\alpha$-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic $\alpha$-helix of MP-B critically affected the biological activity and helical content.

• BMB Reports
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• v.37 no.5
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• pp.515-521
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• 2004

The tricarboxylate (or citrate) carrier was purified from eel liver mitochondria and functionally reconstituted into liposomes. Incubation of the proteoliposomes with various sulfhydryl reagents led to inhibition of the reconstituted citrate transport activity. Preincubation of the proteoliposomes with reversible SH reagents, such as mercurials and methanethiosulfonates, protected the eel liver tricarboxylate carrier against inactivation by the irreversible reagent N-(1-pyrenyl)maleimide (PM). Citrate and L-malate, two substrates of the tricarboxylate carrier, protected the protein against inactivation by sulfhydryl reagents and decreased the fluorescent PM bound to the purified protein. These results suggest that the eel liver tricarboxylate carrier requires a single population of free cysteine(s) in order to manifest catalytic activity. The reactive cysteine(s) is most probably located at or near the substrate binding site of the carrier protein.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.199-201
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• 1985

Plasmids, YEp13 and pMA56, were encapsulated Into liposomes by two different procedures during which the plasmid DNAs were exposed ether to 6$0^{\circ}C$ for 1.5 hr or to sonication for 2-5 min at 4$^{\circ}C$. The encapsulated plasmids were then reextracted and their physical conformations and transformation abilities were examined. It was confirmed from the results that both plasmid DNAs were remained stable throughout the procedures of encapsulation into 1iposomes.

• Applied Science and Convergence Technology
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• v.24 no.6
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• pp.284-288
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• 2015

Anionic small molecules are hard to penetrate the cell membranes because of their negative charges. Encapsulation of small molecules into liposome particles can provide target specific delivery of them. In our previous study, siRNA could be efficiently encapsulated into liposome particles using an asymmetric preparation method of liposomes. In this study, the same method was applied for encapsulation of small anionic fluorescent chemicals such as calcein and indocyanine green (ICG). More than 90% fluorescent chemicals were encapsulated in the asymmetric liposome particles (ALPs). No intracellular fluorescent signal was observed in the tumor cells treated with the unmodified calcein/ALPs and ICG/ALPs, whereas the surface modification with a cell-penetrating polyarginine peptide (R8 or R12) allows cellular uptake of the ALPs. The results demonstrate that the ALPs encapsulating small anionic drugs will be useful for target-specific delivery after modification of target-specific ligands.